Miyakogusa Predicted Gene

Lj3g3v2656760.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v2656760.1 Non Characterized Hit- tr|I1K622|I1K622_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.55586
PE,90.14,0,coiled-coil,NULL; AA_TRNA_LIGASE_II,Aminoacyl-tRNA
synthetase, class II; WHEP_TRS_2,WHEP-TRS; asnS: ,CUFF.44329.1
         (364 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g102050.1 | cytoplasmic asparagine-tRNA ligase | HC | chr8...   681   0.0  
Medtr8g102050.2 | cytoplasmic asparagine-tRNA ligase | HC | chr8...   681   0.0  
Medtr7g101050.1 | cytoplasmic asparagine-tRNA ligase | HC | chr7...   647   0.0  
Medtr4g100920.1 | asparagine-tRNA ligase | HC | chr4:41665503-41...   381   e-106
Medtr8g011530.1 | asparagine-tRNA ligase | HC | chr8:3134854-313...   350   1e-96
Medtr4g100920.2 | asparagine-tRNA ligase | HC | chr4:41665479-41...   224   1e-58
Medtr1g014140.1 | aspartate-tRNA ligase | HC | chr1:3102208-3107...   108   1e-23
Medtr5g028360.1 | aspartate-tRNA ligase | HC | chr5:11868501-118...   105   6e-23

>Medtr8g102050.1 | cytoplasmic asparagine-tRNA ligase | HC |
           chr8:42909869-42915043 | 20130731
          Length = 551

 Score =  681 bits (1757), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 322/364 (88%), Positives = 349/364 (95%)

Query: 1   MFQVTTLLSEAERLEKELIHNPPPTEADVEAARLIVQEKGGIVSQLKSAKARKQEISASV 60
           MFQVTTL SEAERLEKELI NPPPTEADVEAA+L+VQEKG +VSQLKSAKA K+EISA+V
Sbjct: 188 MFQVTTLFSEAERLEKELIQNPPPTEADVEAAKLVVQEKGEVVSQLKSAKADKKEISAAV 247

Query: 61  DDLKKAKESLSKVEERSKLKPGIPQKDGKVDYTRDFFARQAFLTVSGQLQVESYACALSS 120
            +LKKAKE++SK+EERSKL+PGIP+KDGKVDYT+DFFARQAFLTVSGQLQVESYACALS 
Sbjct: 248 AELKKAKENVSKLEERSKLQPGIPRKDGKVDYTKDFFARQAFLTVSGQLQVESYACALSR 307

Query: 121 VYTFGPTFRAENSHTTRHLAEFWMVEPEIAFADLEDDMNCAEAYVKFLCQWLLDSCLEDM 180
           VYTFGPTFRAENSHT+RHLAEFWMVEPEIAFADLEDDM CAEAYVKFLC+WLLD+CLEDM
Sbjct: 308 VYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMKCAEAYVKFLCRWLLDNCLEDM 367

Query: 181 EFMADKFDKSCIDRLKMVASTPFIRISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHE 240
           EFMADKFDK CIDRLKMVASTPFIR++YTEAVE+L E+V+NGKKFE EV+WGIDLASEHE
Sbjct: 368 EFMADKFDKGCIDRLKMVASTPFIRLTYTEAVEILEESVQNGKKFEKEVEWGIDLASEHE 427

Query: 241 RYLTEVKFQKPVIVYNYPKDIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYDV 300
           RYLTEVK+QKPVIVYNYPKDIKAFYMRLNDD +TVAAMDVLVPKVGELIGGSQREERYDV
Sbjct: 428 RYLTEVKYQKPVIVYNYPKDIKAFYMRLNDDLETVAAMDVLVPKVGELIGGSQREERYDV 487

Query: 301 IVKRISEMGLPLEPYEWYLDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFSRYPG 360
           I +R+ EM LP+EPYEWY+DLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPF RYPG
Sbjct: 488 IQQRLEEMDLPVEPYEWYIDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFPRYPG 547

Query: 361 RADL 364
           RADL
Sbjct: 548 RADL 551


>Medtr8g102050.2 | cytoplasmic asparagine-tRNA ligase | HC |
           chr8:42909869-42915043 | 20130731
          Length = 551

