Miyakogusa Predicted Gene

Lj2g3v3281380.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v3281380.1 Non Characterized Hit- tr|I3S745|I3S745_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.71,0,EF_HAND_1,EF-Hand 1, calcium-binding site;
2POREKCHANEL,Two pore domain potassium channel; Ion_trans,CUFF.39949.1
         (349 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g095650.2 | outward rectifying potassium channel protein |...   508   e-144
Medtr5g095650.1 | outward rectifying potassium channel protein |...   508   e-144
Medtr4g133870.1 | outward rectifying potassium channel protein |...   342   2e-94
Medtr5g091565.1 | outward rectifying potassium channel protein |...   190   2e-48
Medtr4g094582.1 | outward rectifying potassium channel protein |...   188   6e-48
Medtr7g110600.1 | outward rectifying potassium channel protein |...   187   1e-47
Medtr4g133820.1 | hypothetical protein | LC | chr4:55973846-5597...    54   2e-07

>Medtr5g095650.2 | outward rectifying potassium channel protein | HC
           | chr5:41807536-41811170 | 20130731
          Length = 349

 Score =  508 bits (1308), Expect = e-144,   Method: Compositional matrix adjust.
 Identities = 250/349 (71%), Positives = 281/349 (80%)

Query: 1   MANNDSQEPLISGSMDPSAQKARLQLKKRRLIRSRSAPQTDLDPPETNGNKLIPLSGSIF 60
           MA++D+ EPL+  S D  AQKA+ +L +RRL+RSRSAP TD  P   N  + IPLS +IF
Sbjct: 1   MASDDTNEPLLLRSQDTVAQKAKGRLNQRRLLRSRSAPHTDHAPLVINDKESIPLSETIF 60

Query: 61  GSLHPSFRKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLV 120
           G+LHPSF+KVA+ L VY+GVG L FYLVRNQIKG+KT RFLDALYFTIVTMTTVGYGDLV
Sbjct: 61  GNLHPSFKKVAIILMVYLGVGTLIFYLVRNQIKGMKTERFLDALYFTIVTMTTVGYGDLV 120

Query: 121 PNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSEILKEIE 180
           PNS+LTKLLACAFVFSGMA++GLILSKAADYLVEKQE LL+KAMHMR+  GPSEILKE+E
Sbjct: 121 PNSDLTKLLACAFVFSGMALMGLILSKAADYLVEKQEVLLIKAMHMRQKVGPSEILKELE 180

Query: 181 TNKTRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAG 240
            NKTRYK                    +VEKL+ +DAFYCVCSTITTLGYGDKSFSTQAG
Sbjct: 181 INKTRYKFFLVFLLLLILVIVGTIFLVNVEKLEVIDAFYCVCSTITTLGYGDKSFSTQAG 240

Query: 241 RVFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVG 300
           R+FAV WIL GTI +AQFFLYMAELNTESRQK L  WVL RK+T+           GTVG
Sbjct: 241 RIFAVFWILTGTICLAQFFLYMAELNTESRQKALVNWVLTRKMTNYDLEAADLDEDGTVG 300

Query: 301 ADEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDITLAQSS 349
           A EFVIYKLKEMGKISQEDI+L +KEFEELD+DQSGTLSVSDITLAQSS
Sbjct: 301 AAEFVIYKLKEMGKISQEDITLVMKEFEELDIDQSGTLSVSDITLAQSS 349


>Medtr5g095650.1 | outward rectifying potassium channel protein | HC
           | chr5:41807536-41811156 | 20130731
          Length = 349

 Score =  508 bits (1308), Expect = e-144,   Method: Compositional matrix adjust.
 Identities = 250/349 (71%), Positives = 281/349 (80%)

Query: 1   MANNDSQEPLISGSMDPSAQKARLQLKKRRLIRSRSAPQTDLDPPETNGNKLIPLSGSIF 60
           MA++D+ EPL+  S D  AQKA+ +L +RRL+RSRSAP TD  P   N  + IPLS +IF
Sbjct: 1   MASDDTNEPLLLRSQDTVAQKAKGRLNQRRLLRSRSAPHTDHAPLVINDKESIPLSETIF 60

