Miyakogusa Predicted Gene

Lj1g3v0130120.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj1g3v0130120.1 Non Characterized Hit- tr|I1LFY1|I1LFY1_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.48450
PE,92.16,0,seg,NULL; Peptidase_M16_C,Peptidase M16, C-terminal;
Peptidase_M16,Peptidase M16, N-terminal; PITRIL,CUFF.25269.1
         (1020 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr5g007380.2 | insulinase (peptidase family M16) | HC | chr5:...  1895   0.0  
Medtr5g007380.1 | insulinase (peptidase family M16) | HC | chr5:...  1895   0.0  
Medtr8g101950.1 | peptidase M16 inactive domain protein | HC | c...    60   1e-08

>Medtr5g007380.2 | insulinase (peptidase family M16) | HC |
            chr5:1336812-1323954 | 20130731
          Length = 1258

 Score = 1895 bits (4908), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 916/1021 (89%), Positives = 955/1021 (93%), Gaps = 1/1021 (0%)

Query: 1    MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPTSTKDDDDLLPCVLDALNEIA 60
            MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPT+TKD DDLLP VLDALNEIA
Sbjct: 238  MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPTTTKDSDDLLPSVLDALNEIA 297

Query: 61   FHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKW 120
            FHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKW
Sbjct: 298  FHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKW 357

Query: 121  DADKIRKFHECWYFPANATLYIVGDVDNISKTVYQIEAVFGQTGLDNEKGSVATPSAFGA 180
            DA+KIRKFHE WYFPANATLYIVGD+DNI KTV QIEAVFGQTG+DNEKGS ATPSAFGA
Sbjct: 358  DAEKIRKFHERWYFPANATLYIVGDIDNIPKTVGQIEAVFGQTGVDNEKGSGATPSAFGA 417

Query: 181  MASFLVPKLSVGLGGNSIERSANTMDQSKISKKERHAVRPPVKHNWYLPGSSTNLKPPQI 240
            MASFLVPKLSVGLGGNSIERS NT+DQSK+  KER  VRPPV HNW LPGSS NL PPQI
Sbjct: 418  MASFLVPKLSVGLGGNSIERSTNTLDQSKVFNKERQVVRPPVTHNWSLPGSSANLNPPQI 477

Query: 241  FQHELLQNFSINMFCKIPVNKVRTYRDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSVE 300
            FQHELLQNFSINMFCKIPVNKV+TYRDLR VLMKRIFLSALHFRINTRYKSSNPPFTSVE
Sbjct: 478  FQHELLQNFSINMFCKIPVNKVQTYRDLRIVLMKRIFLSALHFRINTRYKSSNPPFTSVE 537

Query: 301  LDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLKDS 360
            LDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLKDS
Sbjct: 538  LDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLKDS 597

Query: 361  EHLAAMIDNVSSVDNLDFIMESDALGHKVMDQRQGHESLLSLAGTVTLDEVNSVGAKVLE 420
            EHLAAMIDNVSSVDNLDFIMESDAL HKVMDQRQGHESLL++AGTVTLDEVNSVGA+VLE
Sbjct: 598  EHLAAMIDNVSSVDNLDFIMESDALSHKVMDQRQGHESLLAVAGTVTLDEVNSVGAEVLE 657

Query: 421  FIADFGXXXXXXXXXXXXXXXXXFHIEGAGETEFKISSTEITDAIKAGLDYPIQPEPELE 480
            FIADFG                  HIEG GETEFKISSTEITDAIKAGLD PI+PEPELE
Sbjct: 658  FIADFGKPTAPLPAAIVACVPKKVHIEGVGETEFKISSTEITDAIKAGLDDPIEPEPELE 717

Query: 481  VPKELIPSSKLEELKEQRNPTFIPVSPETNATKLHDKETGITRRRLANGIPVNYKISKSE 540
            VPKEL+PSS L+ELKEQR PTFIPVSPET+ATKLHD+ETGITRRRLANGIPVNYKISKSE
Sbjct: 718  VPKELVPSSNLQELKEQRKPTFIPVSPETDATKLHDEETGITRRRLANGIPVNYKISKSE 777

