Miyakogusa Predicted Gene

Lj3g3v2769380.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v2769380.1 Non Characterized Hit- tr|I1M3W9|I1M3W9_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.18832 PE,92.03,0,no
description,Zinc finger, RING/FYVE/PHD-type; coiled-coil,NULL;
VACUOLAR MEMBRANE PROTEIN PEP3,NUL,CUFF.44621.1
         (990 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g063380.1 | vacuolar sorting-associated-like protein | HC ...  1900   0.0  
Medtr5g026090.1 | vacuolar sorting-associated-like protein | LC ...  1383   0.0  
Medtr8g471030.1 | vacuolar sorting-associated-like protein | HC ...   262   1e-69

>Medtr8g063380.1 | vacuolar sorting-associated-like protein | HC |
           chr8:26642088-26651844 | 20130731
          Length = 999

 Score = 1900 bits (4922), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 916/1006 (91%), Positives = 945/1006 (93%), Gaps = 24/1006 (2%)

Query: 1   MDQGRQVFTVDLLERYAAKGRGVITCMTAGNDVIVIGTSRGWVIRHDFGVGDSHEFDLSA 60
           MD GRQVFTVDLLERYAAKG GVITCM AGNDVIVIGTSRGWVIRHDFG GDSHEFDLSA
Sbjct: 1   MDHGRQVFTVDLLERYAAKGHGVITCMAAGNDVIVIGTSRGWVIRHDFGAGDSHEFDLSA 60

Query: 61  GRPGDQSIHRVFVDPGGSHCIATVVGPGGAETFYTHAKWTKPRVLSKLKGLVVNAVAWNR 120
           GRPGDQSIHRVFVDPGG HCIATVVGPGGAETFYTHAKWTKPRVLSKLKGLVVN VAWNR
Sbjct: 61  GRPGDQSIHRVFVDPGGCHCIATVVGPGGAETFYTHAKWTKPRVLSKLKGLVVNVVAWNR 120

Query: 121 QQITEVSTKEVILGTENGQLHELAVDEKDKKEKYIKFLFELAELPEAIMGLQMETASIIN 180
           QQITEVSTKEVILGT+NGQLHELAVDEKDKKEKYIKFL+EL ELPEA+MGLQMETAS+IN
Sbjct: 121 QQITEVSTKEVILGTDNGQLHELAVDEKDKKEKYIKFLYELTELPEALMGLQMETASVIN 180

Query: 181 GTRYYIMAVTPTRLYSFTGFGSLETVFSSYLDRTVHFMELPGDIPNSELHFYIKQRRAVH 240
            TRYY+MAVTPTRLYSFTGFGSLETVF+ YLDR VHFMELPGDIPNSELHFYIKQRRAVH
Sbjct: 181 ETRYYVMAVTPTRLYSFTGFGSLETVFAGYLDRAVHFMELPGDIPNSELHFYIKQRRAVH 240

Query: 241 FAWLSGAGIYHGGLNFGGQNSSASGNENFIENKALLDYSKLSEGTEAVKPSSMALSEFHF 300
           FAWLSGAGIYHGGLNFGGQ+S   GN NFIENKALL+YSKLSEG EAVKPSS+ALSEFHF
Sbjct: 241 FAWLSGAGIYHGGLNFGGQHS---GNGNFIENKALLNYSKLSEGVEAVKPSSIALSEFHF 297

Query: 301 XXXXGNKVKVVNRISENIIEELQFDQTSDSASKGIIGLCSDATAGLFYAYDQNSIFQVSI 360
                NKVKVVNRISENIIEELQFDQTSDSA+KGIIGLCSDATAGLFYAYDQNSIFQVSI
Sbjct: 298 LLLLENKVKVVNRISENIIEELQFDQTSDSAAKGIIGLCSDATAGLFYAYDQNSIFQVSI 357

Query: 361 NDEGRDMWKVYLNMKEYAAALANCRDPFQRDQVYLVQAEAAFSSKDYFRAASFYAKINYI 420
           NDEGRDMWKVYL+M EYAAALANCRDPFQRDQVYLVQAEAAFSSKDYFRA+SFYAKINYI
Sbjct: 358 NDEGRDMWKVYLDMNEYAAALANCRDPFQRDQVYLVQAEAAFSSKDYFRASSFYAKINYI 417

