Miyakogusa Predicted Gene

Lj1g3v3690180.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v3690180.1 tr|D0VY45|D0VY45_LOTJA Homocitrate synthase
OS=Lotus japonicus GN=Fen1 PE=2
SV=1,87.91,0,AIPM_HOMOCIT_SYNTH_1,Alpha-isopropylmalate/homocitrate
synthase, conserved site; AIPM_HOMOCIT_SYNTH_,CUFF.31214.1
         (547 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g116500.1 | 2-isopropylmalate synthase | HC | chr1:5269266...   773   0.0  
Medtr7g033125.1 | 2-isopropylmalate synthase | HC | chr7:1170615...   690   0.0  

>Medtr1g116500.1 | 2-isopropylmalate synthase | HC |
           chr1:52692665-52700783 | 20130731
          Length = 598

 Score =  773 bits (1995), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 382/542 (70%), Positives = 432/542 (79%), Gaps = 12/542 (2%)

Query: 15  RNRPEYIPGRIPDSTYVRILDTTLRDGEQAPGAAMTRDQKLEVARQLVKLGVDIIEAGFP 74
           R+RP YIP  IPD +YVRI DTTLRDGEQ+PGA+MT  +KL++ARQL KLGVDIIEAGFP
Sbjct: 57  RHRPPYIPNHIPDPSYVRIFDTTLRDGEQSPGASMTSKEKLDIARQLAKLGVDIIEAGFP 116

Query: 75  SASKEDFMAVKMIAEEVGNLTDDDGYVPVIGGVCRCNEKDIATTWEALKHAKRPRILTFI 134
           +AS +DF AVKMIA EVGN  DD+GYVPVI G+ RCNE DI T W A+KHAKRPRI TFI
Sbjct: 117 AASTDDFEAVKMIANEVGNAVDDNGYVPVICGLSRCNENDIQTAWNAVKHAKRPRIHTFI 176

Query: 135 ATSPIHMELKLRKSKDQVLEIARDMVKYARSLGCTDIGFAAEDAARSDREFLYQILGEVI 194
           ATS IHME KLR SK QV++ AR MV +ARSLGC D+ F+ EDA RSDREFLYQILGEVI
Sbjct: 177 ATSAIHMEFKLRMSKQQVIDKARTMVAFARSLGCQDVEFSPEDAGRSDREFLYQILGEVI 236

Query: 195 KAGATTLTIPDTVGIAMPFEYGKLIADIKENTPGIENAIISTHCHNDLGLATSNTIEGAR 254
           KAGATTL IPDTVGI MP E+GKLIADIK NTPG+EN IISTHC NDLGL+T+NTIEGAR
Sbjct: 237 KAGATTLNIPDTVGITMPSEFGKLIADIKANTPGVENVIISTHCQNDLGLSTANTIEGAR 296

Query: 255 YGARQLEVTINGIGERAGNASLEEVVMALTCRGNDILGGLHTRINTRHILKTSKMVEKYS 314
            GARQLEVTINGIGERAGNASLEEVVMAL C G  I   L T INTRHI  TS+MVE+YS
Sbjct: 297 AGARQLEVTINGIGERAGNASLEEVVMALKC-GGHIFNNLFTGINTRHIYLTSRMVEEYS 355

Query: 315 GLHLQPHKALVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLDRSVGDTIVLGKLSGR 374
           GL LQPHKALVGANAF HESGIHQDGMLKH+GTYEIISPEDIGL+RS    IVLGKLSGR
Sbjct: 356 GLQLQPHKALVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNEAGIVLGKLSGR 415

Query: 375 QALRNRLEELGYKLKDTEVESVFWQFKAVAETKKRITDADLRALVSNEALDAQPIWRLGD 434
            A+R RLEELGY+LKD +VES+FW+FKAVAE KKRITDADLRALVS+E   A+PIW+L D
Sbjct: 416 HAMRKRLEELGYELKDDQVESLFWRFKAVAEQKKRITDADLRALVSDEVFQAEPIWKLDD 475