Query: 541  TQSGVMRLIVGGGRAAETPESRGSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCSLE 600
            TQSGVMRLIVGGGRAAE+ +S+GSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCSLE
Sbjct: 778  TQSGVMRLIVGGGRAAESSDSKGSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCSLE 837

Query: 601  STEEFISMEFRFTLRDNGMRAAFQLLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLER 660
            STEEFISMEFRFTLRDNGMRAAFQLLHMVLE+SVWLDDAFDRARQLYLSYYRSIPKSLER
Sbjct: 838  STEEFISMEFRFTLRDNGMRAAFQLLHMVLEHSVWLDDAFDRARQLYLSYYRSIPKSLER 897

Query: 661  STAHKLMVAMLDGDERFIEPTPKSLENLTLQTVKDAVMNQFVGDNMEVSIVGDFTEEEIE 720
            STAHKLMVAMLDGDERF EPTP SLENLTLQ+VKDAVMNQFVGDNMEVSIVGDFTEE+IE
Sbjct: 898  STAHKLMVAMLDGDERFTEPTPSSLENLTLQSVKDAVMNQFVGDNMEVSIVGDFTEEDIE 957

Query: 721  SCILDYLGTAQATRNFKREQEFYPPSFRSSPSDLLSQEVFLKDTDERACAYIAGPAPNRW 780
            SCILDYLGTAQATRNFK EQEF PPSFRSS S L  QEVFL DTDERACAYIAGPAPNRW
Sbjct: 958  SCILDYLGTAQATRNFKSEQEFIPPSFRSSSSGLQFQEVFLNDTDERACAYIAGPAPNRW 1017

Query: 781  GFTVDGKDLLESINNAATINDDGAKSDA-PQAGGLQRSLRGHPLFFGITMGLLSEIINSR 839
            GFTVDGKDLLE+I+NA+++ND+G KSDA P  GGLQ+SLR HPLFFGITMGLLSEIINSR
Sbjct: 1018 GFTVDGKDLLETIDNASSVNDNGTKSDAVPTEGGLQKSLRNHPLFFGITMGLLSEIINSR 1077

Query: 840  LFTNVRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGLHSNKI 899
            LFT VRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGLHSN+I
Sbjct: 1078 LFTTVRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGLHSNRI 1137

Query: 900  TDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLTFLYDAATIED 959
            TDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLT LY+AAT+ED
Sbjct: 1138 TDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLTSLYEAATVED 1197

Query: 960  IYRAYEQLKVDENSLYSCIGIAGAEAVQDIAAPLVEEEPIDTYPGVIPVGRGLSTMTRPT 1019
             Y AYEQLKVDE+SLYSCIG+AGA+  Q+I  P+ EEE  + YPGV+P+GRGLSTMTRPT
Sbjct: 1198 TYLAYEQLKVDEDSLYSCIGVAGAQTAQNIEVPIEEEEAGEGYPGVLPMGRGLSTMTRPT 1257

Query: 1020 T 1020
            T
Sbjct: 1258 T 1258


>Medtr5g007380.1 | insulinase (peptidase family M16) | HC |
            chr5:1336812-1323954 | 20130731
          Length = 1258

 Score = 1895 bits (4908), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 916/1021 (89%), Positives = 955/1021 (93%), Gaps = 1/1021 (0%)

Query: 1    MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPTSTKDDDDLLPCVLDALNEIA 60
            MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPT+TKD DDLLP VLDALNEIA
Sbjct: 238  MIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHAPTTTKDSDDLLPSVLDALNEIA 297

Query: 61   FHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKW 120
            FHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKW
Sbjct: 298  FHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFPIGLEEQIKKW 357