Query: 421 LSFEEVTLKFISAGEQDALRTFLLRKLDNLEKGDKCQITMISTWTTELYLDKVNRLLLED 480
           LSFEEVTLKFISAGEQDALRTFLLRKLDNLEK DKCQITMISTW TELYLDK+NRLLLED
Sbjct: 418 LSFEEVTLKFISAGEQDALRTFLLRKLDNLEKDDKCQITMISTWATELYLDKINRLLLED 477

Query: 481 DSALENSNSEYQSIIQEFRAFLSDSKDVLDEATTMKLLESYGRVEELVYFASLKGQFEIV 540
           DS+LENS+SEYQSII+EFRAFLSDSKDVLDEATTMKLLESYGRVEE VYFASLKGQ+EIV
Sbjct: 478 DSSLENSDSEYQSIIKEFRAFLSDSKDVLDEATTMKLLESYGRVEETVYFASLKGQYEIV 537

Query: 541 VHHY----------------IQQGEAKRALEVLQKPSVPVDLQYKFAPDLITLDAYETVE 584
           VHHY                IQQGEAKRALEVLQKPSV VDLQYKFAPDLI LDAYETVE
Sbjct: 538 VHHYIQAVSLLLFFLFCSFLIQQGEAKRALEVLQKPSVSVDLQYKFAPDLIALDAYETVE 597

Query: 585 SWMTTKNLNPRKLIPAMMRYSSEPHAKNETHEVIKYLEYCVHRLHNEDPGVHNLLLSLYA 644
           SWMTTKNLNPRKLIPAMMRYSSEPHAKNETHEVIKYLE+CVH+LHNEDPGVHNLLLSLYA
Sbjct: 598 SWMTTKNLNPRKLIPAMMRYSSEPHAKNETHEVIKYLEFCVHKLHNEDPGVHNLLLSLYA 657

Query: 645 KQEDDSSLLRFLQCKFGKGPDNGPEFFYDPKYALRLLLKEKRMRACVHIYSMMSMHEEAV 704
           KQEDDSSLLRFL+CKFGKGP+NGPEFFYDPKYALRL LKEKRMRACVHIYSMMSMHEEAV
Sbjct: 658 KQEDDSSLLRFLECKFGKGPENGPEFFYDPKYALRLCLKEKRMRACVHIYSMMSMHEEAV 717

Query: 705 ALALQVDPELAMAEADKVEDDEDLRKKLWLMIAKHVVEQEKGTKRENIRKAIAFLKETDG 764
           ALALQVDPELAMAEADKVEDD DLRKKLWLMIAKHVVEQEKGTKRENIR AIAFLKETDG
Sbjct: 718 ALALQVDPELAMAEADKVEDD-DLRKKLWLMIAKHVVEQEKGTKRENIRMAIAFLKETDG 776

Query: 765 LLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLKEEMNDATHGADNIRNDISALA 824
           LLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLKEEMND THGADNIRNDISALA
Sbjct: 777 LLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLKEEMNDTTHGADNIRNDISALA 836

Query: 825 QRCAVIDRDEECGVCRRKILTAGREFGIGRGYTSVGQMASFYVFPCGHSFHAQCLIAHVT 884
           QRC VIDRDEECGVCRRKIL AGREFGIGRGYTSVGQMA FYVFPCGH+FHAQCLIAHVT
Sbjct: 837 QRCTVIDRDEECGVCRRKILNAGREFGIGRGYTSVGQMAPFYVFPCGHAFHAQCLIAHVT 896

Query: 885 RCTVEAHAEYILDLQKQLTLIGSEARRESNGTLSSEESIPSTITVEKLRSQLDDAIASEC 944
           RCTVE HAEYILDLQKQLTLI SE RRESNG L+SE SIPST +V+KLRSQLDDAIASEC
Sbjct: 897 RCTVETHAEYILDLQKQLTLISSETRRESNGNLASEGSIPSTTSVDKLRSQLDDAIASEC 956

