Miyakogusa Predicted Gene
- Lj1g3v0047810.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj1g3v0047810.1 Non Characterized Hit- tr|I1JB03|I1JB03_SOYBN
Uncharacterized protein OS=Glycine max PE=4 SV=1,74.06,0,Pseudouridine
synthase,Pseudouridine synthase, catalytic domain;
PseudoU_synth_2,Pseudouridine
synth,NODE_53752_length_1380_cov_34.390579.path2.1
(429 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr5g004910.1 | RNA pseudouridine synthase | HC | chr5:186083-... 561 e-160
Medtr5g004910.2 | RNA pseudouridine synthase | HC | chr5:186052-... 508 e-144
Medtr7g029385.1 | RNA pseudouridine synthase | HC | chr7:1045307... 277 2e-74
Medtr3g114540.1 | RNA pseudouridine synthase, putative | HC | ch... 63 5e-10
Medtr1g095580.1 | pseudouridine synthase Rlu family protein | HC... 60 4e-09
Medtr1g095580.2 | pseudouridine synthase Rlu family protein | HC... 60 4e-09
Medtr1g095580.3 | pseudouridine synthase Rlu family protein | HC... 60 5e-09
Medtr3g098940.2 | pseudouridine synthase Rlu family protein | HC... 56 8e-08
Medtr3g098940.1 | pseudouridine synthase Rlu family protein | HC... 55 1e-07
>Medtr5g004910.1 | RNA pseudouridine synthase | HC |
chr5:186083-183651 | 20130731
Length = 430
Score = 561 bits (1447), Expect = e-160, Method: Compositional matrix adjust.
Identities = 296/434 (68%), Positives = 341/434 (78%), Gaps = 15/434 (3%)
Query: 6 IVLRVGV-LRSWRSRHFSATSEQDAKAKWHTLPPVTATVKGQGNQVSSSTSTTALKWVLR 64
+ +RVG +RSW R ++S KW TLPPV+ TV SSSTST ALKWV+R
Sbjct: 1 MYVRVGAAVRSWSRRRQLSSSAVAEDGKWLTLPPVSTTVN----TSSSSTSTPALKWVIR 56
Query: 65 CCPNLPRTLVHKLFRLRQVRVAIQ---DNRLKRVTAKDTLNSGDCIVLPHSVKE----IP 117
CCP LP+TL+HKLFRL+QVR+ + DN+ KRVT KDTLNSGD I LP+SVK+ P
Sbjct: 57 CCPELPKTLIHKLFRLKQVRMLPRQQDDNKFKRVTPKDTLNSGDRIFLPNSVKQQIPLTP 116
Query: 118 IPKQQQLECHTTAKEISFIRSLVIYKDPAILVLNKPPGMPVQGGIDIKRSLDVVAASCLK 177
P + + TAK+I+FIRSLVIYKDP ILVLNKPPGMPVQGGI+IK SLD VAA L
Sbjct: 117 TPPKHESPLTLTAKQINFIRSLVIYKDPHILVLNKPPGMPVQGGINIKLSLDAVAAQSLN 176
Query: 178 YEYSEPPRLVHRLDRDCSGILVMGRTQTSATVLHSIFREKTSSASDDLG-TENRVLQRKY 236
Y+YS+PPRLVHRLDRDCSGILVMGRT+TS +LHSIFREKTS AS++ TE +LQR+Y
Sbjct: 177 YDYSQPPRLVHRLDRDCSGILVMGRTKTSTALLHSIFREKTSRASNEENDTEKIILQRRY 236
Query: 237 RALVIGCPRRPRGLVTASLGKVVVDNGKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGY 296
ALV+GCPRR G+V A LGKVVVDNGKSDRITIVDNS+LLSSQHA+TEYRVI SSSHG+
Sbjct: 237 WALVLGCPRRSGGVVNAPLGKVVVDNGKSDRITIVDNSALLSSQHAVTEYRVISSSSHGF 296
Query: 297 TWLELSPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRKWSHFDXXXXXXXXXXXXXXX 356
TWLELSPLTGRKHQLRVHCAEVLGTPIVGDYKYG QAH+KW FD
Sbjct: 297 TWLELSPLTGRKHQLRVHCAEVLGTPIVGDYKYGRQAHKKWGQFDFSNLEDSTEEHLKEE 356
Query: 357 TLPFGLNLNKGSINEKQPRLHLHCKQIVLPDISQALKHVQ--SDSSYDLSQVKALELVAA 414
LPFGLNLNKGSI++K PRLHLHCKQIVLP+ISQAL++VQ SDSSYDLS V++LEL A
Sbjct: 357 ALPFGLNLNKGSISDKHPRLHLHCKQIVLPNISQALQNVQSVSDSSYDLSAVESLELEAD 416
Query: 415 MPPYMRRSWDVMNS 428
+PPYM++SW+V NS
Sbjct: 417 LPPYMKKSWEVTNS 430
>Medtr5g004910.2 | RNA pseudouridine synthase | HC |
chr5:186052-183651 | 20130731
Length = 390
Score = 508 bits (1309), Expect = e-144, Method: Compositional matrix adjust.
