Miyakogusa Predicted Gene
- Lj1g3v1901630.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v1901630.1 Non Characterized Hit- tr|I1K8U7|I1K8U7_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.34072 PE,70.85,0,FAMILY
NOT NAMED,NULL; TPX2,Xklp2 targeting protein; seg,NULL,CUFF.28132.1
(320 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr3g107050.1 | TPX2 (targeting protein for Xklp2) family prot... 331 6e-91
Medtr3g107050.2 | TPX2 (targeting protein for Xklp2) family prot... 331 6e-91
Medtr1g012530.1 | TPX2 (targeting protein for Xklp2) family prot... 315 3e-86
Medtr1g012530.2 | TPX2 (targeting protein for Xklp2) family prot... 284 1e-76
Medtr1g055085.1 | TPX2 (targeting protein for Xklp2) family prot... 146 3e-35
Medtr7g106750.1 | targeting protein for Xklp2 protein | HC | chr... 129 3e-30
Medtr1g075310.1 | TPX2 (targeting protein for Xklp2) family prot... 122 6e-28
Medtr1g075310.2 | TPX2 (targeting protein for Xklp2) family prot... 122 6e-28
Medtr1g080690.1 | TPX2 (targeting protein for Xklp2) family prot... 104 1e-22
Medtr7g115310.1 | FAD/NAD(P)-binding oxidoreductase family prote... 93 4e-19
Medtr6g022230.1 | TPX2 (targeting protein for Xklp2) family prot... 56 4e-08
>Medtr3g107050.1 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr3:49471851-49476541 | 20130731
Length = 453
Score = 331 bits (849), Expect = 6e-91, Method: Compositional matrix adjust.
Identities = 190/323 (58%), Positives = 226/323 (69%), Gaps = 24/323 (7%)
Query: 1 MDHSNLDHEDGLQAVHQNGGREEQPNFVEDGVVSNGDSVVAEISETVIPNGSFGNFNQLD 60
MD N EDGL HQ ++ N +D V N VV +I +TV+ NG NFN
Sbjct: 1 MDPVNTSLEDGL---HQ----QQLFNSQQDAVDFN---VVTQIKQTVLSNG---NFN--- 44
Query: 61 STATDNLSMTEIKE-GLDDTIYGNNVSISKXXXXXXXXXXXQARXXXXXXXXXXXXXXXX 119
DN+SM E +E G + I GNNV++SK ++R
Sbjct: 45 ----DNVSMEEEEEEGSNGKIEGNNVNVSKEVEIEIVDETEKSRTKKDLVKNNNSKLPSP 100
Query: 120 RGVHVSSLKKSKDGKEKEAA--SAVSNGTLAVDSHKRQPIKTKSFNDKQTRLSKHPGKSD 177
RG+ ++S++K+KDGK++EAA S+VSNGT DSH RQP+ ++ NDKQT LSKH GK+D
Sbjct: 101 RGLRMTSVRKNKDGKDEEAAVASSVSNGTSTFDSHPRQPVNNRAVNDKQTHLSKHSGKTD 160
Query: 178 AASSEAPVEKTRPQLLKKGPLDNQ-GKEEPSSPTAEDDKPGRAGALPNYGFSFKCDERAQ 236
AAS+EAP+EKTRP L+KK PLDN GK E S PT+ED KP R G +P YGFSFKC+ERA+
Sbjct: 161 AASTEAPMEKTRPHLIKKEPLDNLPGKAESSFPTSEDAKPRRVGTMPTYGFSFKCNERAE 220
Query: 237 RRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPSFYQEPPPPR 296
RR+EFYSKLEE+IHAKEVEES IQAKTKESQEAEIK+LRKKLAFKATPMPSFYQEP P R
Sbjct: 221 RRKEFYSKLEERIHAKEVEESNIQAKTKESQEAEIKRLRKKLAFKATPMPSFYQEPTPSR 280
Query: 297 VELKKIPTTRAKSPKLGRNKSST 319
VELKKIPTTRAKSPKLGR KSST
Sbjct: 281 VELKKIPTTRAKSPKLGRKKSST 303
>Medtr3g107050.2 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr3:49472302-49476481 | 20130731
Length = 453
Score = 331 bits (849), Expect = 6e-91, Method: Compositional matrix adjust.
