Miyakogusa Predicted Gene

Lj1g3v0751200.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v0751200.1 Non Characterized Hit- tr|I1KA37|I1KA37_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.19258 PE,81.01,0,Domain
present in VPS9,Vacuolar sorting protein 9, subgroup; VPS9,Vacuolar
sorting protein 9; SUBFAM,CUFF.26262.1
         (412 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g073360.1 | vacuolar protein sorting-associated protein VP...   555   e-158
Medtr3g009180.1 | vacuolar protein sorting-associated protein VP...   250   2e-66

>Medtr3g073360.1 | vacuolar protein sorting-associated protein VPS9
           | HC | chr3:33082097-33077957 | 20130731
          Length = 478

 Score =  555 bits (1431), Expect = e-158,   Method: Compositional matrix adjust.
 Identities = 290/430 (67%), Positives = 319/430 (74%), Gaps = 54/430 (12%)

Query: 1   MEAAFKAHPLWAGCXXXXXXXXXXXXXKYVMTKLFSRVFASLPNDVKFDDQISEKMGLIQ 60
           MEAAFKAHPLWAGC             KYVMTKLF RVFAS+P+DVK D+Q+SEKM LIQ
Sbjct: 57  MEAAFKAHPLWAGCSDDELESAGEGLEKYVMTKLFPRVFASVPDDVKLDEQLSEKMALIQ 116

Query: 61  QFIRPENLDIMPAFQNETSWLLAQKELQKINMYKAPRDKLICILNCCKVIGNLLLNASLA 120
           QFIRPENLDI P FQNETSWLLAQKELQKINMYKAPRDKL CILNCCKVIGNLLLNASLA
Sbjct: 117 QFIRPENLDIKPPFQNETSWLLAQKELQKINMYKAPRDKLTCILNCCKVIGNLLLNASLA 176

Query: 121 SKENPPGADEFLPVLIYVTIKANPPQLHSNLQYIQRFRRQTRLAGEAAYYFTNMLSAESF 180
           SK+NPPGADEFLPVLIYVT+KANPPQLHSNL YIQRFRRQ+RL GEA+YYFTNMLSAESF
Sbjct: 177 SKDNPPGADEFLPVLIYVTLKANPPQLHSNLLYIQRFRRQSRLVGEASYYFTNMLSAESF 236

Query: 181 ISNIDAKAISMDETEFERNMEFSRALLSGLSVDTQDPNSPYQNYGQHPRAEPAKHRNKAL 240
           ISNIDA AISMDETEFERNMEF+RALLSGLSVDTQDPNSPYQN+ QH        +NKA 
Sbjct: 237 ISNIDANAISMDETEFERNMEFARALLSGLSVDTQDPNSPYQNHVQH-------DKNKA- 288

Query: 241 NDNKDPTLRTPSSVAKSESKRVSFSDELLITKVPSLSDLENKGASMILKEDKLNEIFGEF 300
                     PSS AK ESK+V+F+ E  I KVPSLSDLENKGA MI+KEDKLN++F EF
Sbjct: 289 ----------PSSDAKLESKKVTFASESSIAKVPSLSDLENKGACMIIKEDKLNDVFREF 338

Query: 301 PYLFASVGDLTVGDVEDLLNNYKQLVFKYVSLSKGLGVXXXXXXXXXXXD---------- 350
           PY+FASVGDLTV DVEDLL NYK+LV KYV LSKGLGV           +          
Sbjct: 339 PYMFASVGDLTVSDVEDLLKNYKRLVSKYVCLSKGLGVSSSSHHPSNPQNNAQQDHVETL 398

Query: 351 ----------------------HVESETTLDSSEGESVGAIDEPEKSIDTMEDSSEKVSP 388
                                 HVESETT+DSS+   V + ++ EKS+DT ED+S+    
Sbjct: 399 SKGLASSSSSHHPSNPQNNAQGHVESETTIDSSDNGPVASNEKSEKSLDTSEDNSDP--- 455

Query: 389 LEEAKSESDL 398
            EE KS+S L
Sbjct: 456 -EEKKSDSGL 464


>Medtr3g009180.1 | vacuolar protein sorting-associated protein VPS9
           | HC | chr3:1829947-1834344 | 20130731
          Length = 391

 Score =  250 bits (638), Expect = 2e-66,   Method: Compositional matrix adjust.
 Identities = 115/204 (56%), Positives = 154/204 (75%), Gaps = 1/204 (0%)

Query: 1   MEAAFKAHPLWAGCXXXXXXXXXXXXXKYVMTKLFSRVFASLPNDVKFDDQISEKMGLIQ 60
           ME A + HPLWA               KY+MTKLFSR FA+ P D K D +ISEK+ L+Q
Sbjct: 60  MEVAIRNHPLWATATEEDIDCAMEGLEKYIMTKLFSRTFAASPEDAKIDHEISEKISLLQ 119

Query: 61  QFIRPENLDIMPAFQNETSWLLAQKELQKINMYKAPRDKLICILNCCKVIGNLLLNASLA 120
            F++PE+LDI P   NE SWLLA+KELQKIN +KAP++KL  I+NCC+VI NLLLNA++ 
Sbjct: 120 TFLKPEHLDIPPVLHNEASWLLAEKELQKINAFKAPQEKLSTIMNCCRVINNLLLNAAM- 178

Query: 121 SKENPPGADEFLPVLIYVTIKANPPQLHSNLQYIQRFRRQTRLAGEAAYYFTNMLSAESF 180
           S+  P GAD+F+PVLIYVTIKANPP LHSNL++I+ +RRQT+L  EA YYFTN++SA++F
Sbjct: 179 SEYVPAGADDFIPVLIYVTIKANPPMLHSNLKFIKLYRRQTKLISEAEYYFTNLVSAKTF 238

Query: 181 ISNIDAKAISMDETEFERNMEFSR 204
           I  +++K++S+DE +FE  M+ ++
Sbjct: 239 IIELNSKSLSIDEIKFEECMQAAK 262