Miyakogusa Predicted Gene

Lj1g3v1514800.2
Show Alignment: 

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v1514800.2 tr|G7J3Z0|G7J3Z0_MEDTR Seryl-tRNA synthetase
OS=Medicago truncatula GN=MTR_3g099470 PE=4
SV=1,84.66,0,TRNASYNTHSER,Serine-tRNA ligase, type1;
tRNA-synt_2b,Aminoacyl-tRNA synthetase, class II (G/ H/ P/
S,CUFF.27429.2
         (513 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g099470.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC ...   806   0.0  
Medtr3g099470.2 | seryl-tRNA synthetase/serine-tRNA ligase | HC ...   626   e-179
Medtr1g082850.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC ...   227   2e-59
Medtr7g029025.1 | seryl-tRNA synthetase/serine-tRNA ligase, puta...    77   3e-14

>Medtr3g099470.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC |
           chr3:45589841-45593917 | 20130731
          Length = 546

 Score =  806 bits (2082), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 397/515 (77%), Positives = 423/515 (82%), Gaps = 4/515 (0%)

Query: 1   MGLHGCLNITTLQSFKLGAFRTYPSSHQRTKAPFLY-SQKPSLPR-PIKALXXXXXXXXX 58
           M LHG LN TT Q+ KL +  T  SS  R  +P L+ + K   PR   KAL         
Sbjct: 34  MDLHGYLNRTTFQTLKLSSTPTCSSS--RFLSPSLHKTHKFPFPRLTTKALSTSSAAVQT 91

Query: 59  XXXXXXXXXXKGGKPQWKATIDFKWIKDNKEAVAANIRNRNSHANLDLVLHLYDSMFHLQ 118
                     +GGK QWKATIDFKWIKDNKE VA NI+NRNS ANL+ +LHLYD +F LQ
Sbjct: 92  SPTTTTISDDQGGKHQWKATIDFKWIKDNKETVAVNIKNRNSDANLEFILHLYDKLFILQ 151

Query: 119 KEVERVRGERNAVANKMKGKIEPSERQRLIEEGKKLKEGLIAXXXXXXXXXXXXQQEAQS 178
           KEVERVRGERNAVANKMKGK+EPSERQ LIE+GK LKEGL A            Q+EAQ 
Sbjct: 152 KEVERVRGERNAVANKMKGKLEPSERQTLIEQGKNLKEGLTALEEDLAKLNDELQREAQC 211

Query: 179 IPNITHPDVPIGGEDCSTIIKMVGSAPKFSFPVKDHLQLGKXXXXXXXXXXXEVSGSKFY 238
           IPN+THPDVPIGGED STI K VGS+PKFSFPVKDHLQLGK           EVSGSKFY
Sbjct: 212 IPNMTHPDVPIGGEDSSTIRKTVGSSPKFSFPVKDHLQLGKELDLFDFDAAAEVSGSKFY 271

Query: 239 YLKNEAVLLEMALLNWTLSEVMKRGFTPITTPEIVRSSVVEKCGFQPRADNTQIYSIEDS 298
           YLKNEAVLLEMALLNWTLSEVMKRGFTP+TTPEIVRSSVVEKCGFQPRA NTQ+YSI+DS
Sbjct: 272 YLKNEAVLLEMALLNWTLSEVMKRGFTPLTTPEIVRSSVVEKCGFQPRAKNTQVYSIDDS 331

Query: 299 DQCLIGTAEIPVGGLHMDSILADSLLPLKYVAFSHCFRTEXXXXXXXTRGLYRVHQFSKV 358
           DQCLIGTAEIPVGGLHMDSILA+S+LPLKYVAFSHCFRTE       TRGLYRVHQFSK+
Sbjct: 332 DQCLIGTAEIPVGGLHMDSILAESMLPLKYVAFSHCFRTEAGAAGTATRGLYRVHQFSKI 391

Query: 359 EMFVFCRPEESEYYHQELIKIEEDIFSSLGLHFKILDMASEDLGAPAYRKSDVEAWMPGL 418
           EMF+FCRPEES+YYH+ELIKIEED+FSSLGLHFK LDMASEDLGAPAYRK DVEAWMPGL
Sbjct: 392 EMFIFCRPEESDYYHEELIKIEEDMFSSLGLHFKTLDMASEDLGAPAYRKFDVEAWMPGL 451

