Miyakogusa Predicted Gene

Lj2g3v0126210.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj2g3v0126210.1 tr|Q9MB08|Q9MB08_HELAN Multicystatin
OS=Helianthus annuus GN=smc PE=2
SV=1,56.32,4e-17,Cystatin/monellin,NULL; Cystatin-like
domain,Proteinase inhibitor I25, cystatin;
CYSTATIN,Proteinase,NODE_17764_length_320_cov_1150.887451.path1.1
         (109 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g041410.1 | cysteine protease inhibitor cystatin | HC | ch...   172   7e-44
Medtr3g043750.1 | cysteine protease inhibitor cystatin | HC | ch...   140   3e-34
Medtr3g043750.2 | cysteine protease inhibitor cystatin | HC | ch...   140   3e-34
Medtr5g088770.1 | cysteine protease inhibitor cystatin | HC | ch...   112   1e-25
Medtr3g452280.1 | cysteine protease inhibitor cystatin | LC | ch...    90   4e-19
Medtr3g451210.1 | cysteine protease inhibitor cystatin | LC | ch...    90   4e-19
Medtr4g133540.1 | phloem filament protein PP1 | HC | chr4:558655...    67   4e-12
Medtr0526s0020.1 | cysteine protease inhibitor cystatin | LC | s...    65   2e-11
Medtr2g026040.1 | phloem filament protein PP1 | HC | chr2:938688...    62   1e-10
Medtr8g098380.1 | cysteine proteinase inhibitor, putative | LC |...    54   4e-08
Medtr2g103740.1 | cysteine proteinase inhibitor | LC | chr2:4466...    53   8e-08
Medtr3g451850.1 | cysteine protease inhibitor cystatin | LC | ch...    51   2e-07
Medtr4g017620.1 | phloem filament protein PP1 | HC | chr4:552059...    50   4e-07
Medtr3g084780.1 | phloem filament protein PP1 | HC | chr3:382844...    49   1e-06
Medtr8g080920.1 | cysteine protease inhibitor cystatin | HC | ch...    48   2e-06

>Medtr8g041410.1 | cysteine protease inhibitor cystatin | HC |
           chr8:15583939-15587672 | 20130731
          Length = 241

 Score =  172 bits (435), Expect = 7e-44,   Method: Compositional matrix adjust.
 Identities = 84/110 (76%), Positives = 94/110 (85%), Gaps = 1/110 (0%)

Query: 1   MATKLGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLT 60
           MAT LGGI+DS   +NSLEI++LARFAVD+HN KQN+LLEF RVVKA+EQVVAG++HHLT
Sbjct: 38  MATPLGGIQDSPSSENSLEIESLARFAVDQHNAKQNSLLEFARVVKAREQVVAGTLHHLT 97

Query: 61  LEAIDGGEKKIYEAKVWVKSWLNFKELQEFKEVA-GDAPLFTTSDLGVKK 109
           LEAID GEKKIYEAKVWVK W+NFKEL EFK    G AP FTTSDLGV K
Sbjct: 98  LEAIDAGEKKIYEAKVWVKPWMNFKELTEFKHAGDGHAPSFTTSDLGVIK 147


>Medtr3g043750.1 | cysteine protease inhibitor cystatin | HC |
          chr3:14747264-14750798 | 20130731
          Length = 98

 Score =  140 bits (353), Expect = 3e-34,   Method: Compositional matrix adjust.
 Identities = 70/98 (71%), Positives = 81/98 (82%), Gaps = 2/98 (2%)

Query: 1  MATKLGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLT 60
          MAT +GG+RD  G QNSL ID LARFAV+EHN KQNALLEF RV+ A+EQVVAG++HH+T
Sbjct: 1  MAT-VGGVRDVSGNQNSLAIDGLARFAVEEHNKKQNALLEFSRVISAKEQVVAGTIHHIT 59

Query: 61 LEAIDGGEKKIYEAKVWVKSWLNFKELQEFKEVAGDAP 98
          LE  DG  KK+YEAKVW KSW+NFKE+QEFK V  DAP
Sbjct: 60 LEVKDGVNKKVYEAKVWEKSWMNFKEVQEFKLVE-DAP 96


>Medtr3g043750.2 | cysteine protease inhibitor cystatin | HC |
          chr3:14747266-14750798 | 20130731
          Length = 98

