Miyakogusa Predicted Gene

Lj1g3v2840270.2
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v2840270.2 Non Characterized Hit- tr|I1L681|I1L681_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.56586
PE,83.87,0,seg,NULL; no description,Peptide chain release factor;
prfB: peptide chain release factor 2,Peptide ,CUFF.29595.2
         (461 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g080430.1 | peptide chain release factor 2 | HC | chr7:305...   730   0.0  
Medtr7g112100.1 | peptide chain release factor 2 | HC | chr7:460...   254   9e-68
Medtr1g041890.1 | peptide chain release factor, putative | HC | ...   253   3e-67
Medtr7g110570.1 | peptide chain release factor 1 | HC | chr7:453...   149   6e-36
Medtr7g110570.2 | peptide chain release factor 1 | HC | chr7:453...   145   6e-35
Medtr7g108300.1 | peptide chain release factor 1 | HC | chr7:440...   145   1e-34
Medtr6g059330.1 | class I peptide chain release factor | HC | ch...    53   6e-07

>Medtr7g080430.1 | peptide chain release factor 2 | HC |
           chr7:30593686-30589146 | 20130731
          Length = 475

 Score =  730 bits (1884), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 377/474 (79%), Positives = 403/474 (85%), Gaps = 17/474 (3%)

Query: 3   VVTQPLCTTTTAAIVTAKSFRR-KQPLLIPQNPTYPSK------HFPF---RTTLXXXXX 52
           VV+ PLC   T   VTAK+FR  KQP LIPQ P+  S       HFPF   RTT      
Sbjct: 4   VVSHPLCCYYTTT-VTAKTFRSYKQPFLIPQFPSSSSSSSSSSPHFPFLSFRTTTLSHRP 62

Query: 53  X---XXXXXXXXXXENQLS-VGEGE-DTDTTEWALQAFYSLRKDVEITSQRVEEIRESSG 107
                         ENQLS VGEGE DT T++ ALQ FYSLRKDVEI SQRV+EIRESSG
Sbjct: 63  SPFPLSPVVLFATPENQLSSVGEGEEDTHTSDSALQDFYSLRKDVEIASQRVKEIRESSG 122

Query: 108 IXXXXXXXXXXXXKASATSFWDNRAKAQETLSTLSDVKDKINLLAEFKTQVEDAETIVKL 167
           +            +AS +SFWD+RAKAQ+TLSTL+DVK+KI LL ++KTQVEDAETIV L
Sbjct: 123 LQLLEQELAKLEEEASCSSFWDDRAKAQQTLSTLADVKEKIKLLNDYKTQVEDAETIVML 182

Query: 168 TEEMESSIDRALLEEATSLIKELNKSLDRFELTQLLSGPYDKEGAVISITAGAGGTDAQD 227
           TEEMES +D+ L EEA+SLIKELNKS+DRFELTQLLSGPYDKEGAVISITAGAGGTDAQD
Sbjct: 183 TEEMES-VDKGLYEEASSLIKELNKSIDRFELTQLLSGPYDKEGAVISITAGAGGTDAQD 241

Query: 228 WADMLLRMYMRWGEKQRYKTRVVERSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVR 287
           WADMLLRMY+RWGEKQRYKTRVVE+S GEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVR
Sbjct: 242 WADMLLRMYVRWGEKQRYKTRVVEKSLGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVR 301

Query: 288 QSPFNSKGLRQTSFSGVEVMPLLPEESLDVEIPEEDLEISFSRAGGKGGQNVNKVETAVR 347
           QSPFNSKGLRQTSFSGVEVMPLLPEESL+VEIPEEDLEISFSRAGGKGGQNVNKVETAVR
Sbjct: 302 QSPFNSKGLRQTSFSGVEVMPLLPEESLNVEIPEEDLEISFSRAGGKGGQNVNKVETAVR 361

Query: 348 ITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQ 407
           ITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRA+E KQIRGD VKAEWGQQ
Sbjct: 362 ITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRATEFKQIRGDVVKAEWGQQ 421

Query: 408 IRNYVFHPYKLVKDVRTGHETPDVTSVMDGELDPFIKSYLKHKFSMTMSTSGVN 461
           IRNYVFHPYKLVKDVRTGHETPD+TSV+DGELDPFIKSYLKHK+SMTMSTSGVN
Sbjct: 422 IRNYVFHPYKLVKDVRTGHETPDITSVIDGELDPFIKSYLKHKYSMTMSTSGVN 475


