Miyakogusa Predicted Gene

Lj0g3v0314249.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0314249.1 Non Characterized Hit- tr|D7SP48|D7SP48_VITVI
Putative uncharacterized protein OS=Vitis vinifera
GN=,71.77,0,seg,NULL; HATPase_c_3,NULL; ATPase domain of HSP90
chaperone/DNA topoisomerase II/histidine kinase,A,CUFF.21216.1
         (344 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g076590.1 | histidine kinase-, DNA gyrase B | HC | chr8:32...   348   5e-96
Medtr3g077540.2 | histidine kinase-, DNA gyrase B | HC | chr3:34...   323   1e-88
Medtr3g077540.1 | histidine kinase-, DNA gyrase B | HC | chr3:34...   323   1e-88
Medtr8g096410.4 | histidine kinase-, DNA gyrase B, putative | HC...   303   1e-82
Medtr8g096410.1 | histidine kinase-, DNA gyrase B, putative | HC...   303   1e-82
Medtr8g096410.2 | histidine kinase-, DNA gyrase B, putative | HC...   303   1e-82
Medtr8g096410.3 | histidine kinase-, DNA gyrase B, putative | HC...   303   1e-82
Medtr4g105530.1 | histidine kinase-, DNA gyrase B | HC | chr4:43...   293   2e-79
Medtr8g076580.1 | histidine kinase-, DNA gyrase B | HC | chr8:32...   182   3e-46
Medtr5g024810.1 | CW-type zinc finger protein | HC | chr5:100112...    91   2e-18

>Medtr8g076590.1 | histidine kinase-, DNA gyrase B | HC |
           chr8:32453780-32471662 | 20130731
          Length = 512

 Score =  348 bits (893), Expect = 5e-96,   Method: Compositional matrix adjust.
 Identities = 164/189 (86%), Positives = 174/189 (92%)

Query: 155 DPNSIRKCMSLGYSSKKSKTTIGQYGNGFKTSTMRLGADAIVFSRAVNSSRATQSVGLLS 214
           DPNS+RKCMSLGYSSKKSKTTIGQYGNGFKTSTMRLGADAIVFSR+  S RATQSVGLLS
Sbjct: 40  DPNSLRKCMSLGYSSKKSKTTIGQYGNGFKTSTMRLGADAIVFSRSTRSGRATQSVGLLS 99

Query: 215 YTFLRTTGQDDVIVPMIDFDISTHWAEPIIYSSQDDWSDNLKTILDWSPFSSKEELMLQF 274
           YTFLR TGQDDVI+PMIDFDIS HW EPI+Y + DDWS NLKTILDWSPF+SKEELMLQF
Sbjct: 100 YTFLRRTGQDDVIIPMIDFDISGHWVEPIVYGTLDDWSANLKTILDWSPFTSKEELMLQF 159

Query: 275 DDIGSHGTKILIYNLWLNDEGIYELSFDDDAEDIMLRDETNHGDEKKLSKKTVQLQSHIS 334
           DDIGSHGTKILIYNLWLNDEGIYELSFDDDAEDIMLRDE  +G+ KKL K+TVQLQSHI 
Sbjct: 160 DDIGSHGTKILIYNLWLNDEGIYELSFDDDAEDIMLRDEAKNGNGKKLDKRTVQLQSHIF 219

Query: 335 YRIRHSLRV 343
           YRIR+SLR 
Sbjct: 220 YRIRYSLRA 228


>Medtr3g077540.2 | histidine kinase-, DNA gyrase B | HC |
           chr3:34846655-34833326 | 20130731
          Length = 643

 Score =  323 bits (828), Expect = 1e-88,   Method: Compositional matrix adjust.
 Identities = 163/289 (56%), Positives = 209/289 (72%), Gaps = 12/289 (4%)

Query: 59  SAPVYSRSFWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVDN 118
           +AP+ SR FWKAG Y  G + +     G   +  VHP FLHSNATSHKWAFGAIAEL+DN
Sbjct: 92  AAPL-SRQFWKAGSYDEGHASQIGVKDGK-NYLHVHPMFLHSNATSHKWAFGAIAELLDN 149

Query: 119 AVDEIQNGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSKKSKTTIGQ 178
           AVDEIQNGATFV VDK  N +D SP L  QDDGGGMDP ++R+CMS G+S K SK +IGQ
Sbjct: 150 AVDEIQNGATFVSVDKTSNPRDGSPALLIQDDGGGMDPEAMRRCMSFGFSDKNSKLSIGQ 209

