Miyakogusa Predicted Gene

Lj4g3v0037570.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj4g3v0037570.1 Non Characterized Hit- tr|I1KNQ6|I1KNQ6_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.42426
PE,82.68,0,GTPase_EngA: ribosome-associated GTPase EngA,GTP-binding
protein EngA; small_GTP: small GTP-binding ,CUFF.46562.1
         (563 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g134500.1 | ribosome-associated GTPase EngA | HC | chr4:56...   810   0.0  
Medtr4g065030.1 | GTPase Der protein | HC | chr4:24379001-243866...   205   1e-52
Medtr4g065030.3 | GTPase Der protein | HC | chr4:24379001-243866...   205   1e-52
Medtr4g065030.2 | GTPase Der protein | HC | chr4:24379001-243866...   205   1e-52
Medtr3g073790.1 | tRNA modification GTPase trmE | HC | chr3:3330...    60   7e-09

>Medtr4g134500.1 | ribosome-associated GTPase EngA | HC |
           chr4:56328156-56322710 | 20130731
          Length = 545

 Score =  810 bits (2091), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 404/540 (74%), Positives = 450/540 (83%), Gaps = 13/540 (2%)

Query: 22  IFLFN-SSATATRGFSSTKNKPLDLDFTQLPISSLPTVIIIGRPNVGKSALFNRLIRRRE 80
           IFLF  +  T +RGF + K    +LDFT++ IS LPTVII+GRPNVGKSALFNRLIRRRE
Sbjct: 6   IFLFRWTRRTFSRGFCTKKK---NLDFTKVEISRLPTVIILGRPNVGKSALFNRLIRRRE 62

Query: 81  ALVYNTPEDHVTRDIREGVAKLGNLRFRVLDSAGLXXXXXXXXXILHRTASITANFFAAS 140
           ALVYNTP+DHVTRDIREGVAKLG+LRFRVLDSAGL         ILHRTAS TAN  + S
Sbjct: 63  ALVYNTPDDHVTRDIREGVAKLGHLRFRVLDSAGLEAEATSGS-ILHRTASFTANVLSTS 121

Query: 141 HVALFLTDARAGLHPLDQEVGKWLRKNAPQLKPIVAMNKSEXXXXXXXXXXXXGNEMCRL 200
           H  LFLTDARAGLHPLDQEVGKWLRKNAPQLKPI+ MNKSE             NEM RL
Sbjct: 122 HSLLFLTDARAGLHPLDQEVGKWLRKNAPQLKPILVMNKSESLFDVDGSLASSANEMSRL 181

Query: 201 GFGDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSPEAEESK 260
           GFGDPIAISAETGLGMHDL+LSL+P+LEDYML      +E A+EN+  +++S PEA+ESK
Sbjct: 182 GFGDPIAISAETGLGMHDLFLSLQPVLEDYML------NESAKENTCGDESSFPEADESK 235

Query: 261 LPLQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGW 320
           LPLQLAIVGRPNVGKSTL+NTLLQEDRVLVGPEAGLTRD+IRTQFEF+GRTIYLVDTAGW
Sbjct: 236 LPLQLAIVGRPNVGKSTLLNTLLQEDRVLVGPEAGLTRDAIRTQFEFQGRTIYLVDTAGW 295

Query: 321 LQRTKQEKGAASLSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVVIARRAVEEGR 380
           LQRTK EKGA+SLSIMQSRKSLLRAH++ALVLDA EI++ +RSMKHAEVVIARRAVEEGR
Sbjct: 296 LQRTKLEKGASSLSIMQSRKSLLRAHIIALVLDAGEIVSAKRSMKHAEVVIARRAVEEGR 355

Query: 381 GLVVIVNKMDLLRG--KASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEGRGRTAVLE 438
           GLVVIVNKMDLLRG  K++S+E+V+                    F SALEGRGRT VL 
Sbjct: 356 GLVVIVNKMDLLRGKNKSASYEQVLEAVQQEIQTIIPQVTGIPVVFISALEGRGRTTVLH 415