Query: 121  DADKIRKFHECWYFPANATLYIVGDVDNISKTVYQIEAVFGQTGLDNEKGSVATPSAFGA 180
            DA+KIRKFHE WYFPANATLYIVGD+DNI KTV QIEAVFGQTG+DNEKGS ATPSAFGA
Sbjct: 358  DAEKIRKFHERWYFPANATLYIVGDIDNIPKTVGQIEAVFGQTGVDNEKGSGATPSAFGA 417

Query: 181  MASFLVPKLSVGLGGNSIERSANTMDQSKISKKERHAVRPPVKHNWYLPGSSTNLKPPQI 240
            MASFLVPKLSVGLGGNSIERS NT+DQSK+  KER  VRPPV HNW LPGSS NL PPQI
Sbjct: 418  MASFLVPKLSVGLGGNSIERSTNTLDQSKVFNKERQVVRPPVTHNWSLPGSSANLNPPQI 477

Query: 241  FQHELLQNFSINMFCKIPVNKVRTYRDLRNVLMKRIFLSALHFRINTRYKSSNPPFTSVE 300
            FQHELLQNFSINMFCKIPVNKV+TYRDLR VLMKRIFLSALHFRINTRYKSSNPPFTSVE
Sbjct: 478  FQHELLQNFSINMFCKIPVNKVQTYRDLRIVLMKRIFLSALHFRINTRYKSSNPPFTSVE 537

Query: 301  LDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLKDS 360
            LDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLKDS
Sbjct: 538  LDHSDSGREGCTVTTLTITAEPKNWQNAIRVAVHEVRRLKEFGVTQGELTRYLDALLKDS 597

Query: 361  EHLAAMIDNVSSVDNLDFIMESDALGHKVMDQRQGHESLLSLAGTVTLDEVNSVGAKVLE 420
            EHLAAMIDNVSSVDNLDFIMESDAL HKVMDQRQGHESLL++AGTVTLDEVNSVGA+VLE
Sbjct: 598  EHLAAMIDNVSSVDNLDFIMESDALSHKVMDQRQGHESLLAVAGTVTLDEVNSVGAEVLE 657

Query: 421  FIADFGXXXXXXXXXXXXXXXXXFHIEGAGETEFKISSTEITDAIKAGLDYPIQPEPELE 480
            FIADFG                  HIEG GETEFKISSTEITDAIKAGLD PI+PEPELE
Sbjct: 658  FIADFGKPTAPLPAAIVACVPKKVHIEGVGETEFKISSTEITDAIKAGLDDPIEPEPELE 717

Query: 481  VPKELIPSSKLEELKEQRNPTFIPVSPETNATKLHDKETGITRRRLANGIPVNYKISKSE 540
            VPKEL+PSS L+ELKEQR PTFIPVSPET+ATKLHD+ETGITRRRLANGIPVNYKISKSE
Sbjct: 718  VPKELVPSSNLQELKEQRKPTFIPVSPETDATKLHDEETGITRRRLANGIPVNYKISKSE 777

Query: 541  TQSGVMRLIVGGGRAAETPESRGSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCSLE 600
            TQSGVMRLIVGGGRAAE+ +S+GSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCSLE
Sbjct: 778  TQSGVMRLIVGGGRAAESSDSKGSVIVGVRTLSEGGRVGNFSREQVELFCVNHLINCSLE 837

Query: 601  STEEFISMEFRFTLRDNGMRAAFQLLHMVLENSVWLDDAFDRARQLYLSYYRSIPKSLER 660
            STEEFISMEFRFTLRDNGMRAAFQLLHMVLE+SVWLDDAFDRARQLYLSYYRSIPKSLER
Sbjct: 838  STEEFISMEFRFTLRDNGMRAAFQLLHMVLEHSVWLDDAFDRARQLYLSYYRSIPKSLER 897

