Miyakogusa Predicted Gene

Lj3g3v0428550.3
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v0428550.3 tr|I3T024|I3T024_LOTJA Malate dehydrogenase
OS=Lotus japonicus PE=2 SV=1,98.24,0,MDH,Malate dehydrogenase, active
site; MDH_euk_gproteo: malate dehydrogenase, NAD-depende,Malate
deh,CUFF.40823.3
         (341 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g463760.1 | malate dehydrogenase | HC | chr8:22494348-2249...   578   e-165
Medtr5g014710.1 | glyoxysomal malate dehydrogenase | HC | chr5:4...   414   e-116
Medtr4g092690.1 | glyoxysomal malate dehydrogenase | HC | chr4:3...   394   e-110
Medtr8g463760.2 | malate dehydrogenase | HC | chr8:22494347-2249...   362   e-100
Medtr5g012390.1 | L-lactate dehydrogenase A-like protein | HC | ...    54   2e-07
Medtr2g045010.1 | malate dehydrogenase | HC | chr2:19586812-1958...    52   8e-07
Medtr2g021700.1 | malate dehydrogenase | HC | chr2:7390300-73922...    52   8e-07

>Medtr8g463760.1 | malate dehydrogenase | HC |
           chr8:22494348-22490385 | 20130731
          Length = 343

 Score =  578 bits (1491), Expect = e-165,   Method: Compositional matrix adjust.
 Identities = 283/343 (82%), Positives = 311/343 (90%), Gaps = 2/343 (0%)

Query: 1   MMKPSMLRSAVSRCSQ-LSRRGYATNPVPERKVAVLGAAGGIGQPLSLLMKLNPLVSSLS 59
           MM+PS+LRS  S  S+ ++RRGYAT PVPERKVA+LGAAGGIGQPLSLLMKLNPLVS+LS
Sbjct: 1   MMRPSILRSVQSAVSRSITRRGYATEPVPERKVAILGAAGGIGQPLSLLMKLNPLVSTLS 60

Query: 60  LYDIAGTPGVAADVSHINTRSEVVGYQGEEQLGKALEGADIVIIPAGVPRKPGMTRDDLF 119
           LYDIAGTPGVAADVSHIN+RS+V GY GE++LGKALEGAD+VIIPAGVPRKPGMTRDDLF
Sbjct: 61  LYDIAGTPGVAADVSHINSRSQVTGYAGEDELGKALEGADVVIIPAGVPRKPGMTRDDLF 120

Query: 120 NINAGIVKGLCSAIAKYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDERRLFGVTTLDV 179
           NINAGIVK L +AI+KYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDE+RLFGVTTLDV
Sbjct: 121 NINAGIVKSLATAISKYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDEKRLFGVTTLDV 180

Query: 180 VRAKTFYAGXXXXXXXXXXXXXXGGHAGITILPLFSQATPQANLDHDLIQALTKRTQDGG 239
           VRAKTFYAG              GGHAG+TILPLFSQA+PQANLD D+I+ALT RTQDGG
Sbjct: 181 VRAKTFYAGKANVPVAEVNVPVIGGHAGVTILPLFSQASPQANLDDDVIKALTARTQDGG 240

Query: 240 TEVVEAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVVECSYVQSS-VTDLPFFASKVR 298
           TEVV AKAGKGSATLSMAYAGAIFADACLKGLNGVPDVVECSYVQS+ + +LPFFASKVR
Sbjct: 241 TEVVTAKAGKGSATLSMAYAGAIFADACLKGLNGVPDVVECSYVQSNLIAELPFFASKVR 300

Query: 299 LGKNGWDEVLGLGTLSDFENQGLENLKGELQSSIEKGIKFANQ 341
           +GKNG +E+LGLG+LSDFE QGLENLK EL+SSIEKGIKFA+Q
Sbjct: 301 IGKNGVEEILGLGSLSDFEKQGLENLKSELKSSIEKGIKFASQ 343


>Medtr5g014710.1 | glyoxysomal malate dehydrogenase | HC |
           chr5:4992016-4987668 | 20130731
          Length = 358

 Score =  414 bits (1064), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 206/311 (66%), Positives = 237/311 (76%)

Query: 28  PERKVAVLGAAGGIGQPLSLLMKLNPLVSSLSLYDIAGTPGVAADVSHINTRSEVVGYQG 87
           P  KVA+LGAAGGIGQPLS+LMK+NPLVS L LYD+  TPGV +D+SH++T + V G+ G
Sbjct: 45  PGFKVAILGAAGGIGQPLSMLMKINPLVSVLHLYDVVNTPGVTSDISHMDTSAVVRGFLG 104