Query: 61  GSLHPSFRKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLV 120
           G+LHPSF+KVA+ L VY+GVG L FYLVRNQIKG+KT RFLDALYFTIVTMTTVGYGDLV
Sbjct: 61  GNLHPSFKKVAIILMVYLGVGTLIFYLVRNQIKGMKTERFLDALYFTIVTMTTVGYGDLV 120

Query: 121 PNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSEILKEIE 180
           PNS+LTKLLACAFVFSGMA++GLILSKAADYLVEKQE LL+KAMHMR+  GPSEILKE+E
Sbjct: 121 PNSDLTKLLACAFVFSGMALMGLILSKAADYLVEKQEVLLIKAMHMRQKVGPSEILKELE 180

Query: 181 TNKTRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAG 240
            NKTRYK                    +VEKL+ +DAFYCVCSTITTLGYGDKSFSTQAG
Sbjct: 181 INKTRYKFFLVFLLLLILVIVGTIFLVNVEKLEVIDAFYCVCSTITTLGYGDKSFSTQAG 240

Query: 241 RVFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVG 300
           R+FAV WIL GTI +AQFFLYMAELNTESRQK L  WVL RK+T+           GTVG
Sbjct: 241 RIFAVFWILTGTICLAQFFLYMAELNTESRQKALVNWVLTRKMTNYDLEAADLDEDGTVG 300

Query: 301 ADEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDITLAQSS 349
           A EFVIYKLKEMGKISQEDI+L +KEFEELD+DQSGTLSVSDITLAQSS
Sbjct: 301 AAEFVIYKLKEMGKISQEDITLVMKEFEELDIDQSGTLSVSDITLAQSS 349


>Medtr4g133870.1 | outward rectifying potassium channel protein | HC
           | chr4:55993276-55996041 | 20130731
          Length = 353

 Score =  342 bits (878), Expect = 2e-94,   Method: Compositional matrix adjust.
 Identities = 182/347 (52%), Positives = 225/347 (64%), Gaps = 4/347 (1%)

Query: 1   MANNDSQEPLISGSMDPSAQKARLQLKKRRLIRSRSAPQTDLD-PPETNGNKLIPLSGSI 59
           M ++++Q+ L+S S + S    +  L +RR  R     +TD++   E NG    PLS   
Sbjct: 1   MGSDETQQLLLSESREHSLLNEKNDLLQRRKPRCGGTSETDINLQQEQNGVLQSPLSPQC 60

Query: 60  FGSLHPS---FRKVALCLAVYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGY 116
                 +   FR V LCLA Y+G G L FYL   QI+G+KTN FLDALYF +VTMTTVGY
Sbjct: 61  ITEKQEAEFKFRLVFLCLAAYLGTGTLCFYLTSYQIEGIKTNGFLDALYFCVVTMTTVGY 120

Query: 117 GDLVPNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSEIL 176
           GDLVPNS + KLLAC +VF+GMA+ GLILSKAADY+VEKQE  L ++M   ENFG  E+ 
Sbjct: 121 GDLVPNSTIAKLLACIYVFTGMALGGLILSKAADYIVEKQEIFLAESMCKAENFGLQEVA 180

Query: 177 KEIETNKTRYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFS 236
           KE+ T K++YK                     +E LDFVDA YCVCST+TTLGYGDKSFS
Sbjct: 181 KELGTKKSKYKFVLAVATFFVLMIAGTVFLYFIENLDFVDALYCVCSTVTTLGYGDKSFS 240

Query: 237 TQAGRVFAVIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXX 296
           T AGR+FAV WIL  TI +AQ F Y+AEL TE RQ+ LAK VL RK++            
Sbjct: 241 TAAGRIFAVFWILSSTICLAQSFAYLAELYTEDRQRSLAKMVLTRKLSLSDLEAADLDGD 300

Query: 297 GTVGADEFVIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDI 343
             V A EFV+YKLKEMGKI+QEDIS  ++ F +LD DQSGTL+ +DI
Sbjct: 301 KAVSAAEFVVYKLKEMGKINQEDISAVMESFRKLDCDQSGTLTEADI 347


