Miyakogusa Predicted Gene

Lj2g3v0915080.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v0915080.1 tr|Q5KR50|Q5KR50_LOTJA Sphingosine kinase
OS=Lotus japonicus GN=LjLCBK1 PE=2 SV=1,99.75,0,Diacylglycerol kinase
catalytic domain (pres,Diacylglycerol kinase, catalytic domain;
DAGK,Diacylgly,CUFF.36030.1
         (788 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr6g004070.1 | sphingoid long-chain bases kinase-like protein...  1206   0.0  
Medtr6g004070.2 | sphingoid long-chain bases kinase-like protein...  1116   0.0  
Medtr2g011820.1 | D-erythro-sphingosine kinase/diacylglycerol ki...    67   6e-11
Medtr3g008260.1 | ceramide kinase-like protein | HC | chr3:13890...    60   1e-08
Medtr3g008260.2 | ceramide kinase-like protein | HC | chr3:13890...    59   1e-08

>Medtr6g004070.1 | sphingoid long-chain bases kinase-like protein |
           HC | chr6:102237-110525 | 20130731
          Length = 777

 Score = 1206 bits (3119), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 606/754 (80%), Positives = 647/754 (85%), Gaps = 16/754 (2%)

Query: 40  PQQTLRRLGLCSQIAT-GEQTSPVVFPEKXXXXXXXXXXXXXXXXXXPDEQDAVV-KNFE 97
           PQQ+ RRLGLCSQ++T GE +SP+VFPEK                   D QDA   KNFE
Sbjct: 35  PQQSFRRLGLCSQLSTAGEHSSPIVFPEKRSKLKASKKNSTEAIRASGDHQDAAAAKNFE 94

Query: 98  HRIDIXXXXXXXXXXXDEKSDLLGYVVFSGKLLFDKRKAAVNKND--DAQQGSS-DITKQ 154
           HRIDI           DEKSDLLGYVVFSGKL  DKRK +VN N+  DAQQ +S D T Q
Sbjct: 95  HRIDIPGGG-------DEKSDLLGYVVFSGKLALDKRKISVNNNNKTDAQQQTSFDTTNQ 147

Query: 155 GAVDAKLTSKALLWGSKVLHLDDVISVSYNVGFRHFTVHSYPMNKASCGLSCFIKSRRSR 214
            AVDAKLTSKALLWGS VLHLDDVISVSY+ G +HFTVHSYPM KASCGLSCFIK RRSR
Sbjct: 148 AAVDAKLTSKALLWGSHVLHLDDVISVSYHAGLKHFTVHSYPMKKASCGLSCFIKPRRSR 207

Query: 215 KDFRFVASNVEEALQWVGGFADQQCFVNCLPHPLGSSKKQASQELLRTDMPPELIFRCKT 274
           KDFRFVAS +EEA+QWVGGFADQ CF+NCLPHPL SSKKQAS EL +TD PPEL+FRCKT
Sbjct: 208 KDFRFVASTIEEAIQWVGGFADQHCFINCLPHPLVSSKKQASSELFQTDTPPELLFRCKT 267

Query: 275 PPRMLVILNPRSGRGRSSKVFHGIVEPIFKLAGFRLEVVKTTSAGHARSLASSVDISTCP 334
           PP+MLVILNPRSGRGRSSKVFH  VEPIF+LAGFRLEVVKTTSAGHAR+LAS+VDISTCP
Sbjct: 268 PPKMLVILNPRSGRGRSSKVFHRDVEPIFRLAGFRLEVVKTTSAGHARNLASTVDISTCP 327

Query: 335 XXXXXXXXXXXXNEVLNGLLSRDNQKEXXXXXXXXXXXXXDNSLVWTVLGVRDPVSAAIA 394
                       NEV+NGLLSRDNQKE             DNSLVWTVLGVRDPVSAA+A
Sbjct: 328 DGIICVGGDGIINEVVNGLLSRDNQKEGISIPIGIIPAGSDNSLVWTVLGVRDPVSAAMA 387

Query: 395 IVKGGLTATDVFAVEWAQTNKVHFGLTVSYYGFVGDVLELSEKYQKRFGPLRYFVAGFLK 454
           IVKGGLTATDVFAVEW Q NK+HFGLTVSYYGFV DVLELSEKYQKRFGPLRYFVAGFLK
Sbjct: 388 IVKGGLTATDVFAVEWIQNNKIHFGLTVSYYGFVSDVLELSEKYQKRFGPLRYFVAGFLK 447

