Miyakogusa Predicted Gene

Lj2g3v1253990.1
Show Alignment:  
BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v1253990.1 Non Characterized Hit- tr|I3S5R3|I3S5R3_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,98.9,0,COBW
DOMAIN CONTAINING (VERTEBRATES),NULL; COBW-RELATED,NULL; Cobalamin
synthesis protein cobW
C-ter,NODE_81045_length_1593_cov_18.831764.path2.1
         (364 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g044050.1 | cobalamin biosynthesis protein | HC | chr5:193...   635   0.0  
Medtr8g071110.1 | prli-interacting factor L-like protein | HC | ...   231   1e-60
Medtr1g115505.1 | cobalamin biosynthesis protein | HC | chr1:521...   176   3e-44
Medtr5g021440.1 | cobalamin biosynthesis CobW-like protein, puta...    67   3e-11

>Medtr5g044050.1 | cobalamin biosynthesis protein | HC |
           chr5:19367945-19374070 | 20130731
          Length = 366

 Score =  635 bits (1639), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 312/366 (85%), Positives = 332/366 (90%), Gaps = 2/366 (0%)

Query: 1   MEHDEDEEAPLAVQIQ--GLDESVSQQSSVGVTLITGYLGAGKSTLVNHILNSQHGKRIA 58
           MEHDEDEE PLAV+IQ    DES+SQQSSVGVTLITGYLGAGKSTLVNHILNSQHGKRIA
Sbjct: 1   MEHDEDEEPPLAVEIQQHNNDESISQQSSVGVTLITGYLGAGKSTLVNHILNSQHGKRIA 60

Query: 59  VILNEFGEEIGVERAMINEGDGGAMVEEWVELANGCICCTVKHSLVQALEQLVQRKERLD 118
           VILNEFGEEIGVERA+INEG+ GA+VEEWVELANGCICCTVKHSLVQALEQLVQRKERLD
Sbjct: 61  VILNEFGEEIGVERALINEGESGAVVEEWVELANGCICCTVKHSLVQALEQLVQRKERLD 120

Query: 119 HILLETTGLANPAPLASVLWLDEQLESDVKLDSIVTVVDAKNVRLQIDQHRRSSSYPEAY 178
           HILLETTGLANPAPLAS+LWLDEQLESDVKLDSIVTVVDAKNVR Q+ +HR SSS+PEAY
Sbjct: 121 HILLETTGLANPAPLASILWLDEQLESDVKLDSIVTVVDAKNVRFQLKEHRGSSSFPEAY 180

Query: 179 FQIAFADIIILNKVDLVSGEGSGALKELEEEIHNINSLVEIIHSVKCQVDLSKILNRHAY 238
           FQIAFADI+ILNKVDLVS EGSGAL+ELEEEIHNIN+LVEIIHSV+CQVDLSKILNRHAY
Sbjct: 181 FQIAFADIVILNKVDLVSAEGSGALEELEEEIHNINTLVEIIHSVRCQVDLSKILNRHAY 240

Query: 239 DTARAPHXXXXXXXXXXXXXXXXHDSGVRTICISEQKTIDIDKTRIWLEEILWEKKYDMD 298
           DTA A H                HDSGVRTICI E  TID+DKTRIWLEEILWEKKYDMD
Sbjct: 241 DTAHAAHLEALLEESRSLSTKKLHDSGVRTICICETGTIDLDKTRIWLEEILWEKKYDMD 300

Query: 299 VYRCKGVLSVQNSDQLHTLQAVRELYEIVPARKWEREENRMNKIVFIGHNLKEDVLIHSF 358
           VYRCKGVL+VQNSD+LHTLQAVRELYEIVPARKW++EENRMNKIVFIGHNLKEDVLI+S 
Sbjct: 301 VYRCKGVLNVQNSDELHTLQAVRELYEIVPARKWKKEENRMNKIVFIGHNLKEDVLINSL 360

Query: 359 RACVTC 364
           +A  TC
Sbjct: 361 KALATC 366


>Medtr8g071110.1 | prli-interacting factor L-like protein | HC |
           chr8:30172455-30180031 | 20130731
          Length = 407

 Score =  231 bits (588), Expect = 1e-60,   Method: Compositional matrix adjust.
 Identities = 137/357 (38%), Positives = 204/357 (57%), Gaps = 21/357 (5%)

Query: 20  ESVSQQSSVGVTLITGYLGAGKSTLVNHILNSQHGKRIAVILNEFGEEIGVERAMINEGD 79
           E  S  + +  T+ITG+LG+GK+TL+NHIL SQHGKRIAVI NEFGE + ++ +++    
Sbjct: 57  EEASVDNRIPATIITGFLGSGKTTLLNHILTSQHGKRIAVIENEFGE-VDIDGSLV-ASH 114

Query: 80  GGAMVEEWVELANGCICCTVKHSLVQALEQLVQRK-ERLDHILLETTGLANPAPLASVLW 138
                E+ + + NGC+CCTV+  LV+ L +LV++K ++ DHI++ETTGLA PAP+    +
Sbjct: 115 SSVNGEDIIMVNNGCLCCTVRGDLVKMLLELVRKKRDKFDHIVIETTGLAKPAPVIETFF 174