 Score =  140 bits (353), Expect = 3e-34,   Method: Compositional matrix adjust.
 Identities = 70/98 (71%), Positives = 81/98 (82%), Gaps = 2/98 (2%)

Query: 1  MATKLGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLT 60
          MAT +GG+RD  G QNSL ID LARFAV+EHN KQNALLEF RV+ A+EQVVAG++HH+T
Sbjct: 1  MAT-VGGVRDVSGNQNSLAIDGLARFAVEEHNKKQNALLEFSRVISAKEQVVAGTIHHIT 59

Query: 61 LEAIDGGEKKIYEAKVWVKSWLNFKELQEFKEVAGDAP 98
          LE  DG  KK+YEAKVW KSW+NFKE+QEFK V  DAP
Sbjct: 60 LEVKDGVNKKVYEAKVWEKSWMNFKEVQEFKLVE-DAP 96


>Medtr5g088770.1 | cysteine protease inhibitor cystatin | HC |
           chr5:38553862-38552423 | 20130731
          Length = 104

 Score =  112 bits (279), Expect = 1e-25,   Method: Compositional matrix adjust.
 Identities = 55/101 (54%), Positives = 78/101 (77%), Gaps = 2/101 (1%)

Query: 5   LGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAI 64
           LGG  + +  QNS+EID LA FAV EHN KQNA+LEF RV+ A+++VV+G+++ +TLE  
Sbjct: 4   LGGSTEVEYTQNSVEIDNLAIFAVQEHNIKQNAVLEFVRVLNAKKKVVSGTLYDITLETK 63

Query: 65  DGGEKKIYEAKVWVKSWLNFKELQEFKEVA--GDAPLFTTS 103
           DGG++K+YEAK+  K WLNFKE+QEFK ++   DAP  +++
Sbjct: 64  DGGKQKVYEAKILEKPWLNFKEVQEFKLISQNDDAPSVSST 104


>Medtr3g452280.1 | cysteine protease inhibitor cystatin | LC |
           chr3:19075194-19075900 | 20130731
          Length = 139

 Score = 90.1 bits (222), Expect = 4e-19,   Method: Compositional matrix adjust.
 Identities = 44/93 (47%), Positives = 62/93 (66%)

Query: 5   LGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAI 64
            GGI +  G QN   ID LARFAVD++N K     EF +VV A++++VAG ++++TLEA 
Sbjct: 31  CGGITEINGSQNCPTIDGLARFAVDQNNKKVVPKKEFVKVVSAKQKLVAGMLYYITLEAK 90

Query: 65  DGGEKKIYEAKVWVKSWLNFKELQEFKEVAGDA 97
            G +  +YEA++W + WL+ KEL EFK V  D 
Sbjct: 91  VGEKVNVYEAQIWDRPWLDLKELTEFKRVDDDP 123


>Medtr3g451210.1 | cysteine protease inhibitor cystatin | LC |
           chr3:17773893-17774599 | 20130731
          Length = 139

 Score = 90.1 bits (222), Expect = 4e-19,   Method: Compositional matrix adjust.
 Identities = 44/93 (47%), Positives = 62/93 (66%)

Query: 5   LGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAI 64
            GGI +  G QN   ID LARFAVD++N K     EF +VV A++++VAG ++++TLEA 
Sbjct: 31  CGGITEINGSQNCPTIDGLARFAVDQNNKKVVPKKEFVKVVSAKQKLVAGMLYYITLEAK 90

Query: 65  DGGEKKIYEAKVWVKSWLNFKELQEFKEVAGDA 97
            G +  +YEA++W + WL+ KEL EFK V  D 
Sbjct: 91  VGEKVNVYEAQIWDRPWLDLKELTEFKRVDDDP 123


>Medtr4g133540.1 | phloem filament protein PP1 | HC |
           chr4:55865577-55865930 | 20130731
          Length = 117

 Score = 67.0 bits (162), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 33/78 (42%), Positives = 46/78 (58%)

Query: 16  NSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAK 75
           N   +  +A FAV E++ +  A L+F +V+  + QVVAG+ + LTL A DG   K YEA 
Sbjct: 38  NDPHVTEIANFAVTEYDRRSGAKLKFEKVINGESQVVAGTNYRLTLSASDGSYSKNYEAV 97