 Score =  681 bits (1757), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 322/364 (88%), Positives = 349/364 (95%)

Query: 1   MFQVTTLLSEAERLEKELIHNPPPTEADVEAARLIVQEKGGIVSQLKSAKARKQEISASV 60
           MFQVTTL SEAERLEKELI NPPPTEADVEAA+L+VQEKG +VSQLKSAKA K+EISA+V
Sbjct: 188 MFQVTTLFSEAERLEKELIQNPPPTEADVEAAKLVVQEKGEVVSQLKSAKADKKEISAAV 247

Query: 61  DDLKKAKESLSKVEERSKLKPGIPQKDGKVDYTRDFFARQAFLTVSGQLQVESYACALSS 120
            +LKKAKE++SK+EERSKL+PGIP+KDGKVDYT+DFFARQAFLTVSGQLQVESYACALS 
Sbjct: 248 AELKKAKENVSKLEERSKLQPGIPRKDGKVDYTKDFFARQAFLTVSGQLQVESYACALSR 307

Query: 121 VYTFGPTFRAENSHTTRHLAEFWMVEPEIAFADLEDDMNCAEAYVKFLCQWLLDSCLEDM 180
           VYTFGPTFRAENSHT+RHLAEFWMVEPEIAFADLEDDM CAEAYVKFLC+WLLD+CLEDM
Sbjct: 308 VYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMKCAEAYVKFLCRWLLDNCLEDM 367

Query: 181 EFMADKFDKSCIDRLKMVASTPFIRISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHE 240
           EFMADKFDK CIDRLKMVASTPFIR++YTEAVE+L E+V+NGKKFE EV+WGIDLASEHE
Sbjct: 368 EFMADKFDKGCIDRLKMVASTPFIRLTYTEAVEILEESVQNGKKFEKEVEWGIDLASEHE 427

Query: 241 RYLTEVKFQKPVIVYNYPKDIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYDV 300
           RYLTEVK+QKPVIVYNYPKDIKAFYMRLNDD +TVAAMDVLVPKVGELIGGSQREERYDV
Sbjct: 428 RYLTEVKYQKPVIVYNYPKDIKAFYMRLNDDLETVAAMDVLVPKVGELIGGSQREERYDV 487

Query: 301 IVKRISEMGLPLEPYEWYLDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFSRYPG 360
           I +R+ EM LP+EPYEWY+DLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPF RYPG
Sbjct: 488 IQQRLEEMDLPVEPYEWYIDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFPRYPG 547

Query: 361 RADL 364
           RADL
Sbjct: 548 RADL 551


>Medtr7g101050.1 | cytoplasmic asparagine-tRNA ligase | HC |
           chr7:40762406-40766030 | 20130731
          Length = 576

 Score =  647 bits (1669), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 312/369 (84%), Positives = 343/369 (92%), Gaps = 8/369 (2%)

Query: 1   MFQVTTLLSEAERLEKELIHNPPPTEADVEAARLIVQEKGGIVSQLKSAKARKQEISASV 60
           MFQVTTL SEAERLEKELI NPPPTE+DVEAA+L+VQEKG +VSQLK+A+ +++EI+A+V
Sbjct: 211 MFQVTTLFSEAERLEKELIQNPPPTESDVEAAKLVVQEKGEVVSQLKTAEEKEKEITAAV 270

Query: 61  DDLKKAKESLSKVEERSKLKPGIPQKDGKVDYTRDFFARQAFLTVSGQLQVESYACALSS 120
           D+LKKAK ++SK+EERSKL+P IP+KDGK+DY +DFFARQAFLTVSGQLQVESYACALSS
Sbjct: 271 DELKKAKRNVSKLEERSKLQPSIPRKDGKIDYAKDFFARQAFLTVSGQLQVESYACALSS 330

Query: 121 VYTFGPTFRAENSHTTRHLAEFWMVEPEIAFADLEDDMNCAEAYVKFLCQWLLDSCLEDM 180
           VYTFGPTFRAENSHT+RHLAEFWMVEPEIAFADLEDDM CAEAYVKFLCQWLLD+CLEDM
Sbjct: 331 VYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMKCAEAYVKFLCQWLLDNCLEDM 390

