Miyakogusa Predicted Gene

Lj1g3v5061070.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v5061070.1 Non Characterized Hit- tr|J9JB62|J9JB62_9SPIT
Membrane protein, putative OS=Oxytricha trifallax
PE=4,33.49,9e-19,S5A_REDUCTASE,3-oxo-5-alpha-steroid 4-dehydrogenase,
C-terminal; DUF1295,Protein of unknown
function,NODE_31073_length_1833_cov_57.566830.path1.1
         (332 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g023760.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...   497   e-141
Medtr7g117475.1 | phospholipid methyltransferase | HC | chr7:486...   434   e-122
Medtr8g107030.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    68   1e-11
Medtr8g107030.2 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    67   2e-11
Medtr8g107030.3 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC | c...    63   4e-10

>Medtr8g023760.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:8635853-8638859 | 20130731
          Length = 329

 Score =  497 bits (1280), Expect = e-141,   Method: Compositional matrix adjust.
 Identities = 232/324 (71%), Positives = 270/324 (83%)

Query: 8   SNMKNAILAFLVPLPSILFYLSFLSATNGSVADPKSPSFTSTVWTWCYHYPXXXXXXXXX 67
            N+KNAI+A LVPLPSI+FYL+FL+  + S++ P +PSF ST+WTWCYH+P         
Sbjct: 5   QNLKNAIIAILVPLPSIIFYLTFLNNYHSSISIPNNPSFFSTLWTWCYHHPLLFANALFF 64

Query: 68  XXXXXXXWVIGLIQSSHWMIDPYWTVIPVMLVHYYSAHPLAQYHCWRSRIVILLTWVWSI 127
                  W+IG IQSSHW+IDPYWTVIPVMLVHYY+ HPLAQY  WRS+IVILLTWVWSI
Sbjct: 65  FNVNIIFWLIGQIQSSHWLIDPYWTVIPVMLVHYYATHPLAQYDLWRSKIVILLTWVWSI 124

Query: 128 RLIHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYVIH 187
           RL HNYFRREKWQWG REDWRFT MS QYG  WWWVSFF++YV QQ+FL+GLSLP YV+H
Sbjct: 125 RLTHNYFRREKWQWGAREDWRFTQMSQQYGKLWWWVSFFAVYVSQQIFLIGLSLPLYVVH 184

Query: 188 SVNQPLSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWYYS 247
            VN+PLSI DL+A +VC+SGIVIAYFADTQLH+F+SRNN+LKG GK V+PVLD GLWYYS
Sbjct: 185 FVNKPLSILDLVAIVVCLSGIVIAYFADTQLHDFMSRNNQLKGLGKPVIPVLDTGLWYYS 244

Query: 248 RHPNYFGEQLWWWGLVVFTWNLGHGWTVIGALANTMCLAYVTKLVENRMLSQDNRAEAYR 307
           R PNYFGE LWWWGLVVF WNLGHGWT IGAL NT CLAYV +LVE+RML Q++RAEA++
Sbjct: 245 RRPNYFGETLWWWGLVVFAWNLGHGWTFIGALVNTFCLAYVARLVEDRMLKQESRAEAFK 304

Query: 308 LYQRTTSVWVPWFKSSPLGLKSKN 331
           LYQ+TTS W+PWFKS P  +K+KN
Sbjct: 305 LYQKTTSAWIPWFKSFPSDVKNKN 328


>Medtr7g117475.1 | phospholipid methyltransferase | HC |
           chr7:48618001-48616890 | 20130731
          Length = 314

 Score =  434 bits (1115), Expect = e-122,   Method: Compositional matrix adjust.
 Identities = 217/316 (68%), Positives = 240/316 (75%), Gaps = 21/316 (6%)

Query: 6   AVSNMKNAILAFLVPLPSILFYLSFLSATNGSVADPKSPSFTSTVWTWCYHYPXXXXXXX 65
             SN+KNA++AFL PLPSI+FYLSF+   N +   P           +CY +P       
Sbjct: 3   CTSNLKNAVIAFLAPLPSIIFYLSFI---NNATDHP-----------FCYRHPLLLVNLL 48

Query: 66  XXXXXXXXXWVIGLIQSSHWMIDPYWTVIPVMLVHYYSAHPLAQYHCWRSRIVILLTWVW 125
                    W+I LIQSSHWMIDPYWTVIPVMLVHYY+AHPLA Y  WRSRIVILLTWVW
Sbjct: 49  FFFNVNILFWLIALIQSSHWMIDPYWTVIPVMLVHYYAAHPLAHYDFWRSRIVILLTWVW 108

Query: 126 SIRLIHNYFRREKWQWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYV 185
           SIRLIHNYFRREKWQWG REDWRFTDM  QYG HWWW SFFSIY+P QL L+GLSLPFYV
Sbjct: 109 SIRLIHNYFRREKWQWGAREDWRFTDMIQQYGKHWWWASFFSIYLPHQLLLIGLSLPFYV 168

Query: 186 IHSVNQPLSIWDLLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWY 245
           IHSVNQPL+IWDL+A +VCVSGI+IAYFAD QL+NFV           S   +L++GLWY
Sbjct: 169 IHSVNQPLNIWDLVAVVVCVSGILIAYFADNQLYNFVM-------MMMSNDMILESGLWY 221