Query: 439 QVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQAAQPKIKYFTQVKARPPTFVAFLRGK 498
           QVIDTYEKWC+RLSTARLNRW+QKVMSRHSWKDQAAQPKIKYFTQVKARPPTFVAF+ GK
Sbjct: 416 QVIDTYEKWCTRLSTARLNRWLQKVMSRHSWKDQAAQPKIKYFTQVKARPPTFVAFVHGK 475

Query: 499 TQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGRVAERMMSDKR 558
           T+L+DTD RFLTKSLK+DFDLGGIPIRIMQR++ KK+V G+SKS H  GRVA R+ SDKR
Sbjct: 476 TKLSDTDTRFLTKSLKDDFDLGGIPIRIMQRSIAKKDVGGSSKSSHPIGRVALRIKSDKR 535


>Medtr4g065030.1 | GTPase Der protein | HC | chr4:24379001-24386634
           | 20130731
          Length = 646

 Score =  205 bits (521), Expect = 1e-52,   Method: Compositional matrix adjust.
 Identities = 167/546 (30%), Positives = 263/546 (48%), Gaps = 72/546 (13%)

Query: 50  LPISSLPTVIIIGRPNVGKSALFNRLIRRREALVYNTPEDHVTRDIREGVAKLGNLRFRV 109
           +P + LP + I+GRPNVGKSALFNRL+   +A+V + P   VTRD   G +  G+  F V
Sbjct: 133 IPDNLLPRIAIVGRPNVGKSALFNRLVGGNKAIVVDEP--GVTRDRLYGRSYWGDNEFMV 190

Query: 110 LDSAGLXXXXXXXXXILH-----------------RTAS-------ITANFFAA---SHV 142
           +D+ G+         ++                  R A+       I     AA   S V
Sbjct: 191 VDTGGVLTVSKSQTTVMEDLDISTTIGMDGIPLASREAAVARMPSMIERQAIAAVEESPV 250

Query: 143 ALFLTDARAGLHPLDQEVGKWLRKNAPQLKPIVAMNKSEXXXXXXXXXXXXGNEMCRLGF 202
            +FL D +AGL   D E+  WLRKN      I+A+NK E             +E   LG 
Sbjct: 251 IIFLVDGQAGLTAADVEIADWLRKNYSNKSIILAVNKCESPRKRIMQV----SEFWSLGL 306

Query: 203 GDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSPEAEESKLP 262
            +PI ISA +G G  DL               L++   G Q+    E+  +   EE  +P
Sbjct: 307 -EPIPISAISGTGTGDL---------------LDLVCSGLQK---VEEPQNLVEEEDYVP 347

Query: 263 LQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFEF-EGRTIYLVDTAGWL 321
             ++IVGRPNVGKS+++N L+ EDR +V P +G TRD+I T+F   +G+  +L+DTAG  
Sbjct: 348 -AISIVGRPNVGKSSILNALVGEDRTIVSPISGTTRDAIDTEFTGPDGQKFHLIDTAGIR 406

Query: 322 QRTKQEKGAAS---LSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVVIARRAVEE 378
           +RT      ++   LS+ ++ +++ R+ VVALV++A   I E+      +  IA R  +E
Sbjct: 407 KRTTVASAGSTTEALSVNRAFRAIRRSDVVALVIEAMACITEQ------DYKIAERIEKE 460

Query: 379 GRGLVVIVNKMDLLRGKASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEGRGRTAVLE 438
           G+G V++VNK D +  K    ++                      +++AL G+    ++ 
Sbjct: 461 GKGCVIVVNKWDTIPNKN---QQTALYYEQDVREKLRLLDWAPVVYSTALAGQNVDKIIV 517