Query: 181 EFMADKFDKSCIDRLKMVASTPFIRISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHE 240
           EFMA   DK CIDRLKMVASTPFIR++YTEAVE+L EAVKNG +FENEVKWGIDLASEHE
Sbjct: 391 EFMA---DKGCIDRLKMVASTPFIRLTYTEAVEILEEAVKNGTEFENEVKWGIDLASEHE 447

Query: 241 RYLTEVKFQ-KPVIVYNYPK--DI--KAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQRE 295
           RYLTEVK+Q KPVIVYNYPK  DI  KAFYMRLNDD KTVAAMDVLVP VGELIGG QRE
Sbjct: 448 RYLTEVKYQKKPVIVYNYPKGEDIKGKAFYMRLNDDLKTVAAMDVLVPNVGELIGGGQRE 507

Query: 296 ERYDVIVKRISEMGLPLEPYEWYLDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPF 355
           ERYDVI +R+ EM LP+EPYEWY+DLRRYGTVKHAGFGLGFERMILFATGL+NIRDVIPF
Sbjct: 508 ERYDVIQQRLKEMDLPVEPYEWYIDLRRYGTVKHAGFGLGFERMILFATGLKNIRDVIPF 567

Query: 356 SRYPGRADL 364
            RYPGRADL
Sbjct: 568 PRYPGRADL 576


>Medtr4g100920.1 | asparagine-tRNA ligase | HC |
           chr4:41665503-41658740 | 20130731
          Length = 546

 Score =  381 bits (979), Expect = e-106,   Method: Compositional matrix adjust.
 Identities = 182/284 (64%), Positives = 221/284 (77%), Gaps = 4/284 (1%)

Query: 82  GIPQKDGKVDYTRDFFARQAFLTVSGQLQVESYACALSSVYTFGPTFRAENSHTTRHLAE 141
            IP+ D  +D+++DFF + AFLTVSGQL  E+YA ALS VYTFGPTFRAENS+T+RHLAE
Sbjct: 266 AIPKNDKLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAE 325

Query: 142 FWMVEPEIAFADLEDDMNCAEAYVKFLCQWLLDSCLEDMEFMADKFDKSCIDRLKMVAST 201
           FWM+EPE+AFADL DDM CA AY++F+ + +LD+C EDMEF     DK  IDRL  VA  
Sbjct: 326 FWMIEPELAFADLNDDMACATAYLQFVIRHILDNCKEDMEFFNTWIDKGIIDRLSDVAEK 385

Query: 202 PFIRISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHERYLTEVKFQK-PVIVYNYPKD 260
             ++I+YTEAV++L  A    KKFE  VKWG DL SEHERY+TE  F   PVI+ +YPKD
Sbjct: 386 DVLQITYTEAVDLLSRA---NKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPKD 442

Query: 261 IKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYDVIVKRISEMGLPLEPYEWYLD 320
           IKAFYMR NDD KTVAAMD+LVPK+GELIGGSQREER + +  R+ ++ L  + Y WYLD
Sbjct: 443 IKAFYMRQNDDGKTVAAMDMLVPKIGELIGGSQREERLEHLEARLDDLKLNKDAYWWYLD 502

Query: 321 LRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFSRYPGRADL 364
           LRRYG+V HAGFGLGFER++ FATG++NIRDVIPF R PG A+ 
Sbjct: 503 LRRYGSVPHAGFGLGFERLVQFATGMDNIRDVIPFPRTPGSAEF 546


>Medtr8g011530.1 | asparagine-tRNA ligase | HC |
           chr8:3134854-3130862 | 20130731
          Length = 633

 Score =  350 bits (898), Expect = 1e-96,   Method: Compositional matrix adjust.
 Identities = 178/366 (48%), Positives = 253/366 (69%), Gaps = 10/366 (2%)

Query: 1   MFQVTTLLSEAERLEKELIHNPPPTEA-DVEAARLIVQEKGGIVSQLKSAKARKQEISAS 59
           MFQVTT +++  +  KE +     TE   +E  +   +EK  +V  LK +++ ++ ++A+
Sbjct: 274 MFQVTTTVNQ--KAVKEKLSTIYETEGVSLEIVKEAAKEKSKLVETLKRSESNREALAAA 331

