Miyakogusa Predicted Gene

Lj3g3v2517640.3
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v2517640.3 Non Characterized Hit- tr|I3SFM8|I3SFM8_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,99.13,0,CDF:
cation diffusion facilitator family transport,Cation efflux protein;
Cation_efflux,Cation efflu,CUFF.44120.3
         (347 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g024240.2 | heavy metal transporter MTP1 | HC | chr8:88889...   305   4e-83
Medtr8g024240.1 | heavy metal transporter MTP1 | HC | chr8:88881...   305   4e-83
Medtr2g036390.3 | cation efflux protein/zinc transporter | HC | ...   297   1e-80
Medtr2g036390.1 | cation efflux protein/zinc transporter | HC | ...   297   1e-80
Medtr2g036390.2 | cation efflux protein/zinc transporter | HC | ...   297   1e-80
Medtr4g064893.1 | cation diffusion facilitator family transporte...   150   1e-36
Medtr6g463330.1 | cation diffusion facilitator family transporte...    65   1e-10
Medtr7g093290.1 | metal tolerance-like protein | HC | chr7:37063...    49   9e-06

>Medtr8g024240.2 | heavy metal transporter MTP1 | HC |
           chr8:8888988-8885947 | 20130731
          Length = 385

 Score =  305 bits (781), Expect = 4e-83,   Method: Compositional matrix adjust.
 Identities = 149/175 (85%), Positives = 165/175 (94%), Gaps = 1/175 (0%)

Query: 1   MEAQSSQHGQVIEIGGDLCEE-RKICGEAPCGFADAGAISKDSEERSTAMRKLLMAVILC 59
           M+A SSQHGQ+IEI  DL +  RKIC E+PCGF+DAG+ISKDS+ERST+MRKL+MAVILC
Sbjct: 1   MDAHSSQHGQIIEINADLPDAGRKICCESPCGFSDAGSISKDSKERSTSMRKLMMAVILC 60

Query: 60  VIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEANPRQSYGFFRIEI 119
           VIFMTVE+VGG+KANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEA PRQSYGFFRIEI
Sbjct: 61  VIFMTVELVGGLKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQSYGFFRIEI 120

Query: 120 LGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFLMFMVAAFGLVVNILMAFLL 174
           LGALVSIQLIWLLAGI+VYEAIDR+IAGP++VDGFLMF VAAFGLVVNI+MA LL
Sbjct: 121 LGALVSIQLIWLLAGIIVYEAIDRLIAGPQNVDGFLMFSVAAFGLVVNIIMAVLL 175



 Score =  187 bits (476), Expect = 9e-48,   Method: Compositional matrix adjust.
 Identities = 91/100 (91%), Positives = 96/100 (96%), Gaps = 1/100 (1%)

Query: 248 VTEPLLGESKDRPKKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTL 307
           VTEPLLGESK++  KKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEW+IVDLICTL
Sbjct: 227 VTEPLLGESKEK-SKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWKIVDLICTL 285

Query: 308 IFSVIVLGTTINMLRNILEVLMESTPREIDATELERGVLE 347
            FS IVLGTTINMLRNILEVLMESTPREIDAT+LERG+LE
Sbjct: 286 FFSAIVLGTTINMLRNILEVLMESTPREIDATKLERGILE 325


>Medtr8g024240.1 | heavy metal transporter MTP1 | HC |
           chr8:8888188-8885983 | 20130731
          Length = 385

 Score =  305 bits (781), Expect = 4e-83,   Method: Compositional matrix adjust.
 Identities = 149/175 (85%), Positives = 165/175 (94%), Gaps = 1/175 (0%)

Query: 1   MEAQSSQHGQVIEIGGDLCEE-RKICGEAPCGFADAGAISKDSEERSTAMRKLLMAVILC 59
           M+A SSQHGQ+IEI  DL +  RKIC E+PCGF+DAG+ISKDS+ERST+MRKL+MAVILC
Sbjct: 1   MDAHSSQHGQIIEINADLPDAGRKICCESPCGFSDAGSISKDSKERSTSMRKLMMAVILC 60

Query: 60  VIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEANPRQSYGFFRIEI 119
           VIFMTVE+VGG+KANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEA PRQSYGFFRIEI
Sbjct: 61  VIFMTVELVGGLKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQSYGFFRIEI 120

