Miyakogusa Predicted Gene
- Lj1g3v4371150.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v4371150.1 tr|G7KWA0|G7KWA0_MEDTR Gamma-tubulin complex
component OS=Medicago truncatula GN=MTR_7g093560 PE=4
S,77.98,0,seg,NULL; Spc97_Spc98,Spc97/Spc98; coiled-coil,NULL;
GAMMA-TUBULIN COMPLEX COMPONENT 3 (GCP-3),Gamma,CUFF.32304.1
(777 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr7g093560.1 | tubulin gamma complex-associated protein | HC ... 1236 0.0
Medtr8g011450.1 | Spc97/Spc98 family of spindle pole body (SBP) ... 155 1e-37
Medtr1g088410.1 | tubulin gamma complex-associated protein | HC ... 111 3e-24
Medtr5g004880.2 | Spc97/Spc98 family of spindle pole body (SBP) ... 77 7e-14
Medtr5g004880.1 | Spc97/Spc98 family of spindle pole body (SBP) ... 77 8e-14
Medtr3g101230.1 | Spc97/Spc98 family of spindle pole body (SBP) ... 68 3e-11
>Medtr7g093560.1 | tubulin gamma complex-associated protein | HC |
chr7:37201001-37197287 | 20130731
Length = 841
Score = 1236 bits (3197), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 603/780 (77%), Positives = 673/780 (86%), Gaps = 38/780 (4%)
Query: 1 MDEEEEQQKLPDLVKELVHRLLRHNLPADSPPLNPNSPEFQKPLRYALRILSSRLNPSLS 60
MD+EEEQQKLPDLVK LVH LL NLP +S P+NPNSP+FQ LRYA RILSS L PS++
Sbjct: 1 MDDEEEQQKLPDLVKALVHHLLSQNLPPNSQPINPNSPQFQNSLRYAHRILSSHLTPSIT 60
Query: 61 APDAAAVAEQIKRRLATHGRSADALSFADSPPTPPAAAEQIKRRLAPHGRSADALSFADL 120
PD+AAVA+ I KRRLA GRS++ALSFADL
Sbjct: 61 -PDSAAVADSI------------------------------KRRLATDGRSSEALSFADL 89
Query: 121 YSKFAARATTVNNKWALLYLLEILAEDRKALAQTQFHXXXXXXXXXXXXN----HHHEGP 176
Y+KF+ +AT ++NKW+LL+L I+++DRK+ A++ N + G
Sbjct: 90 YTKFSTKATHIDNKWSLLHLFNIISQDRKSAAKSNDPSVLLPNLTISENNVQPRGENNGF 149
Query: 177 NDGVLLLAKDPENRRDIAFREFVNLVREENEVSEEALVKDVLYACQGVEGKYVKFDVVNN 236
NDGV++LA+DP+NRR+IAF E+V L++EEN+V+EEA+V DV+YACQGV+GKYVKFD ++
Sbjct: 150 NDGVVILARDPKNRREIAFNEYVKLIKEENDVTEEAMVTDVIYACQGVDGKYVKFDEESD 209
Query: 237 CYALLDSIRVTRATRSMVHKLSELGGLFKKVTGYIWQSMDRFPAEDVGTVGQAFCSALQD 296
Y LLDSIRV+RA+ SMV KL ELG LFK+V G+I S+ RFPAEDVGTVGQAFC+ALQD
