Miyakogusa Predicted Gene

Lj1g3v2627550.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v2627550.1 Non Characterized Hit- tr|I1N3M8|I1N3M8_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.54372
PE,81.25,0,TETRAHYDROFOLATE DEHYDROGENASE/CYCLOHYDROLASE FAMILY
MEMBER,NULL; NAD(P)-binding Rossmann-fold domai,CUFF.29360.1
         (352 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr7g076680.1 | methylenetetrahydrofolate dehydrogenase | HC |...   571   e-163
Medtr7g076680.3 | methylenetetrahydrofolate dehydrogenase | HC |...   565   e-161
Medtr7g076680.2 | methylenetetrahydrofolate dehydrogenase | HC |...   534   e-152
Medtr1g030450.1 | methylenetetrahydrofolate dehydrogenase | HC |...   396   e-110
Medtr7g108580.1 | cytoplasmic C-1-tetrahydrofolate synthase | HC...   395   e-110
Medtr3g007490.1 | methylenetetrahydrofolate dehydrogenase | HC |...   349   2e-96
Medtr3g007580.1 | methylenetetrahydrofolate dehydrogenase | HC |...   100   4e-21

>Medtr7g076680.1 | methylenetetrahydrofolate dehydrogenase | HC |
           chr7:28900731-28896287 | 20130731
          Length = 351

 Score =  571 bits (1471), Expect = e-163,   Method: Compositional matrix adjust.
 Identities = 285/353 (80%), Positives = 316/353 (89%), Gaps = 3/353 (0%)

Query: 1   MASTLFFTNCHLSPFSSTAAALRR-HPIRVAPTSLRFLALHSAPSQSSRVAAVAAMGTEA 59
           MAS+L  T+C+ SP S+T   LRR HPI V PTSLRF  +HS+PS S  ++  A+    +
Sbjct: 1   MASSL--THCYSSPSSTTTLLLRRRHPINVGPTSLRFFTIHSSPSSSRVLSIAASATEAS 58

Query: 60  SAKVIDGKLVAKQIRDEITAEVSRMKEAIGVVPGLAVILVGDRKDSATYVRNKQKACEAA 119
           SAKVIDGK VAKQIRDEI  EVSRM+EA GV+PGLAVILVGDRKDSATYVRNK+KACE  
Sbjct: 59  SAKVIDGKTVAKQIRDEIAVEVSRMREAAGVIPGLAVILVGDRKDSATYVRNKKKACETV 118

Query: 120 GINSLEAFLPEDSTEEEVLNYISGYNEDPSVHGILVQLPLPSHMNEQNILNAITIEKDVD 179
           GINSLE  LPEDSTEEEVLN+I+GYN+DPSVHGILVQLPLPSHMNEQN+LNA+ IEKDVD
Sbjct: 119 GINSLEVNLPEDSTEEEVLNHIAGYNDDPSVHGILVQLPLPSHMNEQNVLNAVRIEKDVD 178

Query: 180 GFHPLNIGRLAMRDREPLFVPCTPKGCIELLRRYGVSIKGTRAVVIGRSNIVGMPAALLL 239
           GFHPLNIGRLAMR REPLFVPCTPKGCIELL RYGVSIKG RAVVIGRSNIVGMPAALLL
Sbjct: 179 GFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYGVSIKGKRAVVIGRSNIVGMPAALLL 238

Query: 240 QREDATVSVVHSKTLNPEEIIRQADIVISAAGQPHMVRGSWIKPGAVIIDVGINPVEDPN 299
           QREDATVS+VHS+T NPEEIIRQADI+I+A GQP+MV+GSWIKPGAVIIDVGINPV+DP+
Sbjct: 239 QREDATVSIVHSRTTNPEEIIRQADIIIAAVGQPNMVKGSWIKPGAVIIDVGINPVDDPS 298

