Miyakogusa Predicted Gene

Lj1g3v1008920.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v1008920.1 Non Characterized Hit- tr|I3SF98|I3SF98_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2 SV=1,96.69,0,MCT-1
PROTEIN,Translation-associated RNA-binding, predicted; seg,NULL; PUA
domain-like,PUA-like doma,CUFF.26672.1
         (181 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g091920.1 | MCT-like PUA RNA-binding-domain protein | HC |...   347   3e-96
Medtr3g091920.2 | MCT-like PUA RNA-binding-domain protein | HC |...   340   4e-94
Medtr4g030200.1 | eukaryotic translation initiation factor SUI1-...    62   4e-10

>Medtr3g091920.1 | MCT-like PUA RNA-binding-domain protein | HC |
           chr3:41941817-41938411 | 20130731
          Length = 181

 Score =  347 bits (890), Expect = 3e-96,   Method: Compositional matrix adjust.
 Identities = 167/181 (92%), Positives = 170/181 (93%)

Query: 1   MFKKFSGEEVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDILPKKSPLIVAKCQNHXXX 60
           MFKKFS EEVSAQNQVKASVQRRIRQSIA+EYPGLEPVLDDILPKKSPLIVAKCQNH   
Sbjct: 1   MFKKFSSEEVSAQNQVKASVQRRIRQSIADEYPGLEPVLDDILPKKSPLIVAKCQNHLNL 60

Query: 61  XXXXXXPLFFSIRDGPYMPTLRLLHQYPDIMKKLQVDRGAIRFVLAGANIMCPGLTSPGG 120
                 PLFFS+RDGPYMPTLRLLHQYPDIMKKLQVDRGAIRFVLAGANIMCPGLTSPGG
Sbjct: 61  VVVNNVPLFFSVRDGPYMPTLRLLHQYPDIMKKLQVDRGAIRFVLAGANIMCPGLTSPGG 120

Query: 121 VLDEEVGAECPVAIMAEGKQHALAIGFTKMSAKDIKAINKGIGVDNLHYLNDGLWKMEQL 180
           VLDEEVGAECPVAIMAEGKQHALAIGFTKMSAKDIKAINKGIGVDNLHYLNDGLWKME+ 
Sbjct: 121 VLDEEVGAECPVAIMAEGKQHALAIGFTKMSAKDIKAINKGIGVDNLHYLNDGLWKMERF 180

Query: 181 D 181
           D
Sbjct: 181 D 181


>Medtr3g091920.2 | MCT-like PUA RNA-binding-domain protein | HC |
           chr3:41941456-41938411 | 20130731
          Length = 198

 Score =  340 bits (873), Expect = 4e-94,   Method: Compositional matrix adjust.
 Identities = 163/178 (91%), Positives = 167/178 (93%)

Query: 4   KFSGEEVSAQNQVKASVQRRIRQSIAEEYPGLEPVLDDILPKKSPLIVAKCQNHXXXXXX 63
           +FS EEVSAQNQVKASVQRRIRQSIA+EYPGLEPVLDDILPKKSPLIVAKCQNH      
Sbjct: 21  RFSSEEVSAQNQVKASVQRRIRQSIADEYPGLEPVLDDILPKKSPLIVAKCQNHLNLVVV 80

Query: 64  XXXPLFFSIRDGPYMPTLRLLHQYPDIMKKLQVDRGAIRFVLAGANIMCPGLTSPGGVLD 123
              PLFFS+RDGPYMPTLRLLHQYPDIMKKLQVDRGAIRFVLAGANIMCPGLTSPGGVLD
Sbjct: 81  NNVPLFFSVRDGPYMPTLRLLHQYPDIMKKLQVDRGAIRFVLAGANIMCPGLTSPGGVLD 140

Query: 124 EEVGAECPVAIMAEGKQHALAIGFTKMSAKDIKAINKGIGVDNLHYLNDGLWKMEQLD 181
           EEVGAECPVAIMAEGKQHALAIGFTKMSAKDIKAINKGIGVDNLHYLNDGLWKME+ D
Sbjct: 141 EEVGAECPVAIMAEGKQHALAIGFTKMSAKDIKAINKGIGVDNLHYLNDGLWKMERFD 198


>Medtr4g030200.1 | eukaryotic translation initiation factor
           SUI1-like protein | HC | chr4:10407181-10415634 |
           20130731
          Length = 600

 Score = 61.6 bits (148), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 50/182 (27%), Positives = 90/182 (49%), Gaps = 9/182 (4%)

Query: 1   MFKKFSGEEVSAQNQVKASVQRRIRQSIAEEYP-GLEPVLDDILPKKSPLIVAKCQNHXX 59
           MFKK    E  +Q ++  + ++++++++ +++P   +  LD +LP K+ + VAK Q    
Sbjct: 1   MFKK--AVEAKSQQRLSGADRKKLKRTVKDKFPRASDSDLDILLPPKTEITVAKFQTRVH 58

Query: 60  XXXXXX-XPLFFSI--RDGPYMPTLRLLHQYPDIMKKLQVDRGAI-RFVLAGANIMCPGL 115
                   P+FF I  R     PT+  L   P+++    +  G + R+V+ GA++M PG+
Sbjct: 59  VYAVEGGFPVFFDIDGRGSDIFPTVYALWMVPELLPAFILKGGEVSRYVIGGADLMFPGI 118

Query: 116 TSPGGVLDEEVGAECPVAIMAEGKQHALAIGFTKM-SAKDIKAINKGIGVDNLHYLNDGL 174
             P   L      E   A+        +A+G T M SA+ +KA  +G  +   H+  D L
Sbjct: 119 LVPPEGLPSFAAGET-WAVKVPENPAPIAVGSTTMSSAEALKAGLRGKALRITHHYRDLL 177

Query: 175 WK 176
           W+
Sbjct: 178 WE 179