Miyakogusa Predicted Gene

Lj3g3v3511370.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3511370.1 tr|B9NB33|B9NB33_POPTR Predicted protein
OS=Populus trichocarpa GN=POPTRDRAFT_588111 PE=4
SV=1,86.96,0.0000000000002,C2,C2 membrane targeting protein; Protein
kinase C conserved region,C2 calcium-dependent membrane
ta,CUFF.45903.1
         (395 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g074370.1 | integral membrane single C2 domain protein | H...   494   e-140
Medtr4g108040.1 | integral membrane single C2 domain protein | H...   376   e-104
Medtr2g072180.1 | calcium-dependent lipid-binding domain protein...    57   5e-08
Medtr2g072180.2 | calcium-dependent lipid-binding domain protein...    56   6e-08
Medtr3g106660.1 | calcium-dependent lipid-binding (CaLB domain) ...    51   2e-06
Medtr4g073400.1 | calcium-dependent lipid-binding (CaLB domain) ...    50   4e-06
Medtr4g115660.1 | calcium-dependent lipid-binding (CaLB domain) ...    49   6e-06
Medtr2g030230.3 | ADP-ribosylation factor GTPase-activating prot...    49   9e-06
Medtr2g030230.2 | ADP-ribosylation factor GTPase-activating prot...    49   9e-06
Medtr2g030230.1 | ADP-ribosylation factor GTPase-activating prot...    49   9e-06

>Medtr4g074370.1 | integral membrane single C2 domain protein | HC |
           chr4:28324928-28331674 | 20130731
          Length = 690

 Score =  494 bits (1271), Expect = e-140,   Method: Compositional matrix adjust.
 Identities = 289/408 (70%), Positives = 304/408 (74%), Gaps = 20/408 (4%)

Query: 1   MLLMLSLKFXXXXXXXXXXXRDFDIDGELWVKLRLIPTAPWVGAASWAFVSLPKIKFELS 60
           MLL LSLKF           RDFDIDGELWVKLRLIPTAPWVGAASWAFVSLPKIKFELS
Sbjct: 290 MLLSLSLKFGIIPIVVPVGVRDFDIDGELWVKLRLIPTAPWVGAASWAFVSLPKIKFELS 349

Query: 61  PFRLFNLMAIPVLSMFLTKLLTVDLPKLFVRPNKIVLDFQKGKA-----VGPVPNIIKSG 115
           PFRLFNLMAIPVLSMFLTKLLTVDLP+LFVRPNKIVLDF+KGKA     VGPV ++ KSG
Sbjct: 350 PFRLFNLMAIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFEKGKAVGPVTVGPVVDVFKSG 409

Query: 116 EMPEGSKDCVGELSVTLVDARKLPYLFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGM 175
           EM EG+ D VGELSVTLVDARKLPY F+GKTDPYVILSLGDQ IRSKKNSQTTVI   G 
Sbjct: 410 EMQEGNMDSVGELSVTLVDARKLPY-FFGKTDPYVILSLGDQTIRSKKNSQTTVI---GW 465

Query: 176 PIWN-------QDFHMLVSNPRKQKLSIQVKDALGFADLTIGTGEVDLGSLKDTVPTDRI 228
             WN       QDFHMLVSNP+KQKLSIQVKDALGFADLTIGTGEVDLGSL+DTVPTDRI
Sbjct: 466 RPWNANLESAPQDFHMLVSNPKKQKLSIQVKDALGFADLTIGTGEVDLGSLQDTVPTDRI 525

Query: 229 VVLQXXXXXXXXXXXXEILLRLTYKAYVEDEEDDKTXXXXXXXXXXXXXXXXXXXXXXXN 288
           V LQ            EILLRLTYKAYVEDEEDDKT                       +
Sbjct: 526 VALQGGWVFLRKGSSGEILLRLTYKAYVEDEEDDKTEEDSIDIDVSDDELSDTEEANVTD 585

Query: 289 KKGVRDPTYEVDKESFMDVLAALIVSEEFQGIVASEAGFTKAVDNGSNIGSKLSNVSNSP 348
           K GVR+  Y  DKESFMDVLAA+IVSEEFQGIVASE GFTK +DNGSN GSK    S SP
Sbjct: 586 KMGVRESAYPTDKESFMDVLAAIIVSEEFQGIVASETGFTKGLDNGSNTGSK---ASKSP 642

