Miyakogusa Predicted Gene

Lj4g3v0049640.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0049640.1 Non Characterized Hit- tr|I1KNQ6|I1KNQ6_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.42426
PE,83.97,0,GTP-BINDING PROTEIN ENGA,GTP-binding protein EngA;
MSS1/TRME-RELATED GTP-BINDING PROTEIN,NULL; P-loo,CUFF.46576.1
         (387 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g134500.1 | ribosome-associated GTPase EngA | HC | chr4:56...   588   e-168
Medtr4g065030.1 | GTPase Der protein | HC | chr4:24379001-243866...   140   2e-33
Medtr4g065030.3 | GTPase Der protein | HC | chr4:24379001-243866...   140   2e-33
Medtr4g065030.2 | GTPase Der protein | HC | chr4:24379001-243866...   140   2e-33
Medtr3g073790.1 | tRNA modification GTPase trmE | HC | chr3:3330...    60   4e-09

>Medtr4g134500.1 | ribosome-associated GTPase EngA | HC |
           chr4:56328156-56322710 | 20130731
          Length = 545

 Score =  588 bits (1516), Expect = e-168,   Method: Compositional matrix adjust.
 Identities = 288/384 (75%), Positives = 323/384 (84%), Gaps = 8/384 (2%)

Query: 1   MNKSEXXXXXXXXXXXXGNEMCRLGFGDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLN 60
           MNKSE             NEM RLGFGDPIAISAETGLGMHDL+LSL+P+LEDYML    
Sbjct: 158 MNKSESLFDVDGSLASSANEMSRLGFGDPIAISAETGLGMHDLFLSLQPVLEDYML---- 213

Query: 61  VNDEGAQENSNSEDTSSPEAEESKLPLQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGL 120
             +E A+EN+  +++S PEA+ESKLPLQLAIVGRPNVGKSTL+NTLLQEDRVLVGPEAGL
Sbjct: 214 --NESAKENTCGDESSFPEADESKLPLQLAIVGRPNVGKSTLLNTLLQEDRVLVGPEAGL 271

Query: 121 TRDSIRTQFEFEGRTIYLVDTAGWLQRTKQEKGAASLSIMQSRKSLLRAHVVALVLDAEE 180
           TRD+IRTQFEF+GRTIYLVDTAGWLQRTK EKGA+SLSIMQSRKSLLRAH++ALVLDA E
Sbjct: 272 TRDAIRTQFEFQGRTIYLVDTAGWLQRTKLEKGASSLSIMQSRKSLLRAHIIALVLDAGE 331

Query: 181 IINERRSMKHAEVVIARRAVEEGRGLVVIVNKMDLLRG--KASSFEKVMXXXXXXXXXXX 238
           I++ +RSMKHAEVVIARRAVEEGRGLVVIVNKMDLLRG  K++S+E+V+           
Sbjct: 332 IVSAKRSMKHAEVVIARRAVEEGRGLVVIVNKMDLLRGKNKSASYEQVLEAVQQEIQTII 391

Query: 239 XXXXXXXXXFTSALEGRGRTAVLEQVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQAA 298
                    F SALEGRGRT VL QVIDTYEKWC+RLSTARLNRW+QKVMSRHSWKDQAA
Sbjct: 392 PQVTGIPVVFISALEGRGRTTVLHQVIDTYEKWCTRLSTARLNRWLQKVMSRHSWKDQAA 451

Query: 299 QPKIKYFTQVKARPPTFVAFLRGKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKK 358
           QPKIKYFTQVKARPPTFVAF+ GKT+L+DTD RFLTKSLK+DFDLGGIPIRIMQR++ KK
Sbjct: 452 QPKIKYFTQVKARPPTFVAFVHGKTKLSDTDTRFLTKSLKDDFDLGGIPIRIMQRSIAKK 511

Query: 359 EVSGNSKSGHSAGRVAERMMSDKR 382
           +V G+SKS H  GRVA R+ SDKR
Sbjct: 512 DVGGSSKSSHPIGRVALRIKSDKR 535


>Medtr4g065030.1 | GTPase Der protein | HC | chr4:24379001-24386634
           | 20130731
          Length = 646

 Score =  140 bits (353), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 111/374 (29%), Positives = 186/374 (49%), Gaps = 39/374 (10%)

Query: 19  NEMCRLGFGDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSP 78
           +E   LG  +PI ISA +G G  DL               L++   G Q+    E+  + 
Sbjct: 299 SEFWSLGL-EPIPISAISGTGTGDL---------------LDLVCSGLQK---VEEPQNL 339