Query: 439 QVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQ--AAQPKIKYFTQVKARPPTFVAFLR 496
             I+  ++   RL T+ LN+ +++ ++           + ++ Y TQ   RPPTFV F+ 
Sbjct: 518 AAIEVEKERSRRLGTSILNQVVREAVTFKPPPRTRGGKRGRVYYCTQAAIRPPTFVFFVN 577

Query: 497 GKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGRVAERMMSD 556
                 +T  RF+ K L+ +    G PIR++ R+  K E       G  A R  E   S 
Sbjct: 578 DAKLFPETYRRFMEKQLRSNAGFPGTPIRLLWRSRRKME----KDEGKKATRPKENFASH 633

Query: 557 KRSVLA 562
           +R +++
Sbjct: 634 RRKLIS 639



 Score = 53.5 bits (127), Expect = 5e-07,   Method: Compositional matrix adjust.
 Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 6/100 (6%)

Query: 233 RVLNVNDE--GAQENSNSEDTSSPEAE---ESKLPLQLAIVGRPNVGKSTLMNTLLQEDR 287
           RVL + DE    +E   +   S P+ +   ++ LP ++AIVGRPNVGKS L N L+  ++
Sbjct: 105 RVLTIEDERSDVKETGKNSKRSKPKRKIIPDNLLP-RIAIVGRPNVGKSALFNRLVGGNK 163

Query: 288 VLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGWLQRTKQE 327
            +V  E G+TRD +  +  +      +VDT G L  +K +
Sbjct: 164 AIVVDEPGVTRDRLYGRSYWGDNEFMVVDTGGVLTVSKSQ 203


>Medtr4g065030.3 | GTPase Der protein | HC | chr4:24379001-24386603
           | 20130731
          Length = 646

 Score =  205 bits (521), Expect = 1e-52,   Method: Compositional matrix adjust.
 Identities = 167/546 (30%), Positives = 263/546 (48%), Gaps = 72/546 (13%)

Query: 50  LPISSLPTVIIIGRPNVGKSALFNRLIRRREALVYNTPEDHVTRDIREGVAKLGNLRFRV 109
           +P + LP + I+GRPNVGKSALFNRL+   +A+V + P   VTRD   G +  G+  F V
Sbjct: 133 IPDNLLPRIAIVGRPNVGKSALFNRLVGGNKAIVVDEP--GVTRDRLYGRSYWGDNEFMV 190

Query: 110 LDSAGLXXXXXXXXXILH-----------------RTAS-------ITANFFAA---SHV 142
           +D+ G+         ++                  R A+       I     AA   S V
Sbjct: 191 VDTGGVLTVSKSQTTVMEDLDISTTIGMDGIPLASREAAVARMPSMIERQAIAAVEESPV 250

Query: 143 ALFLTDARAGLHPLDQEVGKWLRKNAPQLKPIVAMNKSEXXXXXXXXXXXXGNEMCRLGF 202
            +FL D +AGL   D E+  WLRKN      I+A+NK E             +E   LG 
Sbjct: 251 IIFLVDGQAGLTAADVEIADWLRKNYSNKSIILAVNKCESPRKRIMQV----SEFWSLGL 306

Query: 203 GDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSPEAEESKLP 262
            +PI ISA +G G  DL               L++   G Q+    E+  +   EE  +P
Sbjct: 307 -EPIPISAISGTGTGDL---------------LDLVCSGLQK---VEEPQNLVEEEDYVP 347

Query: 263 LQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFEF-EGRTIYLVDTAGWL 321
             ++IVGRPNVGKS+++N L+ EDR +V P +G TRD+I T+F   +G+  +L+DTAG  
Sbjct: 348 -AISIVGRPNVGKSSILNALVGEDRTIVSPISGTTRDAIDTEFTGPDGQKFHLIDTAGIR 406

Query: 322 QRTKQEKGAAS---LSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVVIARRAVEE 378
           +RT      ++   LS+ ++ +++ R+ VVALV++A   I E+      +  IA R  +E
Sbjct: 407 KRTTVASAGSTTEALSVNRAFRAIRRSDVVALVIEAMACITEQ------DYKIAERIEKE 460