Query: 300 STRGYRLIGDVCYEEAIKVASAVTPVPGGVGPMTIAMLLQNTLNSAKRIHKFE 352
           S+RGY+L+GDVCYEEA+KVASA+TPVPGGVGPMTIAMLLQNTL SAKRIH FE
Sbjct: 299 SSRGYKLVGDVCYEEAVKVASAITPVPGGVGPMTIAMLLQNTLISAKRIHSFE 351


>Medtr7g076680.3 | methylenetetrahydrofolate dehydrogenase | HC |
           chr7:28900731-28896287 | 20130731
          Length = 368

 Score =  565 bits (1455), Expect = e-161,   Method: Compositional matrix adjust.
 Identities = 284/371 (76%), Positives = 315/371 (84%), Gaps = 22/371 (5%)

Query: 1   MASTLFFTNCHLSPFSSTAAALRR-HPIRVAPTSLRFLALHSAPSQSSRVAAVAAMGTEA 59
           MAS+L  T+C+ SP S+T   LRR HPI V PTSLRF  +HS+   SSRV ++ +     
Sbjct: 1   MASSL--THCYSSPSSTTTLLLRRRHPINVGPTSLRFFTIHSS-PSSSRVLSIGSYVARY 57

Query: 60  SA------------------KVIDGKLVAKQIRDEITAEVSRMKEAIGVVPGLAVILVGD 101
           +A                  KVIDGK VAKQIRDEI  EVSRM+EA GV+PGLAVILVGD
Sbjct: 58  NANFFSGAYMSASATEASSAKVIDGKTVAKQIRDEIAVEVSRMREAAGVIPGLAVILVGD 117

Query: 102 RKDSATYVRNKQKACEAAGINSLEAFLPEDSTEEEVLNYISGYNEDPSVHGILVQLPLPS 161
           RKDSATYVRNK+KACE  GINSLE  LPEDSTEEEVLN+I+GYN+DPSVHGILVQLPLPS
Sbjct: 118 RKDSATYVRNKKKACETVGINSLEVNLPEDSTEEEVLNHIAGYNDDPSVHGILVQLPLPS 177

Query: 162 HMNEQNILNAITIEKDVDGFHPLNIGRLAMRDREPLFVPCTPKGCIELLRRYGVSIKGTR 221
           HMNEQN+LNA+ IEKDVDGFHPLNIGRLAMR REPLFVPCTPKGCIELL RYGVSIKG R
Sbjct: 178 HMNEQNVLNAVRIEKDVDGFHPLNIGRLAMRGREPLFVPCTPKGCIELLHRYGVSIKGKR 237

Query: 222 AVVIGRSNIVGMPAALLLQREDATVSVVHSKTLNPEEIIRQADIVISAAGQPHMVRGSWI 281
           AVVIGRSNIVGMPAALLLQREDATVS+VHS+T NPEEIIRQADI+I+A GQP+MV+GSWI
Sbjct: 238 AVVIGRSNIVGMPAALLLQREDATVSIVHSRTTNPEEIIRQADIIIAAVGQPNMVKGSWI 297

Query: 282 KPGAVIIDVGINPVEDPNSTRGYRLIGDVCYEEAIKVASAVTPVPGGVGPMTIAMLLQNT 341
           KPGAVIIDVGINPV+DP+S+RGY+L+GDVCYEEA+KVASA+TPVPGGVGPMTIAMLLQNT
Sbjct: 298 KPGAVIIDVGINPVDDPSSSRGYKLVGDVCYEEAVKVASAITPVPGGVGPMTIAMLLQNT 357

Query: 342 LNSAKRIHKFE 352
           L SAKRIH FE
Sbjct: 358 LISAKRIHSFE 368


>Medtr7g076680.2 | methylenetetrahydrofolate dehydrogenase | HC |
           chr7:28900731-28896314 | 20130731
          Length = 347

 Score =  534 bits (1376), Expect = e-152,   Method: Compositional matrix adjust.
 Identities = 254/291 (87%), Positives = 275/291 (94%)