Query: 349 VVNAEPI-PXXXXXXXXXXXXALIWLAVITGIALLIAVNIGGSNLFNP 395
           V NAE I P            AL WLAVIT IA+LIAVNI GS++FNP
Sbjct: 643 VANAESIPPSADNSEGSSGGSALFWLAVITSIAVLIAVNISGSSIFNP 690


>Medtr4g108040.1 | integral membrane single C2 domain protein | HC |
           chr4:44778437-44782814 | 20130731
          Length = 346

 Score =  376 bits (966), Expect = e-104,   Method: Compositional matrix adjust.
 Identities = 227/335 (67%), Positives = 243/335 (72%), Gaps = 20/335 (5%)

Query: 74  SMFLTKLLTVDLPKLFVRPNKIVLDFQKGKAVGPVP-----NIIKSGEMPEGSKDCVGEL 128
           + FLTKLLTVDLP+LFVRPNKIVLDF+KGKAVGPV      ++IKSGEM EG+ D VGEL
Sbjct: 19  TRFLTKLLTVDLPRLFVRPNKIVLDFEKGKAVGPVTVGPVVDVIKSGEMQEGNMDSVGEL 78

Query: 129 SVTLVDARKLPYLFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWN-------QD 181
           SVTLVDARKLPY F+GKTDPYVILSLGDQ IRSKKNSQTTVIG      WN       QD
Sbjct: 79  SVTLVDARKLPY-FFGKTDPYVILSLGDQTIRSKKNSQTTVIG---WRPWNANLESAPQD 134

Query: 182 FHMLVSNPRKQKLSIQVKDALGFADLTIGTGEVDLGSLKDTVPTDRIVVLQXXXXXXXXX 241
           FHMLVSNP+KQKLSIQVKDALGFADLTIGTGEVDLGSL+DTVPTDRIV LQ         
Sbjct: 135 FHMLVSNPKKQKLSIQVKDALGFADLTIGTGEVDLGSLQDTVPTDRIVALQGGWGFLRKG 194

Query: 242 XXXEILLRLTYKAYVEDEEDDKTXXXXXXXXXXXXXXXXXXXXXXXNKKGVRDPTYEVDK 301
              EILLRLTYKAYVEDEEDDKT                       +K GVR+  Y  DK
Sbjct: 195 LSGEILLRLTYKAYVEDEEDDKTEEDSIDIDVSDDELSDTEEANVTDKTGVRESAYPTDK 254

Query: 302 ESFMDVLAALIVSEEFQGIVASEAGFTKAVDNGSNIGSKLSNVSNSPVVNAEPI-PXXXX 360
           ESFMDVLAA+IVSEEFQGIVASE GFTK +DNGSN GSK    S SPV NAE I P    
Sbjct: 255 ESFMDVLAAIIVSEEFQGIVASETGFTKGLDNGSNTGSK---ASKSPVANAESIPPSADN 311

Query: 361 XXXXXXXXALIWLAVITGIALLIAVNIGGSNLFNP 395
                   AL WLAVIT IA+LIAVNI GS++FNP
Sbjct: 312 SEGSSGGSALFWLAVITSIAVLIAVNISGSSIFNP 346


>Medtr2g072180.1 | calcium-dependent lipid-binding domain protein |
           HC | chr2:30311117-30303509 | 20130731
          Length = 758

 Score = 56.6 bits (135), Expect = 5e-08,   Method: Compositional matrix adjust.
 Identities = 50/212 (23%), Positives = 99/212 (46%), Gaps = 16/212 (7%)

Query: 25  IDGELWVKLRLIPTAPWVGAASWAFVSLPKIKFELSPF--RLFNLMAIPVLSMFLTKLLT 82
           ++G++ V ++ +   P++G     FV  P  +  + P      ++  +P ++ +L KLL+
Sbjct: 188 VEGKVLVGVKFLKEWPFLGRIRVCFVEPPYFQMTVKPIFTHGLDVTELPGIAGWLDKLLS 247

Query: 83  VDLPKLFVRPNKIVLDFQKGKAVGPVPNIIKSGEMPEGSKDCVGELSVTLVDARKL-PYL 141
           +   +  V PN +V+D +K  +    P   K  E     K+ V    + +V+A ++ P  
Sbjct: 248 IAFEQTLVEPNMLVVDVEKFVSPDQEP-WFKVDE-----KEPVAYAKIEIVEAAEMKPSD 301