Sbjct: 210 GYVLLDSIRVSRASSSMVFKLCELGVLFKRVVGFIEMSLGRFPAEDVGTVGQAFCAALQD 269
Query: 297 ELSEYYKLLAVLEAQSSNPIPLVSESASSGNYLSLRRLAVWLAEPMVKMRLMSDLVDKCK 356
ELS+YYKLLAVLEAQSSNPIPL+SE SSGNYLSLRRLAVWLAEPMVKM+LM+DLV+KC+
Sbjct: 270 ELSDYYKLLAVLEAQSSNPIPLLSEGVSSGNYLSLRRLAVWLAEPMVKMKLMADLVEKCR 329
Query: 357 ILRGGAMAGAIHLHAQHGDPLVHEFMKRLLQRVCSPLFEMVRRWVLEGELEDIFAEFFIV 416
+LRGGAMAGAIHLHA+HGDP+VHEFMKRLL+RVCSPLFEMV+RWVLEGELEDIF EFFIV
Sbjct: 330 VLRGGAMAGAIHLHARHGDPMVHEFMKRLLRRVCSPLFEMVKRWVLEGELEDIFVEFFIV 389
Query: 417 GQPVKAESLWREGYRLHEVMLPSFIPSSLAQRILRTGKSINFLRVCCEDRGWADAANEVA 476
GQPVKAESLWREGYRL++ MLPSFI +SLAQRILRTGKSINFLRVCCEDRGWA AA E
Sbjct: 390 GQPVKAESLWREGYRLYDAMLPSFISASLAQRILRTGKSINFLRVCCEDRGWARAATE-- 447
Query: 477 TDTGATARRGGFGYGETDTLESLVDEAAKRIDKHLLDVIYTRYKFKDHCLAIKRYLLLGQ 536
DTGA ARRGGFGYGETDTLESLVDEA+KRIDKHLLDVIY RYKFK+HCLAIKRYLLLGQ
Sbjct: 448 -DTGAMARRGGFGYGETDTLESLVDEASKRIDKHLLDVIYERYKFKEHCLAIKRYLLLGQ 506
Query: 537 GDFVQYLMDIVGPELSEPANTISSFKLAGLLETAIRASNAQYDDPDILDRLRVKMMPHES 596
GDFVQYLMDIVGPELS PANTISSFKLAGLLETAIRASNAQYDDPDILDRLRVKMMPHES
Sbjct: 507 GDFVQYLMDIVGPELSVPANTISSFKLAGLLETAIRASNAQYDDPDILDRLRVKMMPHES 566
Query: 597 GDRGWDVFSLEYDASVPLDTVFTESVMAKYLRIFNFLWKLKRVEHALIGAWKTMKPNGIT 656
GDRGWDVFSLEYDA VPLDTVFTESVMA+YLRIFNFLWKLKRVEHALIGAWKTMKPN IT
Sbjct: 567 GDRGWDVFSLEYDARVPLDTVFTESVMARYLRIFNFLWKLKRVEHALIGAWKTMKPNCIT 626
Query: 657 SSSYTRLQHAVKMQLVSTLRRCQVLWVEINHFISNLQYYIMFEVLEVSWSDLLSEMEVAK 716
S+++ RLQHAVKMQLVS LRRCQVLWVEINHFISNLQYYIMFEVLE+SWS+ LSEMEVAK
Sbjct: 627 SNTFNRLQHAVKMQLVSALRRCQVLWVEINHFISNLQYYIMFEVLEISWSNFLSEMEVAK 686
Query: 717 DLDDLLAAHEKYLHSIVEKSLLGELSQSLYKSLLVIFDLILRFRSHADRLYEGIHELQAR 776
DLDDLLAAHEKY++SIVEKSLLGELSQSLYKSL+VIFDLILRFRSHAD LYEGIHELQAR
Sbjct: 687 DLDDLLAAHEKYMNSIVEKSLLGELSQSLYKSLIVIFDLILRFRSHADILYEGIHELQAR 746
>Medtr8g011450.1 | Spc97/Spc98 family of spindle pole body (SBP)
component | HC | chr8:3076171-3085297 | 20130731
Length = 664
Score = 155 bits (393), Expect = 1e-37, Method: Compositional matrix adjust.
