Miyakogusa Predicted Gene

Lj2g3v1728760.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v1728760.1 CUFF.37726.1
         (669 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g021230.1 | radical SAM methylthiotransferase, MiaB/RimO f...  1004   0.0  
Medtr4g033210.1 | dimethylallyl, adenosine tRNA methylthiotransf...   122   1e-27

>Medtr5g021230.1 | radical SAM methylthiotransferase, MiaB/RimO
           family protein | HC | chr5:8174329-8167505 | 20130731
          Length = 607

 Score = 1004 bits (2597), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 490/615 (79%), Positives = 528/615 (85%), Gaps = 23/615 (3%)

Query: 61  NQPHTA--LKLHPPSCFTTTLRIFYHQHKFKHSTSSLPRTYGYRASTSP---PRPLNLIT 115
           NQPHTA  LKLH PSC    LR F H+H            Y +  S  P    RPL+LIT
Sbjct: 10  NQPHTAQRLKLHSPSC----LRFFKHKH------------YAFLPSPKPFSSSRPLHLIT 53

Query: 116 ASTTFPR-STKFEGPTLRRCFIXXXXXXXXXXXXXDLVLDTEVPSTGRVYHETYGCQMNV 174
           AS TF   STK + P+LR  FI              LV DTE PSTGR+YHETYGCQMNV
Sbjct: 54  ASRTFSSLSTKPKNPSLRN-FIAQASLTASQFQQPHLVADTEAPSTGRIYHETYGCQMNV 112

Query: 175 NDMEIVLSIMKNAGYNEVVSAPESAEIIFINTCAIRENAELKVWQRLNYFWFLKRNWKAN 234
           NDMEIVLSIMKNAGYNE+V+APE+AEIIFINTCAIR+NAE+KVWQRLNYFWFLKRNWKAN
Sbjct: 113 NDMEIVLSIMKNAGYNEIVTAPENAEIIFINTCAIRDNAEMKVWQRLNYFWFLKRNWKAN 172

Query: 235 VASGRAQSLHPPKVVVLGCMAERLKEKILDADKMVDVVCGPDAYRDLPRLLEEVDDGQKG 294
           VA+GR+QSL PPKVVVLGCMAERLKEKILD+DKMVDVVCGPDAYRDLPRLLEEV+ GQKG
Sbjct: 173 VATGRSQSLRPPKVVVLGCMAERLKEKILDSDKMVDVVCGPDAYRDLPRLLEEVEYGQKG 232

Query: 295 INTLLSLEETYADINPVRISKNSVSAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVR 354
           INTLLSLEETYADINPVRIS+NS+SAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVR
Sbjct: 233 INTLLSLEETYADINPVRISQNSISAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVR 292

Query: 355 EVAELWKEGVKEVTLLGQNVNSYNDTSGFEKEVESGSNWKLSDGFSSISKVKKMGXXXXX 414
           EVAELWKEGVKEVTLLGQNVNSYND S  EKE E+GSNWKLS+GFSSISKV+  G     
Sbjct: 293 EVAELWKEGVKEVTLLGQNVNSYNDASVVEKEAEAGSNWKLSEGFSSISKVRTTGLRFSD 352

Query: 415 XXXXXXXEFPEMRFRFTSPHPKDFPDELLYLMRERHNICKVIHLPAQTGSSTVLERMRRG 474
                  EFPEMRFRFTSPHPKD+PDELLY+MRERHNICK+IHLPAQ+GSS +LERMRRG
Sbjct: 353 LLDRLSLEFPEMRFRFTSPHPKDYPDELLYIMRERHNICKLIHLPAQSGSSKMLERMRRG 412

Query: 475 YTREAYLDLVHKIRSVIPDVGLSSDFICGFCGETEEDHSDTLTLVKAVGYDMAYMFAYSM 534
           YTREAYL LV KIRS+IPDV LSSDFICGFCGETEEDHSDTLTLVK VGYDMAYMFAYSM
Sbjct: 413 YTREAYLSLVQKIRSIIPDVALSSDFICGFCGETEEDHSDTLTLVKEVGYDMAYMFAYSM 472

Query: 535 REKTHAHRNYADDVPEEVKQRRLTELIETFRESTGKCYDSQVGTTQLVLVEGPNKRAPDT 594
           REKTHAHRNY DDVPEE KQRRLTELIETFRESTGKCYD QVGTTQLVLVEGPNKRAPDT
Sbjct: 473 REKTHAHRNYVDDVPEETKQRRLTELIETFRESTGKCYDEQVGTTQLVLVEGPNKRAPDT 532

