Miyakogusa Predicted Gene

Lj1g3v5034660.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v5034660.2 Non Characterized Hit- tr|I1NCK5|I1NCK5_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.9322
PE=,76.46,0,alpha/beta-Hydrolases,NULL; seg,NULL; FAMILY NOT
NAMED,NULL; no description,NULL; Lipase_3,Lipase, c,CUFF.33891.2
         (689 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g117280.1 | triacylglycerol lipase | HC | chr7:48446488-48...   934   0.0  
Medtr7g117280.2 | triacylglycerol lipase | HC | chr7:48449440-48...   934   0.0  
Medtr7g109350.1 | triacylglycerol lipase | HC | chr7:44694033-44...   353   3e-97
Medtr1g087330.1 | triacylglycerol lipase | HC | chr1:39148495-39...   306   5e-83

>Medtr7g117280.1 | triacylglycerol lipase | HC |
           chr7:48446488-48452243 | 20130731
          Length = 695

 Score =  934 bits (2413), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 475/701 (67%), Positives = 523/701 (74%), Gaps = 18/701 (2%)

Query: 1   MAYSAIAMPTSPTM----DISTEQNDLRRSHSNKDLLCTRSIMRRSYSENHLYSHINRIQ 56
           MAY+A+ +PTSPT     DI+ E+  LRRS S+ DL C RSIM+RSYS+++L    NRIQ
Sbjct: 1   MAYTAVGIPTSPTTSSTKDITKERYGLRRSRSSIDL-CRRSIMQRSYSDSYLCCSFNRIQ 59

Query: 57  ATSMQSNHK-----GNSPFQISSSIFPNSLRSFLFDSQTSKGVNIREKSFHVXXXXXXXX 111
           ATS+Q   K     G SPFQ S SI PNSLRSFLFD Q  K +N REK  ++        
Sbjct: 60  ATSVQPKLKDNGSMGTSPFQFSGSILPNSLRSFLFDQQNGKDMNKREKDVNIEENMVETS 119

Query: 112 XXXXRVDRTNWVERLMEIKKRWRNRIPKESIHMDDGACYDNTTSGXXX--XXXXXXXXXX 169
               RV+R NW+ERL+EIKK WRNR+PKES+ MD     D+ TSG               
Sbjct: 120 NEE-RVNRANWIERLVEIKKHWRNRLPKESVDMD--VMCDDYTSGECDCDDDSVCIADYD 176

Query: 170 XXXXXXXXXXXXXXSFSKFLAQVPWTDTKHYSQLAFLCNMAYVIPKIKAMDLRRYYSLQF 229
                         SFSKFL QV  +DTK YSQLAFLCNMAYVIP+IKA +LRRYYSLQF
Sbjct: 177 DEEEGGQEVTHDRDSFSKFLVQVSLSDTKLYSQLAFLCNMAYVIPQIKAQELRRYYSLQF 236

Query: 230 VTSSXXXXXXXXXXXXXXDQDSTRVPIDDSAASQDGSEKCKDNENKHQIRVAYDIAASAA 289
           +TSS               QDS   PIDD   SQ+  E+ KD E   QIR+AYDIAASAA
Sbjct: 237 ITSSLEKKAAVAKLKAKLTQDSPNSPIDDLVVSQNSLEEGKDKEQNPQIRLAYDIAASAA 296

Query: 290 SYVQLRAKDLLSLAAKSQHSDNNNKDSNG-VDSPQQEAEATSRGYKSEVAAYMAASTMTS 348
           SYVQLRAK+LL+LAAKSQ S   N+DS+G  DSP+QEAE TSR YKSEVAAYM AST+TS
Sbjct: 297 SYVQLRAKNLLTLAAKSQQS--KNEDSSGRKDSPEQEAEGTSRDYKSEVAAYMVASTVTS 354

Query: 349 VVAAGERERHETAKDLQSLHSSPCEWFVCDDFSNYTRCFVIQGSDSVASWHANLFFEPTK 408
           VVA+GERER E A  LQSLHSSPCEWFVCDDFSNYTRCFVIQGSDS+ASW ANLFFEPTK
Sbjct: 355 VVASGERERQEAATSLQSLHSSPCEWFVCDDFSNYTRCFVIQGSDSLASWQANLFFEPTK 414

Query: 409 FEGTDVLVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFTXXXXXXXXXXXXXXXXX 468
           FE TDVLVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFT                 
Sbjct: 415 FENTDVLVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFTGHSLGGSLSLLVHLMLL 474