Identities = 190/323 (58%), Positives = 226/323 (69%), Gaps = 24/323 (7%)
Query: 1 MDHSNLDHEDGLQAVHQNGGREEQPNFVEDGVVSNGDSVVAEISETVIPNGSFGNFNQLD 60
MD N EDGL HQ ++ N +D V N VV +I +TV+ NG NFN
Sbjct: 1 MDPVNTSLEDGL---HQ----QQLFNSQQDAVDFN---VVTQIKQTVLSNG---NFN--- 44
Query: 61 STATDNLSMTEIKE-GLDDTIYGNNVSISKXXXXXXXXXXXQARXXXXXXXXXXXXXXXX 119
DN+SM E +E G + I GNNV++SK ++R
Sbjct: 45 ----DNVSMEEEEEEGSNGKIEGNNVNVSKEVEIEIVDETEKSRTKKDLVKNNNSKLPSP 100
Query: 120 RGVHVSSLKKSKDGKEKEAA--SAVSNGTLAVDSHKRQPIKTKSFNDKQTRLSKHPGKSD 177
RG+ ++S++K+KDGK++EAA S+VSNGT DSH RQP+ ++ NDKQT LSKH GK+D
Sbjct: 101 RGLRMTSVRKNKDGKDEEAAVASSVSNGTSTFDSHPRQPVNNRAVNDKQTHLSKHSGKTD 160
Query: 178 AASSEAPVEKTRPQLLKKGPLDNQ-GKEEPSSPTAEDDKPGRAGALPNYGFSFKCDERAQ 236
AAS+EAP+EKTRP L+KK PLDN GK E S PT+ED KP R G +P YGFSFKC+ERA+
Sbjct: 161 AASTEAPMEKTRPHLIKKEPLDNLPGKAESSFPTSEDAKPRRVGTMPTYGFSFKCNERAE 220
Query: 237 RRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPSFYQEPPPPR 296
RR+EFYSKLEE+IHAKEVEES IQAKTKESQEAEIK+LRKKLAFKATPMPSFYQEP P R
Sbjct: 221 RRKEFYSKLEERIHAKEVEESNIQAKTKESQEAEIKRLRKKLAFKATPMPSFYQEPTPSR 280
Query: 297 VELKKIPTTRAKSPKLGRNKSST 319
VELKKIPTTRAKSPKLGR KSST
Sbjct: 281 VELKKIPTTRAKSPKLGRKKSST 303
>Medtr1g012530.1 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr1:2520568-2514968 | 20130731
Length = 459
Score = 315 bits (808), Expect = 3e-86, Method: Compositional matrix adjust.
Identities = 179/320 (55%), Positives = 223/320 (69%), Gaps = 2/320 (0%)
Query: 1 MDHSNLDHEDGLQAVHQNGGREEQPNFVEDGVVSNGDSVVAEISETVIPNGSFGNFNQLD 60
MD N DGL VHQNG +E N ED V ++ D V +ET +P+G+ N NQL+
Sbjct: 2 MDPVNSLPADGLDDVHQNGVHDEPSNSGEDAVSNDLDPHVTVNTETFVPDGNSENINQLE 61
Query: 61 STATDNLSMTEIKEGLDDTIYGNNVSISKXXXXXXXXXXXQARXXXXXXXXXXXXXXXXR 120
STAT N +M EI EG +D + G+N+++SK Q+R
Sbjct: 62 STATGNSAMKEI-EGSNDNVDGSNLTVSKEKEVKIKVSTEQSRAQKGPVKNKNAKVGSSS 120
Query: 121 GVHVSSLKKSKDGKEKEAASAVSNGTLAVDSHKRQPIKTKSFNDKQTRLSKHPGKSDAAS 180
GV+ S +K SK GK+K+A+ AVSNGT A+DS RQPIK +S ND+Q++LSK P KS+AAS
Sbjct: 121 GVNASLVKNSKIGKDKQASPAVSNGTSALDSRPRQPIKNRSSNDRQSQLSKQPSKSEAAS 180
Query: 181 SEAPVEKTRPQLLKKGPLDN-QGKEEPSSPTAEDDKPGRAGALPNYGFSFKCDERAQRRR 239
S+ VEK +P+ LKKGPLD QG+ E S ED KP R G LPNYGFSF+C ERA++RR
Sbjct: 181 SDVAVEKKKPKSLKKGPLDKVQGEGESSLTNREDTKPRRVGTLPNYGFSFRCGERAEKRR 240
Query: 240 EFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPSFYQEPPPPRVEL 299
EF +K+EEKI AKE E+S++QAK+KESQEAEIKKLRK L FKATP+P+FYQEP PP+VEL
Sbjct: 241 EFLTKVEEKIQAKEEEKSSLQAKSKESQEAEIKKLRKSLTFKATPLPTFYQEPAPPKVEL 300
Query: 300 KKIPTTRAKSPKLGRNKSST 319
KKIPTTRAKSPKLGR K+ST
Sbjct: 301 KKIPTTRAKSPKLGRKKTST 320
>Medtr1g012530.2 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr1:2520543-2514968 | 20130731
Length = 347
Score = 284 bits (726), Expect = 1e-76, Method: Compositional matrix adjust.