Query: 142 FYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHM-LVSNPRKQKLSIQVKD 200
             G  DPY+   LG    R+K   +T        P W ++F + +++      L+I+V+D
Sbjct: 302 LNGLADPYLKGQLGGYRFRTKVQKKTL------SPKWQEEFRIPIITWDCNNVLAIEVRD 355

Query: 201 ALGFADLTIGTGEVDLGSLKDTVPTDRIVVLQ 232
              F D  +G   V++   +D    D+ + L+
Sbjct: 356 KDHFYDDILGECSVNINDFRDGQKYDKWLPLE 387


>Medtr2g072180.2 | calcium-dependent lipid-binding domain protein |
           HC | chr2:30310420-30303509 | 20130731
          Length = 668

 Score = 56.2 bits (134), Expect = 6e-08,   Method: Compositional matrix adjust.
 Identities = 50/212 (23%), Positives = 99/212 (46%), Gaps = 16/212 (7%)

Query: 25  IDGELWVKLRLIPTAPWVGAASWAFVSLPKIKFELSPF--RLFNLMAIPVLSMFLTKLLT 82
           ++G++ V ++ +   P++G     FV  P  +  + P      ++  +P ++ +L KLL+
Sbjct: 98  VEGKVLVGVKFLKEWPFLGRIRVCFVEPPYFQMTVKPIFTHGLDVTELPGIAGWLDKLLS 157

Query: 83  VDLPKLFVRPNKIVLDFQKGKAVGPVPNIIKSGEMPEGSKDCVGELSVTLVDARKL-PYL 141
           +   +  V PN +V+D +K  +    P   K  E     K+ V    + +V+A ++ P  
Sbjct: 158 IAFEQTLVEPNMLVVDVEKFVSPDQEP-WFKVDE-----KEPVAYAKIEIVEAAEMKPSD 211

Query: 142 FYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHM-LVSNPRKQKLSIQVKD 200
             G  DPY+   LG    R+K   +T        P W ++F + +++      L+I+V+D
Sbjct: 212 LNGLADPYLKGQLGGYRFRTKVQKKTL------SPKWQEEFRIPIITWDCNNVLAIEVRD 265

Query: 201 ALGFADLTIGTGEVDLGSLKDTVPTDRIVVLQ 232
              F D  +G   V++   +D    D+ + L+
Sbjct: 266 KDHFYDDILGECSVNINDFRDGQKYDKWLPLE 297


>Medtr3g106660.1 | calcium-dependent lipid-binding (CaLB domain)
           family protein | HC | chr3:49295362-49289363 | 20130731
          Length = 565

 Score = 51.2 bits (121), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 30/86 (34%), Positives = 47/86 (54%), Gaps = 5/86 (5%)

Query: 126 GELSVTLVDARKLPYL-FYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHM 184
           G LSVT++ A  LP + F GK DP+V+L+L     +S+K  +T V+     P+WNQ F  
Sbjct: 440 GVLSVTVISAEDLPIVDFMGKADPFVVLALK----KSEKKQKTRVVNETLNPVWNQTFDF 495

Query: 185 LVSNPRKQKLSIQVKDALGFADLTIG 210
           +V +     L +++ D   F    +G
Sbjct: 496 VVEDGLHDMLIVELWDHDTFGKEKMG 521


>Medtr4g073400.1 | calcium-dependent lipid-binding (CaLB domain)
           family protein | HC | chr4:27791390-27799317 | 20130731
          Length = 537

 Score = 50.1 bits (118), Expect = 4e-06,   Method: Compositional matrix adjust.
 Identities = 45/167 (26%), Positives = 76/167 (45%), Gaps = 20/167 (11%)

Query: 35  LIPTAPWVGAASWAFVSLPKIKFELSPFRLFNLMAIPVLSMFLTKLLTVDLPKLFVRPNK 94
           L+P+ P       A +  P + F L      +LM+IP +   + +L+   +  +++ P  
Sbjct: 185 LVPSFPCFANIYVALMEKPHVDFGLKLLGA-DLMSIPGVYRIVQELIKDQVANMYLWPKN 243

Query: 95  IVLDFQKGKAVGPVPNIIKSGEMPEGSKDCVGELSVTLVDARKLPYL-FYGKTDPYVILS 153
           +                ++  +M +  +  VG L V ++ A KL      G +DPYV L 
Sbjct: 244 LE---------------VQILDMAKAMRRPVGILHVKVLHAMKLKKKDLLGASDPYVKLK 288