Query: 88  EEQLGKALEGADIVIIPAGVPRKPGMTRDDLFNINAGIVKGLCSAIAKYCPHALVNMISN 147
           + QL  AL G D+VIIPAGVPRKPGMTRDDLFNINAGIVK LC AIAK CP A+VN+ISN
Sbjct: 105 QNQLEDALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIVKTLCEAIAKQCPKAIVNLISN 164

Query: 148 PVNSTVPIAAEVFKKAGTYDERRLFGVTTLDVVRAKTFYAGXXXXXXXXXXXXXXGGHAG 207
           PVNSTVPIAAEVFK+AGTYD +RL GVT LDVVRA TF A               GGHAG
Sbjct: 165 PVNSTVPIAAEVFKRAGTYDPKRLLGVTMLDVVRANTFVAEVMGLDPRDVDVPVVGGHAG 224

Query: 208 ITILPLFSQATPQANLDHDLIQALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADAC 267
           ITILPL SQ  P ++     I+ LT R Q+GGTEVVEAKAG GSATLSMAYA   FADAC
Sbjct: 225 ITILPLLSQVKPPSSFTPKEIEYLTDRIQNGGTEVVEAKAGAGSATLSMAYAAVKFADAC 284

Query: 268 LKGLNGVPDVVECSYVQSSVTDLPFFASKVRLGKNGWDEVLGLGTLSDFENQGLENLKGE 327
           L+ L G  D+V+C+YV S VT+LPFFASKVRLG+NG +E L LG LSD+E   LE  K E
Sbjct: 285 LRALKGEADIVQCAYVDSQVTELPFFASKVRLGRNGVEEFLPLGPLSDYERASLEKAKKE 344

Query: 328 LQSSIEKGIKF 338
           L +S+EKG+ F
Sbjct: 345 LATSVEKGVSF 355


>Medtr4g092690.1 | glyoxysomal malate dehydrogenase | HC |
           chr4:36762718-36765660 | 20130731
          Length = 356

 Score =  394 bits (1012), Expect = e-110,   Method: Compositional matrix adjust.
 Identities = 199/311 (63%), Positives = 235/311 (75%)

Query: 28  PERKVAVLGAAGGIGQPLSLLMKLNPLVSSLSLYDIAGTPGVAADVSHINTRSEVVGYQG 87
           P  KVAVLGAAGGIGQ LSLL+K+NPLVS L LYD+  TPGV ADVSH++T + V G+ G
Sbjct: 43  PGFKVAVLGAAGGIGQSLSLLLKMNPLVSVLHLYDVVNTPGVTADVSHMDTGAVVRGFLG 102

Query: 88  EEQLGKALEGADIVIIPAGVPRKPGMTRDDLFNINAGIVKGLCSAIAKYCPHALVNMISN 147
           + QL  AL G D+V+IPAGVPRKPGMTRDDLF INAGIV+ LC  IAK CP+A+VN+ISN
Sbjct: 103 QPQLENALTGMDLVVIPAGVPRKPGMTRDDLFKINAGIVRTLCEGIAKCCPNAIVNLISN 162

Query: 148 PVNSTVPIAAEVFKKAGTYDERRLFGVTTLDVVRAKTFYAGXXXXXXXXXXXXXXGGHAG 207
           PVNSTVPIAAEVFKKAGTYD +RL GVT LDVVRA TF A               GGHAG
Sbjct: 163 PVNSTVPIAAEVFKKAGTYDPKRLLGVTALDVVRANTFVAEVLGVDPRDVDVPVVGGHAG 222

Query: 208 ITILPLFSQATPQANLDHDLIQALTKRTQDGGTEVVEAKAGKGSATLSMAYAGAIFADAC 267
           +TILPL SQ  P ++   +  + LT R Q+GGTEVVEAKAG GSATLSMAYA A FA++C
Sbjct: 223 VTILPLLSQVKPPSSFTAEETEYLTNRIQNGGTEVVEAKAGAGSATLSMAYAAAKFANSC 282

Query: 268 LKGLNGVPDVVECSYVQSSVTDLPFFASKVRLGKNGWDEVLGLGTLSDFENQGLENLKGE 327
           L GL G   V+EC+YV S VT+LPFFA+KVRLG+ G +E+  LG L+++E  GLE  K E
Sbjct: 283 LHGLKGEAGVIECAYVDSQVTELPFFATKVRLGRAGAEEIFPLGPLNEYERIGLEKAKRE 342

Query: 328 LQSSIEKGIKF 338
           L  SI+KG++F
Sbjct: 343 LAGSIQKGVEF 353


>Medtr8g463760.2 | malate dehydrogenase | HC |
           chr8:22494347-22490385 | 20130731
          Length = 251

 Score =  362 bits (928), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 177/217 (81%), Positives = 193/217 (88%), Gaps = 1/217 (0%)

