Miyakogusa Predicted Gene

Lj3g3v3690100.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3690100.1 Non Characterized Hit- tr|I3SNH2|I3SNH2_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,98.25,0,FMN-linked oxidoreductases,NULL; seg,NULL; no
description,Aldolase-type TIM barrel; Dus,tRNA-dihydro,CUFF.46215.1
         (400 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g037515.1 | tRNA-dihydrouridine synthase | HC | chr4:13808...   684   0.0  
Medtr3g030890.1 | tRNA-dihydrouridine synthase | HC | chr3:98123...   604   e-173
Medtr8g067420.1 | tRNA-dihydrouridine synthase | HC | chr8:28151...    53   4e-07

>Medtr4g037515.1 | tRNA-dihydrouridine synthase | HC |
           chr4:13808253-13802133 | 20130731
          Length = 432

 Score =  684 bits (1765), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 339/407 (83%), Positives = 359/407 (88%), Gaps = 9/407 (2%)

Query: 2   MKFSGYSLAVSFSPVGSTVI-RNNC--RFHVGXXXXXXXXXXXA-----KQQS-HDKGVL 52
           MKFSGYS++VSFSP+ S  I RN+C  R  V            +     KQQS H  GVL
Sbjct: 1   MKFSGYSISVSFSPLSSNFITRNSCSRRLPVSFSRRSRRNLCSSRVFSVKQQSYHKHGVL 60

Query: 53  PNYYLPPWFSVAPMMEWTDHHYRTLARLISKHAWLYTEMLAAETIVYQQDNLDRFLAFSP 112
              YLPPWFSVAPMM+WTD+HYRTLARLISKHAWLYTEMLAAETIV+Q+DNLDRFLA+SP
Sbjct: 61  GKQYLPPWFSVAPMMDWTDNHYRTLARLISKHAWLYTEMLAAETIVHQKDNLDRFLAYSP 120

Query: 113 DQHPIVLQIGGSNVEKLAKATELANAYGYDEINLNCGCPSPKVAGHGCFGVSLMLDPKFV 172
           +QHPIVLQIGGSN+E LAKATELANAY YDEINLNCGCPSP+VAGHG FGVSLMLDPKFV
Sbjct: 121 EQHPIVLQIGGSNIESLAKATELANAYCYDEINLNCGCPSPRVAGHGSFGVSLMLDPKFV 180

Query: 173 AEAMSAIAANTNVPVSVKCRIGVDDHDSYDELCDFIFKVSSLSPTKHFIIHSRKALLNGI 232
           AEAMSAIAANTNVPVSVKCRIGVDD DSYDELC+FI  VSSLSPTKHFIIHSRKALLNGI
Sbjct: 181 AEAMSAIAANTNVPVSVKCRIGVDDRDSYDELCEFISTVSSLSPTKHFIIHSRKALLNGI 240

Query: 233 SPAENRSIPPLKYEYFYGLLRDFPDLTFTINGGITSVDEVYAAREAGAYGVMVGRAAYNN 292
           SPAENRSIPPLKYEYFYGLLRDFPDLTFTINGGITSVDEV AAR+AGA+GVM+GRAA+ N
Sbjct: 241 SPAENRSIPPLKYEYFYGLLRDFPDLTFTINGGITSVDEVNAARDAGAHGVMIGRAAFYN 300

Query: 293 PWHILGHVDTVIYGAPSCSLTRRQVLEKYIVYGDSVLGKYGHRPTVRDIVKPLLNLFHSA 352
           PWHILGHVDT IYGAPSC LTRRQVLEKYIVYGDS LGKYGHRPTVRDIVKPLLNLFHSA
Sbjct: 301 PWHILGHVDTAIYGAPSCGLTRRQVLEKYIVYGDSELGKYGHRPTVRDIVKPLLNLFHSA 360

Query: 353 PGNGLWKRKADAAFRHCTTIKSFFEETLVAIPDSVLDSPVVEPTPGR 399
           PGNG WKR ADAAFR+CTTIKSFFEET+ AIPDSVLDSPV EP  GR
Sbjct: 361 PGNGQWKRNADAAFRNCTTIKSFFEETIDAIPDSVLDSPVSEPPLGR 407


>Medtr3g030890.1 | tRNA-dihydrouridine synthase | HC |
           chr3:9812366-9802443 | 20130731
          Length = 375

 Score =  604 bits (1557), Expect = e-173,   Method: Compositional matrix adjust.
 Identities = 285/344 (82%), Positives = 311/344 (90%)