Query: 246 YSRHPNYFGEQLWWWGLVVFTWNLGHGWTVIGALANTMCLAYVTKLVENRMLSQDNRAEA 305
           Y+RHPNYFGEQLWWWGLVVF WNLG+GWT IGAL NTMCL YVTKLVE RML QD R EA
Sbjct: 222 YTRHPNYFGEQLWWWGLVVFGWNLGYGWTFIGALLNTMCLGYVTKLVEQRMLKQDERVEA 281

Query: 306 YRLYQRTTSVWVPWFK 321
           YR YQ TTSVW+P FK
Sbjct: 282 YRKYQNTTSVWIPCFK 297


>Medtr8g107030.1 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:45212143-45214354 | 20130731
          Length = 307

 Score = 67.8 bits (164), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 61/201 (30%), Positives = 100/201 (49%), Gaps = 33/201 (16%)

Query: 140 QWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYVIHSVNQP--LSIWD 197
           QWG  ED RF  M +  G     ++ F I+  Q +++  +SLP  V+++ ++   L   D
Sbjct: 87  QWG--EDRRFDQMRNNLGK----LAIFWIF--QAVWVWAVSLPVTVVNATDRNPFLQSVD 138

Query: 198 LLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWYYSRHPNYFGEQL 257
           L+  ++   G +I   AD Q  NF  R+ + +G+        + GLW Y+RHPNYFGE L
Sbjct: 139 LIGWLMWAVGFMIEGTADQQKLNF-KRSPENRGKW------CNAGLWKYTRHPNYFGEIL 191

Query: 258 WWWGLVVFT---WNLGHGWTVIGALANTMCLAYVT--KLVENRMLSQDNRAEAYRLYQRT 312
            WWG+ V +    +      +IG +  T+ L +++   L+E     +    + YR+Y++ 
Sbjct: 192 LWWGIFVASTPILDRAEWLVIIGPIFLTLLLLFISGIPLLEESADKKFGNVDGYRVYKQR 251

Query: 313 TSVWVP-----------WFKS 322
           TS  +P           WFKS
Sbjct: 252 TSPLIPLPPAVYGKLPTWFKS 272


>Medtr8g107030.2 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:45212143-45214354 | 20130731
          Length = 338

 Score = 67.0 bits (162), Expect = 2e-11,   Method: Compositional matrix adjust.
 Identities = 61/201 (30%), Positives = 100/201 (49%), Gaps = 33/201 (16%)

Query: 140 QWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYVIHSVNQP--LSIWD 197
           QWG  ED RF  M +  G     ++ F I+  Q +++  +SLP  V+++ ++   L   D
Sbjct: 118 QWG--EDRRFDQMRNNLGK----LAIFWIF--QAVWVWAVSLPVTVVNATDRNPFLQSVD 169

Query: 198 LLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWYYSRHPNYFGEQL 257
           L+  ++   G +I   AD Q  NF  R+ + +G+        + GLW Y+RHPNYFGE L
Sbjct: 170 LIGWLMWAVGFMIEGTADQQKLNF-KRSPENRGKW------CNAGLWKYTRHPNYFGEIL 222

Query: 258 WWWGLVVFT---WNLGHGWTVIGALANTMCLAYVT--KLVENRMLSQDNRAEAYRLYQRT 312
            WWG+ V +    +      +IG +  T+ L +++   L+E     +    + YR+Y++ 
Sbjct: 223 LWWGIFVASTPILDRAEWLVIIGPIFLTLLLLFISGIPLLEESADKKFGNVDGYRVYKQR 282

Query: 313 TSVWVP-----------WFKS 322
           TS  +P           WFKS
Sbjct: 283 TSPLIPLPPAVYGKLPTWFKS 303


>Medtr8g107030.3 | 3-oxo-5-alpha-steroid 4-dehydrogenase | HC |
           chr8:45212143-45214658 | 20130731
          Length = 253

 Score = 62.8 bits (151), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 55/181 (30%), Positives = 93/181 (51%), Gaps = 22/181 (12%)

Query: 140 QWGVREDWRFTDMSHQYGSHWWWVSFFSIYVPQQLFLMGLSLPFYVIHSVNQP--LSIWD 197
           QWG  ED RF  M +  G     ++ F I+  Q +++  +SLP  V+++ ++   L   D
Sbjct: 87  QWG--EDRRFDQMRNNLGK----LAIFWIF--QAVWVWAVSLPVTVVNATDRNPFLQSVD 138

Query: 198 LLATIVCVSGIVIAYFADTQLHNFVSRNNKLKGQGKSVVPVLDNGLWYYSRHPNYFGEQL 257
           L+  ++   G +I   AD Q  NF  R+ + +G+        + GLW Y+RHPNYFGE L
Sbjct: 139 LIGWLMWAVGFMIEGTADQQKLNF-KRSPENRGKW------CNAGLWKYTRHPNYFGEIL 191

Query: 258 WWWGLVVFT---WNLGHGWTVIGALANTMCLAYVTK--LVENRMLSQDNRAEAYRLYQRT 312
            WWG+ V +    +      +IG +  T+ L +++   L+E     +    + YR+Y++ 
Sbjct: 192 LWWGIFVASTPILDRAEWLVIIGPIFLTLLLLFISGIPLLEESADKKFGNVDGYRVYKQR 251

Query: 313 T 313
           T
Sbjct: 252 T 252