Miyakogusa Predicted Gene

Lj3g3v2779500.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v2779500.1 Non Characterized Hit- tr|G7L312|G7L312_MEDTR
Putative uncharacterized protein OS=Medicago
truncatul,52.34,1e-17,Translation proteins,Translation
elongation/initiation factor/Ribosomal, beta-barrel; Gar1,H/ACA
rib,CUFF.44617.1
         (704 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr0072s0050.1 | Gar1/Naf1 RNA-binding region protein | HC | s...   251   2e-66
Medtr2g090095.1 | Gar1/Naf1 RNA-binding region protein | LC | ch...   242   1e-63
Medtr2g090095.2 | Gar1/Naf1 RNA-binding region protein | LC | ch...   209   6e-54
Medtr7g116780.1 | Gar1/Naf1 RNA-binding region protein | LC | ch...    93   8e-19

>Medtr0072s0050.1 | Gar1/Naf1 RNA-binding region protein | HC |
           scaffold0072:33818-29673 | 20130731
          Length = 567

 Score =  251 bits (641), Expect = 2e-66,   Method: Compositional matrix adjust.
 Identities = 154/300 (51%), Positives = 193/300 (64%), Gaps = 23/300 (7%)

Query: 175 PVMSKNDDELEVLPWVPPVNVTLGPHHQMLPVGVVMSILGAQVIVEGVEKHDPLNEGSIL 234
           P+ SKN  E++ LP VPPV+V L PHH+MLPVGVVMS LGAQVIVEGVEKH+PLNEGS+L
Sbjct: 175 PIKSKN--EIQNLPPVPPVDVCLAPHHKMLPVGVVMSTLGAQVIVEGVEKHEPLNEGSVL 232

Query: 235 WMTESRRPLGFIEEIFGPVEHPYYIVRYNSENEVPEGIRGG-TLISFVPEFANHVLRDEE 293
           W+TESR+PLG ++EIFGPV++PYY+VRYNSEN+VP GI+GG TL+S VPEFA HVL +++
Sbjct: 233 WLTESRKPLGLVDEIFGPVKNPYYVVRYNSENDVPAGIQGGTTLVSCVPEFAEHVLNNKD 292

Query: 294 LYKKGYXXXXXXXXXX------XXXGKEAEYKRMQTMAKRGIQDQNPGKRKNN------R 341
           LY+KGY                    KEAEY++MQ M KRG+ DQNPGK+KNN      +
Sbjct: 293 LYRKGYDASGAYDEELFDEVEFSDDEKEAEYRKMQKMTKRGVNDQNPGKKKNNKKKFSPK 352

Query: 342 NEVPSRNAFV------PTLPVAPATS-LPDHGHYSSIPGPGGQGHFSASXXXXXXXXXXX 394
             V   NA V      P +P APA +   + G+YS   G  GQG  +             
Sbjct: 353 EHVLPPNAPVASPHVFPNVPNAPAAAPFVNKGNYSPFSG-IGQGGTTTVSPFQPLNAGPN 411

Query: 395 XXLTTSGVGTSGTALPQQFAVPPNGFPTNDVSWYQGNTQNFHQFPAPGIPFQQQLNSVHG 454
               +     +      Q ++PPN FP+N +S+Y  NTQ  HQFP PGIPFQQQ+N  HG
Sbjct: 412 FAANSMWANQTTFPQQPQPSLPPNAFPSNAMSYYPQNTQFSHQFPVPGIPFQQQMNPSHG 471



 Score = 72.8 bits (177), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 54/133 (40%), Positives = 66/133 (49%), Gaps = 19/133 (14%)

Query: 586 NPVHGYPNNTQISHQFPVQGTLFQQQSNPVNGFPPATTFPGAQPNMFA-QPMYAQRPVNQ 644
           N +  YP NTQ SHQFPV G  FQQQ NP +G    T  PG QPN+F+ QPMYA   + Q
Sbjct: 440 NAMSYYPQNTQFSHQFPVPGIPFQQQMNPSHGTHFPTMMPGVQPNIFSQQPMYAPGFLGQ 499

Query: 645 NQITPGLSTP-------------FPQDQSPINMHSSFISGNQHAPHQFNPGPSANYRRKK 691
           NQ+                    FP  +  +    S ISGN   P QF+PG S +  R  
Sbjct: 500 NQMPQQSFQQIQQPPIFQAQQQGFPPTE--LQQRPSPISGN---PSQFHPGSSGSRGRPS 554

Query: 692 YNRPGKRGWRPGK 704
           +     RGWRP +
Sbjct: 555 FRGASGRGWRPSR 567


>Medtr2g090095.1 | Gar1/Naf1 RNA-binding region protein | LC |
           chr2:38174085-38171015 | 20130731
          Length = 631

 Score =  242 bits (617), Expect = 1e-63,   Method: Compositional matrix adjust.
 Identities = 153/332 (46%), Positives = 182/332 (54%), Gaps = 32/332 (9%)

Query: 167 GVLDDAGRPVMSKNDDELEVLPWVPPVNVTLGPHHQMLPVGVVMSILGAQVIVEGVEKHD 226
           G  DD          +ELE L  VPPVNV LGPHH+MLPVGVV SI+G +VIVEGVEKHD
Sbjct: 139 GDNDDDQSQSQESRTNELENLSRVPPVNVNLGPHHKMLPVGVVTSIVGVRVIVEGVEKHD 198

