Miyakogusa Predicted Gene

Lj2g3v0286840.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v0286840.1 Non Characterized Hit- tr|I3S3C3|I3S3C3_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,100,0,no
description,Cytochrome b5; Cyt-b5,Cytochrome b5; MEMBRANE STEROID
BINDING PROTEIN,NULL; MEMBRANE-,CUFF.34479.1
         (228 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr6g054890.1 | cytochrome b5-like heme/steroid-binding domain...   277   7e-75
Medtr6g054890.2 | cytochrome b5-like heme/steroid-binding domain...   277   7e-75
Medtr1g100727.1 | cytochrome b5-like heme/steroid-binding domain...   248   4e-66
Medtr6g027160.2 | cytochrome b5-like heme/steroid-binding domain...   136   1e-32
Medtr6g027160.1 | cytochrome b5-like heme/steroid-binding domain...   136   1e-32
Medtr6g027160.3 | cytochrome b5-like heme/steroid-binding domain...   136   1e-32
Medtr3g105860.1 | cytochrome b5-like heme/steroid-binding domain...   112   2e-25
Medtr3g081290.1 | cytochrome b5-like heme/steroid-binding domain...    77   1e-14
Medtr6g027060.1 | membrane steroid-binding protein, putative | H...    63   2e-10

>Medtr6g054890.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:19600682-19605989 | 20130731
          Length = 235

 Score =  277 bits (708), Expect = 7e-75,   Method: Compositional matrix adjust.
 Identities = 145/234 (61%), Positives = 159/234 (67%), Gaps = 6/234 (2%)

Query: 1   MALELWSTLKEAIVAYTGFSPXXXXXXXXXXXXXYYVLSGLFGSSDTHNRHGTTRDFEPQ 60
           MAL+LWST KEAIV YTG SP             YY+++ LFGSSDTH RHG+TRDF  +
Sbjct: 1   MALQLWSTFKEAIVVYTGLSPTTFFTLLVILFTLYYIITSLFGSSDTHQRHGSTRDFAAE 60

Query: 61  MEPLRPPVQIGEVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK 120
           MEPL+PPVQIGEVTE+ELK YDG++P+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK
Sbjct: 61  MEPLKPPVQIGEVTEDELKDYDGNNPDKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK 120

Query: 121 DASRALAKMSFEEKDLTGDISGLGSFELDALQDWEYKFMSXXXXXXXXXXXXXXXXXXX- 179
           DASRALAKMSFEEKDLTGDISGLG FEL+ALQDWEYKFM                     
Sbjct: 121 DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMGKYVKVGTIKTVPVTKPESTG 180

Query: 180 ----XXXXXXXXXXXXXXXXXXXXXSKPTENGPSETEAVKSDE-TPSNIDAAKE 228
                                    SKP EN PSET  VKSDE TP ++DA KE
Sbjct: 181 EPSESTSRGVDASSIPHENHDAAEASKPHENTPSETAPVKSDENTPLSVDADKE 234


>Medtr6g054890.2 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:19600690-19606711 | 20130731
          Length = 235

 Score =  277 bits (708), Expect = 7e-75,   Method: Compositional matrix adjust.
 Identities = 145/234 (61%), Positives = 159/234 (67%), Gaps = 6/234 (2%)

Query: 1   MALELWSTLKEAIVAYTGFSPXXXXXXXXXXXXXYYVLSGLFGSSDTHNRHGTTRDFEPQ 60
           MAL+LWST KEAIV YTG SP             YY+++ LFGSSDTH RHG+TRDF  +
Sbjct: 1   MALQLWSTFKEAIVVYTGLSPTTFFTLLVILFTLYYIITSLFGSSDTHQRHGSTRDFAAE 60

Query: 61  MEPLRPPVQIGEVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK 120
           MEPL+PPVQIGEVTE+ELK YDG++P+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK
Sbjct: 61  MEPLKPPVQIGEVTEDELKDYDGNNPDKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGK 120

