Miyakogusa Predicted Gene

Lj4g3v0340760.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0340760.1 Non Characterized Hit- tr|I1K2P8|I1K2P8_SOYBN
Uncharacterized protein OS=Glycine max PE=4
SV=1,89.3,0,COPIIcoated_ERV,Domain of unknown function DUF1692;
ERGIC_N,NULL; seg,NULL; SUBFAMILY NOT NAMED,NULL,CUFF.46994.1
         (384 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g094255.1 | endoplasmic reticulum vesicle transporter | HC...   676   0.0  
Medtr5g062720.1 | endoplasmic reticulum vesicle transporter | HC...   558   e-159
Medtr4g094255.2 | endoplasmic reticulum vesicle transporter | HC...   557   e-159
Medtr5g062720.2 | endoplasmic reticulum vesicle transporter | HC...   433   e-121
Medtr3g081480.1 | endoplasmic reticulum vesicle transporter | HC...   210   2e-54
Medtr2g083260.1 | protein disulfide-isomerase 5-3 | HC | chr2:34...    82   7e-16
Medtr4g080800.1 | protein disulfide-isomerase 5-3 | HC | chr4:31...    79   5e-15

>Medtr4g094255.1 | endoplasmic reticulum vesicle transporter | HC |
           chr4:37548119-37556077 | 20130731
          Length = 384

 Score =  676 bits (1744), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 321/383 (83%), Positives = 344/383 (89%)

Query: 1   MDKIFNKLRNLDAYPKVNEDFYNRTLXXXXXXXXXXXXMXXXXXXXXXXXXXTVTESKLL 60
           MDK+FNKLRNLDAYPKVNEDFYNRTL            M             TVTESKLL
Sbjct: 1   MDKVFNKLRNLDAYPKVNEDFYNRTLAGGVVTVVSAAVMLFLFISELRLYLYTVTESKLL 60

Query: 61  VDTSRGETLHINFDVTFPAVRCSILSLDAMDISGEQHLDIRHNIMKKRIDANGNVIEVKK 120
           VDTSRGETL+INFDVTFPAVRCSILSLD MDISGE+H DI HNIMK+RIDANG VIE +K
Sbjct: 61  VDTSRGETLNINFDVTFPAVRCSILSLDTMDISGERHHDILHNIMKQRIDANGKVIEARK 120

Query: 121 DGIGAAKIERPLQKHGGRLGHDEKYCGSCFGAEESEDHCCNSCEEVREAYRKKGWAMTNM 180
           +GIGA KIERPLQKHGGRL HDEKYCGSCFGAEES+DHCCN+CEEVREAYRKKGWA+TN+
Sbjct: 121 EGIGAPKIERPLQKHGGRLEHDEKYCGSCFGAEESDDHCCNNCEEVREAYRKKGWALTNI 180

Query: 181 DLIDQCEREGFVQKVKDEEGEGCNIHGSLEVNKVAGNFHFATGKSFLQSAIFLTDLLALQ 240
           DLIDQC+REGFVQKVKDEEGEGCNIHGSLEVNKVAGNFHFATG+SFLQSAIFL DLLALQ
Sbjct: 181 DLIDQCQREGFVQKVKDEEGEGCNIHGSLEVNKVAGNFHFATGQSFLQSAIFLADLLALQ 240

Query: 241 DNHYNISHKINKISFGDHYPGLVNPLDGVNWVQGPTHGVYQYFVKVVPTIYTDIRGRVIR 300
           DNHYNISH+INK+SFG HYPGLVNPLDG+ WVQG  HG+YQYF+KVVPT+YTDIRGRVI 
Sbjct: 241 DNHYNISHQINKLSFGHHYPGLVNPLDGIKWVQGNDHGMYQYFIKVVPTVYTDIRGRVIH 300

Query: 301 SNQYSVTEHFKSSELGIAIPGVFFFYDISPIKVSFKEEHIPFLHFLTNVCAIIGGIFTVA 360
           SNQYSVTEHFKSSELG A+PGVFFFYDISPIKV+FKEEHIPFLHFLTN+CAIIGGIFT+A
Sbjct: 301 SNQYSVTEHFKSSELGAAVPGVFFFYDISPIKVNFKEEHIPFLHFLTNICAIIGGIFTIA 360