Query: 469 ARKXXXXXXXXXXXXFGSPFVFCGGQKLMDELGMDESQIHCVMMHRDIVPRAFSCNYPNH 528
            RK            FGSPFVFCGGQKL+DELG+DE+QIHCVMMHRDIVPRAFSCNYP+H
Sbjct: 475 TRKVVSPSTLKPVVTFGSPFVFCGGQKLIDELGVDENQIHCVMMHRDIVPRAFSCNYPDH 534

Query: 529 VALVLKRLNSTFRSHPCLTKNKLLYSPLGKIFILQPDEKTSPPHPLLPPGSAFYALDSTR 588
           VA++LKRLN TFRSHPCLTKNKLLY+PLGKIFI+QPDE TSPPHPLLP  SAFY LDST 
Sbjct: 535 VAVILKRLNRTFRSHPCLTKNKLLYTPLGKIFIIQPDEMTSPPHPLLPSESAFYELDSTI 594

Query: 589 CGYSPSVLRTFLNQPHPIETLSDPTAYGSEGTILRDHDSSNYLKAVNGILRQHSKILVRR 648
           CGYSP VL +FLNQPHPIETLSDPTAYG+EGTILRDHDSSNYLKAVNGILRQHSK LVRR
Sbjct: 595 CGYSPRVLSSFLNQPHPIETLSDPTAYGAEGTILRDHDSSNYLKAVNGILRQHSKTLVRR 654

Query: 649 VREQRINELWPLLTSPSPHLWSHEQNMERCRFMTKEIITGV 689
           V++QRI+ELWPLLTSPSPH WSHEQNMERC  MT EI TGV
Sbjct: 655 VKKQRIDELWPLLTSPSPHSWSHEQNMERCILMTNEITTGV 695


>Medtr7g117280.2 | triacylglycerol lipase | HC |
           chr7:48449440-48452275 | 20130731
          Length = 695

 Score =  934 bits (2413), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 475/701 (67%), Positives = 523/701 (74%), Gaps = 18/701 (2%)

Query: 1   MAYSAIAMPTSPTM----DISTEQNDLRRSHSNKDLLCTRSIMRRSYSENHLYSHINRIQ 56
           MAY+A+ +PTSPT     DI+ E+  LRRS S+ DL C RSIM+RSYS+++L    NRIQ
Sbjct: 1   MAYTAVGIPTSPTTSSTKDITKERYGLRRSRSSIDL-CRRSIMQRSYSDSYLCCSFNRIQ 59

Query: 57  ATSMQSNHK-----GNSPFQISSSIFPNSLRSFLFDSQTSKGVNIREKSFHVXXXXXXXX 111
           ATS+Q   K     G SPFQ S SI PNSLRSFLFD Q  K +N REK  ++        
Sbjct: 60  ATSVQPKLKDNGSMGTSPFQFSGSILPNSLRSFLFDQQNGKDMNKREKDVNIEENMVETS 119

Query: 112 XXXXRVDRTNWVERLMEIKKRWRNRIPKESIHMDDGACYDNTTSGXXX--XXXXXXXXXX 169
               RV+R NW+ERL+EIKK WRNR+PKES+ MD     D+ TSG               
Sbjct: 120 NEE-RVNRANWIERLVEIKKHWRNRLPKESVDMD--VMCDDYTSGECDCDDDSVCIADYD 176

Query: 170 XXXXXXXXXXXXXXSFSKFLAQVPWTDTKHYSQLAFLCNMAYVIPKIKAMDLRRYYSLQF 229
                         SFSKFL QV  +DTK YSQLAFLCNMAYVIP+IKA +LRRYYSLQF
Sbjct: 177 DEEEGGQEVTHDRDSFSKFLVQVSLSDTKLYSQLAFLCNMAYVIPQIKAQELRRYYSLQF 236

Query: 230 VTSSXXXXXXXXXXXXXXDQDSTRVPIDDSAASQDGSEKCKDNENKHQIRVAYDIAASAA 289
           +TSS               QDS   PIDD   SQ+  E+ KD E   QIR+AYDIAASAA
Sbjct: 237 ITSSLEKKAAVAKLKAKLTQDSPNSPIDDLVVSQNSLEEGKDKEQNPQIRLAYDIAASAA 296

Query: 290 SYVQLRAKDLLSLAAKSQHSDNNNKDSNG-VDSPQQEAEATSRGYKSEVAAYMAASTMTS 348
           SYVQLRAK+LL+LAAKSQ S   N+DS+G  DSP+QEAE TSR YKSEVAAYM AST+TS
Sbjct: 297 SYVQLRAKNLLTLAAKSQQS--KNEDSSGRKDSPEQEAEGTSRDYKSEVAAYMVASTVTS 354