Query: 62  KVIDGKLVAKQIRDEITAEVSRMKEAIGVVPGLAVILVGDRKDSATYVRNKQKACEAAGI 121
           KVIDGK VAKQIRDEI  EVSRM+EA GV+PGLAVILVGDRKDSATYVRNK+KACE  GI
Sbjct: 57  KVIDGKTVAKQIRDEIAVEVSRMREAAGVIPGLAVILVGDRKDSATYVRNKKKACETVGI 116

Query: 122 NSLEAFLPEDSTEEEVLNYISGYNEDPSVHGILVQLPLPSHMNEQNILNAITIEKDVDGF 181
           NSLE  LPEDSTEEEVLN+I+GYN+DPSVHGILVQLPLPSHMNEQN+LNA+ IEKDVDGF
Sbjct: 117 NSLEVNLPEDSTEEEVLNHIAGYNDDPSVHGILVQLPLPSHMNEQNVLNAVRIEKDVDGF 176

Query: 182 HPLNIGRLAMRDREPLFVPCTPKGCIELLRRYGVSIKGTRAVVIGRSNIVGMPAALLLQR 241
           HPLNIGRLAMR REPLFVPCTPKGCIELL RYGVSIKG RAVVIGRSNIVGMPAALLLQR
Sbjct: 177 HPLNIGRLAMRGREPLFVPCTPKGCIELLHRYGVSIKGKRAVVIGRSNIVGMPAALLLQR 236

Query: 242 EDATVSVVHSKTLNPEEIIRQADIVISAAGQPHMVRGSWIKPGAVIIDVGINPVEDPNST 301
           EDATVS+VHS+T NPEEIIRQADI+I+A GQP+MV+GSWIKPGAVIIDVGINPV+DP+S+
Sbjct: 237 EDATVSIVHSRTTNPEEIIRQADIIIAAVGQPNMVKGSWIKPGAVIIDVGINPVDDPSSS 296

Query: 302 RGYRLIGDVCYEEAIKVASAVTPVPGGVGPMTIAMLLQNTLNSAKRIHKFE 352
           RGY+L+GDVCYEEA+KVASA+TPVPGGVGPMTIAMLLQNTL SAKRIH FE
Sbjct: 297 RGYKLVGDVCYEEAVKVASAITPVPGGVGPMTIAMLLQNTLISAKRIHSFE 347


>Medtr1g030450.1 | methylenetetrahydrofolate dehydrogenase | HC |
           chr1:10595287-10589370 | 20130731
          Length = 361

 Score =  396 bits (1017), Expect = e-110,   Method: Compositional matrix adjust.
 Identities = 193/322 (59%), Positives = 241/322 (74%)

Query: 30  APTSLRFLALHSAPSQSSRVAAVAAMGTEASAKVIDGKLVAKQIRDEITAEVSRMKEAIG 89
           A  SL    + +  S    V  +     E +A VIDGKL+A +IR +I  +V +MK+ +G
Sbjct: 39  ALASLDLPDIWTPNSSCQSVPDIHKSFNEQTATVIDGKLMATEIRSKIADQVRQMKKGLG 98

Query: 90  VVPGLAVILVGDRKDSATYVRNKQKACEAAGINSLEAFLPEDSTEEEVLNYISGYNEDPS 149
            VPGLAVILVG R+DS TYVRNK  ACE  G+ S+   LP D  + +V N I  +N+DPS
Sbjct: 99  KVPGLAVILVGQRRDSLTYVRNKIIACEEVGMKSVVTELPTDCADADVQNAIMRFNKDPS 158

Query: 150 VHGILVQLPLPSHMNEQNILNAITIEKDVDGFHPLNIGRLAMRDREPLFVPCTPKGCIEL 209
           +HGILVQLPLP H++E+ IL+A+ +EKDVDGFHP+N+G LA+R REPLF+PCT K CIEL
Sbjct: 159 IHGILVQLPLPQHLDEEKILDAVCLEKDVDGFHPVNMGNLALRGREPLFIPCTTKACIEL 218