Query: 455 FLCLPRYSYEIEYLPASKTEREGKLSGEREVVDMSDLYTDIMGRTNKEGMPRASSLSSID 514
           FLCLPRYSYE+EYLPASK E+EG  SGEREVVDMS+LYTDIM R+NK+GMPRASSLSSID
Sbjct: 448 FLCLPRYSYEVEYLPASKPEKEG--SGEREVVDMSELYTDIMTRSNKDGMPRASSLSSID 505

Query: 515 SIMTPSRMSGGDLDTCSSTHASTEPSELVRGLDPKSKRLSSGRSNVTAEPEVIHPQLPLS 574
           SIMTPSR+SGGDLDTCSSTHASTEPSELVRGLDPKSKRLSSGRSN+TAEPEVIHPQLPLS
Sbjct: 506 SIMTPSRISGGDLDTCSSTHASTEPSELVRGLDPKSKRLSSGRSNITAEPEVIHPQLPLS 565

Query: 575 TTPNWPRTRSKSRNDKGWTGLTTTHDTSKWGNTTTNDREDISSTLSDPGPIWDAEPKWDA 634
           TTPNWPRTRSKSRND+GW GLTTTHDTSKWGN   NDREDISSTLSDPGPIWDAEPKWD 
Sbjct: 566 TTPNWPRTRSKSRNDRGWAGLTTTHDTSKWGN-AANDREDISSTLSDPGPIWDAEPKWDP 624

Query: 635 EPTNWDVENPIELPGPSDDAEVGSTKEVVPHFGDKWVVSKGQFLGILVCNHACRTVQSSQ 694
           E  NWDVENPIELPGP DD EVGSTKEVVP FG+KWVVSKGQF+GILVCNHACRTVQ SQ
Sbjct: 625 E-ANWDVENPIELPGPPDDTEVGSTKEVVPRFGEKWVVSKGQFVGILVCNHACRTVQGSQ 683

Query: 695 VVAPKAEHDDNTLDLVLVHGNGRLKLIRFFVLLQMGRHLSLPYVENIKVKSVRIKPGKHT 754
           VVAPKAEHDDNTLDL++VHGNGRL+L+RFF+LLQMG+H+SLPYVE +KVKSVRIK GKHT
Sbjct: 684 VVAPKAEHDDNTLDLIMVHGNGRLRLLRFFILLQMGQHVSLPYVEYVKVKSVRIKSGKHT 743

Query: 755 HNGCGIDGELFPLNGQVISSLLPEQCRLIGRFRI 788
           H+GCGIDGE FPLNGQVISSLLPEQCRLIGRFRI
Sbjct: 744 HSGCGIDGEFFPLNGQVISSLLPEQCRLIGRFRI 777


>Medtr6g004070.2 | sphingoid long-chain bases kinase-like protein |
           HC | chr6:102250-110482 | 20130731
          Length = 739

 Score = 1116 bits (2887), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 563/709 (79%), Positives = 603/709 (85%), Gaps = 16/709 (2%)

Query: 40  PQQTLRRLGLCSQIAT-GEQTSPVVFPEKXXXXXXXXXXXXXXXXXXPDEQDAVV-KNFE 97
           PQQ+ RRLGLCSQ++T GE +SP+VFPEK                   D QDA   KNFE
Sbjct: 35  PQQSFRRLGLCSQLSTAGEHSSPIVFPEKRSKLKASKKNSTEAIRASGDHQDAAAAKNFE 94

Query: 98  HRIDIXXXXXXXXXXXDEKSDLLGYVVFSGKLLFDKRKAAVNKND--DAQQGSS-DITKQ 154
           HRIDI           DEKSDLLGYVVFSGKL  DKRK +VN N+  DAQQ +S D T Q
Sbjct: 95  HRIDIPGGG-------DEKSDLLGYVVFSGKLALDKRKISVNNNNKTDAQQQTSFDTTNQ 147

