Miyakogusa Predicted Gene

Lj3g3v3237870.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v3237870.1 Non Characterized Hit- tr|I1LJW0|I1LJW0_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.44605 PE,90.23,0,TRNA
(5-METHYLAMINOMETHYL-2-THIOURIDYLATE)-METHYLTRANSFERASE,tRNA-specific
2-thiouridylase; tRNA_Me_,CUFF.45491.1
         (267 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr4g065023.1 | tRNA-specific 2-thiouridylase MnmA | HC | chr4...   493   e-140

>Medtr4g065023.1 | tRNA-specific 2-thiouridylase MnmA | HC |
           chr4:24369688-24373706 | 20130731
          Length = 484

 Score =  493 bits (1270), Expect = e-140,   Method: Compositional matrix adjust.
 Identities = 235/267 (88%), Positives = 248/267 (92%)

Query: 1   MGFDYVASGHYAKVIHPLEDQMDELSVLELSQDMVKDQTYFLSHLSQSQLKRLLFPLGCI 60
           MGFDYVASGHYAKVIHP  D+MD  S LELS+D VKDQTYFLSHLSQSQLKRLLFPLG I
Sbjct: 184 MGFDYVASGHYAKVIHPFADEMDGSSALELSEDTVKDQTYFLSHLSQSQLKRLLFPLGSI 243

Query: 61  PKDEVRRLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGIILEAETGDFLGKH 120
           PKDEVRRLA KFDLPNKDRKDSQGICFLGKI+FSEFVARHIGE+EGIILEAETGDFLGKH
Sbjct: 244 PKDEVRRLAAKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDFLGKH 303

Query: 121 RGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRIFRVGSLKWFSGL 180
           RGFWFYTIGQRQGL+L GGPWYVVEKDVKNNVVFVSRNYFSFDK+RR+FRVGS KW SG+
Sbjct: 304 RGFWFYTIGQRQGLKLSGGPWYVVEKDVKNNVVFVSRNYFSFDKKRRVFRVGSFKWLSGM 363

Query: 181 PPSPTSQLKCKVRHGPGFYNCSLQMEVASDNQVDAAVVHISEDDQGLAAGQFAAFYEGRT 240
           PPS TSQL+CKVRHGPGFY+CSLQME+  DN +D+AVV ISEDDQGLAAGQF AFYEGR 
Sbjct: 364 PPSQTSQLRCKVRHGPGFYDCSLQMELREDNSIDSAVVCISEDDQGLAAGQFTAFYEGRI 423

Query: 241 CIGSGVILESWDDQSFPVCAKALEIAR 267
           CIGSGVILES DDQSFPVCAKALEIAR
Sbjct: 424 CIGSGVILESGDDQSFPVCAKALEIAR 450