Miyakogusa Predicted Gene

Lj3g3v0424170.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj3g3v0424170.1 Non Characterized Hit- tr|I1MEN1|I1MEN1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.57429
PE,81.67,0,S-adenosyl-L-methionine-dependent methyltransferases,NULL;
GCD14,tRNA (adenine(58)-N(1))-methyltrans,CUFF.40678.1
         (309 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr6g047800.2 | tRNA methyltransferase complex GCD14 subunit |...   491   e-139
Medtr6g047800.1 | tRNA methyltransferase complex GCD14 subunit |...   483   e-136
Medtr2g014590.1 | tRNA methyltransferase complex GCD14 subunit |...   358   3e-99
Medtr2g014570.1 | tRNA methyltransferase complex GCD14 subunit |...    86   3e-17

>Medtr6g047800.2 | tRNA methyltransferase complex GCD14 subunit | HC
           | chr6:17240555-17244407 | 20130731
          Length = 309

 Score =  491 bits (1265), Expect = e-139,   Method: Compositional matrix adjust.
 Identities = 245/309 (79%), Positives = 266/309 (86%), Gaps = 3/309 (0%)

Query: 1   MLASESAKRISFNRSISNGDLVIVYERHDSMKAVTVTEGSVLQNRFGVFKHSDWIGKSFG 60
           MLA +S +++SFNR+ISNGDL IVY RHD+MK VTV+EGSVLQNRFGVFKHSDWIGKSFG
Sbjct: 1   MLAIDSTRKMSFNRTISNGDLAIVYARHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFG 60

Query: 61  SKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGCLVXXXXXXXX 120
           SKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPG +V        
Sbjct: 61  SKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGFVVLESGTGSG 120

Query: 121 XXXXXXARAVAPTGHVYTFDFHEQRAGSARDDFERTGLSKLVTVGVRDIQGEGFPEEFAG 180
                 ARAVAPTGHVYTFDFHEQR+GSARDDFE+ G+S LVT GVRDIQGEGFPEEFAG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAG 180

Query: 181 SVDAVFLDLPQPWLAIPSAAKMLKQDATLCSFSPCIEQVQRSCETLRTSFTDIRTFEVLL 240
             DAVFLDLPQPWLAIPSAAKML+ D TLCSFSPCIEQVQRSCET++++FTDIRTFEVLL
Sbjct: 181 LADAVFLDLPQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLL 240

Query: 241 RTYEVRETKMERLQENGNS-SNVSL-PCKRRQCSDGGNVLSSSISSVT-ARPCGEARGHT 297
           RTYEVRE KME L+ +G+  SN SL PCKRRQ SDG +V+S   SS   ARPCGEARGHT
Sbjct: 241 RTYEVREGKMESLEIDGDDGSNGSLPPCKRRQRSDGASVVSYPSSSSVMARPCGEARGHT 300

Query: 298 GYLTFARLK 306
           GYLTFARL 
Sbjct: 301 GYLTFARLN 309


>Medtr6g047800.1 | tRNA methyltransferase complex GCD14 subunit | HC
           | chr6:17241702-17244129 | 20130731
          Length = 300

 Score =  483 bits (1242), Expect = e-136,   Method: Compositional matrix adjust.
 Identities = 241/300 (80%), Positives = 259/300 (86%), Gaps = 3/300 (1%)

Query: 10  ISFNRSISNGDLVIVYERHDSMKAVTVTEGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGG 69
           +SFNR+ISNGDL IVY RHD+MK VTV+EGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGG
Sbjct: 1   MSFNRTISNGDLAIVYARHDNMKPVTVSEGSVLQNRFGVFKHSDWIGKSFGSKVFSNKGG 60

Query: 70  FVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGCLVXXXXXXXXXXXXXXARA 129
           FVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPG +V              ARA
Sbjct: 61  FVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGFVVLESGTGSGSLTTSLARA 120

