Miyakogusa Predicted Gene

Lj3g3v3430170.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3430170.1 Non Characterized Hit- tr|I1JVT1|I1JVT1_SOYBN
Uncharacterized protein OS=Glycine max PE=4 SV=1,83.86,0,GB DEF:
HYPOTHETICAL PROTEIN C875ERIPDS,NULL; SPHINGOSINE KINASE,NULL;
DAGK,Diacylglycerol kinase, c,CUFF.45807.1
         (405 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g008260.1 | ceramide kinase-like protein | HC | chr3:13890...   670   0.0  
Medtr3g008260.2 | ceramide kinase-like protein | HC | chr3:13890...   668   0.0  
Medtr2g011820.1 | D-erythro-sphingosine kinase/diacylglycerol ki...    67   3e-11
Medtr6g004070.1 | sphingoid long-chain bases kinase-like protein...    60   5e-09
Medtr6g004070.2 | sphingoid long-chain bases kinase-like protein...    60   5e-09

>Medtr3g008260.1 | ceramide kinase-like protein | HC |
           chr3:1389057-1396955 | 20130731
          Length = 618

 Score =  670 bits (1728), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 323/406 (79%), Positives = 360/406 (88%), Gaps = 3/406 (0%)

Query: 1   MLSITNMELNSYXXXXXXXXXXFFNEILNGFLSPRLKAPYPPTPSDFVHLAKDSGDSLVF 60
           M S+TN ELNSY          FFNEILNGFLSPRLKAPYPPTP DFVHLAKD GDSLV 
Sbjct: 215 MSSLTNKELNSYDGAIAVGGDGFFNEILNGFLSPRLKAPYPPTPPDFVHLAKDKGDSLVV 274

Query: 61  DKDEIVEEASSQSEDQFPLISNPKQSGCTMSNSIGDSEDKALEFPVPNERFRFGIIPAGS 120
           D++E+ EE SSQSEDQFPLIS+  QSG  +SNS   SEDKA EFPVPNE FRFGIIPAGS
Sbjct: 275 DENEVFEETSSQSEDQFPLISSANQSGLRISNS--SSEDKAPEFPVPNEWFRFGIIPAGS 332

Query: 121 TDAIVICTTGTRDPVTSALHIVLGKRVDLDIAQVVRWKTTPGSEVEPNVRYAASFSGYGF 180
           TDAIVICTTG RDP+TSALHIVLGKRV LDIAQVVRWK TP SEVEP VRYAASFSGYGF
Sbjct: 333 TDAIVICTTGARDPITSALHIVLGKRVHLDIAQVVRWKKTPRSEVEPLVRYAASFSGYGF 392

Query: 181 YGDVISESEKYRWMGPKRYDYAGTMVFLKHRSYEAEIAYLDVESDETKLTT-KRNRQGSL 239
           YGDVI ESEKYRWMGPKRYDYAGT+VFL+HRSYEAEI++LD ES+ET  TT KR+R+ SL
Sbjct: 393 YGDVIKESEKYRWMGPKRYDYAGTVVFLRHRSYEAEISFLDDESEETDSTTSKRSRESSL 452

Query: 240 LRAVRTPQISERSICRINCKVCSEKPNHASSGTRSLTPHLHSEEGRWVRSKGRFLSVGAA 299
           LR +++P+ SER ICRINCKVC+EKPN+A++GT SLTPHL+SE+ RWV+SKGRF+SVGAA
Sbjct: 453 LRGLKSPRRSERCICRINCKVCNEKPNNAAAGTCSLTPHLNSEKRRWVKSKGRFISVGAA 512

Query: 300 VISCRNEKAPDGLVADAHLSDGFLHLILIRDCPHPSYLWHLTQLTRRGGSPLNFKFVEHH 359
           VIS RNEKAPDGLVADAHLSDGFLHLI+I++CPH SYLWHLTQLT+RGGSPLNFKFVEH 
Sbjct: 513 VISNRNEKAPDGLVADAHLSDGFLHLIMIKECPHASYLWHLTQLTKRGGSPLNFKFVEHC 572

