Miyakogusa Predicted Gene
- Lj1g3v1514800.2
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v1514800.2 tr|G7J3Z0|G7J3Z0_MEDTR Seryl-tRNA synthetase
OS=Medicago truncatula GN=MTR_3g099470 PE=4
SV=1,84.66,0,TRNASYNTHSER,Serine-tRNA ligase, type1;
tRNA-synt_2b,Aminoacyl-tRNA synthetase, class II (G/ H/ P/
S,CUFF.27429.2
(513 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr3g099470.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC ... 806 0.0
Medtr3g099470.2 | seryl-tRNA synthetase/serine-tRNA ligase | HC ... 626 e-179
Medtr1g082850.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC ... 227 2e-59
Medtr7g029025.1 | seryl-tRNA synthetase/serine-tRNA ligase, puta... 77 3e-14
>Medtr3g099470.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC |
chr3:45589841-45593917 | 20130731
Length = 546
Score = 806 bits (2082), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 397/515 (77%), Positives = 423/515 (82%), Gaps = 4/515 (0%)
Query: 1 MGLHGCLNITTLQSFKLGAFRTYPSSHQRTKAPFLY-SQKPSLPR-PIKALXXXXXXXXX 58
M LHG LN TT Q+ KL + T SS R +P L+ + K PR KAL
Sbjct: 34 MDLHGYLNRTTFQTLKLSSTPTCSSS--RFLSPSLHKTHKFPFPRLTTKALSTSSAAVQT 91
Query: 59 XXXXXXXXXXKGGKPQWKATIDFKWIKDNKEAVAANIRNRNSHANLDLVLHLYDSMFHLQ 118
+GGK QWKATIDFKWIKDNKE VA NI+NRNS ANL+ +LHLYD +F LQ
Sbjct: 92 SPTTTTISDDQGGKHQWKATIDFKWIKDNKETVAVNIKNRNSDANLEFILHLYDKLFILQ 151
Query: 119 KEVERVRGERNAVANKMKGKIEPSERQRLIEEGKKLKEGLIAXXXXXXXXXXXXQQEAQS 178
KEVERVRGERNAVANKMKGK+EPSERQ LIE+GK LKEGL A Q+EAQ
Sbjct: 152 KEVERVRGERNAVANKMKGKLEPSERQTLIEQGKNLKEGLTALEEDLAKLNDELQREAQC 211
Query: 179 IPNITHPDVPIGGEDCSTIIKMVGSAPKFSFPVKDHLQLGKXXXXXXXXXXXEVSGSKFY 238
IPN+THPDVPIGGED STI K VGS+PKFSFPVKDHLQLGK EVSGSKFY
Sbjct: 212 IPNMTHPDVPIGGEDSSTIRKTVGSSPKFSFPVKDHLQLGKELDLFDFDAAAEVSGSKFY 271
Query: 239 YLKNEAVLLEMALLNWTLSEVMKRGFTPITTPEIVRSSVVEKCGFQPRADNTQIYSIEDS 298
YLKNEAVLLEMALLNWTLSEVMKRGFTP+TTPEIVRSSVVEKCGFQPRA NTQ+YSI+DS
Sbjct: 272 YLKNEAVLLEMALLNWTLSEVMKRGFTPLTTPEIVRSSVVEKCGFQPRAKNTQVYSIDDS 331
Query: 299 DQCLIGTAEIPVGGLHMDSILADSLLPLKYVAFSHCFRTEXXXXXXXTRGLYRVHQFSKV 358
DQCLIGTAEIPVGGLHMDSILA+S+LPLKYVAFSHCFRTE TRGLYRVHQFSK+
Sbjct: 332 DQCLIGTAEIPVGGLHMDSILAESMLPLKYVAFSHCFRTEAGAAGTATRGLYRVHQFSKI 391
Query: 359 EMFVFCRPEESEYYHQELIKIEEDIFSSLGLHFKILDMASEDLGAPAYRKSDVEAWMPGL 418
EMF+FCRPEES+YYH+ELIKIEED+FSSLGLHFK LDMASEDLGAPAYRK DVEAWMPGL
Sbjct: 392 EMFIFCRPEESDYYHEELIKIEEDMFSSLGLHFKTLDMASEDLGAPAYRKFDVEAWMPGL 451
Query: 419 ERFGEISSASNCTDYQSRRLGIRYRPSEALAPGPKKSKGNLAPPQFVHTLNATACAVPRM 478
ERFGEISSASNCTDYQSRRLGIRYRPSE L PGPKKSKGNLAPPQFVHTLNATACAVPRM
Sbjct: 452 ERFGEISSASNCTDYQSRRLGIRYRPSEVLVPGPKKSKGNLAPPQFVHTLNATACAVPRM 511
Query: 479 IICLLENYQQEDGSVLIPEPLRPFMGGLNIIARKS 513
IICLLENYQQEDGSVLIPEPLRPFMGGL+II RKS
Sbjct: 512 IICLLENYQQEDGSVLIPEPLRPFMGGLDIITRKS 546
>Medtr3g099470.2 | seryl-tRNA synthetase/serine-tRNA ligase | HC |
chr3:45589841-45593306 | 20130731
Length = 458
Score = 626 bits (1614), Expect = e-179, Method: Compositional matrix adjust.
