Miyakogusa Predicted Gene

Lj1g3v1560060.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v1560060.1 tr|G7J535|G7J535_MEDTR MscS family inner membrane
protein ynaI OS=Medicago truncatula GN=MTR_3g10028,83.4,0,seg,NULL;
Sm-like ribonucleoproteins,Like-Sm (LSM) domain;
MS_channel,Mechanosensitive ion channel M,CUFF.27580.1
         (468 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g100280.2 | mechanosensitive ion channel-like protein | HC...   734   0.0  
Medtr3g100280.3 | mechanosensitive ion channel-like protein | HC...   734   0.0  
Medtr3g100280.1 | mechanosensitive ion channel-like protein | HC...   734   0.0  
Medtr5g032090.1 | mechanosensitive ion channel-like protein | HC...   476   e-134
Medtr3g100320.1 | mechanosensitive ion channel-like protein | HC...   418   e-117
Medtr3g100320.2 | mechanosensitive ion channel-like protein | HC...   269   5e-72
Medtr3g100300.1 | transmembrane protein, putative | LC | chr3:46...    94   2e-19
Medtr3g099260.1 | hypothetical protein | LC | chr3:45499769-4549...    75   1e-13

>Medtr3g100280.2 | mechanosensitive ion channel-like protein | HC |
           chr3:46095570-46102000 | 20130731
          Length = 553

 Score =  734 bits (1896), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 373/476 (78%), Positives = 395/476 (82%), Gaps = 8/476 (1%)

Query: 1   MGLDFAGKXXXXXXXXXXXXLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 60
           MGLDFAGK            LFMELLGFSTQKW               REIFTNFLSSIM
Sbjct: 78  MGLDFAGKAIYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSIM 137

Query: 61  IHATRPFILNEWIQTKIEGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVNVVRNL 120
           IH TRPF+LNEWIQTKIEGYEVSGTVEHVGWWSPTI+RGDDREAVHIPNHKFTVNVVRNL
Sbjct: 138 IHTTRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVNVVRNL 197

Query: 121 SQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSL 180
           +QK+HWRIKSYI+ISHLDVNKIN IVADMRKVLAKNPQVEQQ+LHRRVFLE++NPEN +L
Sbjct: 198 TQKSHWRIKSYISISHLDVNKINNIVADMRKVLAKNPQVEQQKLHRRVFLEDINPENTAL 257

Query: 181 MILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYREAESENIPFG 240
            ILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIY E  SENIPFG
Sbjct: 258 KILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYSEPASENIPFG 317

Query: 241 DTIFSRSRAAVNRPFLLIEPQYKVNGEDKVKPSTRSTSGNEEKDAKLDEIVVSDSNGDDN 300
           D+IF+RSRAAVNRPFLLIEP YKVNGEDK KPSTRST GNEEKDAK+DE V SDS  D+N
Sbjct: 318 DSIFTRSRAAVNRPFLLIEPPYKVNGEDKAKPSTRSTRGNEEKDAKVDEPVASDSKSDEN 377

Query: 301 LAATSTSPPGVNSKDKSKSISGVPNQNMGSDSSVEKTSKTMQPKKESAGDVRKGSAVPAS 360
            A TSTSP  VNSKDKSKS S    QNMGSDSSVEKTSKTMQPKKE+AGDV KGS +P  
Sbjct: 378 FAGTSTSPSSVNSKDKSKSKSDAQTQNMGSDSSVEKTSKTMQPKKETAGDVGKGSTIPVP 437

Query: 361 KNLSQSAVPEISPV-TSHE-------SGTASQSKQDGEKSSVTSSPVRSPLEENILLEVA 412
           K  + S V E  PV T+HE       S T+SQSKQD EKSSV SS VR+PLEENILL VA
Sbjct: 438 KTPAHSVVSETLPVITNHESSRADTASATSSQSKQDEEKSSVPSSAVRTPLEENILLGVA 497