Query: 60  VDDLKKAKESLSKVEERSKLKPGIPQKDGKVDYTRDFFARQAFLTVSGQLQVESYACALS 119
           + DLKK  E  S++E R K K G   KD K+D ++DFF+ Q +LTVSG+L +ESYACAL 
Sbjct: 332 IQDLKKTNELASQLEVREKKKLGTSLKDEKLDTSKDFFSSQTYLTVSGRLHLESYACALG 391

Query: 120 SVYTFGPTFRAENSHTTRHLAEFWMVEPEIAFADLEDDMNCAEAYVKFLCQWLLDSCLED 179
           +VY+FGP F A+ + + +H AE WMVE E+AFA+L+D MNCA    K+LC+ +L++C ED
Sbjct: 392 NVYSFGPRFHADKTDSAKHAAEMWMVEAEMAFAELKDSMNCANDLFKYLCERVLENCSED 451

Query: 180 MEFMADKFDKSCIDRLKMVASTPFIRISYTEAVEVLVEA--VKNGKKFENEVKWGIDLAS 237
           M+F+A + D +CIDRL+ + S     ISY EA++V ++A   K+  KF++    G+ L S
Sbjct: 452 MKFVAKRIDHTCIDRLRQIISGSPEMISYDEALDVFIKAEDEKSKTKFDS----GLKLTS 507

Query: 238 EHERYLTEVKFQKPVIVYNYPKDIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREER 297
           +H  YL +  ++KPVI+++YPK+ K FY+R+NDD KTVAA D +VPKVG +I GSQ EER
Sbjct: 508 DHLSYLVDAIYKKPVIIHSYPKEAKPFYVRVNDD-KTVAAFDFVVPKVGTIISGSQNEER 566

Query: 298 YDVIVKRISEMGLPLEPYEWYLDLRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFSR 357
             VI  RI+E+GLP E YEWYLDL R GTVK +GF L F+ M+LF TGL N+RDVIPF R
Sbjct: 567 LTVINSRITELGLPREKYEWYLDLCRNGTVKRSGFTLRFDLMVLFTTGLSNVRDVIPFPR 626

Query: 358 YPGRAD 363
              +A+
Sbjct: 627 SYDKAN 632


>Medtr4g100920.2 | asparagine-tRNA ligase | HC |
           chr4:41665479-41658740 | 20130731
          Length = 463

 Score =  224 bits (570), Expect = 1e-58,   Method: Compositional matrix adjust.
 Identities = 109/179 (60%), Positives = 134/179 (74%), Gaps = 4/179 (2%)

Query: 82  GIPQKDGKVDYTRDFFARQAFLTVSGQLQVESYACALSSVYTFGPTFRAENSHTTRHLAE 141
            IP+ D  +D+++DFF + AFLTVSGQL  E+YA ALS VYTFGPTFRAENS+T+RHLAE
Sbjct: 266 AIPKNDKLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAE 325

Query: 142 FWMVEPEIAFADLEDDMNCAEAYVKFLCQWLLDSCLEDMEFMADKFDKSCIDRLKMVAST 201
           FWM+EPE+AFADL DDM CA AY++F+ + +LD+C EDMEF     DK  IDRL  VA  
Sbjct: 326 FWMIEPELAFADLNDDMACATAYLQFVIRHILDNCKEDMEFFNTWIDKGIIDRLSDVAEK 385

Query: 202 PFIRISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHERYLTEVKFQK-PVIVYNYPK 259
             ++I+YTEAV++L  A    KKFE  VKWG DL SEHERY+TE  F   PVI+ +YPK
Sbjct: 386 DVLQITYTEAVDLLSRA---NKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPK 441


>Medtr1g014140.1 | aspartate-tRNA ligase | HC | chr1:3102208-3107666
           | 20130731
          Length = 541

 Score =  108 bits (269), Expect = 1e-23,   Method: Compositional matrix adjust.
 Identities = 81/281 (28%), Positives = 138/281 (49%), Gaps = 20/281 (7%)

