Miyakogusa Predicted Gene

Lj3g3v3755420.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3755420.1 Non Characterized Hit- tr|I1LNN6|I1LNN6_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.43804
PE,84.85,0,Elongator protein 3, MiaB family, Radical SA,Elongator
protein 3/MiaB/NifB; TRAM,TRAM domain; MTTASE,CUFF.46340.1
         (601 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g033210.1 | dimethylallyl, adenosine tRNA methylthiotransf...   985   0.0  
Medtr5g021230.1 | radical SAM methylthiotransferase, MiaB/RimO f...   134   2e-31

>Medtr4g033210.1 | dimethylallyl, adenosine tRNA
           methylthiotransferase | HC | chr4:11569877-11575926 |
           20130731
          Length = 618

 Score =  985 bits (2546), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 491/619 (79%), Positives = 520/619 (84%), Gaps = 19/619 (3%)

Query: 1   MEDIEDMLIGTTTGHPAGFRLPVAAVGVGVGTKRNQRCSPCSSA---IPGTQTIYMKTFG 57
           MEDIED+LIG  TG P GFR P+++V VGV TK+ QR S   S    IPGTQTI++KTFG
Sbjct: 1   MEDIEDLLIGVATGAPPGFRSPLSSVTVGVATKKIQRSSLLPSPSPSIPGTQTIFIKTFG 60

Query: 58  CSHNQSDSEYMAGQLSAFGYSLSDNPDEADLWLINTCTVKSPSQSAMDTIIAKGKSSNKP 117
           CSHNQSDSEYMAGQLSAFGYSLSDNPDEADLWLINTCTVKSPSQSAMDTII KGKSSNKP
Sbjct: 61  CSHNQSDSEYMAGQLSAFGYSLSDNPDEADLWLINTCTVKSPSQSAMDTIITKGKSSNKP 120

Query: 118 LVVSGCVPQGSRDLKELDGISIVGVQQIHRVVEVVEETLKGHEVRLLTRKTLPALDLPKV 177
           LVV+GCVPQGSRD+KEL+GISIVGVQQI RVVEVVEETLKGHEVRLLTRKTLPALDLPKV
Sbjct: 121 LVVAGCVPQGSRDVKELEGISIVGVQQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKV 180

Query: 178 RKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSLVGRVKSVISDGVKEIWLSSED 237
           RKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSL GRVKSV+SDGVKEIWLSSED
Sbjct: 181 RKNKFVEILPINVGCLGACTYCKTKHARGHLGSYTIDSLAGRVKSVVSDGVKEIWLSSED 240

Query: 238 TGAYGRDIGXXXXXXXXXXXXXXXXXXSTMLRIGMTNPPFILEHLKEIAEILRHPCVYSF 297
           TGAYGRDIG                  STMLRIGMTNPPFILEHLKEIAE+LRHPCVY+F
Sbjct: 241 TGAYGRDIGVNLPTLLNALVAELPADGSTMLRIGMTNPPFILEHLKEIAEVLRHPCVYTF 300

Query: 298 LHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQT 357
           LHVPVQSGSD ILSAMNREYTV EFRTVVDTLTELVPGMQIATDIICGFPGETDEDF QT
Sbjct: 301 LHVPVQSGSDAILSAMNREYTVGEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFEQT 360

Query: 358 VNLIKEYKFSQVHISQFYPRPGTPAARMKKVPSNVVKRRSRELTNVFEAFTPYSGMEGKV 417
           VNLIKEYKFSQVHISQFYPRPGTPAARMKKVPSNVVKRRSRELTNVFE+FTPYSGMEGKV
Sbjct: 361 VNLIKEYKFSQVHISQFYPRPGTPAARMKKVPSNVVKRRSRELTNVFESFTPYSGMEGKV 420

Query: 418 ERIWITDVASDGVHLVGHTKGYIQVLVIAPDYMLGTSAMVKITSVGRWSVFGEVIEMVNH 477
           ERIWITD+A+DGVHLVGHTKGYIQVLVIAPD MLG+SA+ KITSVGRWSVFGEVIE +N 
Sbjct: 421 ERIWITDIATDGVHLVGHTKGYIQVLVIAPDDMLGSSALAKITSVGRWSVFGEVIETINP 480

Query: 478 VSDDKVVNKQDPNQDM--------------QEPESCACGNDIDSCCGQSNLDKNDQSRGP 523
            SD+K +NKQ P QDM              +EPESCACGN +DSCC QS L K D SR  
Sbjct: 481 ASDNKALNKQVPTQDMSSLCCNQTKTCEISEEPESCACGN-VDSCCSQSTLVKTDNSRDT 539