Query: 413 LEGSKRTLPIEEEMTSSPTPAESQEFAVQRNGSGPPSNKDKKDGPISSFPTTKQNE 468
           LEGSKRTLPIEEEM  SP  AESQEFAVQRNG+GPP+NKDKKDGP SSFP  KQN+
Sbjct: 498 LEGSKRTLPIEEEMNPSPNSAESQEFAVQRNGNGPPANKDKKDGPTSSFPNAKQND 553


>Medtr3g100280.3 | mechanosensitive ion channel-like protein | HC |
           chr3:46095515-46102000 | 20130731
          Length = 553

 Score =  734 bits (1896), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 373/476 (78%), Positives = 395/476 (82%), Gaps = 8/476 (1%)

Query: 1   MGLDFAGKXXXXXXXXXXXXLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 60
           MGLDFAGK            LFMELLGFSTQKW               REIFTNFLSSIM
Sbjct: 78  MGLDFAGKAIYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSIM 137

Query: 61  IHATRPFILNEWIQTKIEGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVNVVRNL 120
           IH TRPF+LNEWIQTKIEGYEVSGTVEHVGWWSPTI+RGDDREAVHIPNHKFTVNVVRNL
Sbjct: 138 IHTTRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVNVVRNL 197

Query: 121 SQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSL 180
           +QK+HWRIKSYI+ISHLDVNKIN IVADMRKVLAKNPQVEQQ+LHRRVFLE++NPEN +L
Sbjct: 198 TQKSHWRIKSYISISHLDVNKINNIVADMRKVLAKNPQVEQQKLHRRVFLEDINPENTAL 257

Query: 181 MILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYREAESENIPFG 240
            ILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIY E  SENIPFG
Sbjct: 258 KILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYSEPASENIPFG 317

Query: 241 DTIFSRSRAAVNRPFLLIEPQYKVNGEDKVKPSTRSTSGNEEKDAKLDEIVVSDSNGDDN 300
           D+IF+RSRAAVNRPFLLIEP YKVNGEDK KPSTRST GNEEKDAK+DE V SDS  D+N
Sbjct: 318 DSIFTRSRAAVNRPFLLIEPPYKVNGEDKAKPSTRSTRGNEEKDAKVDEPVASDSKSDEN 377

Query: 301 LAATSTSPPGVNSKDKSKSISGVPNQNMGSDSSVEKTSKTMQPKKESAGDVRKGSAVPAS 360
            A TSTSP  VNSKDKSKS S    QNMGSDSSVEKTSKTMQPKKE+AGDV KGS +P  
Sbjct: 378 FAGTSTSPSSVNSKDKSKSKSDAQTQNMGSDSSVEKTSKTMQPKKETAGDVGKGSTIPVP 437

Query: 361 KNLSQSAVPEISPV-TSHE-------SGTASQSKQDGEKSSVTSSPVRSPLEENILLEVA 412
           K  + S V E  PV T+HE       S T+SQSKQD EKSSV SS VR+PLEENILL VA
Sbjct: 438 KTPAHSVVSETLPVITNHESSRADTASATSSQSKQDEEKSSVPSSAVRTPLEENILLGVA 497

Query: 413 LEGSKRTLPIEEEMTSSPTPAESQEFAVQRNGSGPPSNKDKKDGPISSFPTTKQNE 468
           LEGSKRTLPIEEEM  SP  AESQEFAVQRNG+GPP+NKDKKDGP SSFP  KQN+
Sbjct: 498 LEGSKRTLPIEEEMNPSPNSAESQEFAVQRNGNGPPANKDKKDGPTSSFPNAKQND 553


>Medtr3g100280.1 | mechanosensitive ion channel-like protein | HC |
           chr3:46094703-46102000 | 20130731
          Length = 716

 Score =  734 bits (1894), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 373/476 (78%), Positives = 395/476 (82%), Gaps = 8/476 (1%)

Query: 1   MGLDFAGKXXXXXXXXXXXXLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 60
           MGLDFAGK            LFMELLGFSTQKW               REIFTNFLSSIM
Sbjct: 241 MGLDFAGKAIYTAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSIM 300

Query: 61  IHATRPFILNEWIQTKIEGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVNVVRNL 120
           IH TRPF+LNEWIQTKIEGYEVSGTVEHVGWWSPTI+RGDDREAVHIPNHKFTVNVVRNL
Sbjct: 301 IHTTRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGDDREAVHIPNHKFTVNVVRNL 360