Identities = 269/431 (62%), Positives = 307/431 (71%), Gaps = 49/431 (11%)
Query: 6 IVLRVGV-LRSWRSRHFSATSEQDAKAKWHTLPPVTATVKGQGNQVSSSTSTTALKWVLR 64
+ +RVG +RSW R ++S KW TLPPV
Sbjct: 1 MYVRVGAAVRSWSRRRQLSSSAVAEDGKWLTLPPVRM----------------------- 37
Query: 65 CCPNLPRTLVHKLFRLRQVRVAIQDNRLKRVTAKDTLNSGDCIVLPHSVKE----IPIPK 120
LPR DN+ KRVT KDTLNSGD I LP+SVK+ P P
Sbjct: 38 ----LPRQQ--------------DDNKFKRVTPKDTLNSGDRIFLPNSVKQQIPLTPTPP 79
Query: 121 QQQLECHTTAKEISFIRSLVIYKDPAILVLNKPPGMPVQGGIDIKRSLDVVAASCLKYEY 180
+ + TAK+I+FIRSLVIYKDP ILVLNKPPGMPVQGGI+IK SLD VAA L Y+Y
Sbjct: 80 KHESPLTLTAKQINFIRSLVIYKDPHILVLNKPPGMPVQGGINIKLSLDAVAAQSLNYDY 139
Query: 181 SEPPRLVHRLDRDCSGILVMGRTQTSATVLHSIFREKTSSASDDLG-TENRVLQRKYRAL 239
S+PPRLVHRLDRDCSGILVMGRT+TS +LHSIFREKTS AS++ TE +LQR+Y AL
Sbjct: 140 SQPPRLVHRLDRDCSGILVMGRTKTSTALLHSIFREKTSRASNEENDTEKIILQRRYWAL 199
Query: 240 VIGCPRRPRGLVTASLGKVVVDNGKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGYTWL 299
V+GCPRR G+V A LGKVVVDNGKSDRITIVDNS+LLSSQHA+TEYRVI SSSHG+TWL
Sbjct: 200 VLGCPRRSGGVVNAPLGKVVVDNGKSDRITIVDNSALLSSQHAVTEYRVISSSSHGFTWL 259
Query: 300 ELSPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRKWSHFDXXXXXXXXXXXXXXXTLP 359
ELSPLTGRKHQLRVHCAEVLGTPIVGDYKYG QAH+KW FD LP
Sbjct: 260 ELSPLTGRKHQLRVHCAEVLGTPIVGDYKYGRQAHKKWGQFDFSNLEDSTEEHLKEEALP 319
Query: 360 FGLNLNKGSINEKQPRLHLHCKQIVLPDISQALKHVQ--SDSSYDLSQVKALELVAAMPP 417
FGLNLNKGSI++K PRLHLHCKQIVLP+ISQAL++VQ SDSSYDLS V++LEL A +PP
Sbjct: 320 FGLNLNKGSISDKHPRLHLHCKQIVLPNISQALQNVQSVSDSSYDLSAVESLELEADLPP 379
Query: 418 YMRRSWDVMNS 428
YM++SW+V NS
Sbjct: 380 YMKKSWEVTNS 390
>Medtr7g029385.1 | RNA pseudouridine synthase | HC |
chr7:10453073-10460793 | 20130731
Length = 534
Score = 277 bits (708), Expect = 2e-74, Method: Compositional matrix adjust.
