Miyakogusa Predicted Gene

Lj2g3v1728750.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v1728750.1 Non Characterized Hit- tr|I1J8P6|I1J8P6_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.1927
PE=,89.73,0,SUBFAMILY NOT NAMED,NULL; D-LACTATE DEHYDROGENASE,NULL;
FAD_PCMH,FAD-binding, type 2; FAD-binding do,CUFF.37725.1
         (558 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g021240.1 | D-2-hydroxyglutarate dehydrogenase | HC | chr5...   940   0.0  
Medtr5g021240.2 | D-2-hydroxyglutarate dehydrogenase | HC | chr5...   769   0.0  
Medtr7g107050.1 | D-lactate dehydrogenase (cytochrome) | HC | ch...   150   4e-36
Medtr7g107050.2 | D-lactate dehydrogenase (cytochrome) | HC | ch...   111   2e-24

>Medtr5g021240.1 | D-2-hydroxyglutarate dehydrogenase | HC |
           chr5:8176429-8185154 | 20130731
          Length = 561

 Score =  940 bits (2430), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 462/561 (82%), Positives = 504/561 (89%), Gaps = 3/561 (0%)

Query: 1   MMNRNTRATLRLLTPFFNPRRNPS-LLPSTPHFDENWWHSQTNIAHGEHRVSSLFNPLRF 59
           M+NRN+R TLR LT F + + NPS    S   FDEN    + N+   E++  + FNPL  
Sbjct: 1   MINRNSRNTLRFLTRFHHHQHNPSSQFTSKTVFDENCKQLKRNVLPTENKAFNFFNPLTN 60

Query: 60  SVKNQQMGVGISTGIQHKCY-ASMPGLVQRNPRFSELNDDDVRYFQEILGQKNVVQDEEK 118
             KN+Q+G GIS GI  KCY  SM G VQR+ RFSELNDDDVRYF+EILG+KNVVQDE+K
Sbjct: 61  FFKNKQLGHGISCGILQKCYYGSMGGAVQRSSRFSELNDDDVRYFEEILGKKNVVQDEDK 120

Query: 119 LSTVNTDWMHKYEGSSKLLLQPWNTDQVSQILKYCNSRSLAVVPQGGNTGLVGGSVPVFD 178
           LS  N DWMHKY+GSSKL+LQP NTDQVSQILKYCNSR LAVVPQGGNTGLVGGSVPVFD
Sbjct: 121 LSVANIDWMHKYKGSSKLILQPCNTDQVSQILKYCNSRCLAVVPQGGNTGLVGGSVPVFD 180

Query: 179 EVIVSLSSMNKVISFDKVSGILVCEAGCILENIISFLDNEGFIMPLDLGAKGSCQIGGNV 238
           EVIVSLSSMN +ISFDKVSGILVCEAGCILENI+SFLDNEGFIMPLDLGAKGSCQIGGNV
Sbjct: 181 EVIVSLSSMNNIISFDKVSGILVCEAGCILENIMSFLDNEGFIMPLDLGAKGSCQIGGNV 240

Query: 239 STNAGGLRLVRYGSLHGSVLGVEAVLADGTVLDMLKTLRKDNTGYDLKHLFIGSEGSLGI 298
           STNAGGLRLVRYGSLHG+VLGVEAVLA+GTVLDMLKTLRKDNTGYDLKHLFIGSEGSLGI
Sbjct: 241 STNAGGLRLVRYGSLHGNVLGVEAVLANGTVLDMLKTLRKDNTGYDLKHLFIGSEGSLGI 300

Query: 299 VTKVAILTPPKLSSVNVALLACKDYNCCQKLLQEAKRKLGEILSAFEFLDSQSMNLVINH 358
           VTKV+ILTPPKLSSVNVALLACKDY+CCQKLLQEAKRKLGEILSAFEFLD QSM+LV NH
Sbjct: 301 VTKVSILTPPKLSSVNVALLACKDYSCCQKLLQEAKRKLGEILSAFEFLDGQSMDLVTNH 360

