Miyakogusa Predicted Gene
- Lj3g3v0824710.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj3g3v0824710.1 Non Characterized Hit- tr|Q4QFU7|Q4QFU7_LEIMA
Putative uncharacterized protein OS=Leishmania major
G,30.8,2e-18,Choline_transpo,Choline transporter-like; seg,NULL;
PREDICTED PROTEIN,NULL; CTL TRANSPORTER,Choline ,CUFF.41509.1
(561 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr3g033520.2 | plasma-membrane choline transporter family pro... 822 0.0
Medtr3g033520.1 | plasma-membrane choline transporter family pro... 821 0.0
Medtr7g086710.1 | plasma-membrane choline transporter family pro... 206 5e-53
Medtr5g054780.1 | plasma-membrane choline transporter family pro... 119 5e-27
Medtr1g111330.1 | plasma-membrane choline transporter family pro... 116 5e-26
Medtr6g027560.1 | plasma-membrane choline transporter family pro... 107 2e-23
Medtr1g111330.2 | plasma-membrane choline transporter family pro... 74 3e-13
>Medtr3g033520.2 | plasma-membrane choline transporter family
protein | HC | chr3:10739307-10744080 | 20130731
Length = 579
Score = 822 bits (2122), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 401/504 (79%), Positives = 433/504 (85%), Gaps = 5/504 (0%)
Query: 61 FNLSRFNRLNPTNPLRIVINXXXXXXXXXXXXXXXXXXXHPRSIPIPTXXXXXXXXXXXX 120
FN+SRF+RLNPTNPLRIV+N RSIPIPT
Sbjct: 73 FNVSRFHRLNPTNPLRIVMNSNTRVAKPPPPKKSQRSHTPTRSIPIPTPAPIQTPQPPPP 132
Query: 121 XXX-----VTLNSRKYTNRISLFLFVVHMFIAIALVGFLVFKGVQGLILESESMKRKKKN 175
VTLNSR+YTNRISLF+F +H +AIALV FLVFKG+QGL+ ES S+KRK+K
Sbjct: 133 PPPQQQQPVTLNSRRYTNRISLFIFALHQLLAIALVCFLVFKGIQGLVQESGSVKRKEKR 192
Query: 176 MLKFFLPQVEAASFMSIILAFIWQQAIRKWPTFMVHFILWFTFVMSLAAGILLICFQKPP 235
+L +FLPQVEAA+FMSIILAFIWQ AIRKWPTFMVHFILWFTFVMSLAAGILLICFQKPP
Sbjct: 193 VLMYFLPQVEAATFMSIILAFIWQGAIRKWPTFMVHFILWFTFVMSLAAGILLICFQKPP 252
Query: 236 TDGVGVCFIAFAIGNGLYACWASNRIKFCCKILNLSLQPLSKFPDLNKPTYYMLGAGFLW 295
TDGVGVCFIAFAIGNGLY CW S+RIKFCCK+L+LSLQP+SKF DLN+PTYYML AGFLW
Sbjct: 253 TDGVGVCFIAFAIGNGLYGCWISHRIKFCCKVLSLSLQPMSKFSDLNRPTYYMLAAGFLW 312
Query: 296 ISLWSLAVIGSLNFYFPPLVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSSTQ 355
ISLW+LAV+G+LNFYFPPLVI+AL LSLAWTTEVMRNVVNITVSRVIALYYLRGMQSSTQ
Sbjct: 313 ISLWTLAVVGALNFYFPPLVIIALVLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSSTQ 372
Query: 356 FCFLRALTRNLGSACLGSLFVPAIEXXXXXXXXXXXXEGEDEFMFCCARCCLGVMESIFR 415
FCFLRALTRNLGSACLGSLFVP IE EGEDEFMFCCARCCLGVM+SIFR
Sbjct: 373 FCFLRALTRNLGSACLGSLFVPTIEALRIVARGLNLLEGEDEFMFCCARCCLGVMQSIFR 432
Query: 416 NGNSWAYVQIAAYGRGFVMASQETWALFEKEDMVAVVDSDITSSICFLTGVCSGSMCVIV 475
NGNSWAYVQIAAYGRGFVMASQ+TW+LFEKEDMV +VD+DITSSICFLTGVCSGSMCVIV
Sbjct: 433 NGNSWAYVQIAAYGRGFVMASQDTWSLFEKEDMVPIVDADITSSICFLTGVCSGSMCVIV 492