>Medtr7g112100.1 | peptide chain release factor 2 | HC |
           chr7:46081007-46085393 | 20130731
          Length = 475

 Score =  254 bits (650), Expect = 9e-68,   Method: Compositional matrix adjust.
 Identities = 142/325 (43%), Positives = 196/325 (60%), Gaps = 11/325 (3%)

Query: 127 FWDNRAKA----QETLSTLSDVKDKINLLAEFKTQVEDAETIVKLTEEMESSIDRALLEE 182
            WD+  +A    +E  S L  +K+   L  E    ++D E I    EE +S ++   LE 
Sbjct: 147 LWDDPVRAGKINREHGSLLGKMKEVNGLEREL---LDDIEMIKLAREENDSELE---LES 200

Query: 183 ATSLIKELNKSLDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYMRWGEK 242
             +L+     + ++ EL  LL+   D     I + AGAGGT++ DWA M+++MY  W ++
Sbjct: 201 MKALLNMRVNAKEK-ELEALLADEQDSRSCYIEVQAGAGGTESMDWASMIMQMYKSWAQR 259

Query: 243 QRYKTRVVERSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVRQSPFNSKGLRQTSFS 302
           + YK  V+E  PGE AGIK ATI+V+G +A+GY   E G HR+VR SPF+S   R TSF+
Sbjct: 260 RGYKVTVMEEMPGEIAGIKRATIKVDGEFAFGYAKAEIGVHRLVRISPFDSNKRRHTSFA 319

Query: 303 GVEVMPLLPEESLDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEE 362
            V V+P L +ES  V+I E DL I   RA G GGQ+VN  E+AV+ITHIPTGVT  C  E
Sbjct: 320 AVAVIPNLGDESSSVQINESDLRIERFRASGAGGQHVNTTESAVKITHIPTGVTATCQNE 379

Query: 363 RSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDV 422
           RSQ  NK  A++ L+++L  I   ++A    Q         WG QIR+YV HPY++VKD+
Sbjct: 380 RSQHQNKASAMAVLQSRLNQIEMARQAQLNAQHTQSLTDITWGSQIRSYVLHPYRMVKDL 439

Query: 423 RTGHETPDVTSVMDGELDPFIKSYL 447
           RT +E  D  SV++G+LD FI SYL
Sbjct: 440 RTNYEVSDPDSVLEGDLDGFIMSYL 464


>Medtr1g041890.1 | peptide chain release factor, putative | HC |
           chr1:20209058-20203032 | 20130731
          Length = 548

 Score =  253 bits (646), Expect = 3e-67,   Method: Compositional matrix adjust.
 Identities = 145/365 (39%), Positives = 217/365 (59%), Gaps = 8/365 (2%)

Query: 84  AFYSLRKDVEITSQRVEEIRESSGIXXXXXXXXXXXXKASATSFWDNRAKAQETLSTLSD 143
             +SLR+ +E T  R E +  SS +                +  WD+  K+ + L  L++
Sbjct: 189 GLFSLRRKIEDTVIRAE-LYASSALEMEEASWIKQEEMVRDSDLWDDPTKSNDILVKLAN 247

Query: 144 VKDKINLLAEFKTQVEDAETIVKLTEEMESSIDRALLEEATSLIKELNKSLDRFELTQLL 203
               ++ L + + +VE+A+ I +L E   ++ID  L ++A     +++  LD++E+++LL
Sbjct: 248 SAKVVDSLKDLRYKVEEAKLIKQLAEM--NAIDYGLYKQAYDASVDVSNILDQYEISKLL 305

Query: 204 SGPYDKEGAVISITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTRVVERSPGEEAGIKSA 263
            GP+D  GA + I A A G  ++ WA+ LL+MY+ W ++Q Y+ R+V+R   E  GI SA
Sbjct: 306 KGPFDMAGACLVIKA-ATGIFSKLWAEQLLQMYLGWAKRQGYEGRIVDRCLTENGGINSA 364

Query: 264 TIEVEGRYAYGYLSGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPLLPEESLDVEIPEED 323
           TIE E   AYGYL GEKG H ++R SP  S  L +TS + V+V+PL  E++LD+EI  ED
Sbjct: 365 TIEFEFECAYGYLLGEKGVHHLIRGSPNESSQL-ETSTATVDVIPLFLEKALDLEIDSED 423

Query: 324 LEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVI 383
           L IS     G+  +   ++   V I HIPTG+T++ + ERSQ ANK+KAL+RLKAKL VI
Sbjct: 424 LIISSPLIHGEQKRQTQRI---VCIHHIPTGITVQSSGERSQFANKMKALNRLKAKLQVI 480