Query: 120 LGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFLMFMVAAFGLVVNILMAFLL 174
           LGALVSIQLIWLLAGI+VYEAIDR+IAGP++VDGFLMF VAAFGLVVNI+MA LL
Sbjct: 121 LGALVSIQLIWLLAGIIVYEAIDRLIAGPQNVDGFLMFSVAAFGLVVNIIMAVLL 175



 Score =  187 bits (476), Expect = 9e-48,   Method: Compositional matrix adjust.
 Identities = 91/100 (91%), Positives = 96/100 (96%), Gaps = 1/100 (1%)

Query: 248 VTEPLLGESKDRPKKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTL 307
           VTEPLLGESK++  KKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEW+IVDLICTL
Sbjct: 227 VTEPLLGESKEK-SKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWKIVDLICTL 285

Query: 308 IFSVIVLGTTINMLRNILEVLMESTPREIDATELERGVLE 347
            FS IVLGTTINMLRNILEVLMESTPREIDAT+LERG+LE
Sbjct: 286 FFSAIVLGTTINMLRNILEVLMESTPREIDATKLERGILE 325


>Medtr2g036390.3 | cation efflux protein/zinc transporter | HC |
           chr2:15756787-15761166 | 20130731
          Length = 407

 Score =  297 bits (760), Expect = 1e-80,   Method: Compositional matrix adjust.
 Identities = 145/175 (82%), Positives = 162/175 (92%), Gaps = 1/175 (0%)

Query: 1   MEAQSSQHGQVIEIGGDLCE-ERKICGEAPCGFADAGAISKDSEERSTAMRKLLMAVILC 59
           MEAQS+ HGQ+IEI G+L +  RKICGEAPC F+DAG+ISKDSEERST+MRKL +AV LC
Sbjct: 1   MEAQSAHHGQIIEISGELPDVGRKICGEAPCEFSDAGSISKDSEERSTSMRKLFIAVTLC 60

Query: 60  VIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEANPRQSYGFFRIEI 119
           VIFM VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFS+WA GWE NPRQS+GFFRIEI
Sbjct: 61  VIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSIWAGGWEPNPRQSFGFFRIEI 120

Query: 120 LGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFLMFMVAAFGLVVNILMAFLL 174
           LGALVS+QLIWLLAGILVYEAI R+IAGP++VDGFLMF+VAAFGLVVNI+MA +L
Sbjct: 121 LGALVSMQLIWLLAGILVYEAIARLIAGPQEVDGFLMFVVAAFGLVVNIIMALVL 175



 Score =  188 bits (478), Expect = 5e-48,   Method: Compositional matrix adjust.
 Identities = 89/100 (89%), Positives = 96/100 (96%)

Query: 248 VTEPLLGESKDRPKKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTL 307
           VTEPLLGESK R +KKRNINV GAYLHVLGDSIQSIGVMIGGA+IWYKPEW+IVDLICTL
Sbjct: 248 VTEPLLGESKGRSEKKRNINVHGAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIVDLICTL 307

Query: 308 IFSVIVLGTTINMLRNILEVLMESTPREIDATELERGVLE 347
           IFSVIVL TTINMLRNILEVLMESTPREIDAT+L++G+LE
Sbjct: 308 IFSVIVLATTINMLRNILEVLMESTPREIDATQLQKGLLE 347


>Medtr2g036390.1 | cation efflux protein/zinc transporter | HC |
           chr2:15756787-15761166 | 20130731
          Length = 407

 Score =  297 bits (760), Expect = 1e-80,   Method: Compositional matrix adjust.
 Identities = 145/175 (82%), Positives = 162/175 (92%), Gaps = 1/175 (0%)

Query: 1   MEAQSSQHGQVIEIGGDLCE-ERKICGEAPCGFADAGAISKDSEERSTAMRKLLMAVILC 59
           MEAQS+ HGQ+IEI G+L +  RKICGEAPC F+DAG+ISKDSEERST+MRKL +AV LC
Sbjct: 1   MEAQSAHHGQIIEISGELPDVGRKICGEAPCEFSDAGSISKDSEERSTSMRKLFIAVTLC 60