Query: 88  GKVDYTRDFFARQAFLTVSGQLQVESYACA-LSSVYTFGPTFRAENSHTTRHLAEFWMVE 146
           G   +  D+  + A L  S QL  +   C     V+  GP FRAE+S T RHL EF  ++
Sbjct: 271 GAAVFRLDYKGQPACLAQSPQLHKQMSICGDFGRVFEIGPVFRAEDSFTHRHLCEFTGLD 330

Query: 147 PEIAFA-DLEDDMNCAEAYVKFLCQWLLDSCLEDMEFMADKFDKSCIDRLKMVASTPFIR 205
            E+       + M+  +     +   L   C +D+E +A+++     + LK + +T  +R
Sbjct: 331 VEMEIKKHYFEVMDVVDKLFVAMFDTLNTKCKKDLEAVANQYP---FEPLKYLPNT--LR 385

Query: 206 ISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHERYLTEVKFQKP----VIVYNYPKDI 261
           ++Y E +++L       K+   E++   DL +E ER L ++  +K      I++ YP  +
Sbjct: 386 LTYEEGIQML-------KEVGVEIEPFGDLNTEAERKLGQLVLEKYGTEFYILHRYPLAV 438

Query: 262 KAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERYDVIVKRISEMGLPLEPYEWYLD 320
           + FY M   D+     + DV + +  E+I G+QR    + + +R    G+ ++    Y+D
Sbjct: 439 RPFYTMPCYDNPNYSNSFDVFI-RGEEIISGAQRIHVPEFLEERAQACGIEVKTISAYID 497

Query: 321 LRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFSRYPGR 361
             RYG   H GFG+G ER+++   GL NIR    + R P R
Sbjct: 498 SFRYGVPPHGGFGVGLERVVMLFCGLNNIRKTSLYPRDPLR 538


>Medtr5g028360.1 | aspartate-tRNA ligase | HC |
           chr5:11868501-11872196 | 20130731
          Length = 589

 Score =  105 bits (263), Expect = 6e-23,   Method: Compositional matrix adjust.
 Identities = 80/281 (28%), Positives = 140/281 (49%), Gaps = 20/281 (7%)

Query: 88  GKVDYTRDFFARQAFLTVSGQLQVESYACA-LSSVYTFGPTFRAENSHTTRHLAEFWMVE 146
           G   +  ++    A L  S QL  +   C   + V+  G  +RAE+S T RHL EF  ++
Sbjct: 319 GASVFKLNYKGHPACLAQSPQLHKQIAICGDFNRVFEVGAVYRAEDSFTHRHLCEFTGLD 378

Query: 147 PEIAF-ADLEDDMNCAEAYVKFLCQWLLDSCLEDMEFMADKFDKSCIDRLKMVASTPFIR 205
            E+       + M+  +     +   L   C +D+E + +++     + LK + +T  +R
Sbjct: 379 AEMRIDCHYFEIMDIVDRLFVAIFDCLNQKCKKDLEAVRNQYP---FEPLKYLRNT--LR 433

Query: 206 ISYTEAVEVLVEAVKNGKKFENEVKWGIDLASEHERYLTEV---KFQKP-VIVYNYPKDI 261
           ++Y E +E+L       K+   E++   DL +E ER L ++   K++K   I++ YP  +
Sbjct: 434 LTYEEGIEML-------KEVGVEIEPFGDLNTEAERKLGQLVLEKYEKEFCILHRYPLAV 486

Query: 262 KAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERYDVIVKRISEMGLPLEPYEWYLD 320
           + FY M  +DD     + DV + +  E+I G+QR     ++ +R    G+ ++   +Y++
Sbjct: 487 RPFYSMPCSDDQNYSNSFDVFI-RGEEIISGAQRIHEPKLLEERTQACGINVDAISYYIE 545

Query: 321 LRRYGTVKHAGFGLGFERMILFATGLENIRDVIPFSRYPGR 361
             RYG   H GFG G ER+++   GL+NIR    F R P R
Sbjct: 546 AFRYGAPLHGGFGAGLERVVMLFCGLDNIRMTSLFPRDPKR 586