Query: 435 LQVTCGTVGFSTATVKLFSIDGSMHFACSIGTGPVDSAYKAINHIVKEPVKLVKYTLGAI 494
           LQVTCGT+G STATVKL   DGS H ACS+GTGPVDSAYKA++ IVKEP  L++Y++ A+
Sbjct: 476 LQVTCGTLGLSTATVKLVGDDGSTHIACSVGTGPVDSAYKAVDLIVKEPATLLEYSMIAV 535

Query: 495 TGCMDATATTSVEI---SHEDMNHPI--------FXXXXXXXXXXXXXXEAYLSALNNML 543
           T  +DA ATT V I   + +   H +        F              +AY++A+N ML
Sbjct: 536 TEGIDAIATTRVVIRGDTDQTSTHALTGESVQRTFSGTGAGMDVVVSSVKAYIAAINKML 595

Query: 544 RF 545
            F
Sbjct: 596 SF 597


>Medtr7g033125.1 | 2-isopropylmalate synthase | HC |
           chr7:11706156-11719267 | 20130731
          Length = 436

 Score =  690 bits (1781), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 329/416 (79%), Positives = 373/416 (89%)

Query: 20  YIPGRIPDSTYVRILDTTLRDGEQAPGAAMTRDQKLEVARQLVKLGVDIIEAGFPSASKE 79
           Y P +IP  TYVRILDTTLRDGEQ+PGAAM+  +KL++A  L KL VD+IEAGFPSAS +
Sbjct: 21  YTPNQIPIPTYVRILDTTLRDGEQSPGAAMSCKEKLDIAHLLAKLRVDVIEAGFPSASND 80

Query: 80  DFMAVKMIAEEVGNLTDDDGYVPVIGGVCRCNEKDIATTWEALKHAKRPRILTFIATSPI 139
           DFMAVKMIA+EVGN  D+DG+VPVIGG+CRCNEKDIA TWEA+KHAKRPRI TF+ATSPI
Sbjct: 81  DFMAVKMIAQEVGNDVDNDGHVPVIGGMCRCNEKDIAITWEAVKHAKRPRICTFLATSPI 140

Query: 140 HMELKLRKSKDQVLEIARDMVKYARSLGCTDIGFAAEDAARSDREFLYQILGEVIKAGAT 199
           HME KLRK+KDQVL+IAR+ VK+ARSLGC DI F  EDA RS++EFLYQ+LGEVIKAGAT
Sbjct: 141 HMEYKLRKTKDQVLKIARETVKFARSLGCCDIQFGVEDATRSEKEFLYQVLGEVIKAGAT 200

Query: 200 TLTIPDTVGIAMPFEYGKLIADIKENTPGIENAIISTHCHNDLGLATSNTIEGARYGARQ 259
           T+ IPDTVGIAMPFEYG LIADIK NTPGIEN II+THCHNDLGLAT+NTIEGAR GARQ
Sbjct: 201 TVVIPDTVGIAMPFEYGNLIADIKMNTPGIENVIIATHCHNDLGLATANTIEGARAGARQ 260

Query: 260 LEVTINGIGERAGNASLEEVVMALTCRGNDILGGLHTRINTRHILKTSKMVEKYSGLHLQ 319
           LEVTINGIGERAGNASLEEVVMALTCRGND+LGGLHT INTRHI++TSKMVE+YSG+ LQ
Sbjct: 261 LEVTINGIGERAGNASLEEVVMALTCRGNDVLGGLHTGINTRHIIRTSKMVEEYSGMLLQ 320

Query: 320 PHKALVGANAFLHESGIHQDGMLKHRGTYEIISPEDIGLDRSVGDTIVLGKLSGRQALRN 379
           PHKALVGANAFLHESGIHQDGMLKH+GTYEIISPEDIGL+RS+  +IVLGKLSGRQALR 
Sbjct: 321 PHKALVGANAFLHESGIHQDGMLKHKGTYEIISPEDIGLERSIEASIVLGKLSGRQALRK 380

Query: 380 RLEELGYKLKDTEVESVFWQFKAVAETKKRITDADLRALVSNEALDAQPIWRLGDL 435
           RLEE GYKLKD E+E++FW+FKA+A TKKR+T+ADL+ L+SNEA   + +  L DL
Sbjct: 381 RLEEHGYKLKDDELETLFWKFKALAGTKKRVTNADLKILMSNEAYQDELVHNLHDL 436