>Medtr5g091565.1 | outward rectifying potassium channel protein | HC
           | chr5:39924920-39923156 | 20130731
          Length = 383

 Score =  190 bits (483), Expect = 2e-48,   Method: Compositional matrix adjust.
 Identities = 110/339 (32%), Positives = 180/339 (53%), Gaps = 34/339 (10%)

Query: 22  ARLQLKKRRLI-RSRSAPQT-------DLDPPETNGNKLIPLSGSIFGSLHPSFRKVALC 73
           A L +KK ++I RSRSAP         D   P  +G    P   SI          V LC
Sbjct: 58  ANLSIKKAKIIHRSRSAPSVLFTNMGVDFHEPSDHG----PAQNSII---------VRLC 104

Query: 74  LAV---YVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTKLLA 130
             +   YV +G +  Y++    KG  T R +DA+YFT+VT+ T+GYGD+VP++  TK+  
Sbjct: 105 FIIVFLYVAIG-VTVYMISGSFKGTTTFRPVDAVYFTVVTLCTIGYGDIVPDTIFTKMFT 163

Query: 131 CAFVFSGMAVVGLILSKAADYLVEKQEALLVKAMHMRENFGPSEILKEIETN------KT 184
           C F+  G  ++G +L++   ++ +  EA L+  M   +     +IL+    +      + 
Sbjct: 164 CGFILVGFGLIGFMLNELVVHICDTHEAFLLSMMGGDKY---KKILRTYMVDEKKGRMRI 220

Query: 185 RYKXXXXXXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRVFA 244
           R K                     VE L++ D+FY   +++TT+GYGD SF T  GR FA
Sbjct: 221 RTKVCVALVVVIVCIAIGTVTAHFVENLNWADSFYLSITSVTTVGYGDYSFRTLTGRCFA 280

Query: 245 VIWILIGTITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGADEF 304
           ++W+L+ T +V++ F+Y+ + + + R +++AK VL++KIT            G++   +F
Sbjct: 281 ILWLLVSTFSVSRAFIYLTDYSMQKRSRKMAKMVLQKKITLSDLAAADLDNDGSISKSDF 340

Query: 305 VIYKLKEMGKISQEDISLFLKEFEELDVDQSGTLSVSDI 343
           VIYKLK+MGKI++ DI    K+F+ LD    G ++++D+
Sbjct: 341 VIYKLKQMGKITEMDIMQISKQFDSLDHGMYGKIALADL 379


>Medtr4g094582.1 | outward rectifying potassium channel protein | HC
           | chr4:38278110-38286225 | 20130731
          Length = 418

 Score =  188 bits (478), Expect = 6e-48,   Method: Compositional matrix adjust.
 Identities = 104/324 (32%), Positives = 170/324 (52%), Gaps = 6/324 (1%)

Query: 27  KKRRLIRSRSAPQTDLDPPETNGNKLIPLSGSIFGSLHPSFRKVALCLAVYVGVGALAFY 86
           +K  L RSR+AP   +    ++ N         FGS      +  + L +Y+ +G + ++
Sbjct: 99  RKTNLHRSRTAPAMAV---ISDFNHHASDKRPQFGS-QSIVHQAVILLVLYLALGVVIYW 154

Query: 87  LVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTKLLACAFVFSGMAVVGLILS 146
             R+     +T+  +DALYF IVTM T+GYGD+ PNS  TK+ +  FV  G   + ++LS
Sbjct: 155 FNRHNFTATETHPIVDALYFCIVTMCTIGYGDITPNSTATKIFSILFVLIGFGFIDILLS 214

Query: 147 KAADYLVEKQEALLVKAMHMRENFGPSEILKEIETNKTR--YKXXXXXXXXXXXXXXXXX 204
               Y+++ QE  L++ +  R        + +++  + R   K                 
Sbjct: 215 GMVSYVLDLQENHLLRTVKGRGAKDGRSYIVDVKKGRMRIRMKVALALGVVVLCIGLGVT 274