Query: 945 PFCGDLMIREISLSFILPEEEQHVLSWEIKPEIKPNVGSQRSITLS 990
           PFCGDLMIREISL FILP+EEQHVLSW    ++KPNVGSQR+I LS
Sbjct: 957 PFCGDLMIREISLPFILPDEEQHVLSW----DLKPNVGSQRNIPLS 998


>Medtr5g026090.1 | vacuolar sorting-associated-like protein | LC |
            chr5:10719369-10706969 | 20130731
          Length = 1706

 Score = 1383 bits (3580), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 683/787 (86%), Positives = 705/787 (89%), Gaps = 48/787 (6%)

Query: 220  LPGDIPNSELHFYIKQRRAVHFAWLSGAGIYHGGLNFGGQNSSASGNENFIENKALLDYS 279
            L GD    ELHFYIKQRRAVHFAWLSGAGIYHGG                    ALL YS
Sbjct: 951  LSGD----ELHFYIKQRRAVHFAWLSGAGIYHGG--------------------ALLKYS 986

Query: 280  KLSEGTEAVKPSSMALSEFHFXXXXGNKVKVVNRISENIIEELQFDQTSDSASKGIIGLC 339
            KLSEG EAVKPSS+ALSEFHF     NKVKVVNRISENIIEELQFD+TSDSA+KGIIGLC
Sbjct: 987  KLSEGVEAVKPSSIALSEFHFLLLLENKVKVVNRISENIIEELQFDKTSDSAAKGIIGLC 1046

Query: 340  SDATAGLFYAYDQNSIFQVSINDEGRDMWKVYLNMKEYAAALANCRDPFQRDQVYLVQAE 399
            SDATAGLFYAYDQNSIFQVSINDEGRDMWKVYL+M EYAAALANCRDPFQRDQVYLVQ  
Sbjct: 1047 SDATAGLFYAYDQNSIFQVSINDEGRDMWKVYLDMNEYAAALANCRDPFQRDQVYLVQLR 1106

Query: 400  AAFSSKDYFRAASFYAKINYILSFEEVTLKFISAGEQDALRTFLLRKLDNLEKGDKCQIT 459
               SSKDYFRA+SFYAKINYILSFEEVTLKFISAGEQDALRTFLLRKLDNLEK DKCQIT
Sbjct: 1107 ---SSKDYFRASSFYAKINYILSFEEVTLKFISAGEQDALRTFLLRKLDNLEKDDKCQIT 1163

Query: 460  MISTWTTELYLDKVNRLLLEDDSALENSNSEYQSIIQEFRAFLSDSKDVLDEATTMKLLE 519
            MISTW TELYLDK+NRLLLEDDS+LENS+SEYQSII+EFRAFLSDSKDVLDEATTMKLLE
Sbjct: 1164 MISTWATELYLDKINRLLLEDDSSLENSDSEYQSIIKEFRAFLSDSKDVLDEATTMKLLE 1223

Query: 520  SYGRVEELVYFASLKGQFEIVVHHYIQ----------------QGEAKRALEVLQKPSVP 563
            SYGRVEE VYFASLKGQ+EIVVHHYIQ                QGEAKRALEVLQKPSV 
Sbjct: 1224 SYGRVEETVYFASLKGQYEIVVHHYIQAVSLLLFFLFCSFLIQQGEAKRALEVLQKPSVS 1283

Query: 564  VDLQYKFAPDLITLDAYETVESWMTTKNLNPRKLIPAMMRYSSEPHAKNETHEVIKYLEY 623
            VDLQYKFAPDLI LDAYETVESWMTTKNLNPRKLIPAMMRYSSEPHAKNETHEVIKYLE+
Sbjct: 1284 VDLQYKFAPDLIALDAYETVESWMTTKNLNPRKLIPAMMRYSSEPHAKNETHEVIKYLEF 1343

