Miyakogusa Predicted Gene

Lj2g3v2379070.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v2379070.1 tr|J3SFI5|J3SFI5_CROAD tRNA pseudouridine
synthase OS=Crotalus adamanteus PE=2 SV=1,31.35,2e-18,Pseudouridine
synthase,Pseudouridine synthase, catalytic domain; no
description,Pseudouridine syntha,CUFF.38916.1
         (498 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g074620.1 | tRNA pseudouridine synthase | HC | chr5:317201...   669   0.0  
Medtr5g074640.1 | tRNA pseudouridine synthase | HC | chr5:317260...   539   e-153
Medtr4g131270.1 | tRNA pseudouridine synthase A, related protein...    78   3e-14
Medtr4g131220.1 | tRNA pseudouridine synthase A, related protein...    78   3e-14
Medtr4g131200.1 | tRNA pseudouridine synthase | HC | chr4:547442...    61   2e-09
Medtr4g131205.1 | tRNA pseudouridine synthase | HC | chr4:547446...    61   2e-09

>Medtr5g074620.1 | tRNA pseudouridine synthase | HC |
           chr5:31720119-31716063 | 20130731
          Length = 483

 Score =  669 bits (1726), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 322/447 (72%), Positives = 368/447 (82%), Gaps = 12/447 (2%)

Query: 52  DKWEPFLKKTVVMRVGYVGTDYRGLQIQRDEHRLSTIEKELETAIYKAGGIRDSNFGDLD 111
           +KW P+LKK VVMRVGY+GTD+RGLQIQR++H+LSTIEKELETAI+K GGIRDSN+GDLD
Sbjct: 49  EKWVPYLKKKVVMRVGYIGTDFRGLQIQRNDHKLSTIEKELETAIFKVGGIRDSNYGDLD 108

Query: 112 KVKWGRSSRTDKGVHSLATMISFKMEIPEDAWKEDRDGVVLANRINIYLPNTIRVFGILP 171
           K+ WGRSSRTDKGVHSL+TMISFKMEIPE+AWK D  G+ +AN IN YLPN+IRVF +LP
Sbjct: 109 KINWGRSSRTDKGVHSLSTMISFKMEIPENAWKGDDYGIEMANHINSYLPNSIRVFSVLP 168

Query: 172 SKKRFDPRRECNLRKYSYLLPADIIGIQSHFSEDEIDFHISEFNSLLSAFEGEHPFHNYT 231
           S KRFDPR+EC++RKYSYLLPADIIG+QSHFSEDE DFHISEFNS+L AFEG+HPFHNYT
Sbjct: 169 STKRFDPRKECSMRKYSYLLPADIIGVQSHFSEDETDFHISEFNSILGAFEGDHPFHNYT 228

Query: 232 VRSKYRKKHHARKSPGNGIMSNKTGXXXXXXXXXXXXXXXDVDDFVSTTLGDKDQXXXXX 291
           VRS YRKKHHARKSPGNG MSN TG               D DD +S   G+ +Q     
Sbjct: 229 VRSVYRKKHHARKSPGNGGMSNMTGSSSLVSACDSENEESDTDDSMS---GNGEQ----- 280

Query: 292 XXXXXNDFSHQNSSLVVRARWLYEPDEADRLNVSHFRKISQCSCGKLQRLLGKDYVEIKI 351
                 + S  NS   ++ARWL+EPD ADRLN SHFRKI +CSCGKL+ LLG +Y+EI +
Sbjct: 281 -SHKSQECSESNS---LKARWLHEPDAADRLNASHFRKILRCSCGKLETLLGYNYIEIDV 336

Query: 352 WGDSFMLHQIRKMVGTAVAVKRNLIPRDILSLSLIKFTRIVLPLAPPEVLILRGNTFSMR 411
           WGDSFMLHQIRKMVGTAVAVKRN IP+DI  LSLIKF+RIVLP+APPEVLILRGN F M 
Sbjct: 337 WGDSFMLHQIRKMVGTAVAVKRNSIPKDIFLLSLIKFSRIVLPIAPPEVLILRGNAFRMW 396

Query: 412 SSAGKFIRPEMVSLVESEPILNEVEEFYTSVMLPELSKFLDSSKSPWVEWVERLDKYSSF 471
           SS G + RPEMVS+VESE IL  V+EFYTSVMLPELSKFLDSSKSPW +WVE+LDKYSS 
Sbjct: 397 SSGGSYTRPEMVSMVESEQILKSVDEFYTSVMLPELSKFLDSSKSPWADWVEKLDKYSSI 456

Query: 472 ADAELDEVRMAWRTWKENFRAKSASDA 498
            + +L+EVR AWRTWKENFRAK AS+A
Sbjct: 457 PNDQLEEVREAWRTWKENFRAKPASEA 483