Query: 360 KTPAFTFTSLGSQSVWNLDGEIFQAHQLSAQVFRGLICLFATGPEV 405
           KTPAFTFTS G++SVWN+DGE+FQAHQLSAQVFRGL+C+FATGPE+
Sbjct: 573 KTPAFTFTSSGNESVWNVDGEMFQAHQLSAQVFRGLVCMFATGPEI 618


>Medtr3g008260.2 | ceramide kinase-like protein | HC |
           chr3:1389057-1396955 | 20130731
          Length = 504

 Score =  668 bits (1724), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 323/406 (79%), Positives = 360/406 (88%), Gaps = 3/406 (0%)

Query: 1   MLSITNMELNSYXXXXXXXXXXFFNEILNGFLSPRLKAPYPPTPSDFVHLAKDSGDSLVF 60
           M S+TN ELNSY          FFNEILNGFLSPRLKAPYPPTP DFVHLAKD GDSLV 
Sbjct: 101 MSSLTNKELNSYDGAIAVGGDGFFNEILNGFLSPRLKAPYPPTPPDFVHLAKDKGDSLVV 160

Query: 61  DKDEIVEEASSQSEDQFPLISNPKQSGCTMSNSIGDSEDKALEFPVPNERFRFGIIPAGS 120
           D++E+ EE SSQSEDQFPLIS+  QSG  +SNS   SEDKA EFPVPNE FRFGIIPAGS
Sbjct: 161 DENEVFEETSSQSEDQFPLISSANQSGLRISNS--SSEDKAPEFPVPNEWFRFGIIPAGS 218

Query: 121 TDAIVICTTGTRDPVTSALHIVLGKRVDLDIAQVVRWKTTPGSEVEPNVRYAASFSGYGF 180
           TDAIVICTTG RDP+TSALHIVLGKRV LDIAQVVRWK TP SEVEP VRYAASFSGYGF
Sbjct: 219 TDAIVICTTGARDPITSALHIVLGKRVHLDIAQVVRWKKTPRSEVEPLVRYAASFSGYGF 278

Query: 181 YGDVISESEKYRWMGPKRYDYAGTMVFLKHRSYEAEIAYLDVESDETKLTT-KRNRQGSL 239
           YGDVI ESEKYRWMGPKRYDYAGT+VFL+HRSYEAEI++LD ES+ET  TT KR+R+ SL
Sbjct: 279 YGDVIKESEKYRWMGPKRYDYAGTVVFLRHRSYEAEISFLDDESEETDSTTSKRSRESSL 338

Query: 240 LRAVRTPQISERSICRINCKVCSEKPNHASSGTRSLTPHLHSEEGRWVRSKGRFLSVGAA 299
           LR +++P+ SER ICRINCKVC+EKPN+A++GT SLTPHL+SE+ RWV+SKGRF+SVGAA
Sbjct: 339 LRGLKSPRRSERCICRINCKVCNEKPNNAAAGTCSLTPHLNSEKRRWVKSKGRFISVGAA 398

Query: 300 VISCRNEKAPDGLVADAHLSDGFLHLILIRDCPHPSYLWHLTQLTRRGGSPLNFKFVEHH 359
           VIS RNEKAPDGLVADAHLSDGFLHLI+I++CPH SYLWHLTQLT+RGGSPLNFKFVEH 
Sbjct: 399 VISNRNEKAPDGLVADAHLSDGFLHLIMIKECPHASYLWHLTQLTKRGGSPLNFKFVEHC 458

Query: 360 KTPAFTFTSLGSQSVWNLDGEIFQAHQLSAQVFRGLICLFATGPEV 405
           KTPAFTFTS G++SVWN+DGE+FQAHQLSAQVFRGL+C+FATGPE+
Sbjct: 459 KTPAFTFTSSGNESVWNVDGEMFQAHQLSAQVFRGLVCMFATGPEI 504


>Medtr2g011820.1 | D-erythro-sphingosine kinase/diacylglycerol
           kinase | HC | chr2:2972958-2979099 | 20130731
          Length = 484

 Score = 67.0 bits (162), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 64/286 (22%), Positives = 116/286 (40%), Gaps = 49/286 (17%)