Query: 384 AEEQRASEIKQIRGDAVKAEWGQQIRNYVFHPYKLVKDVRTGHETPDVTSVMDGELDPFI 443
           A EQ    I  I  D +   W ++ R YV HPYKLV DV+T  E  D+ SV+DG + P I
Sbjct: 481 AIEQGVGSINGIVKDKIVNLWEEKTRRYVSHPYKLVHDVKTDIEMSDLNSVLDGNIGPLI 540

Query: 444 KSYLK 448
            +++ 
Sbjct: 541 AAHIN 545


>Medtr7g110570.1 | peptide chain release factor 1 | HC |
           chr7:45311012-45315116 | 20130731
          Length = 438

 Score =  149 bits (376), Expect = 6e-36,   Method: Compositional matrix adjust.
 Identities = 108/322 (33%), Positives = 165/322 (51%), Gaps = 14/322 (4%)

Query: 132 AKAQETLSTLSDVKDKINLLAEFKTQVEDAETIVKLTEEMESSIDRALL----EEATSLI 187
           A+A + L  LS     IN L   + +++  ++++      ESS D+ +L    EE    I
Sbjct: 81  ARANKELRKLSGSVQLINQLKAKQKEIDGLKSLMN-----ESSEDKDMLNMATEEMGQAI 135

Query: 188 KELNKSLDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYMRWGEKQRYKT 247
           +E  K  +    + L     D+   ++ + AG GG +A  +A  + +MY ++   + +K 
Sbjct: 136 EEERKLQNLLLKSLLPKDDADERDCILEVRAGTGGEEASLFAMNIFKMYEKYAHNKGWKF 195

Query: 248 RVVERSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVRQSPFNSKGLRQTSFSGVEVM 307
            VV+ +  +  G K AT  + G   +G L  E G HR+ R       G   TS   V ++
Sbjct: 196 DVVDIAQSDMKGFKEATAAILGVGVFGKLKFESGIHRVQRVPVTEKLGRVHTSAVSVAIL 255

Query: 308 PLLPEESLDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLA 367
           P   E  +DV++ +EDL+I F R+GG GGQ+ NK  +AVR+THIPTG+ +   +ERSQ  
Sbjct: 256 PQADE--VDVQLKKEDLKIDFYRSGGSGGQHANKTSSAVRMTHIPTGIVIAIQDERSQHM 313

Query: 368 NKIKALSRLKAKLLVIAEEQRASEIKQIRGDAV-KAEWGQQIRNYVFHPYKLVKDVRTGH 426
           NK KAL  L AKL  I   +  S   ++R D +   +  ++IR Y F P   V D R G 
Sbjct: 314 NKAKALKVLCAKLYEIERTRLHSSRSKLRSDQIGSGDRSERIRTYNF-PQGRVTDHRVGI 372

Query: 427 ETPDVTSVMDGE-LDPFIKSYL 447
              ++  VM GE LD FI + L
Sbjct: 373 TYHNIDDVMQGESLDAFIDALL 394


>Medtr7g110570.2 | peptide chain release factor 1 | HC |
           chr7:45310948-45315157 | 20130731
          Length = 403

 Score =  145 bits (367), Expect = 6e-35,   Method: Compositional matrix adjust.
 Identities = 100/282 (35%), Positives = 147/282 (52%), Gaps = 9/282 (3%)

Query: 172 ESSIDRALL----EEATSLIKELNKSLDRFELTQLLSGPYDKEGAVISITAGAGGTDAQD 227
           ESS D+ +L    EE    I+E  K  +    + L     D+   ++ + AG GG +A  
Sbjct: 110 ESSEDKDMLNMATEEMGQAIEEERKLQNLLLKSLLPKDDADERDCILEVRAGTGGEEASL 169

Query: 228 WADMLLRMYMRWGEKQRYKTRVVERSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVR 287
           +A  + +MY ++   + +K  VV+ +  +  G K AT  + G   +G L  E G HR+ R
Sbjct: 170 FAMNIFKMYEKYAHNKGWKFDVVDIAQSDMKGFKEATAAILGVGVFGKLKFESGIHRVQR 229

Query: 288 QSPFNSKGLRQTSFSGVEVMPLLPEESLDVEIPEEDLEISFSRAGGKGGQNVNKVETAVR 347
                  G   TS   V ++P   E  +DV++ +EDL+I F R+GG GGQ+ NK  +AVR
Sbjct: 230 VPVTEKLGRVHTSAVSVAILPQADE--VDVQLKKEDLKIDFYRSGGSGGQHANKTSSAVR 287