Query: 419 ERFGEISSASNCTDYQSRRLGIRYRPSEALAPGPKKSKGNLAPPQFVHTLNATACAVPRM 478
           ERFGEISSASNCTDYQSRRLGIRYRPSE L PGPKKSKGNLAPPQFVHTLNATACAVPRM
Sbjct: 452 ERFGEISSASNCTDYQSRRLGIRYRPSEVLVPGPKKSKGNLAPPQFVHTLNATACAVPRM 511

Query: 479 IICLLENYQQEDGSVLIPEPLRPFMGGLNIIARKS 513
           IICLLENYQQEDGSVLIPEPLRPFMGGL+II RKS
Sbjct: 512 IICLLENYQQEDGSVLIPEPLRPFMGGLDIITRKS 546


>Medtr3g099470.2 | seryl-tRNA synthetase/serine-tRNA ligase | HC |
           chr3:45589841-45593306 | 20130731
          Length = 458

 Score =  626 bits (1614), Expect = e-179,   Method: Compositional matrix adjust.
 Identities = 311/425 (73%), Positives = 336/425 (79%), Gaps = 4/425 (0%)

Query: 1   MGLHGCLNITTLQSFKLGAFRTYPSSHQRTKAPFLY-SQKPSLPR-PIKALXXXXXXXXX 58
           M LHG LN TT Q+ KL +  T  SS  R  +P L+ + K   PR   KAL         
Sbjct: 34  MDLHGYLNRTTFQTLKLSSTPTCSSS--RFLSPSLHKTHKFPFPRLTTKALSTSSAAVQT 91

Query: 59  XXXXXXXXXXKGGKPQWKATIDFKWIKDNKEAVAANIRNRNSHANLDLVLHLYDSMFHLQ 118
                     +GGK QWKATIDFKWIKDNKE VA NI+NRNS ANL+ +LHLYD +F LQ
Sbjct: 92  SPTTTTISDDQGGKHQWKATIDFKWIKDNKETVAVNIKNRNSDANLEFILHLYDKLFILQ 151

Query: 119 KEVERVRGERNAVANKMKGKIEPSERQRLIEEGKKLKEGLIAXXXXXXXXXXXXQQEAQS 178
           KEVERVRGERNAVANKMKGK+EPSERQ LIE+GK LKEGL A            Q+EAQ 
Sbjct: 152 KEVERVRGERNAVANKMKGKLEPSERQTLIEQGKNLKEGLTALEEDLAKLNDELQREAQC 211

Query: 179 IPNITHPDVPIGGEDCSTIIKMVGSAPKFSFPVKDHLQLGKXXXXXXXXXXXEVSGSKFY 238
           IPN+THPDVPIGGED STI K VGS+PKFSFPVKDHLQLGK           EVSGSKFY
Sbjct: 212 IPNMTHPDVPIGGEDSSTIRKTVGSSPKFSFPVKDHLQLGKELDLFDFDAAAEVSGSKFY 271

Query: 239 YLKNEAVLLEMALLNWTLSEVMKRGFTPITTPEIVRSSVVEKCGFQPRADNTQIYSIEDS 298
           YLKNEAVLLEMALLNWTLSEVMKRGFTP+TTPEIVRSSVVEKCGFQPRA NTQ+YSI+DS
Sbjct: 272 YLKNEAVLLEMALLNWTLSEVMKRGFTPLTTPEIVRSSVVEKCGFQPRAKNTQVYSIDDS 331

Query: 299 DQCLIGTAEIPVGGLHMDSILADSLLPLKYVAFSHCFRTEXXXXXXXTRGLYRVHQFSKV 358
           DQCLIGTAEIPVGGLHMDSILA+S+LPLKYVAFSHCFRTE       TRGLYRVHQFSK+
Sbjct: 332 DQCLIGTAEIPVGGLHMDSILAESMLPLKYVAFSHCFRTEAGAAGTATRGLYRVHQFSKI 391

Query: 359 EMFVFCRPEESEYYHQELIKIEEDIFSSLGLHFKILDMASEDLGAPAYRKSDVEAWMPGL 418
           EMF+FCRPEES+YYH+ELIKIEED+FSSLGLHFK LDMASEDLGAPAYRK DVEAWMPGL
Sbjct: 392 EMFIFCRPEESDYYHEELIKIEEDMFSSLGLHFKTLDMASEDLGAPAYRKFDVEAWMPGL 451

Query: 419 ERFGE 423
           ERFGE
Sbjct: 452 ERFGE 456


>Medtr1g082850.1 | seryl-tRNA synthetase/serine-tRNA ligase | HC |
           chr1:36859354-36864494 | 20130731
          Length = 447