Query: 205 XXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRVFAVIWILIGTITVAQFFLYMAE 264
               VE+L +VD+FY    ++TT+GYGD +F T  GR+FA IW+L+ T+ VA+ FLY+AE
Sbjct: 275 VMHFVERLGWVDSFYLSVMSVTTVGYGDHAFKTLHGRIFAAIWLLVSTLAVARAFLYLAE 334

Query: 265 LNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGADEFVIYKLKEMGKISQEDISLFL 324
              + R + +AKW+L + +T            G V   E+VIYKLKEMGK++++DI    
Sbjct: 335 ARVDKRHRRMAKWILGQDMTVAEFLAADMDNNGFVSKSEYVIYKLKEMGKVTEKDIMQIS 394

Query: 325 KEFEELDVDQSGTLSVSDITLAQS 348
           ++F+ LD    G +++ D+    S
Sbjct: 395 EKFDRLDTGNCGKITLGDLMEGHS 418


>Medtr7g110600.1 | outward rectifying potassium channel protein | HC
           | chr7:45328955-45326338 | 20130731
          Length = 382

 Score =  187 bits (475), Expect = 1e-47,   Method: Compositional matrix adjust.
 Identities = 109/337 (32%), Positives = 176/337 (52%), Gaps = 15/337 (4%)

Query: 20  QKARLQLKKRRLIRSRSAPQTDLDP---PETNGNKLI-PLSGSIFGSLHPSFRKVALCLA 75
           Q++    K+++L R ++AP   + P   P  +  +L  P + SI        R+    L 
Sbjct: 53  QQSFSHHKQKKLSRCKTAPAMFILPHLKPINDQPQLPKPQTNSII-------RQGIWLLL 105

Query: 76  VYVGVGALAFYLVRNQIKGLKTNRFLDALYFTIVTMTTVGYGDLVPNSNLTKLLACAFVF 135
           +Y+ VG + +        G++T+  +DALYF IVTM T+GYGD+ P + +TKL AC FV 
Sbjct: 106 IYLSVGVIIYSFNTKNFSGIETHPVVDALYFCIVTMCTIGYGDIAPLTPMTKLFACVFVL 165

Query: 136 SGMAVVGLILSKAADYLVEKQEALLVKAMHM--RENFGPSEILKEIETNK--TRYKXXXX 191
            G   + ++LS   +++++ QE  ++  + M  RE F   + + ++   +   R K    
Sbjct: 166 VGFGFIDILLSGLVNFVLDLQENTILTGLQMGAREGFTARDYIVDVAKGRMRIRLKVGLA 225

Query: 192 XXXXXXXXXXXXXXXXSVEKLDFVDAFYCVCSTITTLGYGDKSFSTQAGRVFAVIWILIG 251
                            VE LD+VD+ Y    ++TT+GYGD++F T  GR+FA IW+L  
Sbjct: 226 LGVVVLCIGVGSLVLCFVEGLDWVDSIYLAVMSVTTVGYGDRAFKTLPGRLFAAIWLLFS 285

Query: 252 TITVAQFFLYMAELNTESRQKELAKWVLERKITHXXXXXXXXXXXGTVGADEFVIYKLKE 311
           T+ VA+ FLY+AE   + R + LAK VL R+IT            G +   E+VI+KLKE
Sbjct: 286 TLMVARAFLYLAEARIDRRHRRLAKKVLHREITIEDWLAADINNTGFISKSEYVIFKLKE 345

Query: 312 MGKISQEDISLFLKEFEELDVDQSGTLSVSDITLAQS 348
           MGKI  +D+     +F +LD    G +++  +   +S
Sbjct: 346 MGKIQDKDVMQICDQFRKLDPSNCGKITLPHLLEGRS 382


>Medtr4g133820.1 | hypothetical protein | LC |
           chr4:55973846-55974579 | 20130731
          Length = 109

 Score = 54.3 bits (129), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 25/39 (64%), Positives = 31/39 (79%)

Query: 118 DLVPNSNLTKLLACAFVFSGMAVVGLILSKAADYLVEKQ 156
           DLVP S + K LAC +VF+ MA+ GLILS AADY++EKQ
Sbjct: 71  DLVPISTIEKFLACIYVFTDMALGGLILSNAADYIIEKQ 109