Miyakogusa Predicted Gene

Lj1g3v4515570.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v4515570.1 Non Characterized Hit- tr|I3T4M3|I3T4M3_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,100,0,Cysteine
proteinases,NULL; SUBFAMILY NOT NAMED,NULL; FAMILY NOT NAMED,NULL;
Phytochelatin_C,Phytoche,NODE_37062_length_1633_cov_16.399878.path1.1
         (465 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g097190.1 | phytochelatin synthase | HC | chr7:39092074-39...   763   0.0  
Medtr7g097200.1 | glutathione gamma-glutamylcysteinyltransferase...   512   e-145

>Medtr7g097190.1 | phytochelatin synthase | HC |
           chr7:39092074-39097270 | 20130731
          Length = 501

 Score =  763 bits (1969), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 372/465 (80%), Positives = 389/465 (83%), Gaps = 1/465 (0%)

Query: 1   MESFYRLVSYFQTQSEPAFCGLASLSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPLDK 60
           ME FYRLVSYFQTQSEPAFCGLASLSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPL+ 
Sbjct: 38  MEGFYRLVSYFQTQSEPAFCGLASLSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPLEM 97

Query: 61  IKARGISFGKLVCLAHCAGAKVEAFHASHSSIDHFRKYVMKCSTSDDCHVISSYHREALK 120
           +K+RGISFGKLVCLAHCAGAKV+AFHAS SSI  FRKYV+KCSTSDDCHVISSYHR ALK
Sbjct: 98  VKSRGISFGKLVCLAHCAGAKVDAFHASQSSIHDFRKYVLKCSTSDDCHVISSYHRAALK 157

Query: 121 QTGTGHFSPIGGYHAGKDMALILDVARFKYPPHWIPLTHLWEGMNYVDESTGKTRGFMLI 180
           QTG GHFSPIGGYHAGKDMALILDVARFKYPPHW+PLT LWEGMNY+DESTG++RGFMLI
Sbjct: 158 QTGNGHFSPIGGYHAGKDMALILDVARFKYPPHWVPLTLLWEGMNYIDESTGQSRGFMLI 217

Query: 181 SRPHREPGMLYTLSCKHESWNSIAKFLIDDIPFLLTSEDVKDICKVLSVIVTSLPSNFEE 240
           SRPHREPGMLYTLSCKHESWNSIAKFL+DD+PFLL SEDVKDI KVLSVI TSLPSNFEE
Sbjct: 218 SRPHREPGMLYTLSCKHESWNSIAKFLMDDVPFLLKSEDVKDIHKVLSVIFTSLPSNFEE 277

Query: 241 FIKWVAEIRRGEDGSPSLSVEEKARLSVKEEILKQVQRTGLFKHVASFLSHSCSGHTPTS 300
           FIKWVAEIRR EDG  SLS EEK RL+VKEE+L QVQ T LFKHV+SFLS SC     TS
Sbjct: 278 FIKWVAEIRRREDGDSSLSAEEKTRLAVKEEVLGQVQETRLFKHVSSFLSSSCGRQKLTS 337

Query: 301 GDRDTFPVIAASVCCQGAEILGGKISSSAEYCCRETCMKCWKAEDDKPIRMVCGTVVNGN 360
           GD DT P IAASVCCQGAEIL GK+SSSA YCC ETC KCW AEDDK I MV GTVVNGN
Sbjct: 338 GDGDTLPAIAASVCCQGAEILDGKLSSSAAYCCPETCTKCWNAEDDKSITMVSGTVVNGN 397

Query: 361 TEQGVDVLIPXXXXXXXXXXXXXXXXIRKHPAXXXXXXXXXXXXXXXXWAGIADEKLLSE 420
           TEQGVDVLIP                IR HPA                WAGI D +LL+E
Sbjct: 398 TEQGVDVLIP-SSSGKLCCTCSSKKNIRMHPASTDVLTVLILSLPSTTWAGITDNQLLAE 456