Query: 359 LEGARNPLPTL-HDFYVLIETTGSDESSDKQKLEAFLLHSMENELISDGVLAQDINQASS 417
           LEGARNP  T  H+FYVLIETTGSDESSDKQKLEAFLL SMENELI+DGVLAQDINQAS+
Sbjct: 361 LEGARNPFSTSHHNFYVLIETTGSDESSDKQKLEAFLLGSMENELIADGVLAQDINQAST 420

Query: 418 FWRLREGITEALMRVGAVYKYDLSIPLENLYNLVEEMRSRLGNAANVVGYGHLGDGNLHL 477
           FWR+REGI+EALM+ GAVYKYD+SIP+ENLYNLVEEMRSRLG+AANV+GYGHLGDGNLHL
Sbjct: 421 FWRIREGISEALMKAGAVYKYDVSIPVENLYNLVEEMRSRLGDAANVIGYGHLGDGNLHL 480

Query: 478 NISVPQYDDKILSQIEPFVYEWTSKHNGSISAEHGLGLMKANEILYSKSLETVQLMASIK 537
           N+SV QYD+KILSQIEPFVYEWTSK  GSISAEHG+GLMKANEI YS+S ETVQ+MASIK
Sbjct: 481 NVSVSQYDEKILSQIEPFVYEWTSKKRGSISAEHGVGLMKANEIFYSQSHETVQMMASIK 540

Query: 538 NLLDPNHILNPYKVLPHSLIA 558
           NL+DPNHILNPYKVLPHS+ +
Sbjct: 541 NLMDPNHILNPYKVLPHSITS 561


>Medtr5g021240.2 | D-2-hydroxyglutarate dehydrogenase | HC |
           chr5:8176429-8185066 | 20130731
          Length = 459

 Score =  769 bits (1986), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 373/416 (89%), Positives = 399/416 (95%), Gaps = 1/416 (0%)

Query: 144 DQVSQILKYCNSRSLAVVPQGGNTGLVGGSVPVFDEVIVSLSSMNKVISFDKVSGILVCE 203
           + VSQILKYCNSR LAVVPQGGNTGLVGGSVPVFDEVIVSLSSMN +ISFDKVSGILVCE
Sbjct: 44  NHVSQILKYCNSRCLAVVPQGGNTGLVGGSVPVFDEVIVSLSSMNNIISFDKVSGILVCE 103

Query: 204 AGCILENIISFLDNEGFIMPLDLGAKGSCQIGGNVSTNAGGLRLVRYGSLHGSVLGVEAV 263
           AGCILENI+SFLDNEGFIMPLDLGAKGSCQIGGNVSTNAGGLRLVRYGSLHG+VLGVEAV
Sbjct: 104 AGCILENIMSFLDNEGFIMPLDLGAKGSCQIGGNVSTNAGGLRLVRYGSLHGNVLGVEAV 163

Query: 264 LADGTVLDMLKTLRKDNTGYDLKHLFIGSEGSLGIVTKVAILTPPKLSSVNVALLACKDY 323
           LA+GTVLDMLKTLRKDNTGYDLKHLFIGSEGSLGIVTKV+ILTPPKLSSVNVALLACKDY
Sbjct: 164 LANGTVLDMLKTLRKDNTGYDLKHLFIGSEGSLGIVTKVSILTPPKLSSVNVALLACKDY 223

Query: 324 NCCQKLLQEAKRKLGEILSAFEFLDSQSMNLVINHLEGARNPLPTL-HDFYVLIETTGSD 382
           +CCQKLLQEAKRKLGEILSAFEFLD QSM+LV NHLEGARNP  T  H+FYVLIETTGSD
Sbjct: 224 SCCQKLLQEAKRKLGEILSAFEFLDGQSMDLVTNHLEGARNPFSTSHHNFYVLIETTGSD 283