Query: 121 SQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSL 180
           +QK+HWRIKSYI+ISHLDVNKIN IVADMRKVLAKNPQVEQQ+LHRRVFLE++NPEN +L
Sbjct: 361 TQKSHWRIKSYISISHLDVNKINNIVADMRKVLAKNPQVEQQKLHRRVFLEDINPENTAL 420

Query: 181 MILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYREAESENIPFG 240
            ILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIY E  SENIPFG
Sbjct: 421 KILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYSEPASENIPFG 480

Query: 241 DTIFSRSRAAVNRPFLLIEPQYKVNGEDKVKPSTRSTSGNEEKDAKLDEIVVSDSNGDDN 300
           D+IF+RSRAAVNRPFLLIEP YKVNGEDK KPSTRST GNEEKDAK+DE V SDS  D+N
Sbjct: 481 DSIFTRSRAAVNRPFLLIEPPYKVNGEDKAKPSTRSTRGNEEKDAKVDEPVASDSKSDEN 540

Query: 301 LAATSTSPPGVNSKDKSKSISGVPNQNMGSDSSVEKTSKTMQPKKESAGDVRKGSAVPAS 360
            A TSTSP  VNSKDKSKS S    QNMGSDSSVEKTSKTMQPKKE+AGDV KGS +P  
Sbjct: 541 FAGTSTSPSSVNSKDKSKSKSDAQTQNMGSDSSVEKTSKTMQPKKETAGDVGKGSTIPVP 600

Query: 361 KNLSQSAVPEISPV-TSHE-------SGTASQSKQDGEKSSVTSSPVRSPLEENILLEVA 412
           K  + S V E  PV T+HE       S T+SQSKQD EKSSV SS VR+PLEENILL VA
Sbjct: 601 KTPAHSVVSETLPVITNHESSRADTASATSSQSKQDEEKSSVPSSAVRTPLEENILLGVA 660

Query: 413 LEGSKRTLPIEEEMTSSPTPAESQEFAVQRNGSGPPSNKDKKDGPISSFPTTKQNE 468
           LEGSKRTLPIEEEM  SP  AESQEFAVQRNG+GPP+NKDKKDGP SSFP  KQN+
Sbjct: 661 LEGSKRTLPIEEEMNPSPNSAESQEFAVQRNGNGPPANKDKKDGPTSSFPNAKQND 716


>Medtr5g032090.1 | mechanosensitive ion channel-like protein | HC |
           chr5:13770218-13777530 | 20130731
          Length = 705

 Score =  476 bits (1226), Expect = e-134,   Method: Compositional matrix adjust.
 Identities = 264/482 (54%), Positives = 315/482 (65%), Gaps = 39/482 (8%)

Query: 1   MGLDFAGKXXXXXXXXXXXXLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 60
           MG  FAGK            LFMELLGFSTQKW               REIFTNFLSS M
Sbjct: 229 MGFQFAGKAVYSAVWIAAFSLFMELLGFSTQKWVTAGGFGTVLLTLAGREIFTNFLSSAM 288

Query: 61  IHATRPFILNEWIQTKIEGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVNVVRNL 120
           IHATRPF++NEWIQTKIEGYEVSGTVEHVGWWSPTI+RG+DREAVHIPNHKFTVNVVRNL
Sbjct: 289 IHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHKFTVNVVRNL 348

Query: 121 SQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSL 180
           +QKTHWRIK+++AISHLDVNKIN IVADMRKVLAKNPQVEQQRLHRRVFL+N+NPENQ+L
Sbjct: 349 TQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLDNINPENQAL 408

Query: 181 MILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYREAESENIPFG 240
           MILISCFVKTSHFEEYLCVKE ILLDLLRV+SHHRARLATP+RT+QKIY +A+ +NIP+ 
Sbjct: 409 MILISCFVKTSHFEEYLCVKETILLDLLRVISHHRARLATPVRTLQKIYADADLDNIPYA 468