Identities = 161/421 (38%), Positives = 241/421 (57%), Gaps = 30/421 (7%)
Query: 35 TLPPVTATVKGQGNQVSSSTS---TTALKWVLRCCPNLPRTLVHKLFRLRQVRVA----- 86
+LPP + N S+ TA+ W+ +P +++ FR V++
Sbjct: 114 SLPPFPSHPLPGKNSTSAPGERDRVTAVNWIKYYFKGVPSSVIESHFREGLVQMEDLIAG 173
Query: 87 ---IQDNRL----KRVTAKDTLNSGDCIVLPHSVKEIPIPKQQQL----ECHTTAKEISF 135
IQ+ L +++ D + G + +P S+ E I ++ + A EI +
Sbjct: 174 DSFIQNKELLKPMRKIKPNDVMKPGSRVHVPVSIAETRISRRYDAIPSGTLYPNADEIKY 233
Query: 136 IRSLVIYKDPAILVLNKPPGMPVQGGIDIKRSLDVVAASCLKYEYSEPPRLVHRLDRDCS 195
++ LV+YKD AI+VLNKPP +PV+G + + S+D +AA+ L Y+Y E P+LVHRLDR+ S
Sbjct: 234 LQRLVMYKDFAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRETS 293
Query: 196 GILVMGRTQTSATVLHSIFREKTSSASDDLGTENRVLQ---RKYRALVIGCPRRPRGLVT 252
GIL+MGRT+ S + L +F T++A N + ++Y ALVIG P+ G++
Sbjct: 294 GILLMGRTKDSVSHLQWLF-SNTNNAKSSCKAWNDACEATHQRYWALVIGTPKEKEGVIH 352
Query: 253 ASLGKVVVDNGKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGYTWLELSPLTGRKHQLR 312
A L KV++++ K++R+ + +SS+ Q A+TEYRV+G +G +W+EL PLT RKHQLR
Sbjct: 353 APLSKVLMNDRKTERVMLAHHSSIEPHQEAVTEYRVLGPMINGCSWIELRPLTYRKHQLR 412
Query: 313 VHCAEVLGTPIVGDYKYGWQAHRKWSHFDXXXXXXXXXXXXXXXTLPFGLNLNKGSINEK 372
VHCAE LGTPIVGDYKYGW H +W P GL++ KGS+ K
Sbjct: 413 VHCAEALGTPIVGDYKYGWLVHSRWKQMARVDMEPTTGKPYKLRR-PEGLDVQKGSVLSK 471
Query: 373 QPRLHLHCKQIVLPDISQALKHVQSDSSYDLS-----QVKALELVAAMPPYMRRSWDVMN 427
P LHL+C+++VLP+I++ L HV SS +L Q L VA MP +MR SW++M+
Sbjct: 472 VPLLHLNCRELVLPNIAKFL-HVLGKSSEELHPSLSLQPDILRFVATMPNHMRISWNLMS 530
Query: 428 S 428
S
Sbjct: 531 S 531
>Medtr3g114540.1 | RNA pseudouridine synthase, putative | HC |
chr3:53482122-53487173 | 20130731
Length = 456
Score = 63.2 bits (152), Expect = 5e-10, Method: Compositional matrix adjust.
Identities = 48/151 (31%), Positives = 77/151 (50%), Gaps = 23/151 (15%)
Query: 184 PRLVHRLDRDCSGILVMGRTQTSATVLHSIFREKTSSASDDLGTENRVLQRKYRALVIGC 243
P +VHRLD+ SG+LV+ + + S L F+ +T ++R Y +L G
Sbjct: 238 PGIVHRLDKGTSGLLVVAKNEHSHMKLSEQFKLRT-------------IKRVYVSLTAGV 284
Query: 244 PRRPRGLVTASLGKVVVDNGKSDRITIVDNSSLLSS---QHAITEYRVIGSSSHGYTWL- 299
P G V +G+ ++R+ ++ + L++S +HA + Y+VI + G L
Sbjct: 285 PTPVSGRVEVPVGR-----DPNNRLRMIAVAGLVNSVKARHAASRYKVIEILAGGSCALV 339
Query: 300 ELSPLTGRKHQLRVHCAEVLGTPIVGDYKYG 330
E TGR HQ+R H A+ LG P++GD YG
Sbjct: 340 EWKLETGRTHQIRAH-AKYLGVPLLGDEVYG 369
>Medtr1g095580.1 | pseudouridine synthase Rlu family protein | HC |
chr1:43010317-43004442 | 20130731
Length = 370
Score = 60.1 bits (144), Expect = 4e-09, Method: Compositional matrix adjust.