Query: 383 ESSDKQKLEAFLLHSMENELISDGVLAQDINQASSFWRLREGITEALMRVGAVYKYDLSI 442
           ESSDKQKLEAFLL SMENELI+DGVLAQDINQAS+FWR+REGI+EALM+ GAVYKYD+SI
Sbjct: 284 ESSDKQKLEAFLLGSMENELIADGVLAQDINQASTFWRIREGISEALMKAGAVYKYDVSI 343

Query: 443 PLENLYNLVEEMRSRLGNAANVVGYGHLGDGNLHLNISVPQYDDKILSQIEPFVYEWTSK 502
           P+ENLYNLVEEMRSRLG+AANV+GYGHLGDGNLHLN+SV QYD+KILSQIEPFVYEWTSK
Sbjct: 344 PVENLYNLVEEMRSRLGDAANVIGYGHLGDGNLHLNVSVSQYDEKILSQIEPFVYEWTSK 403

Query: 503 HNGSISAEHGLGLMKANEILYSKSLETVQLMASIKNLLDPNHILNPYKVLPHSLIA 558
             GSISAEHG+GLMKANEI YS+S ETVQ+MASIKNL+DPNHILNPYKVLPHS+ +
Sbjct: 404 KRGSISAEHGVGLMKANEIFYSQSHETVQMMASIKNLMDPNHILNPYKVLPHSITS 459


>Medtr7g107050.1 | D-lactate dehydrogenase (cytochrome) | HC |
           chr7:43648974-43639638 | 20130731
          Length = 578

 Score =  150 bits (378), Expect = 4e-36,   Method: Compositional matrix adjust.
 Identities = 123/453 (27%), Positives = 213/453 (47%), Gaps = 31/453 (6%)

Query: 115 DEEKLSTVNTDWMHKYEGSSKLLLQPWNTDQVSQILKYCNSRSLAVVPQGGNTGLVGGSV 174
           DE  +     +  HK      +++ P + ++VS+I+K CN+  + +VP GG T + G ++
Sbjct: 138 DERYIHGKPQNSFHKAVNIPDVIVYPRSEEEVSKIVKSCNNHKIPIVPYGGATSIEGHTL 197

Query: 175 PVFDEVIVSLSSMNKVISFDKVSGILVCEAGCILENIISFLDNEGFIMPLDLGAKGSCQI 234
                V + +SSM +V +       +V + G     +  +L+  G   PLD G   S  I
Sbjct: 198 SPNGGVCIDMSSMKRVKALHVDDMDVVVDPGIGWMELNEYLEPYGLFFPLDPGPGAS--I 255

Query: 235 GGNVSTNAGGLRLVRYGSLHGSVLGVEAVLADGTVLDMLKTLRKDNTGYDLKHLFIGSEG 294
           GG  +T   G   VRYG++  +V+ ++ V A+G ++      RK   GYDL  L IGSEG
Sbjct: 256 GGMCATRCSGSLAVRYGTMRDNVISLKVVFANGDIVKTASRARKSAAGYDLTRLVIGSEG 315

Query: 295 SLGIVTKVAILTPPKLSSV-NVALLACKDYNCCQKLLQEAKRKL--GEILSAFEFLDSQS 351
           +LG++T+V +    +L  +   +++A  ++   +     A   +  G  +S  E LD   
Sbjct: 316 TLGVITEVTL----RLQKIPQFSVVAMCNFPSVKDAADVAITTMNSGIQVSRVELLDEVQ 371

Query: 352 MNLVINHLEGARNP-LPTLHDFYVLIETTGSDESSDKQKLEAFLLHSMENELISDGVLAQ 410
           +   IN   G   P  PTL     + E  G++  + +Q      + S  N   SD V A+
Sbjct: 372 VK-AINIANGKNLPESPTL-----MFEFIGTEAYAREQTQIVRKIVSEHNG--SDFVFAE 423