Query: 476 VAAWTHTVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTIK 535
VAAWT +VHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTIK
Sbjct: 493 VAAWTQSVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTIK 552
Query: 536 DRQALLETRREMVVPTPRGVRRYT 559
DRQALL+T RE+VVPTPRG+RRYT
Sbjct: 553 DRQALLKTGREVVVPTPRGLRRYT 576
>Medtr3g033520.1 | plasma-membrane choline transporter family
protein | HC | chr3:10739614-10743112 | 20130731
Length = 560
Score = 821 bits (2120), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 401/505 (79%), Positives = 433/505 (85%), Gaps = 6/505 (1%)
Query: 61 FNLSRFNRLNPTNPLRIVINXXXXXXXXXXXXXXXXXXXHPRSIPIPTXXXXXXXXXXXX 120
FN+SRF+RLNPTNPLRIV+N RSIPIPT
Sbjct: 53 FNVSRFHRLNPTNPLRIVMNSNTRVAKPPPPKKSQRSHTPTRSIPIPTPAPIQTPQPPPP 112
Query: 121 XXX------VTLNSRKYTNRISLFLFVVHMFIAIALVGFLVFKGVQGLILESESMKRKKK 174
VTLNSR+YTNRISLF+F +H +AIALV FLVFKG+QGL+ ES S+KRK+K
Sbjct: 113 PPPQQQQQPVTLNSRRYTNRISLFIFALHQLLAIALVCFLVFKGIQGLVQESGSVKRKEK 172
Query: 175 NMLKFFLPQVEAASFMSIILAFIWQQAIRKWPTFMVHFILWFTFVMSLAAGILLICFQKP 234
+L +FLPQVEAA+FMSIILAFIWQ AIRKWPTFMVHFILWFTFVMSLAAGILLICFQKP
Sbjct: 173 RVLMYFLPQVEAATFMSIILAFIWQGAIRKWPTFMVHFILWFTFVMSLAAGILLICFQKP 232
Query: 235 PTDGVGVCFIAFAIGNGLYACWASNRIKFCCKILNLSLQPLSKFPDLNKPTYYMLGAGFL 294
PTDGVGVCFIAFAIGNGLY CW S+RIKFCCK+L+LSLQP+SKF DLN+PTYYML AGFL
Sbjct: 233 PTDGVGVCFIAFAIGNGLYGCWISHRIKFCCKVLSLSLQPMSKFSDLNRPTYYMLAAGFL 292
Query: 295 WISLWSLAVIGSLNFYFPPLVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSST 354
WISLW+LAV+G+LNFYFPPLVI+AL LSLAWTTEVMRNVVNITVSRVIALYYLRGMQSST
Sbjct: 293 WISLWTLAVVGALNFYFPPLVIIALVLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSST 352
Query: 355 QFCFLRALTRNLGSACLGSLFVPAIEXXXXXXXXXXXXEGEDEFMFCCARCCLGVMESIF 414
QFCFLRALTRNLGSACLGSLFVP IE EGEDEFMFCCARCCLGVM+SIF
Sbjct: 353 QFCFLRALTRNLGSACLGSLFVPTIEALRIVARGLNLLEGEDEFMFCCARCCLGVMQSIF 412
Query: 415 RNGNSWAYVQIAAYGRGFVMASQETWALFEKEDMVAVVDSDITSSICFLTGVCSGSMCVI 474
RNGNSWAYVQIAAYGRGFVMASQ+TW+LFEKEDMV +VD+DITSSICFLTGVCSGSMCVI
Sbjct: 413 RNGNSWAYVQIAAYGRGFVMASQDTWSLFEKEDMVPIVDADITSSICFLTGVCSGSMCVI 472
Query: 475 VVAAWTHTVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTI 534
VVAAWT +VHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTI
Sbjct: 473 VVAAWTQSVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTI 532
Query: 535 KDRQALLETRREMVVPTPRGVRRYT 559
KDRQALL+T RE+VVPTPRG+RRYT
Sbjct: 533 KDRQALLKTGREVVVPTPRGLRRYT 557
>Medtr7g086710.1 | plasma-membrane choline transporter family
protein | HC | chr7:33711509-33708521 | 20130731
Length = 456
Score = 206 bits (524), Expect = 5e-53, Method: Compositional matrix adjust.