Query: 524 AE-PQSQNSRNFIGWILRKRKHLHKGVESELASGSVQNQEGSMKKLDFVDKALLXXXXXX 582
              PQ+QN++N  GWILRKRK+LHK VESELASGS   QE SM+K DFVDKALL      
Sbjct: 540 VVLPQNQNNKNLFGWILRKRKNLHKRVESELASGSDLKQERSMRKWDFVDKALLGGISIS 599

Query: 583 XXXXXXXXXXLTFRVIWSQ 601
                     L F V+WSQ
Sbjct: 600 ILTIIALLVCLRFSVLWSQ 618


>Medtr5g021230.1 | radical SAM methylthiotransferase, MiaB/RimO
           family protein | HC | chr5:8174329-8167505 | 20130731
          Length = 607

 Score =  134 bits (338), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 126/493 (25%), Positives = 216/493 (43%), Gaps = 68/493 (13%)

Query: 46  PGTQTIYMKTFGCSHNQSDSEYMAGQLSAFGYS-LSDNPDEADLWLINTCTVKSPSQSAM 104
           P T  IY +T+GC  N +D E +   +   GY+ +   P+ A++  INTC ++  ++  +
Sbjct: 96  PSTGRIYHETYGCQMNVNDMEIVLSIMKNAGYNEIVTAPENAEIIFINTCAIRDNAEMKV 155

Query: 105 --------------DTIIAKGKS-SNKP--LVVSGCVPQGSRDLKELDGISIVGV----- 142
                            +A G+S S +P  +VV GC+ +  ++ K LD   +V V     
Sbjct: 156 WQRLNYFWFLKRNWKANVATGRSQSLRPPKVVVLGCMAERLKE-KILDSDKMVDVVCGPD 214

Query: 143 --QQIHRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYCK 200
             + + R++E VE   KG    L   +T   ++  ++ +N     + +  GC   C++C 
Sbjct: 215 AYRDLPRLLEEVEYGQKGINTLLSLEETYADINPVRISQNSISAFVSVMRGCNNMCSFCI 274

Query: 201 TKHARGHLGSYTIDSLVGRVKSVISDGVKEIWLSSEDTGAYGRDIGXXXXXXXXXXXXXX 260
               RG   S  ++S+V  V  +  +GVKE+ L  ++  +Y  D                
Sbjct: 275 VPFTRGRERSRPVESIVREVAELWKEGVKEVTLLGQNVNSYN-DASVVEKEAEAGSNWKL 333

Query: 261 XXXXSTMLRIGMTNPPF--ILEHLK-EIAEI-LR----HPCVY---------------SF 297
               S++ ++  T   F  +L+ L  E  E+  R    HP  Y                 
Sbjct: 334 SEGFSSISKVRTTGLRFSDLLDRLSLEFPEMRFRFTSPHPKDYPDELLYIMRERHNICKL 393

Query: 298 LHVPVQSGSDTILSAMNREYTVSEFRTVVDTLTELVPGMQIATDIICGFPGETDEDFSQT 357
           +H+P QSGS  +L  M R YT   + ++V  +  ++P + +++D ICGF GET+ED S T
Sbjct: 394 IHLPAQSGSSKMLERMRRGYTREAYLSLVQKIRSIIPDVALSSDFICGFCGETEEDHSDT 453

Query: 358 VNLIKEYKFSQVHISQFYPRPGTPAAR--MKKVPSNVVKRRSRELTNVFEAFTP--YSGM 413
           + L+KE  +   ++  +  R  T A R  +  VP    +RR  EL   F   T   Y   
Sbjct: 454 LTLVKEVGYDMAYMFAYSMREKTHAHRNYVDDVPEETKQRRLTELIETFRESTGKCYDEQ 513

Query: 414 EGKVERIWIT--DVASDGVHLVGHT-KGYIQVLVIAP-----------DYMLGTSAMVKI 459
            G  + + +   +  +    L+G + KG+  + V  P           +  +G    V+I
Sbjct: 514 VGTTQLVLVEGPNKRAPDTELIGKSDKGHRVIFVNLPISDREDVNTKRNPAVGDYVEVQI 573

Query: 460 TSVGRWSVFGEVI 472
           T   R S+FGE +
Sbjct: 574 TRSTRASLFGEAL 586