Query: 210 LRRYGVSIKGTRAVVIGRSNIVGMPAALLLQREDATVSVVHSKTLNPEEIIRQADIVISA 269
           L R GV I G +AVVIGRSNIVG+P +LLLQR  ATV+VVH+ T NPE+I  +ADIV+SA
Sbjct: 219 LIRSGVEIMGKKAVVIGRSNIVGLPTSLLLQRHHATVTVVHAFTENPEQITSEADIVVSA 278

Query: 270 AGQPHMVRGSWIKPGAVIIDVGINPVEDPNSTRGYRLIGDVCYEEAIKVASAVTPVPGGV 329
           AG P++V G+WIK GA +IDVG  PVEDP+   GYRL+GDVC+EE IKVAS +TPVPGGV
Sbjct: 279 AGVPNLVCGNWIKSGATVIDVGTIPVEDPSCEDGYRLVGDVCFEEVIKVASVITPVPGGV 338

Query: 330 GPMTIAMLLQNTLNSAKRIHKF 351
           GPMT+ MLL NTL+SAKR+  F
Sbjct: 339 GPMTVTMLLVNTLDSAKRLFNF 360


>Medtr7g108580.1 | cytoplasmic C-1-tetrahydrofolate synthase | HC |
           chr7:44234483-44237478 | 20130731
          Length = 293

 Score =  395 bits (1015), Expect = e-110,   Method: Compositional matrix adjust.
 Identities = 186/287 (64%), Positives = 228/287 (79%)

Query: 61  AKVIDGKLVAKQIRDEITAEVSRMKEAIGVVPGLAVILVGDRKDSATYVRNKQKACEAAG 120
           A VIDGK VA+ IR EIT EV  + E  G VPGLAV++VG+R+DS +YVR K+KAC   G
Sbjct: 2   ATVIDGKAVAQTIRSEITDEVRLLSEKYGKVPGLAVVIVGNRQDSESYVRTKRKACADLG 61

Query: 121 INSLEAFLPEDSTEEEVLNYISGYNEDPSVHGILVQLPLPSHMNEQNILNAITIEKDVDG 180
           I + +  LPE  +E EV+ ++   N DP+VHGILVQLPLP H+NE+ +L  I++EKDVDG
Sbjct: 62  IKNFDIDLPEQISEAEVIKHVHQLNADPNVHGILVQLPLPKHINEEKVLTEISLEKDVDG 121

Query: 181 FHPLNIGRLAMRDREPLFVPCTPKGCIELLRRYGVSIKGTRAVVIGRSNIVGMPAALLLQ 240
           FHPLNIG+LAM+ R+PLFVPCTPK C+ELL R GVSIKG RAVV+GRSNIVG+P +LLL 
Sbjct: 122 FHPLNIGKLAMKGRDPLFVPCTPKACLELLSRSGVSIKGKRAVVVGRSNIVGLPVSLLLL 181

Query: 241 REDATVSVVHSKTLNPEEIIRQADIVISAAGQPHMVRGSWIKPGAVIIDVGINPVEDPNS 300
           + DATV++ HS T  PE+IIR+ADIVI+AAGQ  M++GSWIKPGA +IDVG N V+DP  
Sbjct: 182 KADATVTIAHSHTSQPEDIIREADIVIAAAGQAKMIKGSWIKPGAAVIDVGTNSVDDPTR 241

Query: 301 TRGYRLIGDVCYEEAIKVASAVTPVPGGVGPMTIAMLLQNTLNSAKR 347
             GYRL+GDV +EEA KVA  +TPVPGGVGPMT+ MLL+NTL SAKR
Sbjct: 242 KAGYRLVGDVDFEEASKVAGWITPVPGGVGPMTVTMLLKNTLESAKR 288