Query: 411 DINQASSFWRLREGITEALMRVGAVYK------YDLSIPLENLYNLVEEMRSRLGNAANV 464
           +       W++R+   EAL    A+         D+ +PL +L +++ + +  L  +  V
Sbjct: 424 EPEAKKELWKVRK---EALWACFAMEPNMEAMISDVCVPLSHLADIISKSKKELDASPLV 480

Query: 465 VGY-GHLGDGNLHLNI---SVPQYDDKILSQIEPFVYEWTSKHNGSISAEHGLGLMKANE 520
                H GDGN H  I      +   +   ++  F+        G+ + EHG+G  K   
Sbjct: 481 CTVIAHAGDGNFHTVILFDPAKEEQRREAERLNHFMVHAALSLEGTCTGEHGVGTGKMKY 540

Query: 521 ILYSKSLETVQLMASIKNLLDPNHILNPYKVLP 553
           +     +E ++ M  IK++LDPN+I+NP K++P
Sbjct: 541 LEEELGVEALKTMKKIKSVLDPNNIMNPGKLIP 573


>Medtr7g107050.2 | D-lactate dehydrogenase (cytochrome) | HC |
           chr7:43649030-43639622 | 20130731
          Length = 486

 Score =  111 bits (277), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 98/366 (26%), Positives = 172/366 (46%), Gaps = 29/366 (7%)

Query: 105 EILGQKNVV--QDEEKLSTVNTDWMHKYEGSSKLLLQPWNTDQVSQILKYCNSRSLAVVP 162
           +I+ + NV    DE  +     +  HK      +++ P + ++VS+I+K CN+  + +VP
Sbjct: 126 KIICEDNVSLDYDERYIHGKPQNSFHKAVNIPDVIVYPRSEEEVSKIVKSCNNHKIPIVP 185

Query: 163 QGGNTGLVGGSVPVFDEVIVSLSSMNKVISFDKVSGILVCEAGCILENIISFLDNEGFIM 222
            GG T + G ++     V + +SSM +V +       +V + G     +  +L+  G   
Sbjct: 186 YGGATSIEGHTLSPNGGVCIDMSSMKRVKALHVDDMDVVVDPGIGWMELNEYLEPYGLFF 245

Query: 223 PLDLGAKGSCQIGGNVSTNAGGLRLVRYGSLHGSVLGVEAVLADGTVLDMLKTLRKDNTG 282
           PLD G   S  IGG  +T   G   VRYG++  +V+ ++ V A+G ++      RK   G
Sbjct: 246 PLDPGPGAS--IGGMCATRCSGSLAVRYGTMRDNVISLKVVFANGDIVKTASRARKSAAG 303

Query: 283 YDLKHLFIGSEGSLGIVTKVAILTPPKLSSV-NVALLACKDYNCCQKLLQEAKRKL--GE 339
           YDL  L IGSEG+LG++T+V +    +L  +   +++A  ++   +     A   +  G 
Sbjct: 304 YDLTRLVIGSEGTLGVITEVTL----RLQKIPQFSVVAMCNFPSVKDAADVAITTMNSGI 359

Query: 340 ILSAFEFLDSQSMNLVINHLEGARNP-LPTLHDFYVLIETTGSDESSDKQKLEAFLLHSM 398
            +S  E LD   +   IN   G   P  PTL     + E  G++  + +Q      + S 
Sbjct: 360 QVSRVELLDEVQVK-AINIANGKNLPESPTL-----MFEFIGTEAYAREQTQIVRKIVSE 413

Query: 399 ENELISDGVLAQDINQASSFWRLREGITEALMRVGAVYK------YDLSIPLENLYNLVE 452
            N   SD V A++       W++R+   EAL    A+         D+ +PL +L +++ 
Sbjct: 414 HNG--SDFVFAEEPEAKKELWKVRK---EALWACFAMEPNMEAMISDVCVPLSHLADIIS 468

Query: 453 EMRSRL 458
           + +  L
Sbjct: 469 KSKKEL 474