Query: 76  VWVKSWLNFKELQEFKEV 93
           VW K W +F+ L  F  V
Sbjct: 98  VWEKIWQHFRNLTSFVPV 115


>Medtr0526s0020.1 | cysteine protease inhibitor cystatin | LC |
          scaffold0526:8773-6588 | 20130731
          Length = 266

 Score = 64.7 bits (156), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 31/72 (43%), Positives = 45/72 (62%)

Query: 20 IDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAKVWVK 79
          I  L  FAVD++N K+ A L+F  VV     +V G  + +TL+A DG +   Y+A +WV+
Sbjct: 28 ISCLGSFAVDKYNKKEKANLKFVEVVDVNSYMVEGISYDVTLKAKDGEKMNFYKANIWVR 87

Query: 80 SWLNFKELQEFK 91
           WLN +EL EF+
Sbjct: 88 PWLNKEELTEFR 99



 Score = 49.7 bits (117), Expect = 5e-07,   Method: Compositional matrix adjust.
 Identities = 23/54 (42%), Positives = 36/54 (66%)

Query: 23  LARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAKV 76
           LA FA+DE+N K+N  L+F + +  +   V G ++H+TLEA DG    +Y+A+V
Sbjct: 108 LACFAIDEYNKKENGNLKFVKCLYFKMYSVVGELYHITLEAKDGENVNVYKAEV 161


>Medtr2g026040.1 | phloem filament protein PP1 | HC |
           chr2:9386884-9388536 | 20130731
          Length = 117

 Score = 61.6 bits (148), Expect = 1e-10,   Method: Compositional matrix adjust.
 Identities = 34/92 (36%), Positives = 50/92 (54%), Gaps = 7/92 (7%)

Query: 3   TKLGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLE 62
           T +  ++D Q       +  +A+FAV E+  +  + L F +V+K + QVV+G+ + L L 
Sbjct: 33  TPIKNVKDPQ-------VVEIAKFAVTEYGKQSGSKLSFVKVIKGETQVVSGTNYRLVLA 85

Query: 63  AIDGGEKKIYEAKVWVKSWLNFKELQEFKEVA 94
           A      K YEA VW K WL+ K L  FK VA
Sbjct: 86  AKVVSATKNYEALVWEKPWLHLKNLTSFKPVA 117


>Medtr8g098380.1 | cysteine proteinase inhibitor, putative | LC |
           chr8:40901302-40902847 | 20130731
          Length = 223

 Score = 53.5 bits (127), Expect = 4e-08,   Method: Compositional matrix adjust.
 Identities = 28/85 (32%), Positives = 47/85 (55%)

Query: 20  IDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAKVWVK 79
           I+  A   V  HN K+NA L+F  VV A+ +      +H+TL+A DG    +Y+  ++V+
Sbjct: 73  INIFALLLVHNHNKKENANLKFVNVVNAEFRRCGAMGYHITLKAKDGENVNVYDTGIFVE 132

Query: 80  SWLNFKELQEFKEVAGDAPLFTTSD 104
           +W+++KE+    E+      F  SD
Sbjct: 133 AWMDWKEMLILHELTLSHNQFLASD 157


>Medtr2g103740.1 | cysteine proteinase inhibitor | LC |
           chr2:44664882-44664544 | 20130731
          Length = 112

 Score = 52.8 bits (125), Expect = 8e-08,   Method: Compositional matrix adjust.
 Identities = 29/81 (35%), Positives = 42/81 (51%), Gaps = 1/81 (1%)

Query: 1   MATKLGGIRDSQGFQNSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLT 60
           M T  GG    +   N   +  +A FAV E+N    A L+  +++K + QV +G  + L 
Sbjct: 21  METSAGGWSPIKDI-NDPHVIVIANFAVTEYNKHTGANLKLDKLIKGESQVTSGIYYDLI 79

Query: 61  LEAIDGGEKKIYEAKVWVKSW 81
           L A DG    IY+A VW K+W
Sbjct: 80  LSAGDGSHSNIYKALVWEKTW 100


>Medtr3g451850.1 | cysteine protease inhibitor cystatin | LC |
           chr3:18858607-18861064 | 20130731
          Length = 306