Query: 361 GIVDSSIYYGQRTIKKKMEIGKY 383
           GIVDSSIYYGQ+TIKKKMEIGKY
Sbjct: 361 GIVDSSIYYGQKTIKKKMEIGKY 383


>Medtr5g062720.1 | endoplasmic reticulum vesicle transporter | HC |
           chr5:26005878-25996809 | 20130731
          Length = 386

 Score =  558 bits (1437), Expect = e-159,   Method: Compositional matrix adjust.
 Identities = 254/385 (65%), Positives = 318/385 (82%), Gaps = 2/385 (0%)

Query: 1   MDKIFNKLRNLDAYPKVNEDFYNRTLXXXXXXXXXXXXMXXXXXXXXXXXXXTVTESKLL 60
           MD I NKLRNLDAYPK+NEDFY+RTL            M               TE+KL+
Sbjct: 1   MDSIMNKLRNLDAYPKINEDFYSRTLSGGLITIVSSILMLLLFFSELRLYLHAATETKLV 60

Query: 61  VDTSRGETLHINFDVTFPAVRCSILSLDAMDISGEQHLDIRHNIMKKRIDANGNVIEVKK 120
           VDTSRGETL INFDVTFPA+ CSI+SLDAMDISGEQHLD+RH+I+KKRID++GNVIE ++
Sbjct: 61  VDTSRGETLRINFDVTFPALACSIVSLDAMDISGEQHLDVRHDIIKKRIDSHGNVIETRQ 120

Query: 121 DGIGAAKIERPLQKHGGRLGHDEKYCGSCFGAEESEDHCCNSCEEVREAYRKKGWAMTNM 180
           DGIG+  IE+PLQ+HGGRL H+E YCGSC+GAE S++ CCNSCEEVREAYRKKGWA+++ 
Sbjct: 121 DGIGSPNIEKPLQRHGGRLEHNETYCGSCYGAEASDEECCNSCEEVREAYRKKGWALSSP 180

Query: 181 DLIDQCEREGFVQKVKDEEGEGCNIHGSLEVNKVAGNFHFATGKSFLQSAIFLTDLLALQ 240
           D IDQC+REGF++++K+EEGEGCN++G LEVNKVAGNFHFA GKSF QS + + DLLA Q
Sbjct: 181 DSIDQCKREGFLERIKEEEGEGCNVYGFLEVNKVAGNFHFAPGKSFQQSGVHVHDLLAFQ 240

Query: 241 DNHYNISHKINKISFGDHYPGLVNPLDGVNWVQGPTHGVYQYFVKVVPTIYTDIRGRVIR 300
              +N+SH IN+I+FGD++PG+VNPLD V+W Q    G+YQYF+KVVPT+YTD+ G  I+
Sbjct: 241 KESFNLSHHINRIAFGDYFPGVVNPLDRVHWTQETPSGMYQYFIKVVPTMYTDVSGNTIQ 300

Query: 301 SNQYSVTEHFKSSELG--IAIPGVFFFYDISPIKVSFKEEHIPFLHFLTNVCAIIGGIFT 358
           SNQ+SVTEHF+++++G   ++PGVFFFYD+SPIKV+F EEH+ FLHFLTNVCAI+GGIFT
Sbjct: 301 SNQFSVTEHFRTADVGRLQSLPGVFFFYDLSPIKVTFTEEHVSFLHFLTNVCAIVGGIFT 360

Query: 359 VAGIVDSSIYYGQRTIKKKMEIGKY 383
           V+GI+DS IY+GQ+ IKKKME+GK+
Sbjct: 361 VSGILDSFIYHGQKAIKKKMELGKF 385


>Medtr4g094255.2 | endoplasmic reticulum vesicle transporter | HC |
           chr4:37548132-37556077 | 20130731
          Length = 295

 Score =  557 bits (1435), Expect = e-159,   Method: Compositional matrix adjust.
 Identities = 260/294 (88%), Positives = 281/294 (95%)