Query: 349 VVAAGERERHETAKDLQSLHSSPCEWFVCDDFSNYTRCFVIQGSDSVASWHANLFFEPTK 408
           VVA+GERER E A  LQSLHSSPCEWFVCDDFSNYTRCFVIQGSDS+ASW ANLFFEPTK
Sbjct: 355 VVASGERERQEAATSLQSLHSSPCEWFVCDDFSNYTRCFVIQGSDSLASWQANLFFEPTK 414

Query: 409 FEGTDVLVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFTXXXXXXXXXXXXXXXXX 468
           FE TDVLVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFT                 
Sbjct: 415 FENTDVLVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFTGHSLGGSLSLLVHLMLL 474

Query: 469 ARKXXXXXXXXXXXXFGSPFVFCGGQKLMDELGMDESQIHCVMMHRDIVPRAFSCNYPNH 528
            RK            FGSPFVFCGGQKL+DELG+DE+QIHCVMMHRDIVPRAFSCNYP+H
Sbjct: 475 TRKVVSPSTLKPVVTFGSPFVFCGGQKLIDELGVDENQIHCVMMHRDIVPRAFSCNYPDH 534

Query: 529 VALVLKRLNSTFRSHPCLTKNKLLYSPLGKIFILQPDEKTSPPHPLLPPGSAFYALDSTR 588
           VA++LKRLN TFRSHPCLTKNKLLY+PLGKIFI+QPDE TSPPHPLLP  SAFY LDST 
Sbjct: 535 VAVILKRLNRTFRSHPCLTKNKLLYTPLGKIFIIQPDEMTSPPHPLLPSESAFYELDSTI 594

Query: 589 CGYSPSVLRTFLNQPHPIETLSDPTAYGSEGTILRDHDSSNYLKAVNGILRQHSKILVRR 648
           CGYSP VL +FLNQPHPIETLSDPTAYG+EGTILRDHDSSNYLKAVNGILRQHSK LVRR
Sbjct: 595 CGYSPRVLSSFLNQPHPIETLSDPTAYGAEGTILRDHDSSNYLKAVNGILRQHSKTLVRR 654

Query: 649 VREQRINELWPLLTSPSPHLWSHEQNMERCRFMTKEIITGV 689
           V++QRI+ELWPLLTSPSPH WSHEQNMERC  MT EI TGV
Sbjct: 655 VKKQRIDELWPLLTSPSPHSWSHEQNMERCILMTNEITTGV 695


>Medtr7g109350.1 | triacylglycerol lipase | HC |
           chr7:44694033-44696965 | 20130731
          Length = 656

 Score =  353 bits (906), Expect = 3e-97,   Method: Compositional matrix adjust.
 Identities = 199/491 (40%), Positives = 269/491 (54%), Gaps = 52/491 (10%)

Query: 184 SFSKFLAQVPWTDTKHYSQLAFLCNMAYVIPKIKAM-----DLRRYYSLQFVTSSXXXXX 238
           SFS+ L +V   + + Y+ ++ L N+AY IP IK +     +L +   L+FVTSS     
Sbjct: 141 SFSRMLRRVTLVEARMYAHMSHLGNLAYSIPNIKDLLEIQGNLLKRCGLRFVTSS----- 195

Query: 239 XXXXXXXXXDQDSTRVPIDDSAASQDGSEKCKDNENKHQIRVAYDIAASAASYVQLRAKD 298
                          +   + AAS    E    +  + ++    ++  SA++  ++    
Sbjct: 196 ---------------IEKKELAASIKKEETNGKDAGERKVEKNGELKTSASNACEI---- 236

Query: 299 LLSLAAKSQHSDNNNKDSNGVDSPQQEAEATSRGYKSEVAAYMAAS-TMTSVVAAGERER 357
             ++   S    N + D             T     ++V   MA + +MT+VVAA E  +
Sbjct: 237 --AVVEGSVEGSNGSVD-------------TVNMMDTDVGCLMATTNSMTAVVAANEEVK 281

Query: 358 HETAKDLQSLHSSPCEWFVCDDFSNYTRCFVIQGSDSVASWHANLFFEPTKFEGTDVLVH 417
              A DL S  SSPCEWF+CDD  + TR FVIQGS+S  SW ANL FEP +FEG DVLVH
Sbjct: 282 QSFADDLNSTSSSPCEWFICDDDRSSTRYFVIQGSESFESWQANLLFEPVQFEGLDVLVH 341

Query: 418 RGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFTXXXXXXXXXXXXXXXXXARKXXXXXX 477
           RGIYEAAKG Y+Q +PE+  HLK  G RA  +FT                  RK      
Sbjct: 342 RGIYEAAKGTYQQMLPEVHAHLKSQGSRATFRFTGHSLGGSLALLINLMLLIRKEVPISS 401