Query: 121 DASRALAKMSFEEKDLTGDISGLGSFELDALQDWEYKFMSXXXXXXXXXXXXXXXXXXX- 179
           DASRALAKMSFEEKDLTGDISGLG FEL+ALQDWEYKFM                     
Sbjct: 121 DASRALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMGKYVKVGTIKTVPVTKPESTG 180

Query: 180 ----XXXXXXXXXXXXXXXXXXXXXSKPTENGPSETEAVKSDE-TPSNIDAAKE 228
                                    SKP EN PSET  VKSDE TP ++DA KE
Sbjct: 181 EPSESTSRGVDASSIPHENHDAAEASKPHENTPSETAPVKSDENTPLSVDADKE 234


>Medtr1g100727.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr1:45659938-45662838 | 20130731
          Length = 220

 Score =  248 bits (633), Expect = 4e-66,   Method: Compositional matrix adjust.
 Identities = 135/227 (59%), Positives = 154/227 (67%), Gaps = 11/227 (4%)

Query: 3   LELWSTLKEAIVAYTGFSPXXXXXXXXXXXXXYYVLSGLFGSSDTHNRHGTTRDF-EPQM 61
           ++LW TLKE+IVAYTG SP             YYVLSGLFG S + + H  TR+F E ++
Sbjct: 4   VQLWETLKESIVAYTGLSPTTFFTILALLFAVYYVLSGLFGYS-SSDLHPQTRNFQEEEL 62

Query: 62  EPLRPPVQIGEVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 121
            PLRPPVQ+GE+TEEELK+YDG+D +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD
Sbjct: 63  PPLRPPVQLGEITEEELKAYDGNDHDKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 122

Query: 122 ASRALAKMSFEEKDLTGDISGLGSFELDALQDWEYKFMSXXXXXXXXXXXXXXXXXXXXX 181
           ASRALAKMSF+EKDLTGDISGLG FELDALQDWEYKFM                      
Sbjct: 123 ASRALAKMSFDEKDLTGDISGLGPFELDALQDWEYKFMEKYVKVGTIKKEATEAESTGEP 182

Query: 182 XXXXXXXXXXXXXXXXXXXSKPTENGPSETEAVKSDETPSNIDAAKE 228
                                PTE+G S+  AVKS+ETPSN++A KE
Sbjct: 183 SGSTPRDVDVI---------NPTEDGRSKPAAVKSEETPSNVEADKE 220


>Medtr6g027160.2 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:9196689-9192831 | 20130731
          Length = 195

 Score =  136 bits (343), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 68/149 (45%), Positives = 95/149 (63%), Gaps = 8/149 (5%)

Query: 18  GFSPXXXXXXXXXXXXXYYVLSGLFGSSDTHN-------RHGTTRDFEPQMEPLRPPVQI 70
           G SP             Y  ++ +F S    N       R G++R F    EP + PVQ+
Sbjct: 17  GLSPTAFFTITLLSILVYRTVTSMFVSPQDFNKPPVVSARFGSSR-FNDVTEPPKKPVQV 75

Query: 71  GEVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMS 130
           GE++E+EL+ Y+GSD  KP+L+++KG IYDVSQ + FYGPGG YA+FAGK+ SRALA +S
Sbjct: 76  GEISEKELRLYNGSDENKPILISVKGNIYDVSQGKNFYGPGGSYAMFAGKECSRALALLS 135

Query: 131 FEEKDLTGDISGLGSFELDALQDWEYKFM 159
           F+ +D+ G++ GL   EL  L+DWEYKF+
Sbjct: 136 FKPQDINGNLEGLDESELAILEDWEYKFI 164


>Medtr6g027160.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:9196455-9193480 | 20130731
          Length = 195

 Score =  136 bits (343), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 68/149 (45%), Positives = 95/149 (63%), Gaps = 8/149 (5%)

Query: 18  GFSPXXXXXXXXXXXXXYYVLSGLFGSSDTHN-------RHGTTRDFEPQMEPLRPPVQI 70
           G SP             Y  ++ +F S    N       R G++R F    EP + PVQ+
Sbjct: 17  GLSPTAFFTITLLSILVYRTVTSMFVSPQDFNKPPVVSARFGSSR-FNDVTEPPKKPVQV 75