Query: 179 YGNGFKTSTMRLGADAIVFSRAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMIDFDISTH 238
           YGNGFKTS+MRLGADAIVFSR +N+   TQS+GLLSYTFL  T  D ++VPM++++ +T 
Sbjct: 210 YGNGFKTSSMRLGADAIVFSRHLNNGILTQSIGLLSYTFLTQTQLDRIVVPMVNYEFNTS 269

Query: 239 WAEPIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDIGSHGTKILIYNLWLNDEGIYE 298
                + + ++ +  NL  +L WSP+SS+ +L+ QFD++GSHGTK+++YNLWLNDEGI E
Sbjct: 270 TGSLDMLNGKEHFKANLSLLLRWSPYSSEADLLKQFDNMGSHGTKVIVYNLWLNDEGITE 329

Query: 299 LSFDDDAEDIMLRDETNHGDEKKLSK----KTVQLQSHISYRIRHSLRV 343
           L+FD D +DI +       D KK+      K +Q + HI+ R R+SLRV
Sbjct: 330 LNFDTDPKDIRIA-----WDIKKIGTKPAWKRIQ-EEHIANRFRYSLRV 372


>Medtr3g077540.1 | histidine kinase-, DNA gyrase B | HC |
           chr3:34846699-34833381 | 20130731
          Length = 643

 Score =  323 bits (828), Expect = 1e-88,   Method: Compositional matrix adjust.
 Identities = 163/289 (56%), Positives = 209/289 (72%), Gaps = 12/289 (4%)

Query: 59  SAPVYSRSFWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVDN 118
           +AP+ SR FWKAG Y  G + +     G   +  VHP FLHSNATSHKWAFGAIAEL+DN
Sbjct: 92  AAPL-SRQFWKAGSYDEGHASQIGVKDGK-NYLHVHPMFLHSNATSHKWAFGAIAELLDN 149

Query: 119 AVDEIQNGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSKKSKTTIGQ 178
           AVDEIQNGATFV VDK  N +D SP L  QDDGGGMDP ++R+CMS G+S K SK +IGQ
Sbjct: 150 AVDEIQNGATFVSVDKTSNPRDGSPALLIQDDGGGMDPEAMRRCMSFGFSDKNSKLSIGQ 209

Query: 179 YGNGFKTSTMRLGADAIVFSRAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMIDFDISTH 238
           YGNGFKTS+MRLGADAIVFSR +N+   TQS+GLLSYTFL  T  D ++VPM++++ +T 
Sbjct: 210 YGNGFKTSSMRLGADAIVFSRHLNNGILTQSIGLLSYTFLTQTQLDRIVVPMVNYEFNTS 269

Query: 239 WAEPIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDIGSHGTKILIYNLWLNDEGIYE 298
                + + ++ +  NL  +L WSP+SS+ +L+ QFD++GSHGTK+++YNLWLNDEGI E
Sbjct: 270 TGSLDMLNGKEHFKANLSLLLRWSPYSSEADLLKQFDNMGSHGTKVIVYNLWLNDEGITE 329

Query: 299 LSFDDDAEDIMLRDETNHGDEKKLSK----KTVQLQSHISYRIRHSLRV 343
           L+FD D +DI +       D KK+      K +Q + HI+ R R+SLRV
Sbjct: 330 LNFDTDPKDIRIA-----WDIKKIGTKPAWKRIQ-EEHIANRFRYSLRV 372


>Medtr8g096410.4 | histidine kinase-, DNA gyrase B, putative | HC |
           chr8:40447933-40438223 | 20130731
          Length = 798

 Score =  303 bits (777), Expect = 1e-82,   Method: Compositional matrix adjust.
 Identities = 149/285 (52%), Positives = 204/285 (71%), Gaps = 13/285 (4%)

Query: 65  RSFWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVDNAVDEIQ 124
           + FWKAGDY   P+    S    ++H RVHPKFLHSNATSHKWA GA AEL+DNA+DE+ 
Sbjct: 124 KQFWKAGDYGGSPARAFESSTVGMDHVRVHPKFLHSNATSHKWALGAFAELLDNALDEVC 183

Query: 125 NGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSK-KSKTTIGQYGNGF 183
           NGAT+V VD + + KD S +L  +D+GGGMDP+ IR+CMSLGYS K K   TIGQYGNGF
Sbjct: 184 NGATYVNVDMLASKKDGSRMLLVEDNGGGMDPDKIRQCMSLGYSEKSKMANTIGQYGNGF 243