Identities = 150/569 (26%), Positives = 256/569 (44%), Gaps = 93/569 (16%)
Query: 208 VSEEALVKDVLYACQGVEGKYVKFDVV----NNCYALLDSIRVTRATRSMVHKLSELGGL 263
V E ++ +VL A GVEG+Y+ + + L + A + + ++ L
Sbjct: 20 VQELIVIDEVLSAMVGVEGRYISIKTLRGKKDEIINFLVDPSMDLALQELAKRIFPLCRS 79
Query: 264 FKKVTGYIWQSMDRFPAEDVGTVGQAFCSALQDELSEYYKLLAVLEAQSSNPIPLVSESA 323
F + ++ +S +F + G V AF +AL+ L +Y ++A LE Q
Sbjct: 80 FLLINQFV-ESRSQF---ENGLVNHAFSAALRAFLLDYQAMVAQLEHQ------------ 123
Query: 324 SSGNYLSLRRLAV---WL-AEPMVK-MRLMSDLVDKCKI--LRGGAMAGAIHLHAQH--G 374
L RL++ W +PM++ M+ +S ++ K + G A+ + A+ G
Sbjct: 124 -----FRLGRLSLQGLWFYCQPMLRSMQALSTVIQKASVNNFTGSAVLNLLQSQAKAMAG 178
Query: 375 DPLVHEFMKRLLQRVCSPLFEMVRRWVLEGELEDIFAEFFIV-GQPVKAESL-------- 425
D V ++++ Q ++ RWV EG ++D + EFFI + ++ ESL
Sbjct: 179 DNAVRLLLEKMTQCASRAYMSILERWVYEGVIDDPYGEFFIAEDKSLQKESLTKDYDAKY 238
Query: 426 WREGYRLHEVMLPSFIPSSLAQRILRTGKSINFLRVCCEDRGWADAANEVATDTGATARR 485
WR+ Y L + +PSF+ +S A IL TGK +N LR C + V ++
Sbjct: 239 WRQRYSLKD-GIPSFLANS-AGTILTTGKYLNVLREC---------GHNVQVPPSENSKL 287
Query: 486 GGFGYGETDTLESLVDEAAKRIDKHLLDVIYTRYKFKDHCLAIKRYLLLGQGDFVQYLMD 545
FG LE + A L+++I +Y +IK YLLL QGDF+ + MD
Sbjct: 288 MSFG-SNHHYLEC-IKAAYNFASGELVNLIKEKYDLTGKLRSIKHYLLLDQGDFLVHFMD 345
Query: 546 IVGPELSEPANTISSFKLAGLLETAIRASNAQYDD-----------PDILDRLRV----- 589
I EL++ + +S KL LL+ A+R + A D +L RL
Sbjct: 346 IARDELAKKPDEVSVEKLQSLLDLALRTTAAAVDPYHEGLTCIVERSSLLKRLGTFNVTE 405
Query: 590 ---------KMMPHESGDRGWDVFSLEYDASVPLDTVFTESVMAKYLRIFNFLWKLKRVE 640
++ G + FSL Y A PL V + + KY IF FL+ K V+
Sbjct: 406 VNQRDVINNDILEEPVSITGLETFSLSYKAHWPLSIVLSRKALTKYQLIFRFLFHCKHVD 465
Query: 641 HALIGAWKTMKPNGITSSSYTRLQHAVKMQLVSTLRRCQVLWVEINHFISNLQYYIMFEV 700
L GAW+T + G+ + + TR + + R +L + F+++L +Y+ FEV
Sbjct: 466 RQLCGAWQTHQ--GVRALN-TR---------GTAISRSSLLCRSMLKFVNSLLHYLTFEV 513
Query: 701 LEVSWSDLLSEMEVAKDLDDLLAAHEKYL 729
+E +W + + ++ A +D+++ H+ +L
Sbjct: 514 IEPNWHMMYTRLQSANSIDEVIQHHDFFL 542
>Medtr1g088410.1 | tubulin gamma complex-associated protein | HC |
chr1:39524666-39532429 | 20130731
Length = 739
Score = 111 bits (277), Expect = 3e-24, Method: Compositional matrix adjust.