Query: 379 GRGLVVIVNKMDLLRGKASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEGRGRTAVLE 438
           G+G V++VNK D +  K    ++                      +++AL G+    ++ 
Sbjct: 461 GKGCVIVVNKWDTIPNKN---QQTALYYEQDVREKLRLLDWAPVVYSTALAGQNVDKIIV 517

Query: 439 QVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQ--AAQPKIKYFTQVKARPPTFVAFLR 496
             I+  ++   RL T+ LN+ +++ ++           + ++ Y TQ   RPPTFV F+ 
Sbjct: 518 AAIEVEKERSRRLGTSILNQVVREAVTFKPPPRTRGGKRGRVYYCTQAAIRPPTFVFFVN 577

Query: 497 GKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGRVAERMMSD 556
                 +T  RF+ K L+ +    G PIR++ R+  K E       G  A R  E   S 
Sbjct: 578 DAKLFPETYRRFMEKQLRSNAGFPGTPIRLLWRSRRKME----KDEGKKATRPKENFASH 633

Query: 557 KRSVLA 562
           +R +++
Sbjct: 634 RRKLIS 639



 Score = 53.5 bits (127), Expect = 5e-07,   Method: Compositional matrix adjust.
 Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 6/100 (6%)

Query: 233 RVLNVNDE--GAQENSNSEDTSSPEAE---ESKLPLQLAIVGRPNVGKSTLMNTLLQEDR 287
           RVL + DE    +E   +   S P+ +   ++ LP ++AIVGRPNVGKS L N L+  ++
Sbjct: 105 RVLTIEDERSDVKETGKNSKRSKPKRKIIPDNLLP-RIAIVGRPNVGKSALFNRLVGGNK 163

Query: 288 VLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGWLQRTKQE 327
            +V  E G+TRD +  +  +      +VDT G L  +K +
Sbjct: 164 AIVVDEPGVTRDRLYGRSYWGDNEFMVVDTGGVLTVSKSQ 203


>Medtr4g065030.2 | GTPase Der protein | HC | chr4:24379001-24386634
           | 20130731
          Length = 646

 Score =  205 bits (521), Expect = 1e-52,   Method: Compositional matrix adjust.
 Identities = 167/546 (30%), Positives = 263/546 (48%), Gaps = 72/546 (13%)

Query: 50  LPISSLPTVIIIGRPNVGKSALFNRLIRRREALVYNTPEDHVTRDIREGVAKLGNLRFRV 109
           +P + LP + I+GRPNVGKSALFNRL+   +A+V + P   VTRD   G +  G+  F V
Sbjct: 133 IPDNLLPRIAIVGRPNVGKSALFNRLVGGNKAIVVDEP--GVTRDRLYGRSYWGDNEFMV 190

Query: 110 LDSAGLXXXXXXXXXILH-----------------RTAS-------ITANFFAA---SHV 142
           +D+ G+         ++                  R A+       I     AA   S V
Sbjct: 191 VDTGGVLTVSKSQTTVMEDLDISTTIGMDGIPLASREAAVARMPSMIERQAIAAVEESPV 250

Query: 143 ALFLTDARAGLHPLDQEVGKWLRKNAPQLKPIVAMNKSEXXXXXXXXXXXXGNEMCRLGF 202
            +FL D +AGL   D E+  WLRKN      I+A+NK E             +E   LG 
Sbjct: 251 IIFLVDGQAGLTAADVEIADWLRKNYSNKSIILAVNKCESPRKRIMQV----SEFWSLGL 306

Query: 203 GDPIAISAETGLGMHDLYLSLKPLLEDYMLRVLNVNDEGAQENSNSEDTSSPEAEESKLP 262
            +PI ISA +G G  DL               L++   G Q+    E+  +   EE  +P
Sbjct: 307 -EPIPISAISGTGTGDL---------------LDLVCSGLQK---VEEPQNLVEEEDYVP 347