>Medtr5g074640.1 | tRNA pseudouridine synthase | HC |
           chr5:31726081-31722203 | 20130731
          Length = 501

 Score =  539 bits (1389), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 259/445 (58%), Positives = 324/445 (72%), Gaps = 10/445 (2%)

Query: 54  WEPFLKKTVVMRVGYVGTDYRGLQIQRDEHRLSTIEKELETAIYKAGGIRDSNFGDLDKV 113
           W+PF KK VVMR+ Y GT+YRGLQ+QRD++ LST+EKELE+AI+KAGGIR+SN GDL+K+
Sbjct: 51  WQPFRKKKVVMRIAYTGTNYRGLQMQRDQNSLSTVEKELESAIFKAGGIRESNLGDLEKI 110

Query: 114 KWGRSSRTDKGVHSLATMISFKMEIPEDAWKEDRDGVVLANRINIYLPNTIRVFGILPSK 173
            W RSSRTDKGVHSLATM++FKMEIPE++W ED  G  LAN +N YLP  I+V  ILP++
Sbjct: 111 GWARSSRTDKGVHSLATMVAFKMEIPENSWNEDPFGFALANYVNSYLPCDIKVISILPAQ 170

Query: 174 KRFDPRRECNLRKYSYLLPADIIGIQSHFSEDEIDFHISEFNSLLSAFEGEHPFHNYTVR 233
           + FDPR+EC LRKYSYLLPADIIGIQSH S DEID+H+SEFN +L  FEG HPFHNYT R
Sbjct: 171 RSFDPRKECVLRKYSYLLPADIIGIQSHSSNDEIDYHMSEFNDILKEFEGGHPFHNYTSR 230

Query: 234 SKYRKKHHARKSPGNGIMSNKTGXXXXXXXXXXXXXXXDVDDFVSTTL--------GDKD 285
           S+YR+  H  + P +                        VD+ ++  +        G  D
Sbjct: 231 SRYRR--HIPRRPSHSKCGEILSAYNSEQEDSDEEENFKVDEALTGNIECQNQKSSGTSD 288

Query: 286 QXXXXXXXXXXNDFSHQNSSLVVRARWLYEPDEADRLNVSHFRKISQCSCGKLQRLLGKD 345
                       +   Q+SS VVRA+WLYEPDEADRLN SHFR++ +CS GKL++ +G  
Sbjct: 289 SCEPVIETGNKGNLHDQSSSSVVRAKWLYEPDEADRLNASHFRRVFRCSSGKLEKSMGHS 348

Query: 346 YVEIKIWGDSFMLHQIRKMVGTAVAVKRNLIPRDILSLSLIKFTRIVLPLAPPEVLILRG 405
           Y+EI I G+SFMLHQIRKMV TAVAVKR L+PRDI++LSL KF+RI+LPLAP EVL+LRG
Sbjct: 349 YIEIYIEGESFMLHQIRKMVATAVAVKRKLLPRDIITLSLSKFSRIILPLAPSEVLLLRG 408

Query: 406 NTFSMRSSAGKFIRPEMVSLVESEPILNEVEEFYTSVMLPELSKFLDSSKSPWVEWVERL 465
           N+FSMR+  G   RPEM ++VESE I   V++FY SVMLP++SKFLD S+ PWVEW+E+ 
Sbjct: 409 NSFSMRTKPGNVTRPEMQTIVESEEINKVVDDFYRSVMLPQVSKFLDPSRVPWVEWIEKF 468

Query: 466 DKYSSFADAELDEVRMAWRTWKENF 490
           D ++S  + +LDEVR A   WKENF
Sbjct: 469 DAHTSIPNDQLDEVRKARNLWKENF 493


>Medtr4g131270.1 | tRNA pseudouridine synthase A, related protein |
           HC | chr4:54768312-54766231 | 20130731
          Length = 530

 Score = 77.8 bits (190), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 48/165 (29%), Positives = 80/165 (48%), Gaps = 12/165 (7%)

Query: 57  FLKKTVVMRVGYVGTDYRGLQIQRDEHRLSTIEKELETAIYKAGGIRDSNFGDLDKVKWG 116
           + ++ V +   Y G  Y+G+Q         TIE ELE A+Y +G + + + G   +  W 
Sbjct: 51  YKRRKVAIFFAYCGVGYQGMQ---KNPGAKTIEGELEEALYVSGAVPEQDRGLSKRYDWA 107

Query: 117 RSSRTDKGVHSLATMISFKMEIPEDAWKEDRDGVVLANRINIYLPNTIRVFGILPSKKRF 176
           RS+RTDKGV ++  ++S +  I       D  G V  +R+N  LP+ IR+FG       F
Sbjct: 108 RSARTDKGVSAVGQVVSGRFYI-------DPPGFV--DRLNSNLPSQIRIFGFKRVTASF 158