Query: 478 XXXXXXFGSPFVFCGGQKLMDELGMDESQIHCVMMHRDIVPRAFSCNYPNHVALVLKRLN 537
                 FGSP + CGG +L+++LG+  S +  + MHRDIVPRAFSC YPNHVA +LK +N
Sbjct: 402 LLPVITFGSPSIMCGGDRLLEKLGLPRSHVQGITMHRDIVPRAFSCTYPNHVAELLKAVN 461

Query: 538 STFRSHPCLTKNKLLYSPLGKIFILQPDEKTSPPHPLLPPGSAFYALDS--TRCGYSPSV 595
           ++FRSHPCL   KLLY+P+G++ ILQPDE  SP H LLPPGS  Y L    +    + + 
Sbjct: 462 ASFRSHPCLNNQKLLYAPMGELMILQPDEFFSPSHHLLPPGSGLYLLSGPLSESNDTENQ 521

Query: 596 LR----TFLNQPHPIETLSDPTAYGSEGTILRDHDSSNYLKAVNGILRQH-SKILVRRVR 650
           L+     FLN PHP+E LSD +AYG  G I RDHD ++YLK+V  ++RQ  S+I     R
Sbjct: 522 LKAAKLVFLNSPHPLEILSDRSAYGPGGAIHRDHDMNSYLKSVRTVIRQELSQIRTAARR 581

Query: 651 EQRINELWPLL 661
           E R    WPL+
Sbjct: 582 ELRRKVRWPLV 592


>Medtr1g087330.1 | triacylglycerol lipase | HC |
           chr1:39148495-39144718 | 20130731
          Length = 560

 Score =  306 bits (783), Expect = 5e-83,   Method: Compositional matrix adjust.
 Identities = 165/393 (41%), Positives = 221/393 (56%), Gaps = 37/393 (9%)

Query: 184 SFSKFLAQVPWTDTKHYSQLAFLCNMAYVIPKIKAMDLRRYYSLQFVTSSXXXXXXXXXX 243
           SFSK L +V   + + Y+Q++ L ++AY IP IK   L ++Y L+FVTSS          
Sbjct: 161 SFSKMLRRVSLAEARLYAQMSHLGSLAYSIPNIKPGKLLKHYGLRFVTSSL--------- 211

Query: 244 XXXXDQDSTRVPIDDSAASQDGSEKCKDNENKHQIRVAYDIAASAA--------SYVQLR 295
                 +   +        Q+   K K  E K      Y I+A+AA        SY+  +
Sbjct: 212 ------EKKELAAKSEKNPQEVESKEKQEETKEPNNGGYRISATAAYNIAASAASYLHAQ 265

Query: 296 AKDLLSLAAKSQHSDNNNKDSNGVDSPQQEAEATSRGYKSEVAAYMAAS-TMTSVVAAGE 354
            K +L   + +  +   + +S  + +P             EV + MA + ++T+VVAA E
Sbjct: 266 TKSILPFTSSNAATGEGSNESLNMMNP-------------EVVSLMATTDSVTAVVAAKE 312

Query: 355 RERHETAKDLQSLHSSPCEWFVCDDFSNYTRCFVIQGSDSVASWHANLFFEPTKFEGTDV 414
             +   A DL S HSSPCEWF+CDD  + TR FVIQGS+S+ASW ANL FEP KFEG DV
Sbjct: 313 EVKQAVADDLNSTHSSPCEWFICDDNQSGTRFFVIQGSESLASWQANLLFEPIKFEGLDV 372

Query: 415 LVHRGIYEAAKGIYEQFMPEIMDHLKRHGDRAKLQFTXXXXXXXXXXXXXXXXXARKXXX 474
           LVHRGIYEAAKGIY Q +PE+  HLK  G RA  +FT                  R+   
Sbjct: 373 LVHRGIYEAAKGIYLQMLPEVHAHLKSRGSRATFRFTGHSLGGSLALLVNLMLFIRQEVP 432

Query: 475 XXXXXXXXXFGSPFVFCGGQKLMDELGMDESQIHCVMMHRDIVPRAFSCNYPNHVALVLK 534
                    FGSP + CGG  L+++LG+  S +  ++MHRDIVPRAFSCNYP+HVA +LK
Sbjct: 433 ISSLLPVITFGSPSIMCGGDTLLEKLGLPRSHVQAIIMHRDIVPRAFSCNYPDHVAKILK 492

Query: 535 RLNSTFRSHPCLTKNKLLYSPLGKIFILQPDEK 567
            +N  FR+HPCL   KLLY+P+G++ ILQPDEK
Sbjct: 493 AINVNFRNHPCLNNQKLLYTPMGELLILQPDEK 525