 Score =  227 bits (579), Expect = 2e-59,   Method: Compositional matrix adjust.
 Identities = 135/408 (33%), Positives = 214/408 (52%), Gaps = 19/408 (4%)

Query: 104 LDLVLHLYDSMFHLQKEVERVRGERNAVANKMKGKIE--PSERQRLIEEGKKLKEGLIAX 161
           +D V++L       Q E E +R E N + NK   K++    +    I + +++K+ +   
Sbjct: 32  VDEVINLDKEWRKRQFEFENLRKEVNKI-NKEVSKLKRGGEDATEFIAKSEEIKKKIAEK 90

Query: 162 XXXXXXXXXXXQQEAQSIPNITHPDVPIGGEDCSTIIKMVGSAPKFSFPVKDHLQLGKXX 221
                        + ++I N+ H  VPI  ++ + ++       +    +K+H+ L    
Sbjct: 91  EVEAQETLNLLNSKLETIGNLVHDSVPISNDEANNLVVRTWGEKRDEPKLKNHVDLVDLL 150

Query: 222 XXXXXXXXXEVSGSKFYYLKNEAVLLEMALLNWTLSEVMKRGFTPITTPEIVRSSVVEKC 281
                    +V+G + +YLK + V L  AL+ + L+ + +RG+T + TP  ++  ++ KC
Sbjct: 151 EIADLKKGADVAGGRGFYLKGDGVFLNQALIMFGLNFLAERGYTALHTPFFIKKDIMSKC 210

Query: 282 GFQPRADNTQIYSI--EDSDQCLIGTAEIPVGGLHMDSILADSLLPLKYVAFSHCFRTEX 339
               + D  ++Y +  E  D+ LI TAE P+   H D  +  + LPL+Y  +S CFR E 
Sbjct: 211 AQLAQFDE-ELYKVTGEGDDKYLIATAEQPLCAYHSDEWIHPTRLPLRYAGYSSCFRKEA 269

Query: 340 XXXXXXTRGLYRVHQFSKVEMFVFCRPEESEYY--HQELIKIEEDIFSSLGLHFKILDMA 397
                 T G++RVHQF KVE F    P +++ +  H+E++K  ED + +L + ++I+ + 
Sbjct: 270 GSHGRDTLGIFRVHQFEKVEQFCMTTPNDNDSWDMHEEMLKNSEDFYKALNIPYQIVSIV 329

Query: 398 SEDLGAPAYRKSDVEAWMPGLERFGEISSASNCTDYQSRRLGIRYRPSEALAPGPKKSKG 457
           S  L   A +K D+EAW P  + + E+ S SNCTDYQ+R+L IRY        G KKS  
Sbjct: 330 SGALNDAAAKKYDLEAWFPASKTYRELVSCSNCTDYQARKLEIRY--------GQKKSNE 381

Query: 458 NLAPPQFVHTLNATACAVPRMIICLLENYQQEDGSVLIPEPLRPFMGG 505
            L   Q+VH LN+T  A  R I C+LEN Q EDG V IPE LRPFM G
Sbjct: 382 QL--KQYVHLLNSTLTATERTICCILENNQNEDG-VEIPEVLRPFMFG 426


>Medtr7g029025.1 | seryl-tRNA synthetase/serine-tRNA ligase,
           putative | LC | chr7:10084902-10086127 | 20130731
          Length = 298

 Score = 77.4 bits (189), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 51/150 (34%), Positives = 75/150 (50%), Gaps = 33/150 (22%)

Query: 358 VEMFVFCRPEESEYY--HQELIKIEEDIFSSLGLHFKILDMASEDLGAPAYRKSDVEAWM 415
           VE F    P +++ +  H+E++K  E  + +L + ++I+ + S  L + A +K       
Sbjct: 123 VEQFCITTPNDNDSWDKHEEMLKKAEHFYEALNIPYQIVSIVSGALNSAAAKKYH----- 177

Query: 416 PGLERFGEISSASNCTDYQSRRLGIRYRPSEALAPGPKKSKGNLAPPQFVHTLNATACAV 475
                           DYQ+R+L IRY        G  K    L   Q+VH LN+T  A 
Sbjct: 178 ----------------DYQARKLEIRY--------GQTKKSSELLK-QYVHLLNSTLTAT 212

Query: 476 PRMIICLLENYQQEDGSVLIPEPLRPFMGG 505
            R I C+LEN Q+EDG V+I + LRPFMGG
Sbjct: 213 QRTICCILENNQKEDG-VVILKVLRPFMGG 241