Query: 421 IHDLVSIENLPALLQEEVLHLRRQLHILKRCQEGKVDEDLGVPLS 465
           IHDLVS ENL  LLQEEVLHLRRQLHILKRCQEGKVDEDLG P S
Sbjct: 457 IHDLVSTENLSTLLQEEVLHLRRQLHILKRCQEGKVDEDLGAPSS 501


>Medtr7g097200.1 | glutathione gamma-glutamylcysteinyltransferase |
           HC | chr7:39099161-39103892 | 20130731
          Length = 497

 Score =  512 bits (1319), Expect = e-145,   Method: Compositional matrix adjust.
 Identities = 247/452 (54%), Positives = 317/452 (70%), Gaps = 5/452 (1%)

Query: 1   MESFYRLVSYFQTQSEPAFCGLASLSMVLNALAIDPGRKWKGPWRWFDESMLDCCEPLDK 60
           M  F++L+SY+QTQS+PAFCGLA+LS+VLNALAIDPGRKWKGPWRWFD+SMLDCCEPLDK
Sbjct: 38  MNGFFKLISYYQTQSDPAFCGLATLSVVLNALAIDPGRKWKGPWRWFDDSMLDCCEPLDK 97

Query: 61  IKARGISFGKLVCLAHCAGAKVEAFHASHSSIDHFRKYVMKCSTSDDCHVISSYHREALK 120
           I+++GI+FGK+ CLA C GAKVE F +  S+ID FR  ++ CS+S+DCHVI SY R  L 
Sbjct: 98  IQSQGITFGKVACLARCNGAKVEPFRSDQSNIDDFRNRLISCSSSEDCHVIVSYLRTPLN 157

Query: 121 QTGTGHFSPIGGYHAGKDMALILDVARFKYPPHWIPLTHLWEGMNYVDESTGKTRGFMLI 180
           QTG GHFSP+GGYHA +DMALILDVARFKYPPHW+P+T LW  MN +D+STG+ RG+M+I
Sbjct: 158 QTGIGHFSPLGGYHAERDMALILDVARFKYPPHWVPVTFLWNAMNTIDQSTGQHRGYMII 217

Query: 181 SRPHREPGMLYTLSCKHESWNSIAKFLIDDIPFLLTSEDVKDICKVLSVIVTSLPSNFEE 240
           S+ +R P +LYT+SC+HE W+S+AKFL +D+P LL SED+KDI +VLSV+  S PS   E
Sbjct: 218 SKLNRAPSILYTVSCRHEGWSSVAKFLTEDVPLLLKSEDLKDIQEVLSVVFKSPPSELRE 277

Query: 241 FIKWVAEIRRGEDGSPSLSVEEKARLSVKEEILKQVQRTGLFKHVASFLS--HSCSGHTP 298
           FI WVAE+RR EDG+ +LS EEK RL++K ++L+Q++ T LFKHV  +L    SC     
Sbjct: 278 FITWVAEVRRQEDGNLTLSEEEKGRLAIKADVLEQIRSTALFKHVIRWLDSERSCCDTIA 337

Query: 299 TSGDRDTFPVIAASVCCQGAEILGGKISSSAEYCCRETCMKCWKAEDDKPIRMVCGTVVN 358
             GD D    + A VCCQGA++L G  SSS   CC +  +K      + P+ ++ GTV  
Sbjct: 338 NVGDEDMLSALGARVCCQGADMLTGCRSSSGN-CCSQIDVKHLNVGSENPVTLISGTVTT 396

Query: 359 -GNTEQGVDVLIPX-XXXXXXXXXXXXXXXIRKHPAXXXXXXXXXXXXXXXXWAGIADEK 416
            G++EQGVDVL+P                 I  HP+                W+GI +EK
Sbjct: 397 GGSSEQGVDVLVPLCQRKPSSLCLCNEGQCIGMHPSTADVLTVLLFSLPLHTWSGIKEEK 456

Query: 417 LLSEIHDLVSIENLPALLQEEVLHLRRQLHIL 448
           L  E+  L++ E+L  LL EEVL LR QLH L
Sbjct: 457 LRVEVASLITTEDLLPLLAEEVLFLRDQLHFL 488