Identities = 109/478 (22%), Positives = 183/478 (38%), Gaps = 98/478 (20%)
Query: 358 LRGGAMAGAIHLHAQHGDPLVHEFMKRLLQRVCSPLFEMVRRWVLEGELEDIFAEFFIVG 417
+RGG + +H G P + ++RLL ++ + W++ G LED EFFI
Sbjct: 160 IRGGELLNLLHKKCHCGVPELQTCIQRLLWHGHQVMYNQLASWMVYGILEDRHGEFFISR 219
Query: 418 QPVK-----------AESL------------WREGYRLHEVMLPSFIPSSLAQRILRTGK 454
Q + +E L W G+ + MLP +IP +A+ IL GK
Sbjct: 220 QEGRDVENSSSHQEISEKLSRLSTADASLSDWHMGFHISLDMLPEYIPMRVAESILFAGK 279
Query: 455 SINFLR-----VCCED----------------RGWADAANEVATDTGATARRGGFGYGET 493
++ LR +D G + E +TG E
Sbjct: 280 AVRVLRNPSPSFLSQDDVYPQEPKRFPKIHGFEGRFNFQREPIINTGMRVE-DLLPQSEA 338
Query: 494 DTLESL------------------VDEAAKRIDKHLLDVIYTRYKFKDHCLAIKRYLLLG 535
D +E++ VD HL ++ R H A+K Y LL
Sbjct: 339 DKIENMLLDLKESSEFHKRSFECAVDSIQAIAASHLWQLVVVRADLNGHLKALKDYFLLA 398
Query: 536 QGDFVQ-------YLMDIVGPELSEPANTISSFKLAGLLETA------------------ 570
+GDF Q LM + + + A+ + F+LA L
Sbjct: 399 KGDFFQCFLEESRQLMRLPPRQSTAEADLMVPFQLASLKTIGEEDKYFSKVSLRMPSYGI 458
Query: 571 ------IRASNAQYDDPDILDRLRVKMMPHESGDRGWDVFSLEYDASVPLDTVFTESVMA 624
+ A D + + E GWD +LEY PL FT+ V++
Sbjct: 459 TVKPSLLNVPKATSAAADGISGASISNASSEMSVDGWDGIALEYSIEWPLHLFFTQEVLS 518
Query: 625 KYLRIFNFLWKLKRVEHALIGAWKTMKPNGITSSSYTRLQHAVKMQLVSTL-RRCQVLWV 683
+YL++F +L +LKR + L W ++ + + + K + +R + +W
Sbjct: 519 RYLKVFQYLLRLKRTQMELEKLWASVMHQYHSIFAKNKKSDQDKSPITQQRDQRFRSMWR 578
Query: 684 EINH---FISNLQYYIMFEVLEVSWSDLLSEMEVAKDLDDLLAAHEKYLHSIVEKSLL 738
H I NLQ+YI +V+E W+ L S ++ + D +L+ H++YL +++ ++ L
Sbjct: 579 VREHMAFLIRNLQFYIQVDVIESQWNILQSHIQDSHDFTELVGFHQEYLSALISQTFL 636
>Medtr5g004880.2 | Spc97/Spc98 family of spindle pole body (SBP)
component | HC | chr5:175920-164883 | 20130731
Length = 1187
Score = 77.0 bits (188), Expect = 7e-14, Method: Compositional matrix adjust.
Identities = 67/268 (25%), Positives = 125/268 (46%), Gaps = 21/268 (7%)
Query: 507 IDKHLLDVIYTRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTISSFKLAGL 566
+ K +++V+ +K ++H LA++RY + D+ + + S T ++ GL
Sbjct: 851 VSKLIINVLEEAFKLQEHLLALRRYHFMELADWADLFILSLWRHWSVTEATERLPEIQGL 910
Query: 567 LETAIRASNAQYDDPDILDRLRVKM-----MPHES---GDRGWDVFSLEYDASVPLDTVF 618
LE +I+ S+ + D +RL V M +P + G R +D L Y PL +
Sbjct: 911 LELSIQKSSCEQDTNK--NRLFVYMKGRGKLPLSASAIGLRSFDFLGLGYHVDWPLCIIL 968
Query: 619 TESVMAKYLRIFNFLWKLKRVEHALIGAWKTMKPNGITSSSYTRLQ----HAVKMQLVST 674
T + + Y IF+FL ++K +L W ++K T++ + A + ++
Sbjct: 969 TPAALKIYADIFSFLIQVKLALFSLTDVWCSLKDMAHTTNKGLNAEPYQPGAGHLNILMK 1028
Query: 675 LRRCQVLWVEINHFISNLQYYIMFEVLEVSWSDLLSEME-VAKDLDDLLAAHEKYLHSIV 733
+R +I+HF+S LQ Y+ ++ VSW L ++ KD+ DL + H +YL +
Sbjct: 1029 MRH------QISHFVSTLQQYVESQLSHVSWCRFLHSLQHKVKDMMDLESVHTEYLADSL 1082
Query: 734 EKSLLGELSQSLYKSLLVIFDLILRFRS 761
L + ++++ + I L FRS
Sbjct: 1083 SICFLSDETKAVGSIIESILQCALDFRS 1110
>Medtr5g004880.1 | Spc97/Spc98 family of spindle pole body (SBP)
component | HC | chr5:175920-164883 | 20130731
Length = 1188
Score = 76.6 bits (187), Expect = 8e-14, Method: Compositional matrix adjust.