Query: 184 KTSTMRLGADAIVFS--RAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMIDFDISTHWAE 241
           KTSTMRLGAD IVFS  +  +  R T+S+GLLSYTFLR TG++D++VPM+D++      +
Sbjct: 244 KTSTMRLGADVIVFSCCQGKDGKRPTRSIGLLSYTFLRNTGKEDIVVPMLDYEKDGQGWK 303

Query: 242 PIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDIGSHGTKILIYNLWLNDEGIYELSF 301
            ++ +S DDW++N++T++ WSPFS + +L+ QF+ + + GT+++IYNLW +D+G  EL+F
Sbjct: 304 KMLRTSLDDWNNNVETVVQWSPFSDEADLLCQFNLLKNQGTRVIIYNLWEDDQGQLELNF 363

Query: 302 DDDAEDIML----RDETNHGDEKKLSKKTVQLQSHISYRIRHSLR 342
           D+D  DI +    RDE N     K+++        ++Y  RHSLR
Sbjct: 364 DEDPHDIQIQGVNRDEKN----IKMAQDYPNSTHFLTY--RHSLR 402


>Medtr8g096410.1 | histidine kinase-, DNA gyrase B, putative | HC |
           chr8:40447933-40438223 | 20130731
          Length = 804

 Score =  303 bits (777), Expect = 1e-82,   Method: Compositional matrix adjust.
 Identities = 149/285 (52%), Positives = 204/285 (71%), Gaps = 13/285 (4%)

Query: 65  RSFWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVDNAVDEIQ 124
           + FWKAGDY   P+    S    ++H RVHPKFLHSNATSHKWA GA AEL+DNA+DE+ 
Sbjct: 124 KQFWKAGDYGGSPARAFESSTVGMDHVRVHPKFLHSNATSHKWALGAFAELLDNALDEVC 183

Query: 125 NGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSK-KSKTTIGQYGNGF 183
           NGAT+V VD + + KD S +L  +D+GGGMDP+ IR+CMSLGYS K K   TIGQYGNGF
Sbjct: 184 NGATYVNVDMLASKKDGSRMLLVEDNGGGMDPDKIRQCMSLGYSEKSKMANTIGQYGNGF 243

Query: 184 KTSTMRLGADAIVFS--RAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMIDFDISTHWAE 241
           KTSTMRLGAD IVFS  +  +  R T+S+GLLSYTFLR TG++D++VPM+D++      +
Sbjct: 244 KTSTMRLGADVIVFSCCQGKDGKRPTRSIGLLSYTFLRNTGKEDIVVPMLDYEKDGQGWK 303

Query: 242 PIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDIGSHGTKILIYNLWLNDEGIYELSF 301
            ++ +S DDW++N++T++ WSPFS + +L+ QF+ + + GT+++IYNLW +D+G  EL+F
Sbjct: 304 KMLRTSLDDWNNNVETVVQWSPFSDEADLLCQFNLLKNQGTRVIIYNLWEDDQGQLELNF 363

Query: 302 DDDAEDIML----RDETNHGDEKKLSKKTVQLQSHISYRIRHSLR 342
           D+D  DI +    RDE N     K+++        ++Y  RHSLR
Sbjct: 364 DEDPHDIQIQGVNRDEKN----IKMAQDYPNSTHFLTY--RHSLR 402


>Medtr8g096410.2 | histidine kinase-, DNA gyrase B, putative | HC |
           chr8:40447933-40438223 | 20130731
          Length = 802

 Score =  303 bits (777), Expect = 1e-82,   Method: Compositional matrix adjust.
 Identities = 149/285 (52%), Positives = 204/285 (71%), Gaps = 13/285 (4%)

Query: 65  RSFWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVDNAVDEIQ 124
           + FWKAGDY   P+    S    ++H RVHPKFLHSNATSHKWA GA AEL+DNA+DE+ 
Sbjct: 124 KQFWKAGDYGGSPARAFESSTVGMDHVRVHPKFLHSNATSHKWALGAFAELLDNALDEVC 183

Query: 125 NGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSK-KSKTTIGQYGNGF 183
           NGAT+V VD + + KD S +L  +D+GGGMDP+ IR+CMSLGYS K K   TIGQYGNGF
Sbjct: 184 NGATYVNVDMLASKKDGSRMLLVEDNGGGMDPDKIRQCMSLGYSEKSKMANTIGQYGNGF 243

