Miyakogusa Predicted Gene

Lj2g3v1280020.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v1280020.1 Non Characterized Hit- tr|I3T7X2|I3T7X2_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,100,0,SUR2
HYDROXYLASE/DESATURASE,NULL; STEROL DESATURASE,NULL;
FA_hydroxylase,Fatty acid hydroxylase; seg,CUFF.36694.1
         (158 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g040320.1 | sphingoid base hydroxylase-like protein | HC |...   283   4e-77
Medtr1g097310.2 | sphingoid base hydroxylase-like protein | HC |...   253   5e-68
Medtr1g097310.1 | sphingoid base hydroxylase-like protein | HC |...   253   5e-68
Medtr1g097320.1 | sphingoid base hydroxylase-like protein | HC |...   165   1e-41
Medtr5g035190.1 | C-4 methylsterol oxidase | HC | chr5:15313529-...    68   4e-12
Medtr5g035190.2 | C-4 methylsterol oxidase | HC | chr5:15314728-...    67   5e-12
Medtr8g063210.1 | C-4 methylsterol oxidase | HC | chr8:26467122-...    67   8e-12
Medtr6g039970.1 | C-4 methylsterol oxidase | HC | chr6:14370848-...    66   2e-11
Medtr4g102060.1 | sterol C4-methyl oxidase 1-2 | HC | chr4:42239...    50   6e-07

>Medtr5g040320.1 | sphingoid base hydroxylase-like protein | HC |
           chr5:17732499-17735533 | 20130731
          Length = 263

 Score =  283 bits (725), Expect = 4e-77,   Method: Compositional matrix adjust.
 Identities = 131/158 (82%), Positives = 137/158 (86%)

Query: 1   MLVMDTWQYFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGLLNDTVGGALSF 60
           MLVMDTWQYF+HRYMHQNKFLYKHIHS HHRLIVPYSFGALYNHP+EGLL DT+GGALSF
Sbjct: 106 MLVMDTWQYFMHRYMHQNKFLYKHIHSLHHRLIVPYSFGALYNHPIEGLLLDTIGGALSF 165

Query: 61  LISGMXXXXXXXXXXXXTIKTVDDHCGLWLPGNLFQMFFQNNCAYHDVHHQLYGNKYNFS 120
           L+SGM            TIKTVDDHCGLWLPGNLF MFF NN AYHDVHHQLYGNKYNFS
Sbjct: 166 LLSGMSPRASIFFFSFATIKTVDDHCGLWLPGNLFHMFFNNNSAYHDVHHQLYGNKYNFS 225

Query: 121 QPFFVMWDRILGTYMPYRLEKREGGGFESRPCKDYKDE 158
           QPFFVMWD+ILGT+MPY LEKR  GGFESRPCK YKDE
Sbjct: 226 QPFFVMWDKILGTHMPYSLEKRASGGFESRPCKAYKDE 263


>Medtr1g097310.2 | sphingoid base hydroxylase-like protein | HC |
           chr1:43897435-43901038 | 20130731
          Length = 255

 Score =  253 bits (646), Expect = 5e-68,   Method: Compositional matrix adjust.
 Identities = 113/154 (73%), Positives = 128/154 (83%)

Query: 1   MLVMDTWQYFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGLLNDTVGGALSF 60
           M VMDTWQYF+HRYMHQNKFLY+HIHSQHHRL+VPY+ GALYNHP+EGLL DTVGGALSF
Sbjct: 102 MFVMDTWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPIEGLLLDTVGGALSF 161

Query: 61  LISGMXXXXXXXXXXXXTIKTVDDHCGLWLPGNLFQMFFQNNCAYHDVHHQLYGNKYNFS 120
           L+SGM             +KTVDDHCGLWLPGN+F +FFQNN AYHD+HHQL G KYN+S
Sbjct: 162 LVSGMTARTAVIFFCFAVVKTVDDHCGLWLPGNIFHLFFQNNTAYHDIHHQLQGLKYNYS 221

Query: 121 QPFFVMWDRILGTYMPYRLEKREGGGFESRPCKD 154
           QPFF +WD+ILGTYMP+ L KR  GGFE+RP KD
Sbjct: 222 QPFFPIWDKILGTYMPFNLVKRAEGGFEARPAKD 255


>Medtr1g097310.1 | sphingoid base hydroxylase-like protein | HC |
           chr1:43897127-43901038 | 20130731
          Length = 255