Query: 241 DTIFSRSRAAV-NRPFLLIEPQYKVNGEDKVKP-STRSTSGNEEK-------DAKLDEIV 291
           DT F+ +   V NRP L+I+  YK N +DK K  STR+T   ++K       D+K ++ V
Sbjct: 469 DTTFNGAGPVVSNRPLLVIDSPYKNNADDKTKSRSTRATVDQDDKTTVRTKLDSKTEDKV 528

Query: 292 ------------VSDSNGDDNLAATSTSPPGVNSKDKS---KSISGVPNQNMGSDSSV-- 334
                         D +  D     S S  G N   KS   K+   VP  +  S+S V  
Sbjct: 529 EPIGAPDTKVRGTQDESEVDAKVMASNSDVGENKPLKSNLNKANMEVPEMSSTSNSKVTG 588

Query: 335 ----EKTSKTMQPKKESAGDVRKGSAVPASKNLSQSAVPEISPVTSHESGTASQSKQDGE 390
                 T K +  K+     V+       S NL  S     S   + ++     + Q GE
Sbjct: 589 LEVDNSTMKDIHVKQSKVQTVKNTKPNVDSDNLVSS-----STNNADKANGNMATNQQGE 643

Query: 391 KSSVTSSPVRSPLEENILLEVALEGSKRTLPIEEEMTSSPTPAESQEFAVQRNGSGPPSN 450
           +     +P R  LEENI+L VAL+GSKRTLPI +E+  + T  E++E A  + G+G P  
Sbjct: 644 RK---PAPPRPVLEENIVLGVALDGSKRTLPINDEI-DTVTTQETKEMAASQGGNGSPKA 699

Query: 451 KD 452
            D
Sbjct: 700 SD 701


>Medtr3g100320.1 | mechanosensitive ion channel-like protein | HC |
           chr3:46127036-46132359 | 20130731
          Length = 591

 Score =  418 bits (1074), Expect = e-117,   Method: Compositional matrix adjust.
 Identities = 211/344 (61%), Positives = 253/344 (73%), Gaps = 10/344 (2%)

Query: 1   MGLDFAGKXXXXXXXXXXXXLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 60
           M +D  GK            LFMELLG S QKW               REIFTNFLSS+M
Sbjct: 232 MSIDLIGKAVYTAVWVAAVTLFMELLGLSAQKWLAAGGMGTVLLSLAGREIFTNFLSSVM 291

Query: 61  IHATRPFILNEWIQTKIEGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVNVVRNL 120
           IHATRPF++NE I+TKI+GYEVSG VEHVGWWSPT+VRG D EAVHIPNHK +VNVVRNL
Sbjct: 292 IHATRPFVVNERIKTKIKGYEVSGRVEHVGWWSPTVVRGADCEAVHIPNHKLSVNVVRNL 351

Query: 121 SQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSL 180
           S+KTHWRIK+++AISHLDVNKI+ I+ADMRKVLAKNP VEQ++LHRRVFLE+VNPENQ+L
Sbjct: 352 SKKTHWRIKTHLAISHLDVNKIDTIIADMRKVLAKNPLVEQKKLHRRVFLEDVNPENQAL 411

Query: 181 MILISCFVKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQKIYREAESENIPFG 240
           MIL+SCFVKTSH EEYL VKEAILLDLLRV+SHH ARLATPIRT+QK+Y +++ E  PF 
Sbjct: 412 MILVSCFVKTSHSEEYLRVKEAILLDLLRVISHHGARLATPIRTIQKMYSDSDLEIDPFD 471

Query: 241 DTIFSRSRAAVNRPFLLIEPQYKVNGEDKVKPSTRSTSGNEEKDAKLDEIVVSDSNGD-D 299
           D IF+RSRA  N PF      Y      K KPST+ST+ N+EKD K+ E +++    + +
Sbjct: 472 DAIFTRSRAKGNHPFPFTNSPY------KAKPSTQSTTVNDEKDVKIRETLITGFKDEGE 525