Query: 130 VAPTGHVYTFDFHEQRAGSARDDFERTGLSKLVTVGVRDIQGEGFPEEFAGSVDAVFLDL 189
           VAPTGHVYTFDFHEQR+GSARDDFE+ G+S LVT GVRDIQGEGFPEEFAG  DAVFLDL
Sbjct: 121 VAPTGHVYTFDFHEQRSGSARDDFEKIGISSLVTAGVRDIQGEGFPEEFAGLADAVFLDL 180

Query: 190 PQPWLAIPSAAKMLKQDATLCSFSPCIEQVQRSCETLRTSFTDIRTFEVLLRTYEVRETK 249
           PQPWLAIPSAAKML+ D TLCSFSPCIEQVQRSCET++++FTDIRTFEVLLRTYEVRE K
Sbjct: 181 PQPWLAIPSAAKMLRHDGTLCSFSPCIEQVQRSCETMQSNFTDIRTFEVLLRTYEVREGK 240

Query: 250 MERLQENG-NSSNVSL-PCKRRQCSDGGNVLSSSISSVT-ARPCGEARGHTGYLTFARLK 306
           ME L+ +G + SN SL PCKRRQ SDG +V+S   SS   ARPCGEARGHTGYLTFARL 
Sbjct: 241 MESLEIDGDDGSNGSLPPCKRRQRSDGASVVSYPSSSSVMARPCGEARGHTGYLTFARLN 300


>Medtr2g014590.1 | tRNA methyltransferase complex GCD14 subunit | HC
           | chr2:4205476-4203960 | 20130731
          Length = 237

 Score =  358 bits (920), Expect = 3e-99,   Method: Compositional matrix adjust.
 Identities = 168/231 (72%), Positives = 186/231 (80%)

Query: 1   MLASESAKRISFNRSISNGDLVIVYERHDSMKAVTVTEGSVLQNRFGVFKHSDWIGKSFG 60
           +L S   K I+FNR I +G+LVIVY +H +MKAVTV+EGSV QN  G FKHSDWIGK FG
Sbjct: 2   LLTSNPEKTIAFNRKIHDGNLVIVYVKHGNMKAVTVSEGSVFQNSLGAFKHSDWIGKPFG 61

Query: 61  SKVFSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEIVPGCLVXXXXXXXX 120
           SKVF+NKG FVYLLAPTPELWTLVL+HRTQILYI DISFV+MYLEIV GCLV        
Sbjct: 62  SKVFNNKGDFVYLLAPTPELWTLVLNHRTQILYIPDISFVIMYLEIVAGCLVLESGTGSG 121

Query: 121 XXXXXXARAVAPTGHVYTFDFHEQRAGSARDDFERTGLSKLVTVGVRDIQGEGFPEEFAG 180
                 ARA+AP GHVYTFDFHEQRA SARDDFER GLS L++ GVRDIQGEGFP+E   
Sbjct: 122 SLTTSLARAIAPNGHVYTFDFHEQRAASARDDFERIGLSSLISCGVRDIQGEGFPDELNE 181

Query: 181 SVDAVFLDLPQPWLAIPSAAKMLKQDATLCSFSPCIEQVQRSCETLRTSFT 231
             D+VFLDLPQPWLAIPSAAKMLKQD T CSFSPCIEQVQRSC+ L++ FT
Sbjct: 182 LADSVFLDLPQPWLAIPSAAKMLKQDGTFCSFSPCIEQVQRSCDALQSCFT 232


>Medtr2g014570.1 | tRNA methyltransferase complex GCD14 subunit | HC
           | chr2:4202155-4201903 | 20130731
          Length = 66

 Score = 86.3 bits (212), Expect = 3e-17,   Method: Compositional matrix adjust.
 Identities = 43/64 (67%), Positives = 46/64 (71%), Gaps = 3/64 (4%)

Query: 246 RETKMERLQENGNSSNVSLPCKRRQCSDGGNVLSSSISSVTARPCGEARGHTGYLTFARL 305
           RE K +    + N S   LPCKRRQCSDG  VLS+  SSV ARPC EARGHTGYLTFARL
Sbjct: 6   REVKTKSFHGDDNGS---LPCKRRQCSDGNYVLSNPTSSVMARPCDEARGHTGYLTFARL 62

Query: 306 KCLS 309
           KC S
Sbjct: 63  KCFS 66