Identities = 59/205 (28%), Positives = 95/205 (46%), Gaps = 18/205 (8%)
Query: 140 VIYKDPAILVLNKPPGMPV-QGGIDIKRSL------DVVAASCLKYEYSEPPRLVHRLDR 192
V+Y+D I+ LNKP G+ V GG+ +R++ + S ++ P VHRL R
Sbjct: 97 VLYEDDDIIALNKPSGLQVLPGGLFQQRTVLTQLQWETNNKSTIEAHQKPHPVPVHRLGR 156
Query: 193 DCSGILVMGRTQTSATVLHSIFREKTSSASDDLGTENRV--LQRKYRALVIGCPRRPRGL 250
SGIL+ +T+ + L S F + TS T + + + YRALV G +
Sbjct: 157 GTSGILLCAKTKLARARLASYFADGTSRIEGKGHTNQELGKIAKIYRALVSGIINNDQVT 216
Query: 251 VTASLGKVVVDN-GKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGYTWLELSPLTGRKH 309
+ +G V K + LS H + R + +S T +++ +GR H
Sbjct: 217 INQPIGMVKYPGVAKGLYVASQSGKPALSVVHVLE--RNVKENS---TLVQVKIQSGRPH 271
Query: 310 QLRVHCAEVLGTPIVGD--YKYGWQ 332
Q+R+H + +G P++GD Y G Q
Sbjct: 272 QIRIHLS-FIGHPLLGDPLYAVGGQ 295
>Medtr1g095580.2 | pseudouridine synthase Rlu family protein | HC |
chr1:43010317-43004472 | 20130731
Length = 340
Score = 60.1 bits (144), Expect = 4e-09, Method: Compositional matrix adjust.
Identities = 57/200 (28%), Positives = 93/200 (46%), Gaps = 16/200 (8%)
Query: 140 VIYKDPAILVLNKPPGMPV-QGGIDIKRSL------DVVAASCLKYEYSEPPRLVHRLDR 192
V+Y+D I+ LNKP G+ V GG+ +R++ + S ++ P VHRL R
Sbjct: 97 VLYEDDDIIALNKPSGLQVLPGGLFQQRTVLTQLQWETNNKSTIEAHQKPHPVPVHRLGR 156
Query: 193 DCSGILVMGRTQTSATVLHSIFREKTSSASDDLGTENRV--LQRKYRALVIGCPRRPRGL 250
SGIL+ +T+ + L S F + TS T + + + YRALV G +
Sbjct: 157 GTSGILLCAKTKLARARLASYFADGTSRIEGKGHTNQELGKIAKIYRALVSGIINNDQVT 216
Query: 251 VTASLGKVVVDN-GKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGYTWLELSPLTGRKH 309
+ +G V K + LS H + R + +S T +++ +GR H
Sbjct: 217 INQPIGMVKYPGVAKGLYVASQSGKPALSVVHVLE--RNVKENS---TLVQVKIQSGRPH 271
Query: 310 QLRVHCAEVLGTPIVGDYKY 329
Q+R+H + +G P++GD Y
Sbjct: 272 QIRIHLS-FIGHPLLGDPLY 290
>Medtr1g095580.3 | pseudouridine synthase Rlu family protein | HC |
chr1:43010317-43005492 | 20130731
Length = 312
Score = 59.7 bits (143), Expect = 5e-09, Method: Compositional matrix adjust.