Query: 90  MDISGEQHLDIRHNIMKKRIDANGNVIEVKKDGIGAAKIERPLQKHGGRLGHDEKYCGSC 149
           MDISGE+H DI HNIMK+RIDANG VIE +K+GIGA KIERPLQKHGGRL HDEKYCGSC
Sbjct: 1   MDISGERHHDILHNIMKQRIDANGKVIEARKEGIGAPKIERPLQKHGGRLEHDEKYCGSC 60

Query: 150 FGAEESEDHCCNSCEEVREAYRKKGWAMTNMDLIDQCEREGFVQKVKDEEGEGCNIHGSL 209
           FGAEES+DHCCN+CEEVREAYRKKGWA+TN+DLIDQC+REGFVQKVKDEEGEGCNIHGSL
Sbjct: 61  FGAEESDDHCCNNCEEVREAYRKKGWALTNIDLIDQCQREGFVQKVKDEEGEGCNIHGSL 120

Query: 210 EVNKVAGNFHFATGKSFLQSAIFLTDLLALQDNHYNISHKINKISFGDHYPGLVNPLDGV 269
           EVNKVAGNFHFATG+SFLQSAIFL DLLALQDNHYNISH+INK+SFG HYPGLVNPLDG+
Sbjct: 121 EVNKVAGNFHFATGQSFLQSAIFLADLLALQDNHYNISHQINKLSFGHHYPGLVNPLDGI 180

Query: 270 NWVQGPTHGVYQYFVKVVPTIYTDIRGRVIRSNQYSVTEHFKSSELGIAIPGVFFFYDIS 329
            WVQG  HG+YQYF+KVVPT+YTDIRGRVI SNQYSVTEHFKSSELG A+PGVFFFYDIS
Sbjct: 181 KWVQGNDHGMYQYFIKVVPTVYTDIRGRVIHSNQYSVTEHFKSSELGAAVPGVFFFYDIS 240

Query: 330 PIKVSFKEEHIPFLHFLTNVCAIIGGIFTVAGIVDSSIYYGQRTIKKKMEIGKY 383
           PIKV+FKEEHIPFLHFLTN+CAIIGGIFT+AGIVDSSIYYGQ+TIKKKMEIGKY
Sbjct: 241 PIKVNFKEEHIPFLHFLTNICAIIGGIFTIAGIVDSSIYYGQKTIKKKMEIGKY 294


>Medtr5g062720.2 | endoplasmic reticulum vesicle transporter | HC |
           chr5:26005878-25997434 | 20130731
          Length = 312

 Score =  433 bits (1113), Expect = e-121,   Method: Compositional matrix adjust.
 Identities = 197/303 (65%), Positives = 243/303 (80%)

Query: 1   MDKIFNKLRNLDAYPKVNEDFYNRTLXXXXXXXXXXXXMXXXXXXXXXXXXXTVTESKLL 60
           MD I NKLRNLDAYPK+NEDFY+RTL            M               TE+KL+
Sbjct: 1   MDSIMNKLRNLDAYPKINEDFYSRTLSGGLITIVSSILMLLLFFSELRLYLHAATETKLV 60

Query: 61  VDTSRGETLHINFDVTFPAVRCSILSLDAMDISGEQHLDIRHNIMKKRIDANGNVIEVKK 120
           VDTSRGETL INFDVTFPA+ CSI+SLDAMDISGEQHLD+RH+I+KKRID++GNVIE ++
Sbjct: 61  VDTSRGETLRINFDVTFPALACSIVSLDAMDISGEQHLDVRHDIIKKRIDSHGNVIETRQ 120

Query: 121 DGIGAAKIERPLQKHGGRLGHDEKYCGSCFGAEESEDHCCNSCEEVREAYRKKGWAMTNM 180
           DGIG+  IE+PLQ+HGGRL H+E YCGSC+GAE S++ CCNSCEEVREAYRKKGWA+++ 
Sbjct: 121 DGIGSPNIEKPLQRHGGRLEHNETYCGSCYGAEASDEECCNSCEEVREAYRKKGWALSSP 180

Query: 181 DLIDQCEREGFVQKVKDEEGEGCNIHGSLEVNKVAGNFHFATGKSFLQSAIFLTDLLALQ 240
           D IDQC+REGF++++K+EEGEGCN++G LEVNKVAGNFHFA GKSF QS + + DLLA Q
Sbjct: 181 DSIDQCKREGFLERIKEEEGEGCNVYGFLEVNKVAGNFHFAPGKSFQQSGVHVHDLLAFQ 240

Query: 241 DNHYNISHKINKISFGDHYPGLVNPLDGVNWVQGPTHGVYQYFVKVVPTIYTDIRGRVIR 300
              +N+SH IN+I+FGD++PG+VNPLD V+W Q    G+YQYF+KVVPT+YTD+ G  I+
Sbjct: 241 KESFNLSHHINRIAFGDYFPGVVNPLDRVHWTQETPSGMYQYFIKVVPTMYTDVSGNTIQ 300

Query: 301 SNQ 303
           SNQ
Sbjct: 301 SNQ 303


>Medtr3g081480.1 | endoplasmic reticulum vesicle transporter | HC |
           chr3:36886778-36893954 | 20130731
          Length = 355

 Score =  210 bits (534), Expect = 2e-54,   Method: Compositional matrix adjust.
 Identities = 122/365 (33%), Positives = 199/365 (54%), Gaps = 33/365 (9%)

Query: 8   LRNLDAYPKVNEDFYNRTLXXXXXXXXXXXXMXXXXXXXXXXXXXTVTESKLLVDTSRGE 67
           ++NLDA+P+  +    +T             M             T T  ++ VD  RGE
Sbjct: 7   IKNLDAFPRTEDHLLQKTQSGALVSIIGLIIMATLFLHELGYYLTTYTVHQMSVDLKRGE 66

Query: 68  TLHINFDVTFPAVRCSILSLDAMDISGEQHLDIRHNIMKKRIDANGNVIEVKKDGIGAAK 127
           TL I+ ++TFP++ C +LS+DA+D+SG+  +D+  NI K R+++ G +I       G   
Sbjct: 67  TLPIHINMTFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNSYGQII-------GTEY 119

Query: 128 IERPLQKHGGRLGHDEKYCGSCFGAEESEDHCCNSCEEVREAYRKKGWAMTNMDLIDQCE 187
           I   ++K     GH+  +       ++S+DH  +S ++V            ++   D+  
Sbjct: 120 ISDLVEK-----GHEHDHDHGTHKHDDSKDHHEHSEQKV------------HLQTFDEAT 162

Query: 188 REGFVQKVKD--EEGEGCNIHGSLEVNKVAGNFHFATGKSFLQSAIFLTDLLALQDNHYN 245
            E  ++KVK+  + GEGC ++G L+V +VAGNFH     S     I++  ++     + N
Sbjct: 163 -ENTIKKVKEALKNGEGCRVYGVLDVQRVAGNFHI----SVHGLNIYVAQMIFDAGKNVN 217

Query: 246 ISHKINKISFGDHYPGLVNPLDGVNWVQGPTHGVYQYFVKVVPTIYTDIRGRVIRSNQYS 305
           +SH I+ +SFG  YPG+ NPLD  + +     G ++Y++K+VPT Y  I   V+ +NQ+S
Sbjct: 218 VSHVIHDLSFGPKYPGIHNPLDETSRILHDASGTFKYYIKIVPTEYRYISKEVLPTNQFS 277

Query: 306 VTEHFK--SSELGIAIPGVFFFYDISPIKVSFKEEHIPFLHFLTNVCAIIGGIFTVAGIV 363
           VTE+F   +S+     P V+F YD+SPI V+ KEE   FLHF+T +CA++GG F V G++
Sbjct: 278 VTEYFSPITSQFDRTWPAVYFLYDLSPITVTIKEERRSFLHFITRLCAVLGGTFAVTGML 337

Query: 364 DSSIY 368
           D  +Y
Sbjct: 338 DRWMY 342


>Medtr2g083260.1 | protein disulfide-isomerase 5-3 | HC |
           chr2:34960893-34954393 | 20130731
          Length = 480

 Score = 82.4 bits (202), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 59/199 (29%), Positives = 99/199 (49%), Gaps = 31/199 (15%)