Query: 661  STAHKLMVAMLDGDERFIEPTPKSLENLTLQTVKDAVMNQFVGDNMEVSIVGDFTEEEIE 720
            STAHKLMVAMLDGDERF EPTP SLENLTLQ+VKDAVMNQFVGDNMEVSIVGDFTEE+IE
Sbjct: 898  STAHKLMVAMLDGDERFTEPTPSSLENLTLQSVKDAVMNQFVGDNMEVSIVGDFTEEDIE 957

Query: 721  SCILDYLGTAQATRNFKREQEFYPPSFRSSPSDLLSQEVFLKDTDERACAYIAGPAPNRW 780
            SCILDYLGTAQATRNFK EQEF PPSFRSS S L  QEVFL DTDERACAYIAGPAPNRW
Sbjct: 958  SCILDYLGTAQATRNFKSEQEFIPPSFRSSSSGLQFQEVFLNDTDERACAYIAGPAPNRW 1017

Query: 781  GFTVDGKDLLESINNAATINDDGAKSDA-PQAGGLQRSLRGHPLFFGITMGLLSEIINSR 839
            GFTVDGKDLLE+I+NA+++ND+G KSDA P  GGLQ+SLR HPLFFGITMGLLSEIINSR
Sbjct: 1018 GFTVDGKDLLETIDNASSVNDNGTKSDAVPTEGGLQKSLRNHPLFFGITMGLLSEIINSR 1077

Query: 840  LFTNVRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGLHSNKI 899
            LFT VRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGLHSN+I
Sbjct: 1078 LFTTVRDSLGLTYDVSFELNLFDRLKLGWYVISVTSTPSKVHKAVDACKNVLRGLHSNRI 1137

Query: 900  TDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLTFLYDAATIED 959
            TDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLT LY+AAT+ED
Sbjct: 1138 TDRELDRAKRTLLMRHEAEIKSNAYWLGLLAHLQASSVPRKDISCIKDLTSLYEAATVED 1197

Query: 960  IYRAYEQLKVDENSLYSCIGIAGAEAVQDIAAPLVEEEPIDTYPGVIPVGRGLSTMTRPT 1019
             Y AYEQLKVDE+SLYSCIG+AGA+  Q+I  P+ EEE  + YPGV+P+GRGLSTMTRPT
Sbjct: 1198 TYLAYEQLKVDEDSLYSCIGVAGAQTAQNIEVPIEEEEAGEGYPGVLPMGRGLSTMTRPT 1257

Query: 1020 T 1020
            T
Sbjct: 1258 T 1258


>Medtr8g101950.1 | peptidase M16 inactive domain protein | HC |
           chr8:42848743-42859442 | 20130731
          Length = 978

 Score = 60.5 bits (145), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 49/172 (28%), Positives = 77/172 (44%), Gaps = 19/172 (11%)

Query: 1   MIEHVAFLGSKKRE-----KLLGT-----GARSNAYTDFHHTVFHIHAPTSTKDDDDLLP 50
           ++EH+AF  +K+       K L +     GA  NA T    TV+ +  P    D  +LL 
Sbjct: 85  IVEHLAFSATKRYNNHDIVKFLESIGAEFGACQNAVTSSDDTVYELLVPV---DKPELLS 141

Query: 51  CVLDALNEIAFHPKFLSSRIEKERRAILSELQMMNTIEYRVDCQLLQHLHSENKLSKRFP 110
             +  L E +   +     +EKER A++ E +       R+       L   +K ++R P
Sbjct: 142 QAISILAEFSSEIRVSKDDLEKERGAVMEEYRGSRNATGRLQDAHWTLLMEGSKYAERLP 201

Query: 111 IGLEEQIKKWDADKIRKFHECWYFPANATLYIVGDVDNISKTVYQIEAVFGQ 162
           IGLE+ I+    + +R F++ WY   N  +  VG V      V  I+  FGQ
Sbjct: 202 IGLEKVIRTVSPETVRHFYKKWYHLCNMAVIAVGSV------VELIKVHFGQ 247