Miyakogusa Predicted Gene

Lj3g3v2531230.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v2531230.1 Non Characterized Hit- tr|J9JB62|J9JB62_9SPIT
Membrane protein, putative OS=Oxytricha trifallax
PE=4,32.47,2e-18,DUF1295,Protein of unknown function DUF1295;
seg,NULL; S5A_REDUCTASE,3-oxo-5-alpha-steroid 4-dehydro,CUFF.44144.1
         (335 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g023760.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...   537   e-153
Medtr7g117475.1 | phospholipid methyltransferase | HC | chr7:486...   429   e-120
Medtr8g107030.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    61   2e-09
Medtr8g107030.2 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    61   2e-09
Medtr8g107030.3 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    56   6e-08

>Medtr8g023760.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:8635853-8638859 | 20130731
          Length = 329

 Score =  537 bits (1384), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 262/335 (78%), Positives = 288/335 (85%), Gaps = 6/335 (1%)

Query: 1   MSHDRNVKNAVIALLVPLPSILFYLSFLNHYDSSLATESDPSFQSSWSTLWAWCYHHPXX 60
           M  ++N+KNA+IA+LVPLPSI+FYL+FLN+Y SS++  ++PSF   +STLW WCYHHP  
Sbjct: 1   MVCNQNLKNAIIAILVPLPSIIFYLTFLNNYHSSISIPNNPSF---FSTLWTWCYHHPLL 57

Query: 61  XXXXXXXXXXXXXXWVIGQIQSSHWMIDPYWTVIPVMLAHYYATHPLAHYDWWRSRIVIL 120
                         W+IGQIQSSHW+IDPYWTVIPVML HYYATHPLA YD WRS+IVIL
Sbjct: 58  FANALFFFNVNIIFWLIGQIQSSHWLIDPYWTVIPVMLVHYYATHPLAQYDLWRSKIVIL 117

Query: 121 LTWVWSIRLTHNYFRREKWQWGAREDWRFTEMSQQYGKQWWWVSFFAVYVSQQMFLIGLS 180
           LTWVWSIRLTHNYFRREKWQWGAREDWRFT+MSQQYGK WWWVSFFAVYVSQQ+FLIGLS
Sbjct: 118 LTWVWSIRLTHNYFRREKWQWGAREDWRFTQMSQQYGKLWWWVSFFAVYVSQQIFLIGLS 177

Query: 181 LPFYVIHSVNHPLSIWDLVAIVVCLCGIVMAYFADTQLHDFMSRNNKLKGLGKPLVPVLD 240
           LP YV+H VN PLSI DLVAIVVCL GIV+AYFADTQLHDFMSRNN+LKGLGKP++PVLD
Sbjct: 178 LPLYVVHFVNKPLSILDLVAIVVCLSGIVIAYFADTQLHDFMSRNNQLKGLGKPVIPVLD 237

Query: 241 NGLWYYCRHPNYVGEQLWWWGLVVFAWSLGYGWTFIGALVNTLCLAYVTRLVEDRMLKQE 300
            GLWYY R PNY GE LWWWGLVVFAW+LG+GWTFIGALVNT CLAYV RLVEDRMLKQE
Sbjct: 238 TGLWYYSRRPNYFGETLWWWGLVVFAWNLGHGWTFIGALVNTFCLAYVARLVEDRMLKQE 297

Query: 301 SRAEAFRLYQKTTSVWIPWFKSSPSGLSSVKNKNA 335
           SRAEAF+LYQKTTS WIPWFKS P   S VKNKNA
Sbjct: 298 SRAEAFKLYQKTTSAWIPWFKSFP---SDVKNKNA 329


>Medtr7g117475.1 | phospholipid methyltransferase | HC |
           chr7:48618001-48616890 | 20130731
          Length = 314

 Score =  429 bits (1102), Expect = e-120,   Method: Compositional matrix adjust.
 Identities = 219/340 (64%), Positives = 249/340 (73%), Gaps = 31/340 (9%)

Query: 1   MSHDRNVKNAVIALLVPLPSILFYLSFLNHYDSSLATESDPSFQSSWSTLWAWCYHHPXX 60
           M    N+KNAVIA L PLPSI+FYLSF+N+        +D  F          CY HP  
Sbjct: 1   MGCTSNLKNAVIAFLAPLPSIIFYLSFINN-------ATDHPF----------CYRHPLL 43

Query: 61  XXXXXXXXXXXXXXWVIGQIQSSHWMIDPYWTVIPVMLAHYYATHPLAHYDWWRSRIVIL 120
                         W+I  IQSSHWMIDPYWTVIPVML HYYA HPLAHYD+WRSRIVIL
Sbjct: 44  LVNLLFFFNVNILFWLIALIQSSHWMIDPYWTVIPVMLVHYYAAHPLAHYDFWRSRIVIL 103

Query: 121 LTWVWSIRLTHNYFRREKWQWGAREDWRFTEMSQQYGKQWWWVSFFAVYVSQQMFLIGLS 180
           LTWVWSIRL HNYFRREKWQWGAREDWRFT+M QQYGK WWW SFF++Y+  Q+ LIGLS
Sbjct: 104 LTWVWSIRLIHNYFRREKWQWGAREDWRFTDMIQQYGKHWWWASFFSIYLPHQLLLIGLS 163

Query: 181 LPFYVIHSVNHPLSIWDLVAIVVCLCGIVMAYFADTQLHDF--MSRNNKLKGLGKPLVPV 238
           LPFYVIHSVN PL+IWDLVA+VVC+ GI++AYFAD QL++F  M  +N +         +
Sbjct: 164 LPFYVIHSVNQPLNIWDLVAVVVCVSGILIAYFADNQLYNFVMMMMSNDM---------I 214