Identities = 67/269 (24%), Positives = 128/269 (47%), Gaps = 22/269 (8%)
Query: 507 IDKHLLDVIYTRYKFKDHCLAIKRYLLLGQGDFVQ-YLMDIVGPELSEPANTISSFKLAG 565
+ K +++V+ +K ++H LA++RY + D+ +++ + + S T ++ G
Sbjct: 851 VSKLIINVLEEAFKLQEHLLALRRYHFMELADWADLFILSLWRHKWSVTEATERLPEIQG 910
Query: 566 LLETAIRASNAQYDDPDILDRLRVKM-----MPHES---GDRGWDVFSLEYDASVPLDTV 617
LLE +I+ S+ + D +RL V M +P + G R +D L Y PL +
Sbjct: 911 LLELSIQKSSCEQDTNK--NRLFVYMKGRGKLPLSASAIGLRSFDFLGLGYHVDWPLCII 968
Query: 618 FTESVMAKYLRIFNFLWKLKRVEHALIGAWKTMKPNGITSSSYTRLQ----HAVKMQLVS 673
T + + Y IF+FL ++K +L W ++K T++ + A + ++
Sbjct: 969 LTPAALKIYADIFSFLIQVKLALFSLTDVWCSLKDMAHTTNKGLNAEPYQPGAGHLNILM 1028
Query: 674 TLRRCQVLWVEINHFISNLQYYIMFEVLEVSWSDLLSEME-VAKDLDDLLAAHEKYLHSI 732
+R +I+HF+S LQ Y+ ++ VSW L ++ KD+ DL + H +YL
Sbjct: 1029 KMRH------QISHFVSTLQQYVESQLSHVSWCRFLHSLQHKVKDMMDLESVHTEYLADS 1082
Query: 733 VEKSLLGELSQSLYKSLLVIFDLILRFRS 761
+ L + ++++ + I L FRS
Sbjct: 1083 LSICFLSDETKAVGSIIESILQCALDFRS 1111
>Medtr3g101230.1 | Spc97/Spc98 family of spindle pole body (SBP)
component | HC | chr3:46567256-46577932 | 20130731
Length = 1001
Score = 68.2 bits (165), Expect = 3e-11, Method: Compositional matrix adjust.
Identities = 68/307 (22%), Positives = 123/307 (40%), Gaps = 40/307 (13%)
Query: 496 LESLVDEAAKRIDKHLLDVIYTRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPA 555
L + + + I ++L + ++ + ++ LLG GD +Q+ ++ +L +
Sbjct: 636 LTAYIQKQVDYIGVNMLLKLMNEWRLMEELAVLRAIYLLGSGDLLQHFSTVIFDKLDKGE 695
Query: 556 NTISSFKLAGLLETAIR--ASNAQYDDPDILDRLRVKMMPHESGD--------------- 598
F+L +L+ +IR A PD L K + G+
Sbjct: 696 TWDDDFELNTILQESIRNSADCMLLSAPDSLVVSITKNIVESDGEASTSGSVLGTHKSRV 755
Query: 599 -----RGWDVFSLEYDASVPLDTVFTESVMAKYLRIFNFLWKLKRVEHALIGAWKTMKPN 653
G D+ Y PL+ + + KY ++ FL K+KR + L + M
Sbjct: 756 NNLGMNGLDMLKFTYKVPWPLELIANTEAIKKYNQVMRFLLKVKRAKFVLDKVRRWMW-K 814
Query: 654 GITSSSYTRLQHAVKMQLVSTLRRCQVLWVEINHFISNLQYYIMFEVLEVSWSDLLSEME 713
G S++ R H + Q ++ HF+ Y+M V +W +L M
Sbjct: 815 GRGSTTNNRKHHWLVEQ-------------KLLHFVDAFHQYVMDRVYHSAWRELCESMT 861
Query: 714 VAKDLDDLLAAHEKYLHSIVEKSLL--GELSQSLYKSLLVIFDLILRFRSHADRLYEG-- 769
VAK LD+++ AHE Y+ SI + + +L + + +I L L F + L G
Sbjct: 862 VAKSLDEVIEAHEAYMLSIQRQCFVVPDKLGALIASRVNIILGLALDFYTIQQTLKSGGA 921
Query: 770 IHELQAR 776
+ ++AR
Sbjct: 922 VSAIKAR 928