Query: 60  VIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEANPRQSYGFFRIEI 119
           VIFM VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFS+WA GWE NPRQS+GFFRIEI
Sbjct: 61  VIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSIWAGGWEPNPRQSFGFFRIEI 120

Query: 120 LGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFLMFMVAAFGLVVNILMAFLL 174
           LGALVS+QLIWLLAGILVYEAI R+IAGP++VDGFLMF+VAAFGLVVNI+MA +L
Sbjct: 121 LGALVSMQLIWLLAGILVYEAIARLIAGPQEVDGFLMFVVAAFGLVVNIIMALVL 175



 Score =  188 bits (478), Expect = 5e-48,   Method: Compositional matrix adjust.
 Identities = 89/100 (89%), Positives = 96/100 (96%)

Query: 248 VTEPLLGESKDRPKKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTL 307
           VTEPLLGESK R +KKRNINV GAYLHVLGDSIQSIGVMIGGA+IWYKPEW+IVDLICTL
Sbjct: 248 VTEPLLGESKGRSEKKRNINVHGAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIVDLICTL 307

Query: 308 IFSVIVLGTTINMLRNILEVLMESTPREIDATELERGVLE 347
           IFSVIVL TTINMLRNILEVLMESTPREIDAT+L++G+LE
Sbjct: 308 IFSVIVLATTINMLRNILEVLMESTPREIDATQLQKGLLE 347


>Medtr2g036390.2 | cation efflux protein/zinc transporter | HC |
           chr2:15756791-15761153 | 20130731
          Length = 407

 Score =  297 bits (760), Expect = 1e-80,   Method: Compositional matrix adjust.
 Identities = 145/175 (82%), Positives = 162/175 (92%), Gaps = 1/175 (0%)

Query: 1   MEAQSSQHGQVIEIGGDLCE-ERKICGEAPCGFADAGAISKDSEERSTAMRKLLMAVILC 59
           MEAQS+ HGQ+IEI G+L +  RKICGEAPC F+DAG+ISKDSEERST+MRKL +AV LC
Sbjct: 1   MEAQSAHHGQIIEISGELPDVGRKICGEAPCEFSDAGSISKDSEERSTSMRKLFIAVTLC 60

Query: 60  VIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEANPRQSYGFFRIEI 119
           VIFM VEVVGGIKANSLAILTDAAHLLSDVAAFAISLFS+WA GWE NPRQS+GFFRIEI
Sbjct: 61  VIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSIWAGGWEPNPRQSFGFFRIEI 120

Query: 120 LGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFLMFMVAAFGLVVNILMAFLL 174
           LGALVS+QLIWLLAGILVYEAI R+IAGP++VDGFLMF+VAAFGLVVNI+MA +L
Sbjct: 121 LGALVSMQLIWLLAGILVYEAIARLIAGPQEVDGFLMFVVAAFGLVVNIIMALVL 175



 Score =  188 bits (478), Expect = 5e-48,   Method: Compositional matrix adjust.
 Identities = 89/100 (89%), Positives = 96/100 (96%)

Query: 248 VTEPLLGESKDRPKKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTL 307
           VTEPLLGESK R +KKRNINV GAYLHVLGDSIQSIGVMIGGA+IWYKPEW+IVDLICTL
Sbjct: 248 VTEPLLGESKGRSEKKRNINVHGAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIVDLICTL 307

Query: 308 IFSVIVLGTTINMLRNILEVLMESTPREIDATELERGVLE 347
           IFSVIVL TTINMLRNILEVLMESTPREIDAT+L++G+LE
Sbjct: 308 IFSVIVLATTINMLRNILEVLMESTPREIDATQLQKGLLE 347


>Medtr4g064893.1 | cation diffusion facilitator family transporter |
           HC | chr4:24244048-24246190 | 20130731
          Length = 394

 Score =  150 bits (380), Expect = 1e-36,   Method: Compositional matrix adjust.
 Identities = 69/139 (49%), Positives = 101/139 (72%), Gaps = 5/139 (3%)

Query: 41  DSEERST-----AMRKLLMAVILCVIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAIS 95
            S+E+S      + +KL + ++   I M VE VGG+KA+SL++++DAAHLLSD+  FAIS
Sbjct: 40  SSKEKSVGASNESAKKLSLLIVFYAIVMIVEFVGGLKAHSLSVISDAAHLLSDIGGFAIS 99