Query: 595 ELMGKSDKGHRVLFANVPIPDRDDVNTMRNPVVGDFVEVRIMRSTRASLFGESLAITKLS 654
           EL+GKSDKGHRV+F N+PI DR+DVNT RNP VGD+VEV+I RSTRASLFGE+LAITKLS
Sbjct: 533 ELIGKSDKGHRVIFVNLPISDREDVNTKRNPAVGDYVEVQITRSTRASLFGEALAITKLS 592

Query: 655 SFYNDLDKEAVACNI 669
           SFYN+L++EAVAC++
Sbjct: 593 SFYNNLEREAVACSM 607


>Medtr4g033210.1 | dimethylallyl, adenosine tRNA
           methylthiotransferase | HC | chr4:11569877-11575926 |
           20130731
          Length = 618

 Score =  122 bits (306), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 118/519 (22%), Positives = 212/519 (40%), Gaps = 88/519 (16%)

Query: 157 VPSTGRVYHETYGCQMNVNDMEIVLSIMKNAGYNEVVSAPESAEIIFINTCAIRENAELK 216
           +P T  ++ +T+GC  N +D E +   +   GY+ +   P+ A++  INTC ++  ++  
Sbjct: 48  IPGTQTIFIKTFGCSHNQSDSEYMAGQLSAFGYS-LSDNPDEADLWLINTCTVKSPSQSA 106

Query: 217 VWQRLNYFWFLKRNWKANVASGRAQSLHPPKVVVLGCMAERLKE-KILDADKMVDVVCGP 275
           +                +    + +S + P +VV GC+ +  ++ K L+   +V V    
Sbjct: 107 M----------------DTIITKGKSSNKP-LVVAGCVPQGSRDVKELEGISIVGV---- 145

Query: 276 DAYRDLPRLLEEVDDGQKGINTLLSLEETYADINPVRISKNSVSAFVSVMRGCNNMCSFC 335
              + + R++E V++  KG    L   +T   ++  ++ KN     + +  GC   C++C
Sbjct: 146 ---QQIDRVVEVVEETLKGHEVRLLTRKTLPALDLPKVRKNKFVEILPINVGCLGACTYC 202

Query: 336 IVPFTRGRERSRPVESIVREVAELWKEGVKEVTLLGQNVNSYNDTSGFEKEVESGSNWKL 395
                RG   S  ++S+   V  +  +GVKE+ L  ++  +Y                  
Sbjct: 203 KTKHARGHLGSYTIDSLAGRVKSVVSDGVKEIWLSSEDTGAYG----------------- 245

Query: 396 SDGFSSISKVKKMGXXXXXXXXXXXXEFPE-----MRFRFTSP-----HPKDFPDELLYL 445
                     + +G            E P      +R   T+P     H K+  + L   
Sbjct: 246 ----------RDIGVNLPTLLNALVAELPADGSTMLRIGMTNPPFILEHLKEIAEVL--- 292

Query: 446 MRERHN-ICKVIHLPAQTGSSTVLERMRRGYTREAYLDLVHKIRSVIPDVGLSSDFICGF 504
              RH  +   +H+P Q+GS  +L  M R YT   +  +V  +  ++P + +++D ICGF
Sbjct: 293 ---RHPCVYTFLHVPVQSGSDAILSAMNREYTVGEFRTVVDTLTELVPGMQIATDIICGF 349

Query: 505 CGETEEDHSDTLTLVKAVGYDMAYMFAYSMREKTHAHRNYADDVPEEVKQRRLTELIETF 564
            GET+ED   T+ L+K   +   ++  +  R  T A R     VP  V +RR  EL   F
Sbjct: 350 PGETDEDFEQTVNLIKEYKFSQVHISQFYPRPGTPAAR--MKKVPSNVVKRRSRELTNVF 407

Query: 565 RESTGKCYDSQVGTTQLVLVEGPNKRAPDTELMGKSDKGHRVLFANVPIPDRDDVNTMRN 624
              T   Y    G  + + +        D    G    GH   +  V +   DD      
Sbjct: 408 ESFTP--YSGMEGKVERIWIT-------DIATDGVHLVGHTKGYIQVLVIAPDD------ 452

Query: 625 PVVGDFVEVRIMRSTRASLFGESLAITKLSSFYNDLDKE 663
            ++G     +I    R S+FGE +     +S    L+K+
Sbjct: 453 -MLGSSALAKITSVGRWSVFGEVIETINPASDNKALNKQ 490