Miyakogusa Predicted Gene

Lj3g3v1182320.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v1182320.1 tr|G7ITU0|G7ITU0_MEDTR Calpain-B OS=Medicago
truncatula GN=MTR_2g086560 PE=4 SV=1,72.06,0,seg,NULL; EF-hand,
calcium binding motif,Calcium-binding EF-hand; no
description,EF-hand-like domain,CUFF.42332.1
         (253 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr2g086560.1 | calcium-binding EF-hand protein | HC | chr2:36...   315   3e-86
Medtr2g086560.2 | calcium-binding EF-hand protein | HC | chr2:36...   251   4e-67
Medtr2g086560.3 | calcium-binding EF-hand protein | HC | chr2:36...   238   3e-63
Medtr1g051895.1 | calcium-binding EF-hand protein | HC | chr1:20...   197   7e-51
Medtr1g051895.2 | calcium-binding EF-hand protein | HC | chr1:20...   137   1e-32
Medtr2g105130.1 | calcium-binding EF-hand protein | HC | chr2:45...   132   4e-31
Medtr1g051895.3 | calcium-binding EF-hand protein | HC | chr1:20...    71   8e-13

>Medtr2g086560.1 | calcium-binding EF-hand protein | HC |
           chr2:36396297-36391847 | 20130731
          Length = 265

 Score =  315 bits (807), Expect = 3e-86,   Method: Compositional matrix adjust.
 Identities = 147/174 (84%), Positives = 160/174 (91%)

Query: 80  THPEVIRAFQMVDRDQSGFIDEKELQQVLTSGFQKFNIGTIRLLMFLFKHPNEPLRVGPK 139
           T+ +VIR+FQMVDRD+SGFID++ELQQ L+S F  FN+ TIRLLMFLFKHP+E LR+GPK
Sbjct: 92  TNQDVIRSFQMVDRDRSGFIDDRELQQALSSSFHSFNLRTIRLLMFLFKHPHESLRIGPK 151

Query: 140 EFAALWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYSDGTG 199
           EF  LWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVP SVLQLLLSKYSDG  
Sbjct: 152 EFTELWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPASVLQLLLSKYSDGNN 211

Query: 200 RRVELGFDSFVECGMIIKGLTDKFKEKDTRYTGSATLSYDAFMSMVIPFLVSYD 253
           RRVELGFDSFVECGMIIKGLTDKFK+KD RY+GSATL+YD FMSMVIPFLVSYD
Sbjct: 212 RRVELGFDSFVECGMIIKGLTDKFKDKDKRYSGSATLAYDDFMSMVIPFLVSYD 265


>Medtr2g086560.2 | calcium-binding EF-hand protein | HC |
           chr2:36396247-36391884 | 20130731
          Length = 252

 Score =  251 bits (641), Expect = 4e-67,   Method: Compositional matrix adjust.
 Identities = 116/138 (84%), Positives = 126/138 (91%)

Query: 80  THPEVIRAFQMVDRDQSGFIDEKELQQVLTSGFQKFNIGTIRLLMFLFKHPNEPLRVGPK 139
           T+ +VIR+FQMVDRD+SGFID++ELQQ L+S F  FN+ TIRLLMFLFKHP+E LR+GPK
Sbjct: 92  TNQDVIRSFQMVDRDRSGFIDDRELQQALSSSFHSFNLRTIRLLMFLFKHPHESLRIGPK 151

Query: 140 EFAALWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYSDGTG 199
           EF  LWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVP SVLQLLLSKYSDG  
Sbjct: 152 EFTELWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPASVLQLLLSKYSDGNN 211

Query: 200 RRVELGFDSFVECGMIIK 217
           RRVELGFDSFVECGMIIK
Sbjct: 212 RRVELGFDSFVECGMIIK 229


>Medtr2g086560.3 | calcium-binding EF-hand protein | HC |
           chr2:36396297-36392048 | 20130731
          Length = 223

 Score =  238 bits (608), Expect = 3e-63,   Method: Compositional matrix adjust.
 Identities = 110/132 (83%), Positives = 120/132 (90%)

Query: 80  THPEVIRAFQMVDRDQSGFIDEKELQQVLTSGFQKFNIGTIRLLMFLFKHPNEPLRVGPK 139
           T+ +VIR+FQMVDRD+SGFID++ELQQ L+S F  FN+ TIRLLMFLFKHP+E LR+GPK
Sbjct: 92  TNQDVIRSFQMVDRDRSGFIDDRELQQALSSSFHSFNLRTIRLLMFLFKHPHESLRIGPK 151

Query: 140 EFAALWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYSDGTG 199
           EF  LWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVP SVLQLLLSKYSDG  
Sbjct: 152 EFTELWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPASVLQLLLSKYSDGNN 211

Query: 200 RRVELGFDSFVE 211
           RRVELGFDSFVE
Sbjct: 212 RRVELGFDSFVE 223


>Medtr1g051895.1 | calcium-binding EF-hand protein | HC |
           chr1:20793375-20787827 | 20130731
          Length = 305

 Score =  197 bits (502), Expect = 7e-51,   Method: Compositional matrix adjust.
 Identities = 95/172 (55%), Positives = 126/172 (73%), Gaps = 1/172 (0%)

Query: 80  THPEVIRAFQMVDRDQSGFIDEKELQQVLTSGFQKFNIGTIRLLMFLFKHPNEPLRVGPK 139
           T P ++  FQ+ D+D SG ID+KELQ+ L+S  Q F++ T+ LLM+ F + N   ++GPK
Sbjct: 135 TDPSIVACFQVADQDGSGLIDDKELQRALSSYNQSFSLRTVHLLMYHFTNTNV-KKIGPK 193

Query: 140 EFAALWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYSDGTG 199
           EF +L+  L  WRGIFER+DKDRSGKID  ELRDAL  +GYAV   VL LL+SK+    G
Sbjct: 194 EFTSLFYSLQSWRGIFERFDKDRSGKIDSNELRDALLSLGYAVSPVVLDLLVSKFDKTGG 253

Query: 200 RRVELGFDSFVECGMIIKGLTDKFKEKDTRYTGSATLSYDAFMSMVIPFLVS 251
           +   + +D+F+EC + +KGLTDKFKEKDT YTGSAT SY+AFM  V+PFL++
Sbjct: 254 KNKAVEYDNFIECCLTVKGLTDKFKEKDTGYTGSATFSYEAFMLTVLPFLIA 305


>Medtr1g051895.2 | calcium-binding EF-hand protein | HC |
           chr1:20793275-20788287 | 20130731
          Length = 282

 Score =  137 bits (344), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 67/132 (50%), Positives = 92/132 (69%), Gaps = 1/132 (0%)

Query: 80  THPEVIRAFQMVDRDQSGFIDEKELQQVLTSGFQKFNIGTIRLLMFLFKHPNEPLRVGPK 139
           T P ++  FQ+ D+D SG ID+KELQ+ L+S  Q F++ T+ LLM+ F + N   ++GPK
Sbjct: 135 TDPSIVACFQVADQDGSGLIDDKELQRALSSYNQSFSLRTVHLLMYHFTNTNV-KKIGPK 193

Query: 140 EFAALWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYSDGTG 199
           EF +L+  L  WRGIFER+DKDRSGKID  ELRDAL  +GYAV   VL LL+SK+    G
Sbjct: 194 EFTSLFYSLQSWRGIFERFDKDRSGKIDSNELRDALLSLGYAVSPVVLDLLVSKFDKTGG 253

Query: 200 RRVELGFDSFVE 211
           +   + +D+F+E
Sbjct: 254 KNKAVEYDNFIE 265


>Medtr2g105130.1 | calcium-binding EF-hand protein | HC |
           chr2:45335444-45333503 | 20130731
          Length = 127

 Score =  132 bits (331), Expect = 4e-31,   Method: Compositional matrix adjust.
 Identities = 63/112 (56%), Positives = 83/112 (74%)

Query: 138 PKEFAALWNCLGHWRGIFERYDKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYSDG 197
           PKEF +L+  L  WRGIFE +DKDRSG+I+ +ELRDAL  +GYAV   VL LL+SK+   
Sbjct: 11  PKEFTSLFYSLQSWRGIFETFDKDRSGRINSIELRDALLSLGYAVSPMVLDLLVSKFDKT 70

Query: 198 TGRRVELGFDSFVECGMIIKGLTDKFKEKDTRYTGSATLSYDAFMSMVIPFL 249
            G+   + +D+F+EC + +KGLTDKFKEKDT YTG AT SY+  +SM+I +L
Sbjct: 71  GGKSKAIEYDNFIECCLTVKGLTDKFKEKDTGYTGFATFSYEYQLSMLIAWL 122


>Medtr1g051895.3 | calcium-binding EF-hand protein | HC |
           chr1:20793275-20789582 | 20130731
          Length = 259

 Score = 71.2 bits (173), Expect = 8e-13,   Method: Compositional matrix adjust.
 Identities = 33/73 (45%), Positives = 49/73 (67%), Gaps = 1/73 (1%)

Query: 80  THPEVIRAFQMVDRDQSGFIDEKELQQVLTSGFQKFNIGTIRLLMFLFKHPNEPLRVGPK 139
           T P ++  FQ+ D+D SG ID+KELQ+ L+S  Q F++ T+ LLM+ F + N   ++GPK
Sbjct: 135 TDPSIVACFQVADQDGSGLIDDKELQRALSSYNQSFSLRTVHLLMYHFTNTNV-KKIGPK 193

Query: 140 EFAALWNCLGHWR 152
           EF +L+  L  WR
Sbjct: 194 EFTSLFYSLQSWR 206