Query: 155 GAVDAKLTSKALLWGSKVLHLDDVISVSYNVGFRHFTVHSYPMNKASCGLSCFIKSRRSR 214
            AVDAKLTSKALLWGS VLHLDDVISVSY+ G +HFTVHSYPM KASCGLSCFIK RRSR
Sbjct: 148 AAVDAKLTSKALLWGSHVLHLDDVISVSYHAGLKHFTVHSYPMKKASCGLSCFIKPRRSR 207

Query: 215 KDFRFVASNVEEALQWVGGFADQQCFVNCLPHPLGSSKKQASQELLRTDMPPELIFRCKT 274
           KDFRFVAS +EEA+QWVGGFADQ CF+NCLPHPL SSKKQAS EL +TD PPEL+FRCKT
Sbjct: 208 KDFRFVASTIEEAIQWVGGFADQHCFINCLPHPLVSSKKQASSELFQTDTPPELLFRCKT 267

Query: 275 PPRMLVILNPRSGRGRSSKVFHGIVEPIFKLAGFRLEVVKTTSAGHARSLASSVDISTCP 334
           PP+MLVILNPRSGRGRSSKVFH  VEPIF+LAGFRLEVVKTTSAGHAR+LAS+VDISTCP
Sbjct: 268 PPKMLVILNPRSGRGRSSKVFHRDVEPIFRLAGFRLEVVKTTSAGHARNLASTVDISTCP 327

Query: 335 XXXXXXXXXXXXNEVLNGLLSRDNQKEXXXXXXXXXXXXXDNSLVWTVLGVRDPVSAAIA 394
                       NEV+NGLLSRDNQKE             DNSLVWTVLGVRDPVSAA+A
Sbjct: 328 DGIICVGGDGIINEVVNGLLSRDNQKEGISIPIGIIPAGSDNSLVWTVLGVRDPVSAAMA 387

Query: 395 IVKGGLTATDVFAVEWAQTNKVHFGLTVSYYGFVGDVLELSEKYQKRFGPLRYFVAGFLK 454
           IVKGGLTATDVFAVEW Q NK+HFGLTVSYYGFV DVLELSEKYQKRFGPLRYFVAGFLK
Sbjct: 388 IVKGGLTATDVFAVEWIQNNKIHFGLTVSYYGFVSDVLELSEKYQKRFGPLRYFVAGFLK 447

Query: 455 FLCLPRYSYEIEYLPASKTEREGKLSGEREVVDMSDLYTDIMGRTNKEGMPRASSLSSID 514
           FLCLPRYSYE+EYLPASK E+EG  SGEREVVDMS+LYTDIM R+NK+GMPRASSLSSID
Sbjct: 448 FLCLPRYSYEVEYLPASKPEKEG--SGEREVVDMSELYTDIMTRSNKDGMPRASSLSSID 505

Query: 515 SIMTPSRMSGGDLDTCSSTHASTEPSELVRGLDPKSKRLSSGRSNVTAEPEVIHPQLPLS 574
           SIMTPSR+SGGDLDTCSSTHASTEPSELVRGLDPKSKRLSSGRSN+TAEPEVIHPQLPLS
Sbjct: 506 SIMTPSRISGGDLDTCSSTHASTEPSELVRGLDPKSKRLSSGRSNITAEPEVIHPQLPLS 565

Query: 575 TTPNWPRTRSKSRNDKGWTGLTTTHDTSKWGNTTTNDREDISSTLSDPGPIWDAEPKWDA 634
           TTPNWPRTRSKSRND+GW GLTTTHDTSKWGN   NDREDISSTLSDPGPIWDAEPKWD 
Sbjct: 566 TTPNWPRTRSKSRNDRGWAGLTTTHDTSKWGN-AANDREDISSTLSDPGPIWDAEPKWDP 624

Query: 635 EPTNWDVENPIELPGPSDDAEVGSTKEVVPHFGDKWVVSKGQFLGILVCNHACRTVQSSQ 694
           E  NWDVENPIELPGP DD EVGSTKEVVP FG+KWVVSKGQF+GILVCNHACRTVQ SQ
Sbjct: 625 E-ANWDVENPIELPGPPDDTEVGSTKEVVPRFGEKWVVSKGQFVGILVCNHACRTVQGSQ 683