Query: 227 PLNEGSILWMTESRRPLGFIEEIFGPVEHPYYIVRYNSENEVPEGIRGGTLISFVPEFAN 286
           PLNEGSILW+TE + PLG I+E+FG V++PYY+VRYNSE EVPEGIR GTLISFV EF N
Sbjct: 199 PLNEGSILWITERQTPLGLIDEVFGQVKNPYYVVRYNSEKEVPEGIREGTLISFVAEFVN 258

Query: 287 HVLRDEELYKKGYXXX------XXXXXXXXXXGKEAEYKRMQTMAKRGIQDQNPGKR-KN 339
           HVL +++LYKKGY                    KEAEYKR+    KRG  +Q+  +R +N
Sbjct: 259 HVLNNKDLYKKGYDASGQYDEEVSDEEEFSDDEKEAEYKRLVKQNKRGRNNQSTDRRMEN 318

Query: 340 NRNEVPSRNAFVPTLPVAPATSLPDHGHYSSIPGPGGQGHFSASXXXXXXXXXXXXXLTT 399
           NR +VP ++  + T+ +A A S   HGH   I    GQG   A+                
Sbjct: 319 NRKQVPLKDGSITTMSIAHAGSSVAHGHCPPIQAT-GQGFIGAAHGHSSPLPCTRQSFFG 377

Query: 400 SGVGT----SGTALPQQFA--VPPNG--FPTNDVSWYQGNTQNFHQFPAPGIPFQQQLNS 451
           S             P  F   V PNG   P    + Y  NTQ   Q P  GIP QQQL  
Sbjct: 378 STNVNPPFPHANGGPNVFTTGVWPNGGTLPPQQSAMYPENTQISQQLPMQGIPLQQQL-- 435

Query: 452 VHGYPNNTQISHQVPVPGIPFQQQLNPVHGYP 483
                         P+ GIPFQQQ NP   +P
Sbjct: 436 --------------PMQGIPFQQQFNPSQRFP 453


>Medtr2g090095.2 | Gar1/Naf1 RNA-binding region protein | LC |
           chr2:38173234-38170993 | 20130731
          Length = 457

 Score =  209 bits (533), Expect = 6e-54,   Method: Compositional matrix adjust.
 Identities = 135/296 (45%), Positives = 162/296 (54%), Gaps = 32/296 (10%)

Query: 203 MLPVGVVMSILGAQVIVEGVEKHDPLNEGSILWMTESRRPLGFIEEIFGPVEHPYYIVRY 262
           MLPVGVV SI+G +VIVEGVEKHDPLNEGSILW+TE + PLG I+E+FG V++PYY+VRY
Sbjct: 1   MLPVGVVTSIVGVRVIVEGVEKHDPLNEGSILWITERQTPLGLIDEVFGQVKNPYYVVRY 60

Query: 263 NSENEVPEGIRGGTLISFVPEFANHVLRDEELYKKGYXXX------XXXXXXXXXXGKEA 316
           NSE EVPEGIR GTLISFV EF NHVL +++LYKKGY                    KEA
Sbjct: 61  NSEKEVPEGIREGTLISFVAEFVNHVLNNKDLYKKGYDASGQYDEEVSDEEEFSDDEKEA 120

Query: 317 EYKRMQTMAKRGIQDQNPGKR-KNNRNEVPSRNAFVPTLPVAPATSLPDHGHYSSIPGPG 375
           EYKR+    KRG  +Q+  +R +NNR +VP ++  + T+ +A A S   HGH   I    
Sbjct: 121 EYKRLVKQNKRGRNNQSTDRRMENNRKQVPLKDGSITTMSIAHAGSSVAHGHCPPIQA-T 179

Query: 376 GQGHFSASXXXXXXXXXXXXXLTTSGVGT----SGTALPQQF--AVPPNG--FPTNDVSW 427
           GQG   A+                S             P  F   V PNG   P    + 
Sbjct: 180 GQGFIGAAHGHSSPLPCTRQSFFGSTNVNPPFPHANGGPNVFTTGVWPNGGTLPPQQSAM 239

Query: 428 YQGNTQNFHQFPAPGIPFQQQLNSVHGYPNNTQISHQVPVPGIPFQQQLNPVHGYP 483
           Y  NTQ   Q P  GIP QQQL                P+ GIPFQQQ NP   +P
Sbjct: 240 YPENTQISQQLPMQGIPLQQQL----------------PMQGIPFQQQFNPSQRFP 279


>Medtr7g116780.1 | Gar1/Naf1 RNA-binding region protein | LC |
           chr7:48245064-48244526 | 20130731
          Length = 130

 Score = 93.2 bits (230), Expect = 8e-19,   Method: Composition-based stats.
 Identities = 54/132 (40%), Positives = 77/132 (58%), Gaps = 10/132 (7%)

Query: 227 PLNEGSILWMTESRRPLGFIEEIFGPVEHPYYIVRYNSENEVPEGIRGGTLISFVPEFAN 286
           PL EGSILW+TE +  LG I+EIFG V++PYY VRYNSE    +GIR GTLISFV EF N
Sbjct: 5   PLIEGSILWITERQTSLGLIDEIFGQVKNPYYAVRYNSE----KGIREGTLISFVAEFVN 60

Query: 287 HVLRDEELYKKGYXXXXXXXXXXXXXGKEAEYKRMQTMAKRGIQDQNPGKR-KNNRNEVP 345
           +V+    + K+                K    +  + + K  +  + P +R +NN  ++P
Sbjct: 61  YVMHPVSMMKR-----CPMKQNFWMMRKRLSTRDCKNIIKEVVTIKTPIERMENNIKQIP 115

Query: 346 SRNAFVPTLPVA 357
            ++  +PT+PVA
Sbjct: 116 LKDGSIPTMPVA 127