Query: 79  EAEESKLPLQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFE-FEGRTIY 137
             EE  +P  ++IVGRPNVGKS+++N L+ EDR +V P +G TRD+I T+F   +G+  +
Sbjct: 340 VEEEDYVP-AISIVGRPNVGKSSILNALVGEDRTIVSPISGTTRDAIDTEFTGPDGQKFH 398

Query: 138 LVDTAGWLQRTKQEKGAAS---LSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVV 194
           L+DTAG  +RT      ++   LS+ ++ +++ R+ VVALV++A   I E+      +  
Sbjct: 399 LIDTAGIRKRTTVASAGSTTEALSVNRAFRAIRRSDVVALVIEAMACITEQ------DYK 452

Query: 195 IARRAVEEGRGLVVIVNKMDLLRGKASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEG 254
           IA R  +EG+G V++VNK D +  K    ++                      +++AL G
Sbjct: 453 IAERIEKEGKGCVIVVNKWDTIPNKN---QQTALYYEQDVREKLRLLDWAPVVYSTALAG 509

Query: 255 RGRTAVLEQVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQ--AAQPKIKYFTQVKARP 312
           +    ++   I+  ++   RL T+ LN+ +++ ++           + ++ Y TQ   RP
Sbjct: 510 QNVDKIIVAAIEVEKERSRRLGTSILNQVVREAVTFKPPPRTRGGKRGRVYYCTQAAIRP 569

Query: 313 PTFVAFLRGKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGR 372
           PTFV F+       +T  RF+ K L+ +    G PIR++ R+  K E       G  A R
Sbjct: 570 PTFVFFVNDAKLFPETYRRFMEKQLRSNAGFPGTPIRLLWRSRRKME----KDEGKKATR 625

Query: 373 VAERMMSDKRSVLA 386
             E   S +R +++
Sbjct: 626 PKENFASHRRKLIS 639



 Score = 53.5 bits (127), Expect = 3e-07,   Method: Compositional matrix adjust.
 Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 6/100 (6%)

Query: 57  RVLNVNDE--GAQENSNSEDTSSPEAE---ESKLPLQLAIVGRPNVGKSTLMNTLLQEDR 111
           RVL + DE    +E   +   S P+ +   ++ LP ++AIVGRPNVGKS L N L+  ++
Sbjct: 105 RVLTIEDERSDVKETGKNSKRSKPKRKIIPDNLLP-RIAIVGRPNVGKSALFNRLVGGNK 163

Query: 112 VLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGWLQRTKQE 151
            +V  E G+TRD +  +  +      +VDT G L  +K +
Sbjct: 164 AIVVDEPGVTRDRLYGRSYWGDNEFMVVDTGGVLTVSKSQ 203


>Medtr4g065030.3 | GTPase Der protein | HC | chr4:24379001-24386603
           | 20130731
          Length = 646

 Score =  140 bits (353), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 111/374 (29%), Positives = 186/374 (49%), Gaps = 39/374 (10%)

Query: 19  NEMCRLGFGDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSP 78
           +E   LG  +PI ISA +G G  DL               L++   G Q+    E+  + 
Sbjct: 299 SEFWSLGL-EPIPISAISGTGTGDL---------------LDLVCSGLQK---VEEPQNL 339

Query: 79  EAEESKLPLQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFE-FEGRTIY 137
             EE  +P  ++IVGRPNVGKS+++N L+ EDR +V P +G TRD+I T+F   +G+  +
Sbjct: 340 VEEEDYVP-AISIVGRPNVGKSSILNALVGEDRTIVSPISGTTRDAIDTEFTGPDGQKFH 398

Query: 138 LVDTAGWLQRTKQEKGAAS---LSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVV 194
           L+DTAG  +RT      ++   LS+ ++ +++ R+ VVALV++A   I E+      +  
Sbjct: 399 LIDTAGIRKRTTVASAGSTTEALSVNRAFRAIRRSDVVALVIEAMACITEQ------DYK 452

Query: 195 IARRAVEEGRGLVVIVNKMDLLRGKASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEG 254
           IA R  +EG+G V++VNK D +  K    ++                      +++AL G
Sbjct: 453 IAERIEKEGKGCVIVVNKWDTIPNKN---QQTALYYEQDVREKLRLLDWAPVVYSTALAG 509

Query: 255 RGRTAVLEQVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQ--AAQPKIKYFTQVKARP 312
           +    ++   I+  ++   RL T+ LN+ +++ ++           + ++ Y TQ   RP
Sbjct: 510 QNVDKIIVAAIEVEKERSRRLGTSILNQVVREAVTFKPPPRTRGGKRGRVYYCTQAAIRP 569

Query: 313 PTFVAFLRGKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGR 372
           PTFV F+       +T  RF+ K L+ +    G PIR++ R+  K E       G  A R
Sbjct: 570 PTFVFFVNDAKLFPETYRRFMEKQLRSNAGFPGTPIRLLWRSRRKME----KDEGKKATR 625