Identities = 165/315 (52%), Positives = 211/315 (66%), Gaps = 2/315 (0%)
Query: 1 MDHSNLDHEDGLQAVHQNGGREEQPNFVEDGVVSNGDSVVAEISETVIPNGSFGNFNQLD 60
MD N DGL VHQNG +E N ED V ++ D V +ET +P+G+ N NQL+
Sbjct: 2 MDPVNSLPADGLDDVHQNGVHDEPSNSGEDAVSNDLDPHVTVNTETFVPDGNSENINQLE 61
Query: 61 STATDNLSMTEIKEGLDDTIYGNNVSISKXXXXXXXXXXXQARXXXXXXXXXXXXXXXXR 120
STAT N +M EI EG +D + G+N+++SK Q+R
Sbjct: 62 STATGNSAMKEI-EGSNDNVDGSNLTVSKEKEVKIKVSTEQSRAQKGPVKNKNAKVGSSS 120
Query: 121 GVHVSSLKKSKDGKEKEAASAVSNGTLAVDSHKRQPIKTKSFNDKQTRLSKHPGKSDAAS 180
GV+ S +K SK GK+K+A+ AVSNGT A+DS RQPIK +S ND+Q++LSK P KS+AAS
Sbjct: 121 GVNASLVKNSKIGKDKQASPAVSNGTSALDSRPRQPIKNRSSNDRQSQLSKQPSKSEAAS 180
Query: 181 SEAPVEKTRPQLLKKGPLDN-QGKEEPSSPTAEDDKPGRAGALPNYGFSFKCDERAQRRR 239
S+ VEK +P+ LKKGPLD QG+ E S ED KP R G LPNYGFSF+C ERA++RR
Sbjct: 181 SDVAVEKKKPKSLKKGPLDKVQGEGESSLTNREDTKPRRVGTLPNYGFSFRCGERAEKRR 240
Query: 240 EFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPSFYQEPPPPRVEL 299
EF +K+EEKI AKE E+S++QAK+KESQEAEIKKLRK L FKATP+P+FYQEP PP+VEL
Sbjct: 241 EFLTKVEEKIQAKEEEKSSLQAKSKESQEAEIKKLRKSLTFKATPLPTFYQEPAPPKVEL 300
Query: 300 KKIPTTRAKSPKLGR 314
KK+ + + L R
Sbjct: 301 KKVNLSPSVCACLCR 315
>Medtr1g055085.1 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr1:24263571-24261070 | 20130731
Length = 452
Score = 146 bits (368), Expect = 3e-35, Method: Compositional matrix adjust.
Identities = 70/93 (75%), Positives = 81/93 (87%), Gaps = 1/93 (1%)
Query: 226 GFSFKCDERAQRRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPM 285
GFSF+ +ERA++RREF+SKLEEKI KE E S +Q K+KESQEAEIKKLRK++ FKA PM
Sbjct: 233 GFSFRLEERAEKRREFFSKLEEKIQEKEAEISNLQEKSKESQEAEIKKLRKRMTFKAAPM 292
Query: 286 PSFYQEPPPPRVELKKIPTTRAKSPKLGRNKSS 318
PSFY+E PPP+VELKKIPTTR KSPKLGRNK S
Sbjct: 293 PSFYKE-PPPKVELKKIPTTRPKSPKLGRNKGS 324
>Medtr7g106750.1 | targeting protein for Xklp2 protein | HC |
chr7:43471923-43475667 | 20130731
Length = 421
Score = 129 bits (324), Expect = 3e-30, Method: Compositional matrix adjust.
Identities = 62/93 (66%), Positives = 80/93 (86%), Gaps = 1/93 (1%)
Query: 226 GFSFKCDERAQRRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPM 285
GFS + +ERA++R+EF+SK+EEK+ AKE E++ QAK+KE+QE EIK+LRK L FKA PM
Sbjct: 238 GFSSRLEERAEKRKEFFSKVEEKVLAKEAEKTNQQAKSKENQEKEIKQLRKSLTFKAAPM 297
Query: 286 PSFYQEPPPPRVELKKIPTTRAKSPKLGRNKSS 318
PSFY+E PPP+VELKKIPTTR +SPKLGR++ S
Sbjct: 298 PSFYKE-PPPKVELKKIPTTRPRSPKLGRHRES 329
>Medtr1g075310.1 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr1:33342141-33338595 | 20130731
Length = 383
Score = 122 bits (305), Expect = 6e-28, Method: Compositional matrix adjust.