Query: 113 FGIIPAGSTDAI---VICTTGTRDPVTSA-LHIVLGKRVDLDIAQVVRWKTTPGSEVEPN 168
            GI+PAG+ + +   ++ + G    + +A L I+ G +  LD+A + + +T         
Sbjct: 197 LGIVPAGTGNGMAKSLLDSVGDPCAIANAVLAIIRGHKRQLDVATITQGETR-------- 248

Query: 169 VRYAASFSGYGFYGDVISESEKYRWMGPKRYDYAGTMVFLKHRSYEAEIAYL-----DVE 223
             ++     +G   D+  ESEKYRWMG  R D+         R Y   ++++     +  
Sbjct: 249 -FFSILMLAWGLIADIDIESEKYRWMGSARLDFYAVCRLFNLRLYNGSVSFVPAPGFESY 307

Query: 224 SDETKLTTKRNRQGSLLRAVRTPQISERSICRINCKVCSEKPNHASSGTRSLTPHLHSEE 283
            + T    K   +G+         ++ +++C                      P +  E 
Sbjct: 308 GESTSYPGKSTIRGNSSDPSEGEHVNLQTLCYQG-------------------PEIDLEN 348

Query: 284 GRWVRSKGRFLSVGAAVISCRNEKAPDGLVA-DAHLSDGFLHLILIRDCPHPSYLWHLTQ 342
             W    G F+SV    +      A D + A DA  SDG+L LI+  +CP    L  +++
Sbjct: 349 MNWRVINGPFISVWVHNVPW---GAEDTMAAPDAKFSDGYLDLIITMNCPKLHLLSMMSE 405

Query: 343 LTRRGGSPLNFKFVEHHKTPAFTF------TSLGSQSVWNLDGEIF 382
           L +  G  +   +V + K  AF+         L  + + + DGE+ 
Sbjct: 406 LNK--GGHVKSPYVTYLKVKAFSLEPGTRTKDLEKEGIIDSDGEVL 449


>Medtr6g004070.1 | sphingoid long-chain bases kinase-like protein |
           HC | chr6:102237-110525 | 20130731
          Length = 777

 Score = 59.7 bits (143), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 41/118 (34%), Positives = 60/118 (50%), Gaps = 10/118 (8%)

Query: 118 AGSTDAIVICTTGTRDPVTSALHIVLGKRVDLDIAQVVRWKTTPGSEVEPN-VRYAASFS 176
           AGS +++V    G RDPV++A+ IV G     D+   V W       ++ N + +  + S
Sbjct: 365 AGSDNSLVWTVLGVRDPVSAAMAIVKGGLTATDVF-AVEW-------IQNNKIHFGLTVS 416

Query: 177 GYGFYGDVISESEKY-RWMGPKRYDYAGTMVFLKHRSYEAEIAYLDVESDETKLTTKR 233
            YGF  DV+  SEKY +  GP RY  AG + FL    Y  E+ YL     E + + +R
Sbjct: 417 YYGFVSDVLELSEKYQKRFGPLRYFVAGFLKFLCLPRYSYEVEYLPASKPEKEGSGER 474


>Medtr6g004070.2 | sphingoid long-chain bases kinase-like protein |
           HC | chr6:102250-110482 | 20130731
          Length = 739

 Score = 59.7 bits (143), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 41/118 (34%), Positives = 60/118 (50%), Gaps = 10/118 (8%)

Query: 118 AGSTDAIVICTTGTRDPVTSALHIVLGKRVDLDIAQVVRWKTTPGSEVEPN-VRYAASFS 176
           AGS +++V    G RDPV++A+ IV G     D+   V W       ++ N + +  + S
Sbjct: 365 AGSDNSLVWTVLGVRDPVSAAMAIVKGGLTATDVF-AVEW-------IQNNKIHFGLTVS 416

Query: 177 GYGFYGDVISESEKY-RWMGPKRYDYAGTMVFLKHRSYEAEIAYLDVESDETKLTTKR 233
            YGF  DV+  SEKY +  GP RY  AG + FL    Y  E+ YL     E + + +R
Sbjct: 417 YYGFVSDVLELSEKYQKRFGPLRYFVAGFLKFLCLPRYSYEVEYLPASKPEKEGSGER 474