Query: 373 VAERMMSDKRSVLA 386
             E   S +R +++
Sbjct: 626 PKENFASHRRKLIS 639



 Score = 53.5 bits (127), Expect = 3e-07,   Method: Compositional matrix adjust.
 Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 6/100 (6%)

Query: 57  RVLNVNDE--GAQENSNSEDTSSPEAE---ESKLPLQLAIVGRPNVGKSTLMNTLLQEDR 111
           RVL + DE    +E   +   S P+ +   ++ LP ++AIVGRPNVGKS L N L+  ++
Sbjct: 105 RVLTIEDERSDVKETGKNSKRSKPKRKIIPDNLLP-RIAIVGRPNVGKSALFNRLVGGNK 163

Query: 112 VLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGWLQRTKQE 151
            +V  E G+TRD +  +  +      +VDT G L  +K +
Sbjct: 164 AIVVDEPGVTRDRLYGRSYWGDNEFMVVDTGGVLTVSKSQ 203


>Medtr4g065030.2 | GTPase Der protein | HC | chr4:24379001-24386634
           | 20130731
          Length = 646

 Score =  140 bits (353), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 111/374 (29%), Positives = 186/374 (49%), Gaps = 39/374 (10%)

Query: 19  NEMCRLGFGDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSP 78
           +E   LG  +PI ISA +G G  DL               L++   G Q+    E+  + 
Sbjct: 299 SEFWSLGL-EPIPISAISGTGTGDL---------------LDLVCSGLQK---VEEPQNL 339

Query: 79  EAEESKLPLQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFE-FEGRTIY 137
             EE  +P  ++IVGRPNVGKS+++N L+ EDR +V P +G TRD+I T+F   +G+  +
Sbjct: 340 VEEEDYVP-AISIVGRPNVGKSSILNALVGEDRTIVSPISGTTRDAIDTEFTGPDGQKFH 398

Query: 138 LVDTAGWLQRTKQEKGAAS---LSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVV 194
           L+DTAG  +RT      ++   LS+ ++ +++ R+ VVALV++A   I E+      +  
Sbjct: 399 LIDTAGIRKRTTVASAGSTTEALSVNRAFRAIRRSDVVALVIEAMACITEQ------DYK 452

Query: 195 IARRAVEEGRGLVVIVNKMDLLRGKASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEG 254
           IA R  +EG+G V++VNK D +  K    ++                      +++AL G
Sbjct: 453 IAERIEKEGKGCVIVVNKWDTIPNKN---QQTALYYEQDVREKLRLLDWAPVVYSTALAG 509

Query: 255 RGRTAVLEQVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQ--AAQPKIKYFTQVKARP 312
           +    ++   I+  ++   RL T+ LN+ +++ ++           + ++ Y TQ   RP
Sbjct: 510 QNVDKIIVAAIEVEKERSRRLGTSILNQVVREAVTFKPPPRTRGGKRGRVYYCTQAAIRP 569

Query: 313 PTFVAFLRGKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGR 372
           PTFV F+       +T  RF+ K L+ +    G PIR++ R+  K E       G  A R
Sbjct: 570 PTFVFFVNDAKLFPETYRRFMEKQLRSNAGFPGTPIRLLWRSRRKME----KDEGKKATR 625

Query: 373 VAERMMSDKRSVLA 386
             E   S +R +++
Sbjct: 626 PKENFASHRRKLIS 639



 Score = 53.5 bits (127), Expect = 3e-07,   Method: Compositional matrix adjust.
 Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 6/100 (6%)

Query: 57  RVLNVNDE--GAQENSNSEDTSSPEAE---ESKLPLQLAIVGRPNVGKSTLMNTLLQEDR 111
           RVL + DE    +E   +   S P+ +   ++ LP ++AIVGRPNVGKS L N L+  ++
Sbjct: 105 RVLTIEDERSDVKETGKNSKRSKPKRKIIPDNLLP-RIAIVGRPNVGKSALFNRLVGGNK 163

Query: 112 VLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGWLQRTKQE 151
            +V  E G+TRD +  +  +      +VDT G L  +K +
Sbjct: 164 AIVVDEPGVTRDRLYGRSYWGDNEFMVVDTGGVLTVSKSQ 203


>Medtr3g073790.1 | tRNA modification GTPase trmE | HC |
           chr3:33306093-33311427 | 20130731
          Length = 543

 Score = 60.1 bits (144), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 30/57 (52%), Positives = 37/57 (64%)

Query: 87  LQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAG 143
           LQ+AIVGRPNVGKS+L+N   + +R +V   AG TRD I       G  I L+DTAG
Sbjct: 295 LQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEASININGIPITLLDTAG 351