Miyakogusa Predicted Gene
- Lj3g3v3336280.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj3g3v3336280.1 Non Characterized Hit- tr|I3SWP1|I3SWP1_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,100,0,SUBFAMILY
NOT NAMED,NULL; EPIDIDYMAL MEMBRANE PROTEIN E9-RELATED,Protein of
unknown function DUF716,,CUFF.45596.1
(293 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr4g068340.1 | T1.3 protein | HC | chr4:25626826-25625439 | 2... 425 e-119
Medtr2g021690.1 | T1.3 protein | HC | chr2:7380379-7382066 | 201... 121 8e-28
Medtr8g071150.1 | transmembrane 45B-like protein | HC | chr8:301... 107 1e-23
Medtr8g071150.2 | transmembrane 45B-like protein | HC | chr8:301... 107 1e-23
Medtr1g112160.1 | T1.3 protein | HC | chr1:50718071-50718826 | 2... 103 2e-22
Medtr5g027180.1 | transmembrane 45B-like protein | HC | chr5:112... 97 1e-20
Medtr5g027180.2 | transmembrane 45B-like protein | HC | chr5:112... 97 1e-20
Medtr8g071140.1 | transmembrane 45B-like protein | LC | chr8:301... 96 4e-20
Medtr1g112150.1 | T1.3 protein | LC | chr1:50712643-50713536 | 2... 91 1e-18
>Medtr4g068340.1 | T1.3 protein | HC | chr4:25626826-25625439 |
20130731
Length = 293
Score = 425 bits (1092), Expect = e-119, Method: Compositional matrix adjust.
Identities = 206/293 (70%), Positives = 229/293 (78%)
Query: 1 MSLFVEHLVQXXXXXXXXXWHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI 60
M LF+ HLVQ WH INTIKSYLVKGP+NFT+RFW+ FNTP + KHLELVSI
Sbjct: 1 MDLFIAHLVQGLALTFLGLWHIINTIKSYLVKGPSNFTIRFWYHFNTPHFKFKHLELVSI 60
Query: 61 LCFSILAIFTQILDFPNFHYAFKLHNFEHATMFIHLALFAGFSLSIELTDSLDIFSGFVG 120
L FSILAI QILDFP+FHYAFKL NFEHATMFIHLALFAGFSL ELTDSL++FSGFV
Sbjct: 61 LSFSILAILMQILDFPHFHYAFKLDNFEHATMFIHLALFAGFSLLTELTDSLELFSGFVA 120
Query: 121 ILASSMFSQELFLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAIFPQSFNAALVLS 180
IL SS+FSQELFLLHFHSTDHVG EGHYHW ++AAA+FP SFNAA+VLS
Sbjct: 121 ILVSSVFSQELFLLHFHSTDHVGPEGHYHWLLQLIVFVSLIASLAAAVFPNSFNAAIVLS 180
Query: 181 ISIIFQGCWFINMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSKEADFRARGLANL 240
IS+IFQGCWFINMGFML+IP +PEGCVMN A+ + S I GAV CGSKEADFRA+GLANL
Sbjct: 181 ISLIFQGCWFINMGFMLYIPTFVPEGCVMNLARESASEIHGAVTCGSKEADFRAKGLANL 240
Query: 241 QFSWXXXXXXXXXXXXCLKLARKCTILDRLEYERIQNKGTDSTMANEGFKQGK 293
QFSW CLKLA+K TI+DRLEYERIQ+K DST+ NEGFK K
Sbjct: 241 QFSWILSAIMIFAGIVCLKLAKKFTIVDRLEYERIQSKVADSTVVNEGFKPAK 293
>Medtr2g021690.1 | T1.3 protein | HC | chr2:7380379-7382066 |
20130731
Length = 318
Score = 121 bits (304), Expect = 8e-28, Method: Compositional matrix adjust.
Identities = 85/255 (33%), Positives = 120/255 (47%), Gaps = 24/255 (9%)
Query: 1 MSLFVEHLVQXXXXXXXXXWHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI 60
M V H+ WH N IK + +K P ++ WF P ++ K+LEL I
Sbjct: 1 MGTLVGHVAPGFAFLILGLWHLFNHIKLH-IKNPNSYISPTWF----PTSKFKYLELYLI 55
Query: 61 LCFSILAIFTQILDFPNFHYAFK---------LHNFEHATMFIHLALFAGFSLSIELTDS 111
+ + +I ++ P H F LHNFEHA I +A FA + SI L D
Sbjct: 56 MLATTASISMELFIGPARHQPFDTDGTIPSYHLHNFEHA--LISMAFFAYAASSIIL-DK 112
Query: 112 LDIFS--GFVGILASSMFSQELFLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAIF 169
++ + LAS F Q+L L H HS DH+G EG YH +
Sbjct: 113 IENQAKLALTQFLASMAFFQQLLLFHLHSADHMGPEGQYHLLLQVLVFVSLSTTLLGIPM 172
Query: 170 PQSFNAALVLSISIIFQGCWFINMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSKE 229
+SF + V S+SI FQG W + MG+MLW P+ I +GC MN+ + ++ V C +E
Sbjct: 173 QKSFLVSFVRSLSIFFQGLWLMVMGYMLWTPSLIAKGCFMNSEEGHK-----VVRCSDEE 227
Query: 230 ADFRARGLANLQFSW 244
+ RA L N+ FSW
Sbjct: 228 SLHRAVSLVNILFSW 242
>Medtr8g071150.1 | transmembrane 45B-like protein | HC |
chr8:30189066-30192021 | 20130731
Length = 269
Score = 107 bits (267), Expect = 1e-23, Method: Compositional matrix adjust.
Identities = 81/249 (32%), Positives = 114/249 (45%), Gaps = 20/249 (8%)
Query: 1 MSLFVEHLVQXXXXXXXXXWHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI 60
M F+ H++ WH ++ Y V P F VR W RLK+LEL I
Sbjct: 1 MGSFIGHILPGTLFLLVGVWHIWCSVVRY-VSSPKTFQVRAWNPVPGFEGRLKYLELYVI 59
Query: 61 -------LCFSILAIFTQILDF-PNFHYAFKLHNFEHATMFIHLALFAGFSLSIELTDSL 112
LC +L + TQ+ F + L+NFEH+ M + +F SL E T +
Sbjct: 60 SIGAFIDLCVELL-VATQLKFFVGGVLNSTYLNNFEHSGMLLMFLIFGVVSLLSEKTRYI 118
Query: 113 DIFSGFVGILASSMFSQELFLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAIFPQS 172
+ G + ++A++ F+ E L +FHS H GLEG+YH ++A A+ P
Sbjct: 119 PLPEGALCLIAATAFTAEYLLFNFHSATHKGLEGYYHVLLAFLIGLCILSSIAGAVLPTC 178
Query: 173 FNAALVLSISIIFQGCWFINMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSKEADF 232
F L I I QG WF F+L+ P +P GC M K N V C SKE++
Sbjct: 179 FPVDLSKGIGITLQGIWFYQTAFVLFGPM-LPSGCSM---KDN------VVTCHSKESEV 228
Query: 233 RARGLANLQ 241
R LAN+Q
Sbjct: 229 RGELLANVQ 237
>Medtr8g071150.2 | transmembrane 45B-like protein | HC |
chr8:30189649-30192021 | 20130731
Length = 269
Score = 107 bits (267), Expect = 1e-23, Method: Compositional matrix adjust.
Identities = 81/249 (32%), Positives = 114/249 (45%), Gaps = 20/249 (8%)
Query: 1 MSLFVEHLVQXXXXXXXXXWHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI 60
M F+ H++ WH ++ Y V P F VR W RLK+LEL I
Sbjct: 1 MGSFIGHILPGTLFLLVGVWHIWCSVVRY-VSSPKTFQVRAWNPVPGFEGRLKYLELYVI 59
Query: 61 -------LCFSILAIFTQILDF-PNFHYAFKLHNFEHATMFIHLALFAGFSLSIELTDSL 112
LC +L + TQ+ F + L+NFEH+ M + +F SL E T +
Sbjct: 60 SIGAFIDLCVELL-VATQLKFFVGGVLNSTYLNNFEHSGMLLMFLIFGVVSLLSEKTRYI 118
Query: 113 DIFSGFVGILASSMFSQELFLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAIFPQS 172
+ G + ++A++ F+ E L +FHS H GLEG+YH ++A A+ P
Sbjct: 119 PLPEGALCLIAATAFTAEYLLFNFHSATHKGLEGYYHVLLAFLIGLCILSSIAGAVLPTC 178
Query: 173 FNAALVLSISIIFQGCWFINMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSKEADF 232
F L I I QG WF F+L+ P +P GC M K N V C SKE++
Sbjct: 179 FPVDLSKGIGITLQGIWFYQTAFVLFGPM-LPSGCSM---KDN------VVTCHSKESEV 228
Query: 233 RARGLANLQ 241
R LAN+Q
Sbjct: 229 RGELLANVQ 237
>Medtr1g112160.1 | T1.3 protein | HC | chr1:50718071-50718826 |
20130731
Length = 251
Score = 103 bits (257), Expect = 2e-22, Method: Compositional matrix adjust.
Identities = 56/162 (34%), Positives = 84/162 (51%), Gaps = 6/162 (3%)
Query: 84 LHNFEHATMFIHLALFAGFSLSIEL-TDSLDIFSGFVGILASSMFSQELFLLHFHSTDHV 142
LHNFEH++M + ++A F++ ++ + S + +LA+ F+Q+ L+H HS DH+
Sbjct: 34 LHNFEHSSMALSFLVYATFAMILDRKSTSTKSQNEITHLLAAIAFAQQFLLIHLHSRDHM 93
Query: 143 GLEGHYHWXXXXXXXXXXXXAMAAAIFPQSFNAALVLSISIIFQGCWFINMGFMLWIPAC 202
G+EG YH+ + +P SF + V S+SI FQG W + MG ML+ P
Sbjct: 94 GIEGQYHYLLQVLISICFVTTLMGIGYPMSFLVSFVRSVSITFQGLWLMFMGLMLFTPGF 153
Query: 203 IPEGCVMNTAKPNQSRILGAVACGSKEADFRARGLANLQFSW 244
P+GC V C ++A RA L NLQFSW
Sbjct: 154 EPKGCFTKLEGDQY-----VVKCSDEKALHRAVSLVNLQFSW 190
>Medtr5g027180.1 | transmembrane 45B-like protein | HC |
chr5:11271754-11267763 | 20130731
Length = 278
Score = 97.4 bits (241), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 76/253 (30%), Positives = 110/253 (43%), Gaps = 28/253 (11%)
Query: 1 MSLFVEHLVQXXXXXXXXXWHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI 60
M F H + WH +I Y V+ P F VR W RLK+LEL +
Sbjct: 1 MGSFKGHALPGTLFFVVGVWHIWASIVRY-VRNPKMFRVRAWNPVPGFDGRLKYLELYVV 59
Query: 61 -------LCFSIL-----AIFTQILDFPNFHYAFKLHNFEHATMFIHLALFAGFSLSIEL 108
LC L IF + P+ +++FEH+ M + ++ +L E
Sbjct: 60 AIGAFIDLCVEFLYSTHLKIFVGGVLNPS-----HMNDFEHSGMLLMFFIYGVVALLSEK 114
Query: 109 TDSLDIFSGFVGILASSMFSQELFLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAI 168
T L + G + LA+ FS E L FHST H GLEG+YH ++A A+
Sbjct: 115 TRFLPLQEGVLCFLAAMAFSAEYLLFSFHSTSHKGLEGYYHTLLVFQIGLCILSSIAGAL 174
Query: 169 FPQSFNAALVLSISIIFQGCWFINMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSK 228
P +F A L I++ QG WF F+L+ P +P+GC + K + C S
Sbjct: 175 LPNNFPADLSNGIALALQGIWFYQTAFVLYGP-MLPDGCKIKDNK---------IMCNSV 224
Query: 229 EADFRARGLANLQ 241
+++ R LAN Q
Sbjct: 225 DSEIRGELLANFQ 237
>Medtr5g027180.2 | transmembrane 45B-like protein | HC |
chr5:11270980-11267763 | 20130731
Length = 278
Score = 97.4 bits (241), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 76/253 (30%), Positives = 110/253 (43%), Gaps = 28/253 (11%)
Query: 1 MSLFVEHLVQXXXXXXXXXWHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI 60
M F H + WH +I Y V+ P F VR W RLK+LEL +
Sbjct: 1 MGSFKGHALPGTLFFVVGVWHIWASIVRY-VRNPKMFRVRAWNPVPGFDGRLKYLELYVV 59
Query: 61 -------LCFSIL-----AIFTQILDFPNFHYAFKLHNFEHATMFIHLALFAGFSLSIEL 108
LC L IF + P+ +++FEH+ M + ++ +L E
Sbjct: 60 AIGAFIDLCVEFLYSTHLKIFVGGVLNPS-----HMNDFEHSGMLLMFFIYGVVALLSEK 114
Query: 109 TDSLDIFSGFVGILASSMFSQELFLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAI 168
T L + G + LA+ FS E L FHST H GLEG+YH ++A A+
Sbjct: 115 TRFLPLQEGVLCFLAAMAFSAEYLLFSFHSTSHKGLEGYYHTLLVFQIGLCILSSIAGAL 174
Query: 169 FPQSFNAALVLSISIIFQGCWFINMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSK 228
P +F A L I++ QG WF F+L+ P +P+GC + K + C S
Sbjct: 175 LPNNFPADLSNGIALALQGIWFYQTAFVLYGP-MLPDGCKIKDNK---------IMCNSV 224
Query: 229 EADFRARGLANLQ 241
+++ R LAN Q
Sbjct: 225 DSEIRGELLANFQ 237
>Medtr8g071140.1 | transmembrane 45B-like protein | LC |
chr8:30184509-30186960 | 20130731
Length = 366
Score = 95.9 bits (237), Expect = 4e-20, Method: Compositional matrix adjust.