Query: 263 LQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFEF-EGRTIYLVDTAGWL 321
             ++IVGRPNVGKS+++N L+ EDR +V P +G TRD+I T+F   +G+  +L+DTAG  
Sbjct: 348 -AISIVGRPNVGKSSILNALVGEDRTIVSPISGTTRDAIDTEFTGPDGQKFHLIDTAGIR 406

Query: 322 QRTKQEKGAAS---LSIMQSRKSLLRAHVVALVLDAEEIINERRSMKHAEVVIARRAVEE 378
           +RT      ++   LS+ ++ +++ R+ VVALV++A   I E+      +  IA R  +E
Sbjct: 407 KRTTVASAGSTTEALSVNRAFRAIRRSDVVALVIEAMACITEQ------DYKIAERIEKE 460

Query: 379 GRGLVVIVNKMDLLRGKASSFEKVMXXXXXXXXXXXXXXXXXXXXFTSALEGRGRTAVLE 438
           G+G V++VNK D +  K    ++                      +++AL G+    ++ 
Sbjct: 461 GKGCVIVVNKWDTIPNKN---QQTALYYEQDVREKLRLLDWAPVVYSTALAGQNVDKIIV 517

Query: 439 QVIDTYEKWCSRLSTARLNRWMQKVMSRHSWKDQ--AAQPKIKYFTQVKARPPTFVAFLR 496
             I+  ++   RL T+ LN+ +++ ++           + ++ Y TQ   RPPTFV F+ 
Sbjct: 518 AAIEVEKERSRRLGTSILNQVVREAVTFKPPPRTRGGKRGRVYYCTQAAIRPPTFVFFVN 577

Query: 497 GKTQLADTDIRFLTKSLKEDFDLGGIPIRIMQRAVTKKEVSGNSKSGHSAGRVAERMMSD 556
                 +T  RF+ K L+ +    G PIR++ R+  K E       G  A R  E   S 
Sbjct: 578 DAKLFPETYRRFMEKQLRSNAGFPGTPIRLLWRSRRKME----KDEGKKATRPKENFASH 633

Query: 557 KRSVLA 562
           +R +++
Sbjct: 634 RRKLIS 639



 Score = 53.5 bits (127), Expect = 5e-07,   Method: Compositional matrix adjust.
 Identities = 36/100 (36%), Positives = 56/100 (56%), Gaps = 6/100 (6%)

Query: 233 RVLNVNDE--GAQENSNSEDTSSPEAE---ESKLPLQLAIVGRPNVGKSTLMNTLLQEDR 287
           RVL + DE    +E   +   S P+ +   ++ LP ++AIVGRPNVGKS L N L+  ++
Sbjct: 105 RVLTIEDERSDVKETGKNSKRSKPKRKIIPDNLLP-RIAIVGRPNVGKSALFNRLVGGNK 163

Query: 288 VLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAGWLQRTKQE 327
            +V  E G+TRD +  +  +      +VDT G L  +K +
Sbjct: 164 AIVVDEPGVTRDRLYGRSYWGDNEFMVVDTGGVLTVSKSQ 203


>Medtr3g073790.1 | tRNA modification GTPase trmE | HC |
           chr3:33306093-33311427 | 20130731
          Length = 543

 Score = 59.7 bits (143), Expect = 7e-09,   Method: Compositional matrix adjust.
 Identities = 30/57 (52%), Positives = 37/57 (64%)

Query: 263 LQLAIVGRPNVGKSTLMNTLLQEDRVLVGPEAGLTRDSIRTQFEFEGRTIYLVDTAG 319
           LQ+AIVGRPNVGKS+L+N   + +R +V   AG TRD I       G  I L+DTAG
Sbjct: 295 LQIAIVGRPNVGKSSLLNAWSKSERAIVTEIAGTTRDVIEASININGIPITLLDTAG 351