Miyakogusa Predicted Gene

Lj1g3v3964650.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v3964650.1 Non Characterized Hit- tr|I3T8T8|I3T8T8_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,99.21,0,HNH,HNH
endonuclease; HNH nucleases,HNH nuclease,CUFF.31585.1
         (317 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g085420.1 | HNH endonuclease domain protein | HC | chr7:33...   386   e-107
Medtr7g085420.2 | HNH endonuclease domain protein | HC | chr7:33...   275   5e-74

>Medtr7g085420.1 | HNH endonuclease domain protein | HC |
           chr7:33088436-33083107 | 20130731
          Length = 273

 Score =  386 bits (992), Expect = e-107,   Method: Compositional matrix adjust.
 Identities = 212/294 (72%), Positives = 236/294 (80%), Gaps = 24/294 (8%)

Query: 27  MAQFTTHGRLKLLFNGEVGSSILEHNKDPFHYKCSRSALTRKRRPTRYMGSSA-GGILRY 85
           MAQFT  GRLKLLFNGE  SS++                T KRR T Y GSS+      Y
Sbjct: 1   MAQFTAQGRLKLLFNGETVSSLMVQ--------------THKRR-TSYKGSSSFASRFHY 45

Query: 86  ATSS-MRNKANRCNAEARFTAGNEGVHDEYEYDSDGVEDFDYEDDVFGFDGDGLSCFRGL 144
           A+SS +R+K   CNAEAR    +EGVHDEY Y  +  ED D +D     +GDGLS FRGL
Sbjct: 46  ASSSYLRSKVKCCNAEAR---PSEGVHDEYAYVFEYDEDDDDDDVF---EGDGLSGFRGL 99

Query: 145 VLDLAYRPVNVVCWKRAICLEFMEKADVLEYYEKTVNSPSGSFYIPAVLRVPHLLQVVKR 204
           VLD++YRPVNVVCWKRAICLEFMEKADVLEYY+KTVNSPSGSFYIPAVLRV HLLQV+KR
Sbjct: 100 VLDISYRPVNVVCWKRAICLEFMEKADVLEYYDKTVNSPSGSFYIPAVLRVRHLLQVIKR 159

Query: 205 R-IVKNNLSRKNILFRDHFTCQYCSSRENLTIDHVVPAALGGEWTWENLVTACAKCNSKK 263
           R +VKN+LSRKNILFRD++TCQYCSS ENLTIDHVVPAALGGEWTWENLVTAC+KCNSKK
Sbjct: 160 RRVVKNSLSRKNILFRDNYTCQYCSSHENLTIDHVVPAALGGEWTWENLVTACSKCNSKK 219

Query: 264 GKKSLEEAKMKLNKVPKAPKEYDILAIPLTAAALRMLSKRKGTPEEWRQYLRSS 317
           G+KSLEEAKMKL KVPK PK+YDILAIPLTAAALR+L+ RKGTP+EWRQYL SS
Sbjct: 220 GRKSLEEAKMKLMKVPKKPKDYDILAIPLTAAALRVLTARKGTPDEWRQYLSSS 273


>Medtr7g085420.2 | HNH endonuclease domain protein | HC |
           chr7:33088480-33084380 | 20130731
          Length = 214

 Score =  275 bits (702), Expect = 5e-74,   Method: Compositional matrix adjust.
 Identities = 158/230 (68%), Positives = 176/230 (76%), Gaps = 24/230 (10%)

Query: 27  MAQFTTHGRLKLLFNGEVGSSILEHNKDPFHYKCSRSALTRKRRPTRYMGSSA-GGILRY 85
           MAQFT  GRLKLLFNGE  SS++                T KRR T Y GSS+      Y
Sbjct: 1   MAQFTAQGRLKLLFNGETVSSLMVQ--------------THKRR-TSYKGSSSFASRFHY 45

Query: 86  ATSS-MRNKANRCNAEARFTAGNEGVHDEYEYDSDGVEDFDYEDDVFGFDGDGLSCFRGL 144
           A+SS +R+K   CNAEAR    +EGVHDEY Y  +  ED D +D     +GDGLS FRGL
Sbjct: 46  ASSSYLRSKVKCCNAEAR---PSEGVHDEYAYVFEYDEDDDDDDVF---EGDGLSGFRGL 99

Query: 145 VLDLAYRPVNVVCWKRAICLEFMEKADVLEYYEKTVNSPSGSFYIPAVLRVPHLLQVVK- 203
           VLD++YRPVNVVCWKRAICLEFMEKADVLEYY+KTVNSPSGSFYIPAVLRV HLLQV+K 
Sbjct: 100 VLDISYRPVNVVCWKRAICLEFMEKADVLEYYDKTVNSPSGSFYIPAVLRVRHLLQVIKR 159

Query: 204 RRIVKNNLSRKNILFRDHFTCQYCSSRENLTIDHVVPAALGGEWTWENLV 253
           RR+VKN+LSRKNILFRD++TCQYCSS ENLTIDHVVPAALGGEWTWENLV
Sbjct: 160 RRVVKNSLSRKNILFRDNYTCQYCSSHENLTIDHVVPAALGGEWTWENLV 209