Query: 139 LDEQLESDVKLDSIVTVVDAKNVRLQIDQHRRSSSYPEAYFQIAFADIIILNKVDLVSGE 198
            DE +   VKLD +VT+VD K+    +++ +      EA  Q+A+AD IILNK+DLV+  
Sbjct: 175 SDELVSEYVKLDGVVTLVDCKHAMQHLNEVKPRFVVNEAVEQVAYADRIILNKIDLVT-- 232

Query: 199 GSGALKELEEEIHNINSLVEIIHSVKCQVDLSKILNRHAYDTAR------------APHX 246
               L  L ++I +IN + +I  +    VD+  +L    YD  R            A H 
Sbjct: 233 -ESELNMLTKKIKHINGMAQIKQAKFGSVDIDFVLGVGGYDIQRIEAEVEGECPSTASHP 291

Query: 247 XX-XXXXXXXXXXXXXHDSGVRTICISEQKTIDIDKTRIWLEEILWEKKYDMDVYRCKGV 305
                           HDS V ++ I  + T+D D+   WLE ++ EK  D+  YR KGV
Sbjct: 292 DDCGHEHKGHHHHDHVHDSAVSSVSIVAEGTLDRDEVEDWLERLVEEKGEDL--YRMKGV 349

Query: 306 LSVQNSDQLHTLQAVRELYEIVPARKWEREENRMNKIVFIGHNLKEDVLIHSFRACV 362
           L+V  S+Q +  Q V  + +  P + W+  E R+NK+VFIG NL E  L   F+ C+
Sbjct: 350 LAVDGSEQRYVFQGVHSMLDDSPGKTWQPNEKRINKLVFIGRNLDETALRKGFKGCL 406


>Medtr1g115505.1 | cobalamin biosynthesis protein | HC |
           chr1:52194306-52199057 | 20130731
          Length = 449

 Score =  176 bits (446), Expect = 3e-44,   Method: Compositional matrix adjust.
 Identities = 95/222 (42%), Positives = 144/222 (64%), Gaps = 7/222 (3%)

Query: 22  VSQQSSVGVTLITGYLGAGKSTLVNHILNSQHGKRIAVILNEFGEEIGVERAMINEGDGG 81
           +   + +  T+ITG+LG+GK+TL+NHIL ++HGKRIAVI NEFGE I ++ +++     G
Sbjct: 83  IPPDNRIPATIITGFLGSGKTTLLNHILTAEHGKRIAVIENEFGE-IDIDGSLVAAKTAG 141

Query: 82  AMVEEWVELANGCICCTVKHSLVQALEQLV-QRKERLDHILLETTGLANPAPLASVLWLD 140
           A  E+ + L NGC+CCTV+  LV+ + +LV  +K + DHI++ETTGLANPAP+    + +
Sbjct: 142 A--EDIMLLNNGCLCCTVRGDLVRMISELVTSKKGKFDHIVIETTGLANPAPIIQTFYAE 199

Query: 141 EQLESDVKLDSIVTVVDAKNVRLQIDQHRRSSSYPEAYFQIAFADIIILNKVDLVSGEGS 200
           + + ++VKLD +VT+VDAK+  L +D+ +      EA  QIA+AD II+NK DLV   G 
Sbjct: 200 DTIFNEVKLDGVVTLVDAKHAGLHLDEVKPKGVVNEAVEQIAYADRIIVNKTDLV---GE 256

Query: 201 GALKELEEEIHNINSLVEIIHSVKCQVDLSKILNRHAYDTAR 242
             +  L + I  INSL  +  +   +V+L  +L    +D  R
Sbjct: 257 SDITSLVQRIRKINSLANLKRTEYGKVNLDYVLGIGGFDLER 298



 Score = 79.7 bits (195), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 37/98 (37%), Positives = 59/98 (60%), Gaps = 2/98 (2%)

Query: 265 GVRTICISEQKTIDIDKTRIWLEEILWEKKYDMDVYRCKGVLSVQNSDQLHTLQAVRELY 324
           GV ++ I  +  +D++K  +WL  +L E+  D  +YR KG+LSVQ  ++    Q V +++
Sbjct: 353 GVSSVSIVCEGNLDLEKANMWLGTLLMERSDD--IYRMKGLLSVQGMNERFVFQGVHDIF 410

Query: 325 EIVPARKWEREENRMNKIVFIGHNLKEDVLIHSFRACV 362
           +  P R W+ +E R NKIVFIG NL  + L   F+ C+
Sbjct: 411 QGSPERLWQPDEPRTNKIVFIGKNLNAEELEKGFKTCL 448


>Medtr5g021440.1 | cobalamin biosynthesis CobW-like protein,
          putative | HC | chr5:8284612-8284415 | 20130731
          Length = 65

 Score = 66.6 bits (161), Expect = 3e-11,   Method: Composition-based stats.
 Identities = 36/48 (75%), Positives = 40/48 (83%), Gaps = 3/48 (6%)

Query: 1  MEHDEDEEAPLAVQIQ--GLDESVSQQSSVGVTLITGYLGAGKSTLVN 46
          MEHDE EE PLAV+IQ    DES+SQQSSVGVTLI GYLGAGKST+ +
Sbjct: 1  MEHDE-EEPPLAVEIQQHNNDESISQQSSVGVTLIIGYLGAGKSTITH 47