Miyakogusa Predicted Gene

Lj1g3v4959930.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v4959930.1 Non Characterized Hit- tr|D7U5C7|D7U5C7_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,28,0.000000000004,EF_HAND_2,EF-HAND 2; no description,EF-hand-like
domain; seg,NULL,CUFF.33755.1
         (326 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g115070.1 | calcium-binding EF hand protein, putative | HC...   393   e-109
Medtr4g075610.1 | calcium-binding EF-hand protein | HC | chr4:28...    52   6e-07

>Medtr7g115070.1 | calcium-binding EF hand protein, putative | HC |
           chr7:47509217-47512987 | 20130731
          Length = 333

 Score =  393 bits (1010), Expect = e-109,   Method: Compositional matrix adjust.
 Identities = 219/333 (65%), Positives = 246/333 (73%), Gaps = 9/333 (2%)

Query: 1   MSDGA---LQILDGTHLRNXXXXXXXX-XXXXGAHILDIAHSRVSASLFALPLSDALKAS 56
           MSDG    LQILDGT+LR+             GA +LDIAHSR S SLF LPL D LK S
Sbjct: 1   MSDGGAFQLQILDGTNLRDLDLSSTVFDRAFTGAQLLDIAHSRASLSLFDLPLPDPLKTS 60

Query: 57  ALTRLHLPDADA----FLSAEYDHDKAAEVLRDYIAAIADELKDNPIVASILDGSTISLL 112
           AL RL   ++DA     L  EY   KA+E+L+ YIAAIADELKDNPIV SILDGST+ LL
Sbjct: 61  ALNRLRSSNSDADVVFSLETEYQPAKASEILKLYIAAIADELKDNPIVISILDGSTLRLL 120

Query: 113 LEDEDDFAMLAENLFTDLDVKDKGKISKSEIRNALVQMGAEMGVPPFSEFPLLNDILKKH 172
            EDEDDFAMLAENLFTDLDV+DKGKISKS+IR+ALVQMG +MGVPPFSE+P LND+L+KH
Sbjct: 121 FEDEDDFAMLAENLFTDLDVEDKGKISKSQIRSALVQMGVDMGVPPFSEYPQLNDLLRKH 180

Query: 173 GADGEEELGQAQFAQLLQSVLQDLEPELSKKXXXXXXXXXXXXGSKLRQLLANEPELNSI 232
           GADGEEELGQAQFAQLLQSV+QDLE ELSKK            G K+RQLLANE EL+S 
Sbjct: 181 GADGEEELGQAQFAQLLQSVIQDLEVELSKKNFVFVQNIQIINGFKIRQLLANEKELSSF 240

Query: 233 VERALQEKPEPADGLGSTETIIRGFLEKNAKELGLPLSEADSAVALLYDTVFADVVXXXX 292
           VE+ALQEK    DGL STE IIR FLE++AKELGLPLSEA  A AL YD+VFADV     
Sbjct: 241 VEKALQEKANAKDGLESTE-IIRSFLERDAKELGLPLSEAGDAAALFYDSVFADVAKEKD 299

Query: 293 XXXXXXXXXAKLMKDVLEKFAEQLEFNPVYQDF 325
                    AKL+KD+LEK AEQLE +PVYQ+F
Sbjct: 300 GVELDKEELAKLLKDILEKMAEQLELSPVYQEF 332


>Medtr4g075610.1 | calcium-binding EF-hand protein | HC |
           chr4:28897277-28893295 | 20130731
          Length = 375

 Score = 52.4 bits (124), Expect = 6e-07,   Method: Compositional matrix adjust.
 Identities = 66/278 (23%), Positives = 122/278 (43%), Gaps = 29/278 (10%)

Query: 64  PDADAFLSA---EYDHDKA--------AEVLRDYIAAIADELKDNPIVASILDGSTISLL 112
           PD+D   S    E+ H K          EVL D +  +A  LK +PIV   +DG  +   
Sbjct: 80  PDSDHIYSEVLNEFTHGKQEKVSKTEFKEVLSDILLGMAAGLKRDPIVILRIDGEDLLEF 139

Query: 113 LEDEDDFAMLAENLFTDLDVKDKGKISKSEIRNALVQMGAEMGVPPFSEFPLLNDI---- 168
           L +   +     ++F+ ++   K       I  AL ++  + G+PP S+  ++++I    
Sbjct: 140 L-NGPSYEPEIVSIFSQIESPQKSL--HDLIIQALSKLNVDQGIPPTSDSWVISNIVEPA 196

Query: 169 -LKKHGADGEEELGQAQFAQLLQSVLQDLEPELSKKXXXXXXXXXXXXGSKLRQLLANEP 227
            L +HG D ++ + Q  F +  + V   +   L ++            G+ +++LL+N+ 
Sbjct: 197 LLSEHGHDLDKPVSQEIFLEEFKKVALSVANRLKEQPVIVAHSESTFDGNGVKRLLSNKF 256

Query: 228 ELNSIVERALQEKPEPADGLGSTETIIRGFLEKNAKELGLP----LSEADSAVALLYDTV 283
           EL+ I+  A++  P+  +G  S +  +R  L+  A    LP    + E D  +  ++  V
Sbjct: 257 ELDKILNSAIETLPKDKNGKISKD-YLRVALDTVAPSAALPPFGAIEEMDKVIGEVFKLV 315

Query: 284 FADVVXXXXXXXXXXXXXAKLMKDVLEKFAEQLEFNPV 321
            AD                K++ ++L     QLE NP+
Sbjct: 316 SAD-----DAKLVKEEEFKKILTEILGSIMLQLEGNPI 348