Query: 1   MMKPSMLRSAVSRCSQ-LSRRGYATNPVPERKVAVLGAAGGIGQPLSLLMKLNPLVSSLS 59
           MM+PS+LRS  S  S+ ++RRGYAT PVPERKVA+LGAAGGIGQPLSLLMKLNPLVS+LS
Sbjct: 1   MMRPSILRSVQSAVSRSITRRGYATEPVPERKVAILGAAGGIGQPLSLLMKLNPLVSTLS 60

Query: 60  LYDIAGTPGVAADVSHINTRSEVVGYQGEEQLGKALEGADIVIIPAGVPRKPGMTRDDLF 119
           LYDIAGTPGVAADVSHIN+RS+V GY GE++LGKALEGAD+VIIPAGVPRKPGMTRDDLF
Sbjct: 61  LYDIAGTPGVAADVSHINSRSQVTGYAGEDELGKALEGADVVIIPAGVPRKPGMTRDDLF 120

Query: 120 NINAGIVKGLCSAIAKYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDERRLFGVTTLDV 179
           NINAGIVK L +AI+KYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDE+RLFGVTTLDV
Sbjct: 121 NINAGIVKSLATAISKYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDEKRLFGVTTLDV 180

Query: 180 VRAKTFYAGXXXXXXXXXXXXXXGGHAGITILPLFSQ 216
           VRAKTFYAG              GGHAG+TILPLFSQ
Sbjct: 181 VRAKTFYAGKANVPVAEVNVPVIGGHAGVTILPLFSQ 217


>Medtr5g012390.1 | L-lactate dehydrogenase A-like protein | HC |
           chr5:3706531-3705145 | 20130731
          Length = 350

 Score = 53.9 bits (128), Expect = 2e-07,   Method: Compositional matrix adjust.
 Identities = 60/268 (22%), Positives = 108/268 (40%), Gaps = 32/268 (11%)

Query: 22  YATNPVPER---KVAVLGAAGGIGQPLSLLMKLNPLVSSLSLYDIAGTP----GVAADVS 74
           +A+ P P +   K++V+GA G +G  ++  +    LV  L L D    P    G   D+ 
Sbjct: 27  HASPPSPTKRHNKISVIGA-GNVGMAIAQTILTQDLVDELVLVD--AIPDKLRGEMLDLQ 83

Query: 75  HINTRSEVVGYQGEEQLGKALEGADIVIIPAGVPRKPGMTRDDLFNINAGIVKGLCSAIA 134
           H          Q        + G+D+ I+ AG  +  G +R +L   N  + K +   + 
Sbjct: 84  HAAAFLPRTKIQASTDYSVTM-GSDLCIVTAGARQINGESRLNLLQRNVALFKKIIPPLV 142

Query: 135 KYCPHALVNMISNPVNSTVPIAAEVFKKAGTYDERRLFGVTTLDVVRAKTFYAGXXXXXX 194
           +Y PH ++ ++SNPV+    IA   +K +G    R +   T LD  R +   A       
Sbjct: 143 RYSPHCVLLIVSNPVDVLTYIA---WKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNA 199

Query: 195 XXXXXXXXGGH-------------AGITILPLFSQATPQANLDHDLIQALTKRTQDGGTE 241
                   G H              G+ +L    +   Q   + ++++ + K   +G  E
Sbjct: 200 QDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQ--QIAYEKEMLENIHKEVINGAYE 257

Query: 242 VVEAKAGKGSATLSMAYAGAIFADACLK 269
           V+     KG  + ++ Y+ A  A   ++
Sbjct: 258 VISL---KGYTSWAIGYSVANLARTIMR 282


>Medtr2g045010.1 | malate dehydrogenase | HC |
           chr2:19586812-19584816 | 20130731
          Length = 150

 Score = 52.0 bits (123), Expect = 8e-07,   Method: Compositional matrix adjust.
 Identities = 24/44 (54%), Positives = 32/44 (72%)

Query: 203 GGHAGITILPLFSQATPQANLDHDLIQALTKRTQDGGTEVVEAK 246
           GGHAG+TILPL SQA   ++   +  + LT R Q+GGTEVVE++
Sbjct: 59  GGHAGVTILPLLSQAKSPSSFTAEETEYLTNRIQNGGTEVVESQ 102


>Medtr2g021700.1 | malate dehydrogenase | HC | chr2:7390300-7392293
           | 20130731
          Length = 150

 Score = 52.0 bits (123), Expect = 8e-07,   Method: Compositional matrix adjust.
 Identities = 24/44 (54%), Positives = 32/44 (72%)

Query: 203 GGHAGITILPLFSQATPQANLDHDLIQALTKRTQDGGTEVVEAK 246
           GGHAG+TILPL SQA   ++   +  + LT R Q+GGTEVVE++
Sbjct: 59  GGHAGVTILPLLSQAKSPSSFTAEETEYLTNRIQNGGTEVVESQ 102