Identities = 125/406 (30%), Positives = 202/406 (49%), Gaps = 14/406 (3%)
Query: 139 LFVVHMFIAIALVGFLV-FKGVQGLILESESMKRKKKNMLKFFLPQVEAASFMSIILAFI 197
LF + ++ + L+ LV F V GL+L+ + + + P + A++ IL +
Sbjct: 44 LFQILFYLYLVLISILVIFITVYGLVLDYSTHHFHPEK----WYPPLLASTVCGGILGLM 99
Query: 198 WQQAIRKWPTFMVHFILWFTFVMSLAAGILLICFQKPPTDGVGVCFIAFAIGNGLYACWA 257
WQ I P + W + +++ A GIL + + VG+ + A+ LY CW
Sbjct: 100 WQWIIASHPEKALRAAFWLSPLLTCAMGILFVLIGSALSLVVGIVSLISAVIQSLYGCWV 159
Query: 258 SNRIKFCCKILNLS-LQPLSKFPDLNKPTYYMLGAGFLWISLWSLAVIGSLNFY----FP 312
R + +IL +S P +K L + G ++ + G+
Sbjct: 160 GKRFVYATEILLVSKASPPAKTKRLACSLTVI---GIIYCCFLVSGIGGAKAIQNRTKLA 216
Query: 313 PLVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSSTQFCFLRALTRNLGSACLG 372
+ IL + LSL WT +V++N + +TVSRV +++ G T+ F + +GS C+G
Sbjct: 217 DICILVIILSLGWTMQVLKNAIQVTVSRVKYMHFSGGGDIDTRVAFCDTVKHLIGSVCIG 276
Query: 373 SLFVPAIEXXXXXXXXXXXXEGE-DEFMFCCARCCLGVMESIFRNGNSWAYVQIAAYGRG 431
S+ VP I GE +E MF C C +G+ + GN W +V + Y +G
Sbjct: 277 SILVPTIGLFRGFARSTSLIGGETNECMFSCVSCSMGIASLLVTKGNRWGFVHVGVYNKG 336
Query: 432 FVMASQETWALFEKEDMVAVVDSDITSSICFLTGVCSGSMCVIVVAAWTHTVHKSFTATL 491
FV AS +TW +F + + ++D D+T + CFL+GV G++C +V W+ ++K++ L
Sbjct: 337 FVQASSDTWDMFIRVGLEELIDLDLTGAFCFLSGVAVGAICSLVSGIWSLILYKNYAMEL 396
Query: 492 SLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKTIKDR 537
S+ F IGY L R+A+A P ACVS YYV YAENP++ FD I R
Sbjct: 397 SIYAFLIGYFLCRLAIAWPQACVSAYYVAYAENPQSTQFDSIIPVR 442
>Medtr5g054780.1 | plasma-membrane choline transporter family
protein | HC | chr5:22571945-22576944 | 20130731
Length = 491
Score = 119 bits (299), Expect = 5e-27, Method: Compositional matrix adjust.