Query: 695 VVAPKAEHDDNTLDLVLVHGNGRLKLIRFFVLLQMGRHLSLPYVENIKV 743
           VVAPKAEHDDNTLDL++VHGNGRL+L+RFF+LLQMG+H+SLPYVE +K 
Sbjct: 684 VVAPKAEHDDNTLDLIMVHGNGRLRLLRFFILLQMGQHVSLPYVEYVKA 732


>Medtr2g011820.1 | D-erythro-sphingosine kinase/diacylglycerol
           kinase | HC | chr2:2972958-2979099 | 20130731
          Length = 484

 Score = 67.0 bits (162), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 57/216 (26%), Positives = 95/216 (43%), Gaps = 8/216 (3%)

Query: 267 ELIFRCKTPPRMLVILNPRSGRGRSSKVFHGIVEPIFKLAGFRLEVVKTTSAGHARSLAS 326
           E I     P ++LV +NP  G+  + K+F   V+P+F  A  +L + +T    HA+ +A 
Sbjct: 98  EFIDSLGRPKKLLVFVNPFGGKKCAVKIFAEQVKPLFDDAQIQLTIQETKHQLHAKEVAR 157

Query: 327 SVDISTCPXXXXXXXXXXXXNEVLNGLLSRDNQKEXXXXXXXXXXXXXDNSLVWTVL-GV 385
           S+DIS                EV+NGLL R++                 N +  ++L  V
Sbjct: 158 SLDISKHDGIVCISGDGILV-EVVNGLLQREDWDTAIKTPLGIVPAGTGNGMAKSLLDSV 216

Query: 386 RDPVS---AAIAIVKGGLTATDVFAVEWAQTNKVHFGLTVSYYGFVGDVLELSEKYQKRF 442
            DP +   A +AI++G     DV  +   +T    F + +  +G + D+   SEKY +  
Sbjct: 217 GDPCAIANAVLAIIRGHKRQLDVATITQGETR--FFSILMLAWGLIADIDIESEKY-RWM 273

Query: 443 GPLRYFVAGFLKFLCLPRYSYEIEYLPASKTEREGK 478
           G  R       +   L  Y+  + ++PA   E  G+
Sbjct: 274 GSARLDFYAVCRLFNLRLYNGSVSFVPAPGFESYGE 309


>Medtr3g008260.1 | ceramide kinase-like protein | HC |
           chr3:1389057-1396955 | 20130731
          Length = 618

 Score = 59.7 bits (143), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 39/112 (34%), Positives = 57/112 (50%), Gaps = 8/112 (7%)

Query: 376 NSLVWTVLGVRDPVSAAIAIVKGGLTATDVF-AVEWAQTNK------VHFGLTVSYYGFV 428
           +++V    G RDP+++A+ IV G     D+   V W +T +      V +  + S YGF 
Sbjct: 334 DAIVICTTGARDPITSALHIVLGKRVHLDIAQVVRWKKTPRSEVEPLVRYAASFSGYGFY 393

Query: 429 GDVLELSEKYQKRFGPLRYFVAGFLKFLCLPRYSYEIEYLPASKTEREGKLS 480
           GDV++ SEKY +  GP RY  AG + FL    Y  EI +L     E +   S
Sbjct: 394 GDVIKESEKY-RWMGPKRYDYAGTVVFLRHRSYEAEISFLDDESEETDSTTS 444


>Medtr3g008260.2 | ceramide kinase-like protein | HC |
           chr3:1389057-1396955 | 20130731
          Length = 504

 Score = 59.3 bits (142), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 39/112 (34%), Positives = 57/112 (50%), Gaps = 8/112 (7%)

Query: 376 NSLVWTVLGVRDPVSAAIAIVKGGLTATDVF-AVEWAQTNK------VHFGLTVSYYGFV 428
           +++V    G RDP+++A+ IV G     D+   V W +T +      V +  + S YGF 
Sbjct: 220 DAIVICTTGARDPITSALHIVLGKRVHLDIAQVVRWKKTPRSEVEPLVRYAASFSGYGFY 279

Query: 429 GDVLELSEKYQKRFGPLRYFVAGFLKFLCLPRYSYEIEYLPASKTEREGKLS 480
           GDV++ SEKY +  GP RY  AG + FL    Y  EI +L     E +   S
Sbjct: 280 GDVIKESEKY-RWMGPKRYDYAGTVVFLRHRSYEAEISFLDDESEETDSTTS 330