 Score =  253 bits (646), Expect = 5e-68,   Method: Compositional matrix adjust.
 Identities = 113/154 (73%), Positives = 128/154 (83%)

Query: 1   MLVMDTWQYFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGLLNDTVGGALSF 60
           M VMDTWQYF+HRYMHQNKFLY+HIHSQHHRL+VPY+ GALYNHP+EGLL DTVGGALSF
Sbjct: 102 MFVMDTWQYFVHRYMHQNKFLYRHIHSQHHRLVVPYAIGALYNHPIEGLLLDTVGGALSF 161

Query: 61  LISGMXXXXXXXXXXXXTIKTVDDHCGLWLPGNLFQMFFQNNCAYHDVHHQLYGNKYNFS 120
           L+SGM             +KTVDDHCGLWLPGN+F +FFQNN AYHD+HHQL G KYN+S
Sbjct: 162 LVSGMTARTAVIFFCFAVVKTVDDHCGLWLPGNIFHLFFQNNTAYHDIHHQLQGLKYNYS 221

Query: 121 QPFFVMWDRILGTYMPYRLEKREGGGFESRPCKD 154
           QPFF +WD+ILGTYMP+ L KR  GGFE+RP KD
Sbjct: 222 QPFFPIWDKILGTYMPFNLVKRAEGGFEARPAKD 255


>Medtr1g097320.1 | sphingoid base hydroxylase-like protein | HC |
           chr1:43904447-43904953 | 20130731
          Length = 143

 Score =  165 bits (418), Expect = 1e-41,   Method: Compositional matrix adjust.
 Identities = 75/105 (71%), Positives = 84/105 (80%)

Query: 1   MLVMDTWQYFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGLLNDTVGGALSF 60
           M VMDTWQYF+H YMHQNKFLY HIHSQHHRL+VPY+ GALYNHP+E LL DTVGGA+SF
Sbjct: 31  MFVMDTWQYFVHWYMHQNKFLYCHIHSQHHRLVVPYAIGALYNHPIESLLLDTVGGAISF 90

Query: 61  LISGMXXXXXXXXXXXXTIKTVDDHCGLWLPGNLFQMFFQNNCAY 105
           L+SGM             +KTVDDHCGLWLPGN+F +FFQNN AY
Sbjct: 91  LVSGMTARTAVIFFCFDVVKTVDDHCGLWLPGNIFHLFFQNNTAY 135


>Medtr5g035190.1 | C-4 methylsterol oxidase | HC |
           chr5:15313529-15317196 | 20130731
          Length = 271

 Score = 68.2 bits (165), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 54/157 (34%), Positives = 73/157 (46%), Gaps = 19/157 (12%)

Query: 9   YFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGL---LNDTVGGALSFLISGM 65
           Y+ HR +H  K+LYKHIHS HH    P+   + Y HP E L       VG A    I+G 
Sbjct: 124 YWGHRILH-TKWLYKHIHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA----ITGP 178

Query: 66  XXXXXXXXXXXXTIKTVDDHCGL---WLPGNLFQMFFQNNCAYHDVHHQ-LYGNKYNFSQ 121
                        ++TV+ HCG    W P N   ++      +HD HH+ LY    N+S 
Sbjct: 179 HLITLWLWMVVRVLETVEAHCGYHFPWSPSNFLPLY--GGSDFHDYHHRLLYTKSGNYSS 236

Query: 122 PFFVMWDRILGTYMPYRLEKREGGGFESRPCKDYKDE 158
            F  M DRI GT + YR  K      +SR  +D  ++
Sbjct: 237 TFTYM-DRIFGTDVGYRKLK----ALKSREFEDSYEQ 268


>Medtr5g035190.2 | C-4 methylsterol oxidase | HC |
           chr5:15314728-15317193 | 20130731
          Length = 217

 Score = 67.4 bits (163), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 54/153 (35%), Positives = 71/153 (46%), Gaps = 19/153 (12%)

Query: 9   YFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGL---LNDTVGGALSFLISGM 65
           Y+ HR +H  K+LYKHIHS HH    P+   + Y HP E L       VG A    I+G 
Sbjct: 70  YWGHRILH-TKWLYKHIHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA----ITGP 124