Query: 154 LGDQIIRSKKNSQTTVIGPPGMPIWNQDFHMLVSNPRKQKLSIQVKD 200
           L D  + SKK   TTV      P WN++F+++V +P  Q L + V D
Sbjct: 289 LTDDKMPSKK---TTVKHKNLNPEWNEEFNLVVKDPETQVLQLNVYD 332


>Medtr4g115660.1 | calcium-dependent lipid-binding (CaLB domain)
           family protein | HC | chr4:47788220-47795661 | 20130731
          Length = 566

 Score = 49.3 bits (116), Expect = 6e-06,   Method: Compositional matrix adjust.
 Identities = 29/86 (33%), Positives = 48/86 (55%), Gaps = 5/86 (5%)

Query: 126 GELSVTLVDARKLPYL-FYGKTDPYVILSLGDQIIRSKKNSQTTVIGPPGMPIWNQDFHM 184
           G LSVT++ A  LP + F GK+DP+V+L+L     +++  ++T V+     P+WNQ F  
Sbjct: 441 GVLSVTVISAEDLPAVDFMGKSDPFVVLTLK----KAETKNKTRVVNNSLNPVWNQTFDF 496

Query: 185 LVSNPRKQKLSIQVKDALGFADLTIG 210
           +V +     L ++V D   F    +G
Sbjct: 497 VVEDGLHDMLLVEVYDHDTFGKDYMG 522


>Medtr2g030230.3 | ADP-ribosylation factor GTPase-activating protein
           AGD10 | HC | chr2:11341357-11334237 | 20130731
          Length = 329

 Score = 48.9 bits (115), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 35/107 (32%), Positives = 55/107 (51%), Gaps = 8/107 (7%)

Query: 114 SGEMPEGSKDCVGELSVTLVDARKLPYLFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPP 173
           + E  EG  + +G L V ++    L       +DPYV+L+LG Q +      QT+V+   
Sbjct: 161 NSERMEGMVEFIGMLKVKVIKGTDLAVRDIKSSDPYVVLNLGTQTV------QTSVMRSN 214

Query: 174 GMPIWNQDFHMLVSNPRKQKLSIQVKDALGF-ADLTIGTGEVDLGSL 219
             P+WN++ HML       +L ++V D   F AD  +G  ++DL SL
Sbjct: 215 LNPVWNEE-HMLSVPEHYGQLKLKVFDHDTFSADDIMGEADIDLQSL 260


>Medtr2g030230.2 | ADP-ribosylation factor GTPase-activating protein
           AGD10 | HC | chr2:11341357-11334237 | 20130731
          Length = 329

 Score = 48.9 bits (115), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 35/107 (32%), Positives = 55/107 (51%), Gaps = 8/107 (7%)

Query: 114 SGEMPEGSKDCVGELSVTLVDARKLPYLFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPP 173
           + E  EG  + +G L V ++    L       +DPYV+L+LG Q +      QT+V+   
Sbjct: 161 NSERMEGMVEFIGMLKVKVIKGTDLAVRDIKSSDPYVVLNLGTQTV------QTSVMRSN 214

Query: 174 GMPIWNQDFHMLVSNPRKQKLSIQVKDALGF-ADLTIGTGEVDLGSL 219
             P+WN++ HML       +L ++V D   F AD  +G  ++DL SL
Sbjct: 215 LNPVWNEE-HMLSVPEHYGQLKLKVFDHDTFSADDIMGEADIDLQSL 260


>Medtr2g030230.1 | ADP-ribosylation factor GTPase-activating protein
           AGD10 | HC | chr2:11341357-11334237 | 20130731
          Length = 329

 Score = 48.9 bits (115), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 35/107 (32%), Positives = 55/107 (51%), Gaps = 8/107 (7%)

Query: 114 SGEMPEGSKDCVGELSVTLVDARKLPYLFYGKTDPYVILSLGDQIIRSKKNSQTTVIGPP 173
           + E  EG  + +G L V ++    L       +DPYV+L+LG Q +      QT+V+   
Sbjct: 161 NSERMEGMVEFIGMLKVKVIKGTDLAVRDIKSSDPYVVLNLGTQTV------QTSVMRSN 214

Query: 174 GMPIWNQDFHMLVSNPRKQKLSIQVKDALGF-ADLTIGTGEVDLGSL 219
             P+WN++ HML       +L ++V D   F AD  +G  ++DL SL
Sbjct: 215 LNPVWNEE-HMLSVPEHYGQLKLKVFDHDTFSADDIMGEADIDLQSL 260