Query: 348 ITHIPTGVTLRCTEERSQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAV-KAEWGQ 406
           +THIPTG+ +   +ERSQ  NK KAL  L AKL  I   +  S   ++R D +   +  +
Sbjct: 288 MTHIPTGIVIAIQDERSQHMNKAKALKVLCAKLYEIERTRLHSSRSKLRSDQIGSGDRSE 347

Query: 407 QIRNYVFHPYKLVKDVRTGHETPDVTSVMDGE-LDPFIKSYL 447
           +IR Y F P   V D R G    ++  VM GE LD FI + L
Sbjct: 348 RIRTYNF-PQGRVTDHRVGITYHNIDDVMQGESLDAFIDALL 388


>Medtr7g108300.1 | peptide chain release factor 1 | HC |
           chr7:44051484-44058335 | 20130731
          Length = 416

 Score =  145 bits (365), Expect = 1e-34,   Method: Compositional matrix adjust.
 Identities = 101/325 (31%), Positives = 178/325 (54%), Gaps = 15/325 (4%)

Query: 130 NRAKAQETLSTLSDVKDKINLLAEFKTQVEDAETIVKLTEEM--ESSIDRALLE----EA 183
           N ++ Q+   ++S++   ++L  +FK    D E  ++ T+ +  ++  D+ + E    E 
Sbjct: 80  NPSEYQKLAQSVSELDVVVSLYQKFK----DCEKTLEETQALAKDAGNDQDMTEMISYEI 135

Query: 184 TSLIKELNKSLDRFELTQLLSGPYDKEGAVISITAGAGGTDAQDWADMLLRMYMRWGEKQ 243
            SL K+L++  ++ ++  L S P D+   ++ + AG GG +A  WA  L+RMY ++ E  
Sbjct: 136 ESLSKQLSELEEKLKVLLLPSDPLDERNIMLEVRAGTGGDEAGIWAGDLVRMYEKFSELN 195

Query: 244 RYKTRVVERSPGEEAGIKSATIEVEGRYAYGYLSGEKGTHRIVRQSPFNSKGLRQTSFSG 303
            +K   +  S  E+ G K+  +E++G   Y  L  E G HR+ R     ++G   TS + 
Sbjct: 196 SWKHSAISCSEAEKGGYKTYVMEIKGNRVYSKLKFESGVHRVQRVPLTETQGRVHTSTAT 255

Query: 304 VEVMPLLPEESLDVEIPEEDLEISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEER 363
           V +MP + E  ++V I  +D+E++ +R+GG GGQNVNKVETA+ + H PTG+ + CTE+R
Sbjct: 256 VAIMPEVDE--VEVVIDPKDIEVTTARSGGAGGQNVNKVETAIDLVHKPTGIRIFCTEQR 313

Query: 364 SQLANKIKALSRLKAKLLVIAEEQRASEIKQIRGDAV-KAEWGQQIRNYVFHPYKLVKDV 422
           +Q+ NK  A   L+AKL  I   ++   I+  R   V      ++IR Y +   + V D 
Sbjct: 314 TQIQNKNLAFKLLRAKLYEIKVREQQESIRNQRKSQVGTGARSEKIRTYNYKDNR-VTDH 372

Query: 423 RTGHETPDVTSVMDGELDPFIKSYL 447
           R   +   +TS + G ++  ++S +
Sbjct: 373 RL-KQNFSLTSFLGGNIEEAVQSCV 396


>Medtr6g059330.1 | class I peptide chain release factor | HC |
           chr6:20369783-20372756 | 20130731
          Length = 250

 Score = 53.1 bits (126), Expect = 6e-07,   Method: Compositional matrix adjust.
 Identities = 25/66 (37%), Positives = 43/66 (65%), Gaps = 4/66 (6%)

Query: 317 VEIPEEDL----EISFSRAGGKGGQNVNKVETAVRITHIPTGVTLRCTEERSQLANKIKA 372
           +++ ++DL    E+   +  G GGQ+ NK E+AVR+ H+PTG+  +  E+RSQ  N+  A
Sbjct: 70  LDLTDDDLMRQCEMGTFKTSGPGGQHRNKRESAVRLKHLPTGIIAQAGEDRSQHMNRASA 129

Query: 373 LSRLKA 378
           + RL++
Sbjct: 130 IKRLRS 135