Identities = 59/92 (64%), Positives = 75/92 (81%)
Query: 228 SFKCDERAQRRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPS 287
SF+ ERAQRR+EFYSKLEEK A E E++ +A++KE +E IK+LR+ L FKA+PMPS
Sbjct: 217 SFRSTERAQRRKEFYSKLEEKQQAMEAEKNQNEARSKEEKEEAIKQLRRSLKFKASPMPS 276
Query: 288 FYQEPPPPRVELKKIPTTRAKSPKLGRNKSST 319
FY E PPP+V+LKK+P TRAKSPKLGR KS++
Sbjct: 277 FYHEGPPPKVDLKKLPPTRAKSPKLGRRKSNS 308
>Medtr1g075310.2 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr1:33341243-33338595 | 20130731
Length = 383
Score = 122 bits (305), Expect = 6e-28, Method: Compositional matrix adjust.
Identities = 59/92 (64%), Positives = 75/92 (81%)
Query: 228 SFKCDERAQRRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPS 287
SF+ ERAQRR+EFYSKLEEK A E E++ +A++KE +E IK+LR+ L FKA+PMPS
Sbjct: 217 SFRSTERAQRRKEFYSKLEEKQQAMEAEKNQNEARSKEEKEEAIKQLRRSLKFKASPMPS 276
Query: 288 FYQEPPPPRVELKKIPTTRAKSPKLGRNKSST 319
FY E PPP+V+LKK+P TRAKSPKLGR KS++
Sbjct: 277 FYHEGPPPKVDLKKLPPTRAKSPKLGRRKSNS 308
>Medtr1g080690.1 | TPX2 (targeting protein for Xklp2) family protein
| HC | chr1:35884532-35888074 | 20130731
Length = 369
Score = 104 bits (260), Expect = 1e-22, Method: Compositional matrix adjust.
Identities = 52/92 (56%), Positives = 65/92 (70%)
Query: 228 SFKCDERAQRRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPS 287
+F+ ERA +RREFY KLEEK A E+S +A+ KE QEA IK++RK L KA P+P+
Sbjct: 195 TFRSSERAAKRREFYLKLEEKNRALLEEKSQYEARLKEEQEAAIKQMRKNLVIKAKPVPN 254
Query: 288 FYQEPPPPRVELKKIPTTRAKSPKLGRNKSST 319
FY E PPP+ ELKK+P TR KSPKL N+ T
Sbjct: 255 FYYEAPPPKAELKKLPLTRPKSPKLNLNRRRT 286
>Medtr7g115310.1 | FAD/NAD(P)-binding oxidoreductase family protein
| HC | chr7:47633584-47625724 | 20130731
Length = 867
Score = 92.8 bits (229), Expect = 4e-19, Method: Compositional matrix adjust.
Identities = 52/90 (57%), Positives = 65/90 (72%)
Query: 228 SFKCDERAQRRREFYSKLEEKIHAKEVEESTIQAKTKESQEAEIKKLRKKLAFKATPMPS 287
+F+ ERA++R+EFY KLEEK A E E +A+ KE +EA +K+LRK L KA P+PS
Sbjct: 212 TFRIYERAEKRKEFYMKLEEKNRALEEERLQYEARRKEEEEAALKQLRKSLVIKAKPVPS 271
Query: 288 FYQEPPPPRVELKKIPTTRAKSPKLGRNKS 317
FY E PPP+ ELKK+P TR KSPKL R KS
Sbjct: 272 FYYEGPPPKTELKKLPLTRPKSPKLSRRKS 301
>Medtr6g022230.1 | TPX2 (targeting protein for Xklp2) family
protein, putative | HC | chr6:7750365-7745249 | 20130731
Length = 572
Score = 56.2 bits (134), Expect = 4e-08, Method: Compositional matrix adjust.
Identities = 35/90 (38%), Positives = 53/90 (58%), Gaps = 5/90 (5%)
Query: 227 FSFKCDERAQRRREFYSKLEEKIHAKEVEE-STIQAKTKESQEAEIK-KLRKKLAFKATP 284
FS + +ERA RR++ KLEEK +A E ++ + K KE +EI+ KLR+ F+A P
Sbjct: 416 FSLRTEERAARRKK---KLEEKFNANEEQKVQQLHTKLKEKTGSEIRRKLRQSFCFQARP 472
Query: 285 MPSFYQEPPPPRVELKKIPTTRAKSPKLGR 314
+P FY+E +E +K+ T +S GR
Sbjct: 473 LPDFYKERKASNIETRKVIQTHYESSNEGR 502