Identities = 311/425 (73%), Positives = 336/425 (79%), Gaps = 4/425 (0%)
Query: 1 MGLHGCLNITTLQSFKLGAFRTYPSSHQRTKAPFLY-SQKPSLPR-PIKALXXXXXXXXX 58
M LHG LN TT Q+ KL + T SS R +P L+ + K PR KAL
Sbjct: 34 MDLHGYLNRTTFQTLKLSSTPTCSSS--RFLSPSLHKTHKFPFPRLTTKALSTSSAAVQT 91
Query: 59 XXXXXXXXXXKGGKPQWKATIDFKWIKDNKEAVAANIRNRNSHANLDLVLHLYDSMFHLQ 118
+GGK QWKATIDFKWIKDNKE VA NI+NRNS ANL+ +LHLYD +F LQ
Sbjct: 92 SPTTTTISDDQGGKHQWKATIDFKWIKDNKETVAVNIKNRNSDANLEFILHLYDKLFILQ 151
Query: 119 KEVERVRGERNAVANKMKGKIEPSERQRLIEEGKKLKEGLIAXXXXXXXXXXXXQQEAQS 178
KEVERVRGERNAVANKMKGK+EPSERQ LIE+GK LKEGL A Q+EAQ
Sbjct: 152 KEVERVRGERNAVANKMKGKLEPSERQTLIEQGKNLKEGLTALEEDLAKLNDELQREAQC 211
Query: 179 IPNITHPDVPIGGEDCSTIIKMVGSAPKFSFPVKDHLQLGKXXXXXXXXXXXEVSGSKFY 238
IPN+THPDVPIGGED STI K VGS+PKFSFPVKDHLQLGK EVSGSKFY
Sbjct: 212 IPNMTHPDVPIGGEDSSTIRKTVGSSPKFSFPVKDHLQLGKELDLFDFDAAAEVSGSKFY 271
Query: 239 YLKNEAVLLEMALLNWTLSEVMKRGFTPITTPEIVRSSVVEKCGFQPRADNTQIYSIEDS 298
YLKNEAVLLEMALLNWTLSEVMKRGFTP+TTPEIVRSSVVEKCGFQPRA NTQ+YSI+DS
Sbjct: 272 YLKNEAVLLEMALLNWTLSEVMKRGFTPLTTPEIVRSSVVEKCGFQPRAKNTQVYSIDDS 331
Query: 299 DQCLIGTAEIPVGGLHMDSILADSLLPLKYVAFSHCFRTEXXXXXXXTRGLYRVHQFSKV 358
DQCLIGTAEIPVGGLHMDSILA+S+LPLKYVAFSHCFRTE TRGLYRVHQFSK+
Sbjct: 332 DQCLIGTAEIPVGGLHMDSILAESMLPLKYVAFSHCFRTEAGAAGTATRGLYRVHQFSKI 391
Query: 359 EMFVFCRPEESEYYHQELIKIEEDIFSSLGLHFKILDMASEDLGAPAYRKSDVEAWMPGL 418
EMF+FCRPEES+YYH+ELIKIEED+FSSLGLHFK LDMASEDLGAPAYRK DVEAWMPGL
Sbjct: 392 EMFIFCRPEESDYYHEELIKIEEDMFSSLGLHFKTLDMASEDLGAPAYRKFDVEAWMPGL 451
Query: 419 ERFGE 423
ERFGE
Sbjct: 452 ERFGE 456
>Medtr1g082850.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC |
chr1:36859354-36864494 | 20130731
Length = 447
Score = 227 bits (579), Expect = 2e-59, Method: Compositional matrix adjust.