Query: 184 KTSTMRLGADAIVFS--RAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMIDFDISTHWAE 241
           KTSTMRLGAD IVFS  +  +  R T+S+GLLSYTFLR TG++D++VPM+D++      +
Sbjct: 244 KTSTMRLGADVIVFSCCQGKDGKRPTRSIGLLSYTFLRNTGKEDIVVPMLDYEKDGQGWK 303

Query: 242 PIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDIGSHGTKILIYNLWLNDEGIYELSF 301
            ++ +S DDW++N++T++ WSPFS + +L+ QF+ + + GT+++IYNLW +D+G  EL+F
Sbjct: 304 KMLRTSLDDWNNNVETVVQWSPFSDEADLLCQFNLLKNQGTRVIIYNLWEDDQGQLELNF 363

Query: 302 DDDAEDIML----RDETNHGDEKKLSKKTVQLQSHISYRIRHSLR 342
           D+D  DI +    RDE N     K+++        ++Y  RHSLR
Sbjct: 364 DEDPHDIQIQGVNRDEKN----IKMAQDYPNSTHFLTY--RHSLR 402


>Medtr8g096410.3 | histidine kinase-, DNA gyrase B, putative | HC |
           chr8:40447933-40438223 | 20130731
          Length = 827

 Score =  303 bits (777), Expect = 1e-82,   Method: Compositional matrix adjust.
 Identities = 149/285 (52%), Positives = 204/285 (71%), Gaps = 13/285 (4%)

Query: 65  RSFWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVDNAVDEIQ 124
           + FWKAGDY   P+    S    ++H RVHPKFLHSNATSHKWA GA AEL+DNA+DE+ 
Sbjct: 124 KQFWKAGDYGGSPARAFESSTVGMDHVRVHPKFLHSNATSHKWALGAFAELLDNALDEVC 183

Query: 125 NGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSK-KSKTTIGQYGNGF 183
           NGAT+V VD + + KD S +L  +D+GGGMDP+ IR+CMSLGYS K K   TIGQYGNGF
Sbjct: 184 NGATYVNVDMLASKKDGSRMLLVEDNGGGMDPDKIRQCMSLGYSEKSKMANTIGQYGNGF 243

Query: 184 KTSTMRLGADAIVFS--RAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMIDFDISTHWAE 241
           KTSTMRLGAD IVFS  +  +  R T+S+GLLSYTFLR TG++D++VPM+D++      +
Sbjct: 244 KTSTMRLGADVIVFSCCQGKDGKRPTRSIGLLSYTFLRNTGKEDIVVPMLDYEKDGQGWK 303

Query: 242 PIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDIGSHGTKILIYNLWLNDEGIYELSF 301
            ++ +S DDW++N++T++ WSPFS + +L+ QF+ + + GT+++IYNLW +D+G  EL+F
Sbjct: 304 KMLRTSLDDWNNNVETVVQWSPFSDEADLLCQFNLLKNQGTRVIIYNLWEDDQGQLELNF 363

Query: 302 DDDAEDIML----RDETNHGDEKKLSKKTVQLQSHISYRIRHSLR 342
           D+D  DI +    RDE N     K+++        ++Y  RHSLR
Sbjct: 364 DEDPHDIQIQGVNRDEKN----IKMAQDYPNSTHFLTY--RHSLR 402


>Medtr4g105530.1 | histidine kinase-, DNA gyrase B | HC |
           chr4:43806717-43795114 | 20130731
          Length = 654

 Score =  293 bits (750), Expect = 2e-79,   Method: Compositional matrix adjust.
 Identities = 158/298 (53%), Positives = 199/298 (66%), Gaps = 20/298 (6%)

Query: 54  TPASNSAPVYSRSFWKAGDY--VVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGA 111
           +P   SA    R FWKAG+Y   +G  V     + YL    VHP FLHSNATSHKW FGA
Sbjct: 80  SPQPISAAPLCRKFWKAGNYDNRLGSKVSSQCAKNYLH---VHPLFLHSNATSHKWVFGA 136

Query: 112 IAELVDNAVDEIQNGATFVKVDKIDNMKDNSPVLCFQDDGGGMDPNSIRKCMSLGYSSKK 171
           IAEL+DNAVDEI NGATFVKVDKI N KD SP L  QDDG GM P ++++CMS G+S K 
Sbjct: 137 IAELLDNAVDEIHNGATFVKVDKILNPKDGSPALLIQDDGNGMGPEAMQRCMSFGFSDKI 196