Query: 239 LDNGLWYYCRHPNYVGEQLWWWGLVVFAWSLGYGWTFIGALVNTLCLAYVTRLVEDRMLK 298
           L++GLWYY RHPNY GEQLWWWGLVVF W+LGYGWTFIGAL+NT+CL YVT+LVE RMLK
Sbjct: 215 LESGLWYYTRHPNYFGEQLWWWGLVVFGWNLGYGWTFIGALLNTMCLGYVTKLVEQRMLK 274

Query: 299 QESRAEAFRLYQKTTSVWIPWFK---SSPSGLSSVKNKNA 335
           Q+ R EA+R YQ TTSVWIP FK   SS   L   K KNA
Sbjct: 275 QDERVEAYRKYQNTTSVWIPCFKFNSSSYPNLPLNKTKNA 314


>Medtr8g107030.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:45212143-45214354 | 20130731
          Length = 307

 Score = 60.8 bits (146), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 57/187 (30%), Positives = 93/187 (49%), Gaps = 24/187 (12%)

Query: 140 QWGAREDWRFTEMSQQYGKQWWWVSFFAVY-VSQQMFLIGLSLPFYVIHSV--NHPLSIW 196
           QWG  ED RF +M    GK        A++ + Q +++  +SLP  V+++   N  L   
Sbjct: 87  QWG--EDRRFDQMRNNLGK-------LAIFWIFQAVWVWAVSLPVTVVNATDRNPFLQSV 137

Query: 197 DLVAIVVCLCGIVMAYFADTQLHDFMSRNNKLKGLGKPLVPVLDNGLWYYCRHPNYVGEQ 256
           DL+  ++   G ++   AD Q  +F  R+ + +G         + GLW Y RHPNY GE 
Sbjct: 138 DLIGWLMWAVGFMIEGTADQQKLNF-KRSPENRG------KWCNAGLWKYTRHPNYFGEI 190

Query: 257 LWWWGLVVFAWSL---GYGWTFIGALVNTLCLAYVTR--LVEDRMLKQESRAEAFRLYQK 311
           L WWG+ V +  +         IG +  TL L +++   L+E+   K+    + +R+Y++
Sbjct: 191 LLWWGIFVASTPILDRAEWLVIIGPIFLTLLLLFISGIPLLEESADKKFGNVDGYRVYKQ 250

Query: 312 TTSVWIP 318
            TS  IP
Sbjct: 251 RTSPLIP 257


>Medtr8g107030.2 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:45212143-45214354 | 20130731
          Length = 338

 Score = 60.8 bits (146), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 57/187 (30%), Positives = 93/187 (49%), Gaps = 24/187 (12%)

Query: 140 QWGAREDWRFTEMSQQYGKQWWWVSFFAVY-VSQQMFLIGLSLPFYVIHSV--NHPLSIW 196
           QWG  ED RF +M    GK        A++ + Q +++  +SLP  V+++   N  L   
Sbjct: 118 QWG--EDRRFDQMRNNLGK-------LAIFWIFQAVWVWAVSLPVTVVNATDRNPFLQSV 168

Query: 197 DLVAIVVCLCGIVMAYFADTQLHDFMSRNNKLKGLGKPLVPVLDNGLWYYCRHPNYVGEQ 256
           DL+  ++   G ++   AD Q  +F  R+ + +G         + GLW Y RHPNY GE 
Sbjct: 169 DLIGWLMWAVGFMIEGTADQQKLNF-KRSPENRG------KWCNAGLWKYTRHPNYFGEI 221

Query: 257 LWWWGLVVFAWSL---GYGWTFIGALVNTLCLAYVTR--LVEDRMLKQESRAEAFRLYQK 311
           L WWG+ V +  +         IG +  TL L +++   L+E+   K+    + +R+Y++
Sbjct: 222 LLWWGIFVASTPILDRAEWLVIIGPIFLTLLLLFISGIPLLEESADKKFGNVDGYRVYKQ 281

Query: 312 TTSVWIP 318
            TS  IP
Sbjct: 282 RTSPLIP 288


>Medtr8g107030.3 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:45212143-45214658 | 20130731
          Length = 253

 Score = 55.8 bits (133), Expect = 6e-08,   Method: Compositional matrix adjust.
 Identities = 53/182 (29%), Positives = 90/182 (49%), Gaps = 24/182 (13%)

Query: 140 QWGAREDWRFTEMSQQYGKQWWWVSFFAVY-VSQQMFLIGLSLPFYVIHSVNHP--LSIW 196
           QWG  ED RF +M    GK        A++ + Q +++  +SLP  V+++ +    L   
Sbjct: 87  QWG--EDRRFDQMRNNLGK-------LAIFWIFQAVWVWAVSLPVTVVNATDRNPFLQSV 137

Query: 197 DLVAIVVCLCGIVMAYFADTQLHDFMSRNNKLKGLGKPLVPVLDNGLWYYCRHPNYVGEQ 256
           DL+  ++   G ++   AD Q  +F  R+ + +G         + GLW Y RHPNY GE 
Sbjct: 138 DLIGWLMWAVGFMIEGTADQQKLNF-KRSPENRG------KWCNAGLWKYTRHPNYFGEI 190

Query: 257 LWWWGLVVFAWSL---GYGWTFIGALVNTLCLAYVTR--LVEDRMLKQESRAEAFRLYQK 311
           L WWG+ V +  +         IG +  TL L +++   L+E+   K+    + +R+Y++
Sbjct: 191 LLWWGIFVASTPILDRAEWLVIIGPIFLTLLLLFISGIPLLEESADKKFGNVDGYRVYKQ 250

Query: 312 TT 313
            T
Sbjct: 251 RT 252