Identities = 135/408 (33%), Positives = 214/408 (52%), Gaps = 19/408 (4%)
Query: 104 LDLVLHLYDSMFHLQKEVERVRGERNAVANKMKGKIE--PSERQRLIEEGKKLKEGLIAX 161
+D V++L Q E E +R E N + NK K++ + I + +++K+ +
Sbjct: 32 VDEVINLDKEWRKRQFEFENLRKEVNKI-NKEVSKLKRGGEDATEFIAKSEEIKKKIAEK 90
Query: 162 XXXXXXXXXXXQQEAQSIPNITHPDVPIGGEDCSTIIKMVGSAPKFSFPVKDHLQLGKXX 221
+ ++I N+ H VPI ++ + ++ + +K+H+ L
Sbjct: 91 EVEAQETLNLLNSKLETIGNLVHDSVPISNDEANNLVVRTWGEKRDEPKLKNHVDLVDLL 150
Query: 222 XXXXXXXXXEVSGSKFYYLKNEAVLLEMALLNWTLSEVMKRGFTPITTPEIVRSSVVEKC 281
+V+G + +YLK + V L AL+ + L+ + +RG+T + TP ++ ++ KC
Sbjct: 151 EIADLKKGADVAGGRGFYLKGDGVFLNQALIMFGLNFLAERGYTALHTPFFIKKDIMSKC 210
Query: 282 GFQPRADNTQIYSI--EDSDQCLIGTAEIPVGGLHMDSILADSLLPLKYVAFSHCFRTEX 339
+ D ++Y + E D+ LI TAE P+ H D + + LPL+Y +S CFR E
Sbjct: 211 AQLAQFDE-ELYKVTGEGDDKYLIATAEQPLCAYHSDEWIHPTRLPLRYAGYSSCFRKEA 269
Query: 340 XXXXXXTRGLYRVHQFSKVEMFVFCRPEESEYY--HQELIKIEEDIFSSLGLHFKILDMA 397
T G++RVHQF KVE F P +++ + H+E++K ED + +L + ++I+ +
Sbjct: 270 GSHGRDTLGIFRVHQFEKVEQFCMTTPNDNDSWDMHEEMLKNSEDFYKALNIPYQIVSIV 329
Query: 398 SEDLGAPAYRKSDVEAWMPGLERFGEISSASNCTDYQSRRLGIRYRPSEALAPGPKKSKG 457
S L A +K D+EAW P + + E+ S SNCTDYQ+R+L IRY G KKS
Sbjct: 330 SGALNDAAAKKYDLEAWFPASKTYRELVSCSNCTDYQARKLEIRY--------GQKKSNE 381
Query: 458 NLAPPQFVHTLNATACAVPRMIICLLENYQQEDGSVLIPEPLRPFMGG 505
L Q+VH LN+T A R I C+LEN Q EDG V IPE LRPFM G
Sbjct: 382 QL--KQYVHLLNSTLTATERTICCILENNQNEDG-VEIPEVLRPFMFG 426
>Medtr7g029025.1 | seryl-tRNA synthetase/serine-tRNA ligase,
putative | LC | chr7:10084902-10086127 | 20130731
Length = 298
Score = 77.4 bits (189), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 51/150 (34%), Positives = 75/150 (50%), Gaps = 33/150 (22%)
Query: 358 VEMFVFCRPEESEYY--HQELIKIEEDIFSSLGLHFKILDMASEDLGAPAYRKSDVEAWM 415
VE F P +++ + H+E++K E + +L + ++I+ + S L + A +K
Sbjct: 123 VEQFCITTPNDNDSWDKHEEMLKKAEHFYEALNIPYQIVSIVSGALNSAAAKKYH----- 177
Query: 416 PGLERFGEISSASNCTDYQSRRLGIRYRPSEALAPGPKKSKGNLAPPQFVHTLNATACAV 475
DYQ+R+L IRY G K L Q+VH LN+T A
Sbjct: 178 ----------------DYQARKLEIRY--------GQTKKSSELLK-QYVHLLNSTLTAT 212
Query: 476 PRMIICLLENYQQEDGSVLIPEPLRPFMGG 505
R I C+LEN Q+EDG V+I + LRPFMGG
Sbjct: 213 QRTICCILENNQKEDG-VVILKVLRPFMGG 241