 Score = 51.2 bits (121), Expect = 2e-07,   Method: Compositional matrix adjust.
 Identities = 33/83 (39%), Positives = 50/83 (60%), Gaps = 10/83 (12%)

Query: 21  DALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAKV---- 76
           + L R AV+ +N K+NA L+F + +  +   VAG ++H+TLEA DG +  +Y+A+V    
Sbjct: 78  NGLPRHAVNRYNKKENANLKFVKCLHFKRYFVAGELYHITLEAKDGEKVNVYKAEVRERL 137

Query: 77  -WVKSWLNFKELQEFKEVAGDAP 98
              K +L    L EFK +A DAP
Sbjct: 138 YQQKGFL----LTEFK-LADDAP 155



 Score = 48.9 bits (115), Expect = 9e-07,   Method: Compositional matrix adjust.
 Identities = 29/80 (36%), Positives = 48/80 (60%), Gaps = 10/80 (12%)

Query: 21  DALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKKIYEAKVWVKS 80
           D LARFAVD HN      +E    + +++ + +G  + +TLEA DG +  +Y+A++  + 
Sbjct: 236 DNLARFAVDLHN------IEHKEGIDSKQDLSSGYFYDVTLEAKDGEKVNVYQARIRERP 289

Query: 81  WLNFKELQEFKEVAGDAPLF 100
           W   ++L EFK + G+APL 
Sbjct: 290 W---QQLWEFK-LVGEAPLL 305


>Medtr4g017620.1 | phloem filament protein PP1 | HC |
           chr4:5520592-5521077 | 20130731
          Length = 116

 Score = 50.1 bits (118), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 32/81 (39%), Positives = 49/81 (60%), Gaps = 3/81 (3%)

Query: 16  NSLEIDALARFAVDEHNNKQNALLEFGRVVKAQEQVVAGSMHHLTLEAIDGGEKK--IYE 73
           N   +  +A+FAV E++ ++   L+F +VVK + QVVAG+ + LT+ AI G + K   YE
Sbjct: 34  NDPHVIEIAKFAVTEYDKQKGTDLKFEKVVKGESQVVAGTNYRLTISAIHGSDSKSNSYE 93

Query: 74  AKVWVK-SWLNFKELQEFKEV 93
           A V+ K S  + K+L  F  V
Sbjct: 94  AIVYEKPSSEHLKKLISFIPV 114


>Medtr3g084780.1 | phloem filament protein PP1 | HC |
           chr3:38284448-38286450 | 20130731
          Length = 130

 Score = 48.9 bits (115), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 28/86 (32%), Positives = 52/86 (60%), Gaps = 9/86 (10%)

Query: 19  EIDALARFAVDEHNNKQNALL-------EFGRVVKAQEQVVAGSMHHLTLEAID-GGEKK 70
           E+  L +FAV E+N KQ           +F  VV+A++QVV+G  ++L + A+D  G ++
Sbjct: 41  EVQELGKFAVKEYNYKQGLNNGGGGEGLKFVEVVEAEQQVVSGMKYYLNISAVDHNGVQR 100

Query: 71  IYEAKVWVKSWLN-FKELQEFKEVAG 95
           ++ + V VK WL+ +K++ + + + G
Sbjct: 101 MFNSVVVVKPWLHQYKKVIQTRHIMG 126


>Medtr8g080920.1 | cysteine protease inhibitor cystatin | HC |
           chr8:34953682-34953281 | 20130731
          Length = 133

 Score = 48.1 bits (113), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 32/97 (32%), Positives = 51/97 (52%), Gaps = 11/97 (11%)

Query: 5   LGGIRDSQGFQNSLEIDALARFAVDEHNNKQNAL------LEFGRVVKAQEQVVAGSMHH 58
           +GG  +    + + E+  L RFAV+E+N            L F  VV+AQ+QVV+G  ++
Sbjct: 32  VGGKTEVANVRTNDEVQELGRFAVEEYNRSVKIWKEGEGELRFVEVVEAQQQVVSGIKYY 91

Query: 59  LTLEAI----DG-GEKKIYEAKVWVKSWLNFKELQEF 90
           + +       DG G+  ++++ V VK WLN K L  F
Sbjct: 92  MKIWVTQAKSDGVGDPTMFDSVVLVKPWLNSKHLLHF 128