Query: 177 DPRRECNLRKYSYLLPADIIGIQSHFSEDEIDFHISEFNSLLSAF 221
             ++ C+ R+Y YL+P   +    H   + +   +   N L+   
Sbjct: 159 SAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCL 203



 Score = 61.6 bits (148), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 29/61 (47%), Positives = 39/61 (63%)

Query: 343 GKDYVEIKIWGDSFMLHQIRKMVGTAVAVKRNLIPRDILSLSLIKFTRIVLPLAPPEVLI 402
           G ++V+ +I G SFMLHQIRKM+G AVA+ RN  P  ++  +L K   I +P AP   L 
Sbjct: 375 GMEFVKCEIVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLY 434

Query: 403 L 403
           L
Sbjct: 435 L 435


>Medtr4g131220.1 | tRNA pseudouridine synthase A, related protein |
           HC | chr4:54751590-54749272 | 20130731
          Length = 530

 Score = 77.8 bits (190), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 48/165 (29%), Positives = 80/165 (48%), Gaps = 12/165 (7%)

Query: 57  FLKKTVVMRVGYVGTDYRGLQIQRDEHRLSTIEKELETAIYKAGGIRDSNFGDLDKVKWG 116
           + ++ V +   Y G  Y+G+Q         TIE ELE A+Y +G + + + G   +  W 
Sbjct: 51  YKRRKVAIFFAYCGVGYQGMQKNPG---AKTIEGELEEALYVSGAVPEQDRGLSKRYDWA 107

Query: 117 RSSRTDKGVHSLATMISFKMEIPEDAWKEDRDGVVLANRINIYLPNTIRVFGILPSKKRF 176
           RS+RTDKGV ++  ++S +  I       D  G V  +R+N  LP+ IR+FG       F
Sbjct: 108 RSARTDKGVSAVGQVVSGRFYI-------DPPGFV--DRLNSNLPSQIRIFGFKRVTASF 158

Query: 177 DPRRECNLRKYSYLLPADIIGIQSHFSEDEIDFHISEFNSLLSAF 221
             ++ C+ R+Y YL+P   +    H   + +   +   N L+   
Sbjct: 159 SAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCL 203



 Score = 61.6 bits (148), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 29/61 (47%), Positives = 39/61 (63%)

Query: 343 GKDYVEIKIWGDSFMLHQIRKMVGTAVAVKRNLIPRDILSLSLIKFTRIVLPLAPPEVLI 402
           G ++V+ +I G SFMLHQIRKM+G AVA+ RN  P  ++  +L K   I +P AP   L 
Sbjct: 375 GMEFVKCEIVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLY 434

Query: 403 L 403
           L
Sbjct: 435 L 435


>Medtr4g131200.1 | tRNA pseudouridine synthase | HC |
           chr4:54744263-54742890 | 20130731
          Length = 361

 Score = 61.2 bits (147), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 29/61 (47%), Positives = 39/61 (63%)

Query: 343 GKDYVEIKIWGDSFMLHQIRKMVGTAVAVKRNLIPRDILSLSLIKFTRIVLPLAPPEVLI 402
           G ++V+ +I G SFMLHQIRKM+G AVA+ RN  P  ++  +L K   I +P AP   L 
Sbjct: 206 GMEFVKCEIVGQSFMLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLY 265

Query: 403 L 403
           L
Sbjct: 266 L 266


>Medtr4g131205.1 | tRNA pseudouridine synthase | HC |
           chr4:54744679-54744275 | 20130731
          Length = 134

 Score = 61.2 bits (147), Expect = 2e-09,   Method: Composition-based stats.
 Identities = 37/112 (33%), Positives = 60/112 (53%), Gaps = 12/112 (10%)

Query: 57  FLKKTVVMRVGYVGTDYRGLQIQRDEHRLSTIEKELETAIYKAGGIRDSNFGDLDKVKWG 116
           + ++ V +   Y G  Y+G+Q         TIE ELE A+Y +G + + + G   +  W 
Sbjct: 21  YKRRKVAIFFAYCGVGYQGMQ---KNPGAKTIEGELEEALYVSGAVPEQDRGLSKRYDWA 77

Query: 117 RSSRTDKGVHSLATMISFKMEIPEDAWKEDRDGVVLANRINIYLPNTIRVFG 168
           RS+RTDKGV ++  ++S +  I       D  G V  +R+N  LP+ I++FG
Sbjct: 78  RSARTDKGVSAVGQVVSGRFYI-------DPPGFV--DRLNSNLPSQIQIFG 120