Query: 202 GCNIHGSLEVNKVAGNFHFATGKSFLQSAIFLTDLLALQDNHYNISHKINKISFGDHY-P 260
           GC I G + V KV GN   +            +D  +   +  N+SH ++ +SFG    P
Sbjct: 293 GCRIEGYVRVKKVPGNLIISAR----------SDAHSFDASQMNMSHAVHHLSFGKKLSP 342

Query: 261 GLV--------------NPLDGVNWVQGPTHG---VYQYFVKVVPTIYTDIRGRVIRSNQ 303
            L+              + LDG++++     G     ++++++V T     +G  +   +
Sbjct: 343 KLMSDVQRLIPYVGNSHDRLDGLSFINSHDFGANVTLEHYLQIVKTEVITRQGYQL-VEE 401

Query: 304 YSVTEHFKSSELGIAIPGVFFFYDISPIKVSFKEEHIPFLHFLTNVCAIIGGIFTVAGIV 363
           Y  T H  S    + +P   F   +SP++V   E+H  F HF+TNVCAI+GG+FTVAGI 
Sbjct: 402 YEYTAH-SSLAHSLHVPVARFHLQLSPMQVLITEDHKSFSHFITNVCAIVGGVFTVAGIT 460

Query: 364 DSSIYYGQRTIKKKMEIGK 382
           +S ++   R + +K+E+GK
Sbjct: 461 ESILHNTIR-LMRKVELGK 478


>Medtr4g080800.1 | protein disulfide-isomerase 5-3 | HC |
           chr4:31384571-31393311 | 20130731
          Length = 477

 Score = 79.3 bits (194), Expect = 5e-15,   Method: Compositional matrix adjust.
 Identities = 69/205 (33%), Positives = 99/205 (48%), Gaps = 43/205 (20%)

Query: 202 GCNIHGSLEVNKVAGNFHFATGKSFLQSAIFLTDLLALQDNHYNISHKINKISFGDHYPG 261
           GC + G + V KV G        S + SA   +D  +   +  N+SH IN +SFG     
Sbjct: 290 GCRVEGYVRVKKVPG--------SLVVSA--RSDAHSFDASQMNMSHVINHLSFGKK--- 336

Query: 262 LVNP---LDGVNWVQ--GPTHG-----------------VYQYFVKVVPTIYTDIRGRVI 299
            V P   +D  +W+   G  H                    +++++VV T     +G  +
Sbjct: 337 -VTPRAMIDVKHWIPYLGINHDRLNGRSFINTRDLEGNVTIEHYIQVVKTEVITRKGYKL 395

Query: 300 RSNQYSVTEHFKSSELGIAIPGVFFFYDISPIKVSFKEEHIPFLHFLTNVCAIIGGIFTV 359
              +Y  T H  S    + IP   F  ++SP++V   E    F HF+TNVCAIIGG+FTV
Sbjct: 396 -IEEYEYTAH-SSVAHSVNIPVARFHLELSPMQVLITENQKSFSHFITNVCAIIGGVFTV 453

Query: 360 AGIVDSSIYYGQRTIK--KKMEIGK 382
           AGI+DS ++    TIK  KK+EIGK
Sbjct: 454 AGILDSILH---NTIKAMKKIEIGK 475



 Score = 49.7 bits (117), Expect = 5e-06,   Method: Compositional matrix adjust.
 Identities = 29/107 (27%), Positives = 49/107 (45%), Gaps = 1/107 (0%)

Query: 6   NKLRNLDAYPKVNEDFYNRTLXXXXXXXXXXXXMXXXXXXXXXXXXXTVTESKLLVD-TS 64
           +KL+++D Y K+  D    +L            M               T + ++VD +S
Sbjct: 5   SKLKSVDFYRKIPRDLTEASLSGAGLSILAALAMMFLFGMELSNYFAVTTSTSVIVDKSS 64

Query: 65  RGETLHINFDVTFPAVRCSILSLDAMDISGEQHLDIRHNIMKKRIDA 111
            G+ L I+F+ +FPA+ C   S+D  D+ G   L+I   + K  ID+
Sbjct: 65  DGDFLRIDFNFSFPALSCEFASVDVSDVLGTNRLNITKTVRKFSIDS 111