Query: 300 NLAATSTSPPGVNSKDKSKSISGVPNQNMGSDSS---VEKTSKT 340
            +AAT T    V S+DK KS S    +N+ S+SS   V+K SK+
Sbjct: 526 KIAATWTPSSSVKSQDKLKSTSKAKTRNVASESSDNPVQKASKS 569


>Medtr3g100320.2 | mechanosensitive ion channel-like protein | HC |
           chr3:46126688-46132690 | 20130731
          Length = 430

 Score =  269 bits (687), Expect = 5e-72,   Method: Compositional matrix adjust.
 Identities = 124/182 (68%), Positives = 143/182 (78%)

Query: 1   MGLDFAGKXXXXXXXXXXXXLFMELLGFSTQKWXXXXXXXXXXXXXXXREIFTNFLSSIM 60
           M +D  GK            LFMELLG S QKW               REIFTNFLSS+M
Sbjct: 244 MSIDLIGKAVYTAVWVAAVTLFMELLGLSAQKWLAAGGMGTVLLSLAGREIFTNFLSSVM 303

Query: 61  IHATRPFILNEWIQTKIEGYEVSGTVEHVGWWSPTIVRGDDREAVHIPNHKFTVNVVRNL 120
           IHATRPF++NE I+TKI+GYEVSG VEHVGWWSPT+VRG D EAVHIPNHK +VNVVRNL
Sbjct: 304 IHATRPFVVNERIKTKIKGYEVSGRVEHVGWWSPTVVRGADCEAVHIPNHKLSVNVVRNL 363

Query: 121 SQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSL 180
           S+KTHWRIK+++AISHLDVNKI+ I+ADMRKVLAKNP VEQ++LHRRVFLE+VNPENQ+L
Sbjct: 364 SKKTHWRIKTHLAISHLDVNKIDTIIADMRKVLAKNPLVEQKKLHRRVFLEDVNPENQAL 423

Query: 181 MI 182
           M+
Sbjct: 424 MV 425


>Medtr3g100300.1 | transmembrane protein, putative | LC |
           chr3:46107757-46110672 | 20130731
          Length = 321

 Score = 94.4 bits (233), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 67/160 (41%), Positives = 83/160 (51%), Gaps = 37/160 (23%)

Query: 78  EGYEVSGTV-EHVGWWSPTIVRG---------DDREAVHIPNHKFTVNVVRNLSQKTHWR 127
           E Y++   + E+ GW   TI+R           DREAVHIPNHKF  NVVRN ++   W 
Sbjct: 133 EPYDILAPIKEYYGWGKITIIRDPNYIPNHKFADREAVHIPNHKFADNVVRNPTKI--WN 190

Query: 128 IKSYIAISHLDVNKINIIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQSLMILISCF 187
           ++ Y AI     N INII     + L K PQVEQQ     V L+ ++P  Q+        
Sbjct: 191 LRRYFAIRSF--NDINIIKLIAVRFLLKKPQVEQQT----VVLKAISPGKQTR------- 237

Query: 188 VKTSHFEEYLCVKEAILLDLLRVVSHHRARLATPIRTVQK 227
                       KEAIL DL  VVSHHR  LATPIRTV++
Sbjct: 238 ------------KEAILSDLRSVVSHHRDPLATPIRTVEE 265


>Medtr3g099260.1 | hypothetical protein | LC |
           chr3:45499769-45498191 | 20130731
          Length = 161

 Score = 75.1 bits (183), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 38/93 (40%), Positives = 58/93 (62%), Gaps = 3/93 (3%)

Query: 97  VRGDDREAVHIPNHKFTVNVVRNLSQKTHWRIKSYIAISHLDVNKINIIVADMRKVLAKN 156
           V+  D E V IP H FT N+VRN +   +  I  YI +  L++ K+ II+  M  VL+K+
Sbjct: 72  VQFCDGEIVMIPTHNFTTNIVRNHTHDGYLNI--YIPVERLNITKLKIILTKMCDVLSKS 129

Query: 157 PQVEQQRLHRRVFLENVNPENQSLMILISCFVK 189
             VEQ++ HRRV+ ++V P   ++ ILI C++K
Sbjct: 130 SYVEQEKFHRRVYFDDV-PGKGTVRILIDCYLK 161