Query: 56  YLPPWFSVAPMMEWTDHHYRTLARLISKHAWLYTEMLAAETIVYQQDNLDRFLAFSPDQH 115
           YLPPWFSVAPMM+WTD+HYRTLARLISKHAWLYTEMLAAETIVYQ+ NLDRFLA+ PDQH
Sbjct: 6   YLPPWFSVAPMMDWTDNHYRTLARLISKHAWLYTEMLAAETIVYQKGNLDRFLAYPPDQH 65

Query: 116 PIVLQIGGSNVEKLAKATELANAYGYDEINLNCGCPSPKVAGHGCFGVSLMLDPKFVAEA 175
           PIVLQIGGSN+E LAKAT+LA+AYGYDEIN NCGCPSP+VAG GCFG  LML+PKFVAEA
Sbjct: 66  PIVLQIGGSNLENLAKATKLADAYGYDEINFNCGCPSPRVAGRGCFGARLMLEPKFVAEA 125

Query: 176 MSAIAANTNVPVSVKCRIGVDDHDSYDELCDFIFKVSSLSPTKHFIIHSRKALLNGISPA 235
           +S IAANTNVPVSVKCRIGVDDHDSY+ELCDFI+KVSSLSP +HFIIHSRKALL G+SPA
Sbjct: 126 VSVIAANTNVPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPARHFIIHSRKALLKGLSPA 185

Query: 236 ENRSIPPLKYEYFYGLLRDFPDLTFTINGGITSVDEVYAAREAGAYGVMVGRAAYNNPWH 295
           ENRSIPPLKYEYFYGLLRDFPDLTFTINGGIT +DE+ AA + GA+GVM+GRAAY+NPWH
Sbjct: 186 ENRSIPPLKYEYFYGLLRDFPDLTFTINGGITCIDEINAALKTGAHGVMLGRAAYHNPWH 245

Query: 296 ILGHVDTVIYGAPSCSLTRRQVLEKYIVYGDSVLGKYGHRPTVRDIVKPLLNLFHSAPGN 355
           ILG+VD  IYG PS  LTRRQVLEKY VYGDSVL +YG RPTVRDIVKPLL  FHS PGN
Sbjct: 246 ILGNVDRAIYGVPSIGLTRRQVLEKYQVYGDSVLLQYGFRPTVRDIVKPLLGFFHSEPGN 305

Query: 356 GLWKRKADAAFRHCTTIKSFFEETLVAIPDSVLDSPVVEPTPGR 399
           GLWKRKAD+A + CTT+KSF EETLVAIPDSVLDS   +  PGR
Sbjct: 306 GLWKRKADSAVQTCTTVKSFLEETLVAIPDSVLDSSFAKSPPGR 349


>Medtr8g067420.1 | tRNA-dihydrouridine synthase | HC |
           chr8:28151579-28145915 | 20130731
          Length = 303

 Score = 53.1 bits (126), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 63/275 (22%), Positives = 113/275 (41%), Gaps = 33/275 (12%)

Query: 68  EWTDHHYRTLARLISKHAWLYTEMLAAETIVYQQDNLDRFLAFSPDQHPIVLQIGGSNVE 127
           E  DH      R I++H       L +   V +      F     +++ +V QIG SN  
Sbjct: 36  EIVDHKIIKCERRINEH-------LGSTDFVEKGTESVVFRTCDQEKNRVVFQIGTSNAV 88

Query: 128 KLAKATELANAYGYDEINLNCGCPSPKVAGHGCFGVSLMLDPKFVAEAMSAIAANTNVPV 187
           +   A +L        +++N GCP    +  G  G +L+  P+ +++ ++ +  N ++PV
Sbjct: 89  RALTAAQLV-CNDVAAVDINMGCPKA-FSLSGGMGAALLSKPELISDILTTLRRNLSLPV 146

Query: 188 SVKCRIGVDDHDSYDELCDFIFKVSSLSPTKHFIIHSRKALLNGISPAENRSIPPLKYEY 247
           + K R+    HD+  EL   I K    +      +H R  L        +R   P K+  
Sbjct: 147 TCKIRLLKSPHDTV-ELARRIEK----TGVDAIAVHGRYVL--------DRPRDPAKWNG 193

Query: 248 FYGLLRDFPDLTFTINGGITSVDEVYAAREA-GAYGVMVGRAAYNNPWHILGHVDTVIYG 306
              ++     +    NG +   D+    + A GA  VM  R A  NP  I      V Y 
Sbjct: 194 IADVVSALS-IPVIANGDVFEYDDFQRIKSATGASSVMAARGALWNP-SIFSPEGEVSY- 250

Query: 307 APSCSLTRRQVLEKYIVYGDSVLGKYGHRPTVRDI 341
                +T+++ + K I++ + +      + T+RD+
Sbjct: 251 ----EVTQKEYIRKCILWDNDI---RSTKHTLRDM 278