Identities = 89/333 (26%), Positives = 153/333 (45%), Gaps = 44/333 (13%)
Query: 244 IAFAIGNGL---YACWASNRIKFCCKILNLSLQPLSKFPDLNKPTYYMLGAGFLWISLWS 300
+AFAIG L Y +R+ F +L +++ + P++ + Y + LW++LWS
Sbjct: 150 VAFAIGAALQFLYIISVIDRLPFTMLVLQKAVKMVWNLPEVMRVAYAFMAVMLLWMALWS 209
Query: 301 LAVIG----SLNFYFPPLVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRG------- 349
G S+ +++ L +SL WT V+ N V++ VS + + G
Sbjct: 210 FGAAGVVASSMGDGGRWWLLVVLSVSLFWTGAVLCNTVHVIVSGSLIRVSIHGGCEAASL 269
Query: 350 ----MQSSTQFCFLRALTRNLGSACLGSLFVPAIEXXXXXXXXXXXXEGEDEFMFCCARC 405
+ S Q+ ALT + GS C GSLF AI G++E + CC
Sbjct: 270 PTNSIMKSLQY----ALTTSFGSICYGSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDF 325
Query: 406 CLGVMESIFRNGNSWAYVQIAAYGRGFVMASQETWALFEKEDMVAVVDSDITSSICFL-- 463
++E++ R N +AYVQI YG+ F ++++ W LF+ + A+V D + ++ +
Sbjct: 326 LFHLVETLVRFFNKYAYVQIGVYGKSFNRSARDAWELFQSTGVEALVAYDCSGAVLLMGT 385
Query: 464 ------TGVCSGSMCVIVVAAWTHTVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCY 517
TG CSG V AW ++F + + +G +L +AM V + V+
Sbjct: 386 ILGGLITGTCSG------VWAWMKWSDRAFM--IGSTSMLMGMILVGVAMVVVESAVTSI 437
Query: 518 YVCYAENP------ENRLFDKTIKDRQALLETR 544
Y+CYAE+P ++ F++ + L+ R
Sbjct: 438 YICYAEDPLLIQRWDHEFFNQISETLHQRLQHR 470
>Medtr1g111330.1 | plasma-membrane choline transporter family
protein | HC | chr1:50302012-50297718 | 20130731
Length = 496
Score = 116 bits (291), Expect = 5e-26, Method: Compositional matrix adjust.
Identities = 77/296 (26%), Positives = 136/296 (45%), Gaps = 14/296 (4%)
Query: 244 IAFAIGNGL---YACWASNRIKFCCKILNLSLQPLSKFPDLNKPTYYMLGAGFLWISLWS 300
+AFA+G L Y +R+ F +L +++ + P++ + +Y + A LW++LWS
Sbjct: 156 VAFAVGAALQFLYVISVIDRLPFTMLVLQKAVKMVWNLPEVMRVSYAFMFAVLLWMALWS 215
Query: 301 LAVIGSLNFYFPP----LVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSSTQF 356
G + +++ +SL WT V+ N V++ VS ++ L L G + +
Sbjct: 216 FGAAGVVASNLSDGGRWWLLVVFSVSLFWTGAVLCNTVHVIVSGMVFLVLLHGGREAASI 275
Query: 357 -------CFLRALTRNLGSACLGSLFVPAIEXXXXXXXXXXXXEGEDEFMFCCARCCLGV 409
ALT + GS C GSLF AI G +E + CC +
Sbjct: 276 PANSLMKSLQYALTTSFGSICYGSLFTAAIRTLRWEIRGLRSKIGNNECLLCCVDFLFHL 335
Query: 410 MESIFRNGNSWAYVQIAAYGRGFVMASQETWALFEKEDMVAVVDSDITSSICFLTGVCSG 469
+E++ R N +AYVQIA G+ F ++++ W LF+ + A+V D + ++ + + G
Sbjct: 336 VETLVRFFNKYAYVQIAVNGKSFNNSARDAWELFQSTGVEALVAYDCSGAVLLMGTIFGG 395
Query: 470 SMCVIVVAAWTHTVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENP 525
+ W + + +G +L +AM V + V+ Y+CYAE+P
Sbjct: 396 LITGTCAGVWAWVKWSDRVIMIGSTSMLMGMVLVGLAMVVVESAVTSIYICYAEDP 451
>Medtr6g027560.1 | plasma-membrane choline transporter family
protein | HC | chr6:9543922-9545950 | 20130731
Length = 237
Score = 107 bits (268), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 72/252 (28%), Positives = 110/252 (43%), Gaps = 39/252 (15%)
Query: 295 WISLWSLAVIGSLNFYFPPLVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSST 354
WI+L + S F+ PL+ A+ + L + + I+L G+
Sbjct: 23 WITLKNPKKALSAIFWLSPLLTCAMAIMLVY------------IESPISLIIGCGVMMDP 70
Query: 355 QFCFLRALTRNLGSACLGSLFVPAIEXXXXXXXXXXXXEGE-DEFMFCCARCCLGVMESI 413
L +GS +GS+ VP I G+ DEFMF C C +G+ +
Sbjct: 71 SVALNDTLKYFIGSVSIGSILVPFISTFRGFARSIKIAGGDSDEFMFSCVSCYMGIASIL 130
Query: 414 FRNGNSWAYVQIAAYGRGFVMASQETWALFEKEDMVAVVDSDITSSICFLTGVCSGSMCV 473
GN + +V ++D D+T S CF +GV G++
Sbjct: 131 VSCGN--------------------------RVGLVQLIDLDLTGSFCFFSGVAGGAISS 164
Query: 474 IVVAAWTHTVHKSFTATLSLLTFFIGYLLTRIAMAVPHACVSCYYVCYAENPENRLFDKT 533
+V W+ + K++ LS+ F IGY + R+A+A P ACVS YYV YAENP++ FD T
Sbjct: 165 LVSGIWSIVLDKNYATELSIYGFLIGYFMVRLALACPQACVSAYYVAYAENPQSTHFDST 224
Query: 534 IKDRQALLETRR 545
I R L+ +
Sbjct: 225 IPMRLEQLQRSQ 236
>Medtr1g111330.2 | plasma-membrane choline transporter family
protein | HC | chr1:50302008-50297730 | 20130731
Length = 354
Score = 73.9 bits (180), Expect = 3e-13, Method: Compositional matrix adjust.
Identities = 53/196 (27%), Positives = 90/196 (45%), Gaps = 14/196 (7%)
Query: 244 IAFAIGNGL---YACWASNRIKFCCKILNLSLQPLSKFPDLNKPTYYMLGAGFLWISLWS 300
+AFA+G L Y +R+ F +L +++ + P++ + +Y + A LW++LWS
Sbjct: 156 VAFAVGAALQFLYVISVIDRLPFTMLVLQKAVKMVWNLPEVMRVSYAFMFAVLLWMALWS 215
Query: 301 LAVIGSLNFYFPP----LVILALGLSLAWTTEVMRNVVNITVSRVIALYYLRGMQSSTQF 356
G + +++ +SL WT V+ N V++ VS ++ L L G + +
Sbjct: 216 FGAAGVVASNLSDGGRWWLLVVFSVSLFWTGAVLCNTVHVIVSGMVFLVLLHGGREAASI 275
Query: 357 -------CFLRALTRNLGSACLGSLFVPAIEXXXXXXXXXXXXEGEDEFMFCCARCCLGV 409
ALT + GS C GSLF AI G +E + CC +
Sbjct: 276 PANSLMKSLQYALTTSFGSICYGSLFTAAIRTLRWEIRGLRSKIGNNECLLCCVDFLFHL 335
Query: 410 MESIFRNGNSWAYVQI 425
+E++ R N +AYVQ+
Sbjct: 336 VETLVRFFNKYAYVQV 351