>Medtr3g007490.1 | methylenetetrahydrofolate dehydrogenase | HC |
           chr3:1040089-1043268 | 20130731
          Length = 363

 Score =  349 bits (896), Expect = 2e-96,   Method: Compositional matrix adjust.
 Identities = 160/290 (55%), Positives = 220/290 (75%), Gaps = 1/290 (0%)

Query: 58  EASAKVIDGKLVAKQIRDEITAEVSRMKEAIGVVPGLAVILVGDRKDSATYVRNKQKACE 117
           + SA +++GK +A  I+ ++  E+ RMK  IG  P LA +LVGDR+DS T++  K KACE
Sbjct: 70  DHSAAILEGKPIANHIKLKVADEIRRMKSDIGKFPKLAFVLVGDRRDSHTFIHLKSKACE 129

Query: 118 AAGINSLEAFLPEDSTEEEVLNYISGYNEDPSVHGILVQLPLPSHMNEQNILNAITIEKD 177
             GI ++ + LPE+ TE E+L+ +SG N+D  VHGI+VQLPLP H++E+ I+N ++ EKD
Sbjct: 130 KVGIETVVSQLPENCTENELLDVVSGLNDDQDVHGIIVQLPLPQHLDEERIMNIVSPEKD 189

Query: 178 VDGFHPLNIGRLAMRDREPLFVPCTPKGCIELLRRYGVSIKGTRAVVIGRSNIVGMPAAL 237
           VDGFHPLNIG LA   R+P F+PC  + CIELL  +G+ I+G R  +IGR  I G+P +L
Sbjct: 190 VDGFHPLNIGNLARSGRKPFFIPCASRSCIELLLNHGIEIRGKRVAIIGRGKIAGLPTSL 249

Query: 238 LLQREDATVSVVHSKTLNPEEIIRQADIVISAAGQPHMVRGSWIKPGAVIIDVGINPVED 297
           LLQR  ATVS++H+ T NPE I  +ADIV++  G P++VRG WIK GAV+ID+G N V+D
Sbjct: 250 LLQRHHATVSLLHAYTKNPERITSEADIVVTDVGIPNIVRGDWIKKGAVVIDMGTNQVKD 309

Query: 298 PNSTRGYRLIGDVCYEEAIKVASAVTPVPGGVGPMTIAMLLQNTLNSAKR 347
           P S++ +R+ GDVC+EEA+KVASA+TPVPGG+GP+T++MLL NTL+SAKR
Sbjct: 310 P-SSQVFRVTGDVCFEEAVKVASAITPVPGGIGPVTVSMLLSNTLDSAKR 358


>Medtr3g007580.1 | methylenetetrahydrofolate dehydrogenase | HC |
           chr3:1065282-1067045 | 20130731
          Length = 134

 Score = 99.8 bits (247), Expect = 4e-21,   Method: Compositional matrix adjust.
 Identities = 51/111 (45%), Positives = 72/111 (64%), Gaps = 7/111 (6%)

Query: 186 IGRLAMRDREPLFVPCTPKGCIELLR-RYGVSIKGTRAVVIGRSNIVGMPAALLLQREDA 244
           I  LA   R+P F+PC  K CIELL   +G+ I+G +  +IGR  IVG+PA+LL+Q    
Sbjct: 13  IRNLARSGRKPFFIPCASKSCIELLLLNHGIEIRGKKVAIIGRDKIVGLPASLLMQ---- 68

Query: 245 TVSVVHSKTLNPEEIIRQADIVISAAGQPHMVRGSWIKPGAVIIDVGINPV 295
           + S +H +  N E+I  +A+IV++  G  ++VRG WIK GAV+ID G N V
Sbjct: 69  SASYMHKQ--NSEQITSEANIVVTYIGIRNIVRGDWIKKGAVVIDTGTNQV 117