Query: 624  CVHRLHNEDPGVHNLLLSLYAKQEDDSSLLRFLQCKFGKGPDNGPEFFYDPKYALRLLLK 683
            CVH+LHNEDPGVHNLLLSLYAKQEDDSSLLRFL+CKFGKGP+NGPEFFYDPKYALRL LK
Sbjct: 1344 CVHKLHNEDPGVHNLLLSLYAKQEDDSSLLRFLECKFGKGPENGPEFFYDPKYALRLCLK 1403

Query: 684  EKRMRACVHIYSMMSMHEEAVALALQVDPELAMAEADKVEDDEDLRKKLWLMIAKHVVEQ 743
            EKRMRACVHIYSMMSMHEEAVALALQVDPELAMAEADKVED EDLRKKLWLMIAKHVVEQ
Sbjct: 1404 EKRMRACVHIYSMMSMHEEAVALALQVDPELAMAEADKVED-EDLRKKLWLMIAKHVVEQ 1462

Query: 744  EKGTKRENIRKAIAFLKETDGLLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLK 803
            EKGTKRENIR AIAFLKETDG+LKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLK
Sbjct: 1463 EKGTKRENIRMAIAFLKETDGMLKIEDILPFFPDFALIDDFKEAICSSLEDYNKQIEQLK 1522

Query: 804  EEMNDATHGADNIRNDISALAQRCAVIDRDEECGVCRRKILTAGREFGIGRGYTSVGQMA 863
            EEMND THGADNIRNDI ALAQRC VIDRDEECGVCRRKIL AGREFGIG GYTSVGQMA
Sbjct: 1523 EEMNDTTHGADNIRNDIRALAQRCTVIDRDEECGVCRRKILNAGREFGIGCGYTSVGQMA 1582

Query: 864  SFYVFPCGHSFHAQCLIAHVTRCTVEAHAEYILDLQKQLTLIGSEARRESNGTLSSEESI 923
             FYVFPCGH+FH QCLIAHVTRCTVE HAEYILDLQKQLTLI SE  RESNG L+SE SI
Sbjct: 1583 PFYVFPCGHAFHTQCLIAHVTRCTVETHAEYILDLQKQLTLISSETSRESNGNLASEGSI 1642

Query: 924  PSTITVEKLRSQLDDAIASECPFCGDLMIREISLSFILPEEEQHVLSWEIKPEIKPNVGS 983
            PST +V+KLRSQLDDAIASECPFCGDLMIREISL FILPEEEQHVLSW    ++KPNVGS
Sbjct: 1643 PSTTSVDKLRSQLDDAIASECPFCGDLMIREISLPFILPEEEQHVLSW----DLKPNVGS 1698

Query: 984  QRSITLS 990
            QR+I LS
Sbjct: 1699 QRNIPLS 1705


>Medtr8g471030.1 | vacuolar sorting-associated-like protein | HC |
           chr8:26638566-26639740 | 20130731
          Length = 127

 Score =  262 bits (670), Expect = 1e-69,   Method: Composition-based stats.
 Identities = 119/127 (93%), Positives = 120/127 (94%)

Query: 1   MDQGRQVFTVDLLERYAAKGRGVITCMTAGNDVIVIGTSRGWVIRHDFGVGDSHEFDLSA 60
           MD GRQVFTVDLLERYAAKG GVITCM AGNDVIVIGTSRGWVIRHDFG GDSHEFDLSA
Sbjct: 1   MDHGRQVFTVDLLERYAAKGHGVITCMAAGNDVIVIGTSRGWVIRHDFGAGDSHEFDLSA 60

Query: 61  GRPGDQSIHRVFVDPGGSHCIATVVGPGGAETFYTHAKWTKPRVLSKLKGLVVNAVAWNR 120
           GRPGDQSIHRVFVDPGG HCIATVVGPGGAETFYT+AKWTKPRVLSKLKGLVVN VAWNR
Sbjct: 61  GRPGDQSIHRVFVDPGGCHCIATVVGPGGAETFYTYAKWTKPRVLSKLKGLVVNVVAWNR 120

Query: 121 QQITEVS 127
           QQITE S
Sbjct: 121 QQITEGS 127