Query: 66  XXXXXXXXXXXXTIKTVDDHCGL---WLPGNLFQMFFQNNCAYHDVHHQ-LYGNKYNFSQ 121
                        ++TV+ HCG    W P N   ++      +HD HH+ LY    N+S 
Sbjct: 125 HLITLWLWMVVRVLETVEAHCGYHFPWSPSNFLPLY--GGSDFHDYHHRLLYTKSGNYSS 182

Query: 122 PFFVMWDRILGTYMPYRLEKREGGGFESRPCKD 154
            F  M DRI GT + YR  K      +SR  +D
Sbjct: 183 TFTYM-DRIFGTDVGYRKLK----ALKSREFED 210


>Medtr8g063210.1 | C-4 methylsterol oxidase | HC |
           chr8:26467122-26463827 | 20130731
          Length = 271

 Score = 67.0 bits (162), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 49/137 (35%), Positives = 65/137 (47%), Gaps = 15/137 (10%)

Query: 9   YFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGL---LNDTVGGALSFLISGM 65
           Y+ HR +H  K+LYKH+HS HH    P+   + Y HP E L       VG A    I+G 
Sbjct: 124 YWGHRILH-TKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPA----ITGP 178

Query: 66  XXXXXXXXXXXXTIKTVDDHCGL---WLPGNLFQMFFQNNCAYHDVHHQ-LYGNKYNFSQ 121
                        ++TV+ HCG    W P N   ++      +HD HH+ LY    N+S 
Sbjct: 179 HLITLWLWMVLRVLETVEAHCGYHFPWSPSNFLPLY--GGSDFHDYHHRLLYTKSGNYSS 236

Query: 122 PFFVMWDRILGTYMPYR 138
            F  M DRI GT + YR
Sbjct: 237 TFTYM-DRIFGTDIGYR 252


>Medtr6g039970.1 | C-4 methylsterol oxidase | HC |
           chr6:14370848-14368250 | 20130731
          Length = 271

 Score = 65.9 bits (159), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 50/145 (34%), Positives = 68/145 (46%), Gaps = 10/145 (6%)

Query: 9   YFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGLLNDTVGGALSFLISGMXXX 68
           Y+ HR MH  K+LYKH+H  HH    P+   + Y HP E +L       L   I+G    
Sbjct: 124 YWEHRIMH-TKWLYKHVHRVHHEYATPFGLTSEYGHPAE-ILFLGFPTMLGPAITGPHLI 181

Query: 69  XXXXXXXXXTIKTVDDHCGL---WLPGNLFQMFFQNNCAYHDVHHQ-LYGNKYNFSQPFF 124
                     ++TV+ HCG    W P N   ++      +HD HH+ LY    N+S  F 
Sbjct: 182 TLWLYTVLRVLETVEAHCGYHFPWSPSNFLPLY--GGADFHDYHHRVLYTKSGNYSSTFT 239

Query: 125 VMWDRILGTYMPYR-LEKREGGGFE 148
            M DRI GT + YR L+  +  G E
Sbjct: 240 YM-DRIFGTDIGYRKLKALKNTGVE 263


>Medtr4g102060.1 | sterol C4-methyl oxidase 1-2 | HC |
           chr4:42239567-42243413 | 20130731
          Length = 303

 Score = 50.4 bits (119), Expect = 6e-07,   Method: Compositional matrix adjust.
 Identities = 44/145 (30%), Positives = 61/145 (42%), Gaps = 9/145 (6%)

Query: 2   LVMDTWQYFLHRYMHQNKFLYKHIHSQHHRLIVPYSFGALYNHPVEGLLNDTVGGALSFL 61
           LV D   Y++HR++H NK+ Y+ IH  HH    P  F A Y H  E L    + G  SFL
Sbjct: 135 LVEDYTNYWIHRFLH-NKWGYEKIHRVHHEYQAPIGFAAPYAHWAEIL----ILGIPSFL 189

Query: 62  ISGMX---XXXXXXXXXXXTIKTVDDHCGLWLPGNLFQMF-FQNNCAYHDVHHQLYGNKY 117
              M                ++ +D H G   P N  +   F     +HD HH + G   
Sbjct: 190 GPAMVPGHMITFWLWIALRQVEAIDTHSGYDFPWNPTKYIPFYGGAEHHDYHHYVGGQSQ 249

Query: 118 NFSQPFFVMWDRILGTYMPYRLEKR 142
           +     F   D I GT   +R +K+
Sbjct: 250 SNFASVFTYCDYIYGTDKGFRYQKK 274