Identities = 60/206 (29%), Positives = 98/206 (47%), Gaps = 20/206 (9%)
Query: 140 VIYKDPAILVLNKPPGMPV-QGGIDIKRSL------DVVAASCLKYEYSEPPRLVHRLDR 192
V+Y+D I+ LNKP G+ V GG+ +R++ + S ++ P VHRL R
Sbjct: 97 VLYEDDDIIALNKPSGLQVLPGGLFQQRTVLTQLQWETNNKSTIEAHQKPHPVPVHRLGR 156
Query: 193 DCSGILVMGRTQTSATVLHSIFREKTSSASDDLGTENRV--LQRKYRALVIGCPRRPRGL 250
SGIL+ +T+ + L S F + TS T + + + YRALV G +
Sbjct: 157 GTSGILLCAKTKLARARLASYFADGTSRIEGKGHTNQELGKIAKIYRALVSGIINNDQVT 216
Query: 251 VTASLGKVVVDNGKSDRITIVDNSS--LLSSQHAITEYRVIGSSSHGYTWLELSPLTGRK 308
+ +G V G + + + S LS H + R + +S T +++ +GR
Sbjct: 217 INQPIGMVKYP-GVAKGLYVASQSGKPALSVVHVLE--RNVKENS---TLVQVKIQSGRP 270
Query: 309 HQLRVHCAEVLGTPIVGD--YKYGWQ 332
HQ+R+H + +G P++GD Y G Q
Sbjct: 271 HQIRIHLS-FIGHPLLGDPLYAVGGQ 295
>Medtr3g098940.2 | pseudouridine synthase Rlu family protein | HC |
chr3:45332375-45325490 | 20130731
Length = 352
Score = 55.8 bits (133), Expect = 8e-08, Method: Compositional matrix adjust.
Identities = 52/191 (27%), Positives = 87/191 (45%), Gaps = 24/191 (12%)
Query: 140 VIYKDPAILVLNKPPGMPVQGGIDIKRSLDVVAASCLKYEYSEPPRL-VHRLDRDCSGIL 198
+++K+P +L + KP +PV +++ V L+ E+ P VHRLDR SG+L
Sbjct: 119 ILHKEPDVLTVCKPASVPVHPCGQYRKNTVV---GILQAEHGLAPLFPVHRLDRLVSGLL 175
Query: 199 VMGRTQTSATVLHSIFREKTSSASDDLGTENRVLQRKYRALVIGCPRRPRGLVTASLGKV 258
++ R T A FR++ + +++++Y A V+G +V A++
Sbjct: 176 IIARNATKA----DSFRQEIEAG---------LVKKQYIAKVVGEFPEDEIIVDANIDY- 221
Query: 259 VVDNGKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGYTWLELSPLTGRKHQLRVHCAEV 318
N + R T S + R+ + +H E P+TGR HQ+RVH +
Sbjct: 222 ---NAREGRSTAEVRDSTKGKVASTKFTRISSNGTHSIVLCE--PVTGRTHQIRVHL-QS 275
Query: 319 LGTPIVGDYKY 329
G PI D Y
Sbjct: 276 SGHPIANDMLY 286
>Medtr3g098940.1 | pseudouridine synthase Rlu family protein | HC |
chr3:45332375-45324673 | 20130731
Length = 380
Score = 55.5 bits (132), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 51/191 (26%), Positives = 86/191 (45%), Gaps = 24/191 (12%)
Query: 140 VIYKDPAILVLNKPPGMPVQGGIDIKRSLDVVAASCLKYEYSEPPRL-VHRLDRDCSGIL 198
+++K+P +L + KP +PV +++ L+ E+ P VHRLDR SG+L
Sbjct: 119 ILHKEPDVLTVCKPASVPVHPCGQYRKN---TVVGILQAEHGLAPLFPVHRLDRLVSGLL 175
Query: 199 VMGRTQTSATVLHSIFREKTSSASDDLGTENRVLQRKYRALVIGCPRRPRGLVTASLGKV 258
++ R T A FR++ + +++++Y A V+G +V A++
Sbjct: 176 IIARNATKA----DSFRQEIEAG---------LVKKQYIAKVVGEFPEDEIIVDANIDY- 221
Query: 259 VVDNGKSDRITIVDNSSLLSSQHAITEYRVIGSSSHGYTWLELSPLTGRKHQLRVHCAEV 318
N + R T S + R+ + +H E P+TGR HQ+RVH +
Sbjct: 222 ---NAREGRSTAEVRDSTKGKVASTKFTRISSNGTHSIVLCE--PVTGRTHQIRVH-LQS 275
Query: 319 LGTPIVGDYKY 329
G PI D Y
Sbjct: 276 SGHPIANDMLY 286