Query: 71  GEVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMS 130
           GE++E+EL+ Y+GSD  KP+L+++KG IYDVSQ + FYGPGG YA+FAGK+ SRALA +S
Sbjct: 76  GEISEKELRLYNGSDENKPILISVKGNIYDVSQGKNFYGPGGSYAMFAGKECSRALALLS 135

Query: 131 FEEKDLTGDISGLGSFELDALQDWEYKFM 159
           F+ +D+ G++ GL   EL  L+DWEYKF+
Sbjct: 136 FKPQDINGNLEGLDESELAILEDWEYKFI 164


>Medtr6g027160.3 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr6:9196689-9192210 | 20130731
          Length = 195

 Score =  136 bits (343), Expect = 1e-32,   Method: Compositional matrix adjust.
 Identities = 68/149 (45%), Positives = 95/149 (63%), Gaps = 8/149 (5%)

Query: 18  GFSPXXXXXXXXXXXXXYYVLSGLFGSSDTHN-------RHGTTRDFEPQMEPLRPPVQI 70
           G SP             Y  ++ +F S    N       R G++R F    EP + PVQ+
Sbjct: 17  GLSPTAFFTITLLSILVYRTVTSMFVSPQDFNKPPVVSARFGSSR-FNDVTEPPKKPVQV 75

Query: 71  GEVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMS 130
           GE++E+EL+ Y+GSD  KP+L+++KG IYDVSQ + FYGPGG YA+FAGK+ SRALA +S
Sbjct: 76  GEISEKELRLYNGSDENKPILISVKGNIYDVSQGKNFYGPGGSYAMFAGKECSRALALLS 135

Query: 131 FEEKDLTGDISGLGSFELDALQDWEYKFM 159
           F+ +D+ G++ GL   EL  L+DWEYKF+
Sbjct: 136 FKPQDINGNLEGLDESELAILEDWEYKFI 164


>Medtr3g105860.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr3:48826866-48827824 | 20130731
          Length = 100

 Score =  112 bits (281), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 49/89 (55%), Positives = 68/89 (76%)

Query: 72  EVTEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 131
           E+T ++L  Y+G+DP KP+ +A+KG++YDV+  + FYGPGG YA+FAGKDASRALAKMS 
Sbjct: 2   EMTAQQLSEYNGTDPSKPIYVAVKGRVYDVTTGKSFYGPGGAYAMFAGKDASRALAKMSK 61

Query: 132 EEKDLTGDISGLGSFELDALQDWEYKFMS 160
            E+D+T  + GL   E+  L DWE KF++
Sbjct: 62  NEEDITSSLDGLTEKEIGVLNDWETKFVA 90


>Medtr3g081290.1 | cytochrome b5-like heme/steroid-binding domain
           protein | HC | chr3:36822514-36819099 | 20130731
          Length = 238

 Score = 77.4 bits (189), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 36/82 (43%), Positives = 52/82 (63%)

Query: 74  TEEELKSYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEE 133
           T EEL  ++G+DP  P+L+ I G ++DV++ +  YGPGG Y  FAG+DASRA    +F  
Sbjct: 36  TTEELALFNGTDPGLPILIGILGSVFDVTKGKSHYGPGGGYHHFAGRDASRAFFSGNFTG 95

Query: 134 KDLTGDISGLGSFELDALQDWE 155
             LT  + GL S E+ ++ DW 
Sbjct: 96  DGLTDSLRGLSSTEVKSVVDWR 117


>Medtr6g027060.1 | membrane steroid-binding protein, putative | HC |
           chr6:9163853-9164183 | 20130731
          Length = 86

 Score = 63.2 bits (152), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 27/53 (50%), Positives = 38/53 (71%)

Query: 107 FYGPGGPYALFAGKDASRALAKMSFEEKDLTGDISGLGSFELDALQDWEYKFM 159
           FYG GG YA+FAGK+ +  LA +SF+ +D+ G++ G    EL  L+DWEYKF+
Sbjct: 27  FYGSGGLYAMFAGKECNLPLALLSFKPQDINGNLEGFHKSELTVLEDWEYKFI 79