Query: 96  LFSLWAAGWEANPRQSYGFFRIEILGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFL 155
           LF++WA+GWEA P QS+G+ R+E+LGAL+S+QLIW ++G L+YEA  R++     V+G L
Sbjct: 100 LFAVWASGWEATPHQSFGYNRLEVLGALISVQLIWFISGFLIYEAFGRLVYKNNRVNGKL 159

Query: 156 MFMVAAFGLVVNILMAFLL 174
           MF +A FG V+N +M   L
Sbjct: 160 MFAIATFGFVLNCIMVLWL 178



 Score =  117 bits (294), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 55/81 (67%), Positives = 64/81 (79%)

Query: 265 NINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTLIFSVIVLGTTINMLRNI 324
           NIN+QGAYLHV+ D IQS GVMI G VIW KPEW IVDL+CTLIFSV+ L TT+ MLRNI
Sbjct: 253 NINIQGAYLHVMTDMIQSFGVMIAGGVIWAKPEWFIVDLLCTLIFSVLSLSTTLPMLRNI 312

Query: 325 LEVLMESTPREIDATELERGV 345
             +LME TP EI+  +LE G+
Sbjct: 313 FGILMERTPSEINVIQLENGL 333


>Medtr6g463330.1 | cation diffusion facilitator family transporter |
           HC | chr6:21913825-21916862 | 20130731
          Length = 905

 Score = 65.1 bits (157), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 36/110 (32%), Positives = 64/110 (58%), Gaps = 4/110 (3%)

Query: 42  SEERSTAMRKLLMAVILCVIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWA 101
           SE +S   RK+ + +++   +M VE V G  +NSL +++DA H+L D AA AI L++ + 
Sbjct: 420 SERKS---RKIALFLLINAGYMVVEFVAGFMSNSLGLISDACHMLFDCAALAIGLYASYI 476

Query: 102 AGWEANPRQSYGFFRIEILGALVSIQLIWLLAGILVYEAIDRMIAGPKDV 151
           +   AN   +YG  R E+L    +   + L+  ++V E+ +R I  P+++
Sbjct: 477 SRLPANNHYNYGRGRFEVLSGYTNAVFLVLVGALIVVESFER-ILDPQEI 525



 Score = 62.0 bits (149), Expect = 9e-10,   Method: Compositional matrix adjust.
 Identities = 29/79 (36%), Positives = 50/79 (63%), Gaps = 1/79 (1%)

Query: 256 SKDRPKKKRNINVQGAYLHVLGDSIQSIGVMIGGAVIWYKPEWQIVDLICTLIFSVIVLG 315
           +K    +  + N++G +LHVL D++ S+GV+I   +I YK  W + D  C++  SV+++ 
Sbjct: 752 NKKHSHRHIDHNMEGIFLHVLADTLGSVGVVISTLLIKYK-GWLVADPACSIFISVLIVS 810

Query: 316 TTINMLRNILEVLMESTPR 334
           + I +LRN  EVL++  PR
Sbjct: 811 SVIPLLRNSAEVLLQRVPR 829


>Medtr7g093290.1 | metal tolerance-like protein | HC |
           chr7:37063412-37072046 | 20130731
          Length = 390

 Score = 48.5 bits (114), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 31/121 (25%), Positives = 60/121 (49%), Gaps = 1/121 (0%)

Query: 49  MRKLLMAVILCVIFMTVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEANP 108
           M++L   + L V + TVE++ G+    + +++DA HL         SLF + A+  + + 
Sbjct: 108 MKRLFFMISLNVAYSTVELLIGLFTGRVGLVSDAVHLTFGCGLLTFSLFVMGASRKKPDG 167

Query: 109 RQSYGFFRIEILGALVSIQLIWLLAGILVYEAIDRMIAGPKDVDGFLMFMVAAFGLVVNI 168
             +YG+ R+E+L A  +   +  ++  L  EA+   I    +   +L  + A   L VN+
Sbjct: 168 DYTYGYKRLEVLSAFTNALFLLFMSFSLAVEALHAFIQDESEHKHYLN-VSAVTNLFVNL 226

Query: 169 L 169
           +
Sbjct: 227 I 227