Identities = 73/230 (31%), Positives = 102/230 (44%), Gaps = 20/230 (8%)
Query: 20 WHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSI-------LCFSILAIFTQI 72
WH +++ Y P F VR W R+KHLEL I LC +L I TQ+
Sbjct: 117 WHIWSSVVRY-ASSPKTFQVRVWNPVPGFHGRIKHLELYFISIGVFISLCVELL-IGTQL 174
Query: 73 LDFPN-FHYAFKLHNFEHATMFIHLALFAGFSLSIELTDSLDIFSGFVGILASSMFSQEL 131
F A L+NFEH+ + +F +L E T + + G + + S+ FS E
Sbjct: 175 KIFDGGILNATYLNNFEHSGTMLFSFIFGFVTLLSENTRYVTVPEGALCFIFSTAFSTEY 234
Query: 132 FLLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAIFPQSFNAALVLSISIIFQGCWFI 191
L ++HST H+GLEG+YH +A A+ P SF L SI+I QG W
Sbjct: 235 ILFYYHSTTHIGLEGYYHILLGFLVRLCILSTIAGALLPTSFPVDLCSSITITLQGIWLY 294
Query: 192 NMGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSKEADFRARGLANLQ 241
F+L+ P + GC M + C S E++ LAN Q
Sbjct: 295 QTAFVLYGPMLL-AGCTMRD---------NIITCHSNESEIHGELLANFQ 334
>Medtr1g112150.1 | T1.3 protein | LC | chr1:50712643-50713536 |
20130731
Length = 297
Score = 90.9 bits (224), Expect = 1e-18, Method: Compositional matrix adjust.
Identities = 67/232 (28%), Positives = 105/232 (45%), Gaps = 19/232 (8%)
Query: 20 WHTINTIKSYLVKGPANFTVRFWFQFNTPLARLKHLELVSILC--FSILAIFTQILD--F 75
WH N +K + + +N ++ FWF T ++R L + C FS IF +
Sbjct: 20 WHLFNYLKLHFL-CSSNTSLPFWFP--TKISRYLELYFIVASCAIFSAAEIFEASSNGTI 76
Query: 76 PNFHYAFKLHNFEHATMFIHLALFAGFSLSIELTDSL---DIFSGFVGILASSMFSQELF 132
P H +H EH ++ + ++A F++ ++ S +LA+ F QE
Sbjct: 77 PTNH----IHQVEHTSITLSFLIYATFAIILDTKKSTITEKTQYEITLLLAAIAFGQEFL 132
Query: 133 LLHFHSTDHVGLEGHYHWXXXXXXXXXXXXAMAAAIFPQSFNAALVLSISIIFQGCWFIN 192
L H HS H+G+EG YH+ + FP+SF + S+S+IFQG + +
Sbjct: 133 LFHLHSRSHMGIEGQYHYCLQVLIMIGFFTTLMGIGFPKSFLVCFLRSVSMIFQGLFIMF 192
Query: 193 MGFMLWIPACIPEGCVMNTAKPNQSRILGAVACGSKEADFRARGLANLQFSW 244
MG +L P P+GC + + C ++A RA L NLQFSW
Sbjct: 193 MGLVLSRPGYQPKGCFLEYEGDQL-----MIRCNDEKAMHRAISLVNLQFSW 239