Query: 172 SKTTIGQYGNGFKTSTMRLGADAIVFSRAVNSSRATQSVGLLSYTFLRTTGQDDVIVPMI 231
           S+  IGQYGNGFKT +MRLGADAIVFS  VN    T+S+GLLSYTFL     D ++VPM+
Sbjct: 197 SEFAIGQYGNGFKTGSMRLGADAIVFSCHVNDMVCTKSIGLLSYTFLMRAQLDRIVVPMV 256

Query: 232 DFDI--STHWAEPIIYSSQDDWSDNLKTILDWSPFSSKEELMLQFDDI-GSHGTKILIYN 288
           ++    ST + E +  + Q+ +  N+  +LDWSPF S+ E++ QFDDI   HGTKI+I+N
Sbjct: 257 NYKCNPSTGYLEKL--NHQEHFKSNMSLLLDWSPFLSEAEIVKQFDDIENHHGTKIIIFN 314

Query: 289 LWLNDEGIYELSFDDDAEDIMLRDETNHGDEKKL----SKKTVQLQSHISYRIRHSLR 342
           LW ND+   EL FD D +DI++      GD KK+    ++K V  Q  ++ R+ HSLR
Sbjct: 315 LWFNDDENLELDFDTDPQDILIA-----GDVKKIDTVPARKRVNEQ-QLANRLHHSLR 366


>Medtr8g076580.1 | histidine kinase-, DNA gyrase B | HC |
           chr8:32449766-32453665 | 20130731
          Length = 138

 Score =  182 bits (463), Expect = 3e-46,   Method: Compositional matrix adjust.
 Identities = 94/150 (62%), Positives = 110/150 (73%), Gaps = 16/150 (10%)

Query: 1   MSLIPKTEKDDD-VPVVDLDSTENSXXXXXXXXXXXXXXXXXXXIQVEPPLPKNTPASNS 59
           MS++PKTEK+D  +PVVDLDS ++                      ++ P  +N P   S
Sbjct: 1   MSIVPKTEKNDTAIPVVDLDSDDDEGTSATMS-------------DIQLPFQQNPPELIS 47

Query: 60  APVYSRS--FWKAGDYVVGPSVKPASDQGYLEHARVHPKFLHSNATSHKWAFGAIAELVD 117
            P+ S+S  FWKAGDYV GPS KP+  QG+L+HARVHPKFLHSNATSHKWAFGAIAELVD
Sbjct: 48  PPLVSQSQSFWKAGDYVAGPSSKPSPSQGHLDHARVHPKFLHSNATSHKWAFGAIAELVD 107

Query: 118 NAVDEIQNGATFVKVDKIDNMKDNSPVLCF 147
           NAVDEIQNGATFVKVDK++NMKDNSPVLCF
Sbjct: 108 NAVDEIQNGATFVKVDKLENMKDNSPVLCF 137


>Medtr5g024810.1 | CW-type zinc finger protein | HC |
           chr5:10011299-9997842 | 20130731
          Length = 694

 Score = 90.5 bits (223), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 68/209 (32%), Positives = 105/209 (50%), Gaps = 21/209 (10%)

Query: 91  ARVHPKFLHSNATSHK-WAFGAIAELVDNAVD-EIQNGATFVKVDKIDNMKDNSPVLCFQ 148
            ++   +L +   +H  W FG IAELVDN+ D +      FV++ K+     + P+L   
Sbjct: 168 VKIDQSYLKTLGQAHSGWIFGGIAELVDNSSDAKATKMDIFVEMIKLKKSGKDVPMLSVI 227

Query: 149 DDGGGMDPNSIRKCMSLGY--SSKKSKTTIGQYGNGFKTSTMRLGADAIVFSRAVNSSRA 206
           DDG GM+   + K +SLG+  S    K  IG++G GFKT  MRLG D +V ++  NS   
Sbjct: 228 DDGQGMNHEEVIKMVSLGHKQSGYDDKDQIGRFGVGFKTGAMRLGRDVLVLTQTANS--- 284

Query: 207 TQSVGLLSYTFLRTTGQDDVIVPMIDFDISTHWAEPIIYSSQDDWSD-NLKTILDWSPFS 265
            +S+  LS +     G+D++ +P++ +       E    +  +  +  NLK I D SPF+
Sbjct: 285 -RSIAFLSQSL--NEGKDNIEIPIVSYCRQGQQMEVDPSAQSESLAKFNLKAIQDNSPFN 341

Query: 266 -----SKEELMLQFDDIGSHGTKILIYNL 289
                 K  L       G  GT+I I+NL
Sbjct: 342 KYLIGEKAALF-----CGGTGTQIYIWNL 365