Miyakogusa Predicted Gene

Lj3g3v1048900.2
Show Alignment:  
BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v1048900.2 Non Characterized Hit- tr|I3SHL4|I3SHL4_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,98.65,0,FMN_HYDROXY_ACID_DH_1,FMN-dependent alpha-hydroxy acid
dehydrogenase, active site; FMN-linked oxidor,CUFF.42212.2
         (371 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr2g070420.1 | peroxisomal (S)-2-hydroxy-acid oxidase GLO1 | ...   697   0.0  
Medtr2g070420.2 | peroxisomal (S)-2-hydroxy-acid oxidase GLO1 | ...   697   0.0  
Medtr1g072440.1 | peroxisomal (S)-2-hydroxy-acid oxidase GLO5 | ...   104   2e-22
Medtr3g076640.1 | FMN-dependent dehydrogenase | LC | chr3:344474...    67   2e-11

>Medtr2g070420.1 | peroxisomal (S)-2-hydroxy-acid oxidase GLO1 | HC
           | chr2:29642100-29646961 | 20130731
          Length = 372

 Score =  697 bits (1799), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 336/370 (90%), Positives = 351/370 (94%)

Query: 2   EVTNVTEYEAIAKQKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDMA 61
           E+TN++EYE IA+QKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKID++
Sbjct: 3   EITNISEYEEIARQKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDLS 62

Query: 62  TTVLGFKISMPIMIAPTAFQKMAHPEGEYXXXXXXXXXGTIMTLSSWATSSVEEVASTGP 121
           TTVLGFKISMPIMIAPTAFQKMAHPEGEY         GTIMTLSSWATSSVEEVASTGP
Sbjct: 63  TTVLGFKISMPIMIAPTAFQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 122

Query: 122 GIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFNLPPYLTLK 181
           GIRFFQLYVYKDRNVVAQLVRRAE+AGFKAIALTVDTPRLGRREADIKNRF LPP+LTLK
Sbjct: 123 GIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 182

Query: 182 NFEGLNLGSMDKADDSGLASYVAGQIDRTLSWQDVKWLQTITKLPILVKGVLTAEDTRIA 241
           NFEGLNLG MD+A+DSGLASYVAGQIDRTLSW+DVKWLQTIT LPILVKGVLTAED R+A
Sbjct: 183 NFEGLNLGKMDEANDSGLASYVAGQIDRTLSWKDVKWLQTITSLPILVKGVLTAEDARLA 242

Query: 242 VQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALG 301
           VQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGR+PVFLDGGVRRGTDVFKALALG
Sbjct: 243 VQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRVPVFLDGGVRRGTDVFKALALG 302

Query: 302 ASGIFIGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAP 361
           ASGIFIGRPVVY+LAAEGE GVRKVLQMLRDEFELTMALSGCRSLKEITRDHI  DWD P
Sbjct: 303 ASGIFIGRPVVYSLAAEGEVGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIVADWDTP 362

Query: 362 RVQPRALPRL 371
           RV PRA+PRL
Sbjct: 363 RVNPRAIPRL 372


>Medtr2g070420.2 | peroxisomal (S)-2-hydroxy-acid oxidase GLO1 | HC
           | chr2:29641871-29646961 | 20130731
          Length = 372

 Score =  697 bits (1799), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 336/370 (90%), Positives = 351/370 (94%)

Query: 2   EVTNVTEYEAIAKQKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDMA 61
           E+TN++EYE IA+QKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKID++
Sbjct: 3   EITNISEYEEIARQKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDLS 62

Query: 62  TTVLGFKISMPIMIAPTAFQKMAHPEGEYXXXXXXXXXGTIMTLSSWATSSVEEVASTGP 121
           TTVLGFKISMPIMIAPTAFQKMAHPEGEY         GTIMTLSSWATSSVEEVASTGP
Sbjct: 63  TTVLGFKISMPIMIAPTAFQKMAHPEGEYATARAASAAGTIMTLSSWATSSVEEVASTGP 122

Query: 122 GIRFFQLYVYKDRNVVAQLVRRAERAGFKAIALTVDTPRLGRREADIKNRFNLPPYLTLK 181
           GIRFFQLYVYKDRNVVAQLVRRAE+AGFKAIALTVDTPRLGRREADIKNRF LPP+LTLK
Sbjct: 123 GIRFFQLYVYKDRNVVAQLVRRAEKAGFKAIALTVDTPRLGRREADIKNRFVLPPFLTLK 182

Query: 182 NFEGLNLGSMDKADDSGLASYVAGQIDRTLSWQDVKWLQTITKLPILVKGVLTAEDTRIA 241
           NFEGLNLG MD+A+DSGLASYVAGQIDRTLSW+DVKWLQTIT LPILVKGVLTAED R+A
Sbjct: 183 NFEGLNLGKMDEANDSGLASYVAGQIDRTLSWKDVKWLQTITSLPILVKGVLTAEDARLA 242

Query: 242 VQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALG 301
           VQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGR+PVFLDGGVRRGTDVFKALALG
Sbjct: 243 VQSGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRVPVFLDGGVRRGTDVFKALALG 302

Query: 302 ASGIFIGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAP 361
           ASGIFIGRPVVY+LAAEGE GVRKVLQMLRDEFELTMALSGCRSLKEITRDHI  DWD P
Sbjct: 303 ASGIFIGRPVVYSLAAEGEVGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIVADWDTP 362

Query: 362 RVQPRALPRL 371
           RV PRA+PRL
Sbjct: 363 RVNPRAIPRL 372


>Medtr1g072440.1 | peroxisomal (S)-2-hydroxy-acid oxidase GLO5 |
          LC | chr1:32146603-32146984 | 20130731
          Length = 88

 Score =  104 bits (259), Expect = 2e-22,   Method: Composition-based stats.
 Identities = 51/82 (62%), Positives = 61/82 (74%), Gaps = 2/82 (2%)

Query: 9  YEAIAKQKLPKMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDMATTVLGFK 68
          YEAIAK+KL K+ +DYYASG ED        N  +R  FRP+IL+DVSKID+ TT+LG  
Sbjct: 4  YEAIAKEKLSKVIYDYYASGVEDYTRFTIYINLRNR--FRPQILMDVSKIDLTTTILGLN 61

Query: 69 ISMPIMIAPTAFQKMAHPEGEY 90
          ISMPI+IAPT  QKMAHPEG +
Sbjct: 62 ISMPIIIAPTILQKMAHPEGTH 83


>Medtr3g076640.1 | FMN-dependent dehydrogenase | LC |
          chr3:34447456-34448279 | 20130731
          Length = 110

 Score = 67.4 bits (163), Expect = 2e-11,   Method: Composition-based stats.
 Identities = 37/74 (50%), Positives = 48/74 (64%), Gaps = 3/74 (4%)

Query: 3  VTNVTEYEAIAKQKLP-KMAFDYYASGAEDQWTLQENRNAFSRILFRPRILIDVSKIDMA 61
          ++  T + +  + K P  +  DYY SGAE Q TLQ+NRNAF RIL  P IL+DVSKID+ 
Sbjct: 4  ISGKTFWRSCGQLKFPASLTNDYYESGAEVQRTLQDNRNAFLRIL--PPILMDVSKIDLT 61

Query: 62 TTVLGFKISMPIMI 75
          TTVLG  +   + I
Sbjct: 62 TTVLGMSLKSLVYI 75