Miyakogusa Predicted Gene
- Lj0g3v0269609.1
BLASTP 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Lj0g3v0269609.1 Non Characterized Hit- tr|I1JF46|I1JF46_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.51230
PE,78.07,0,alpha/beta-Hydrolases,NULL; FAMILY NOT NAMED,NULL;
Lipase_3,Lipase, class 3; no description,NULL,gene.g20921.t1.1
(228 letters)
Database: Medicago_aa4.0v1
62,319 sequences; 21,947,249 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Medtr4g077070.1 | phospholipase A1 | HC | chr4:29533232-29529469... 279 2e-75
Medtr7g096120.1 | phospholipase A1 | HC | chr7:38545562-38548632... 275 3e-74
Medtr7g096140.1 | phospholipase A1 | HC | chr7:38551976-38556530... 235 3e-62
Medtr1995s0010.1 | phospholipase A1-Igamma 2 | HC | scaffold1995... 184 5e-47
Medtr2g015690.1 | phospholipase A1 | HC | chr2:4677023-4674351 |... 150 8e-37
Medtr4g087810.1 | phospholipase A1 | HC | chr4:34549288-34547417... 149 1e-36
Medtr4g087830.1 | phospholipase A1 | HC | chr4:34558798-34555984... 144 6e-35
Medtr5g013230.1 | glycerolipase A1 | HC | chr5:4166141-4167996 |... 142 2e-34
Medtr4g080100.1 | phospholipase A1 | HC | chr4:31047715-31045592... 135 3e-32
Medtr7g081645.1 | glycerolipase A1 | HC | chr7:31303833-31305104... 125 3e-29
Medtr4g099510.1 | glycerolipase A1 | HC | chr4:41315525-41313834... 125 3e-29
Medtr7g006400.1 | phospholipase A1-Ialpha 2 | HC | chr7:682679-6... 124 6e-29
Medtr7g081620.1 | glycerolipase A1 | HC | chr7:31261779-31263131... 122 4e-28
Medtr2g020020.1 | alpha/beta-hydrolase superfamily protein | HC ... 50 1e-06
>Medtr4g077070.1 | phospholipase A1 | HC | chr4:29533232-29529469 |
20130731
Length = 534
Score = 279 bits (713), Expect = 2e-75, Method: Compositional matrix adjust.
Identities = 134/224 (59%), Positives = 166/224 (74%), Gaps = 8/224 (3%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRD---NSIVPVSVMSFSGPRVG 57
++ Y E++SIT+TGHSLG+ALA+LSAYDIAE G+N+ D + VP++V SF+GPRVG
Sbjct: 293 LQFYQGEKISITVTGHSLGAALAVLSAYDIAELGVNIIEDGDKTTNVPITVYSFAGPRVG 352
Query: 58 NARFKERLEMLGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAE---ALPWSYSHVGE 114
N FKER E LGVKVLR+ N+HD VP PG + NE K E + PWSY+HVG
Sbjct: 353 NLHFKERCEELGVKVLRIHNIHDKVPTVPGIITNEK--FQFQKYIEDTLSFPWSYAHVGT 410
Query: 115 ELVLDHKNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLK 174
E+ LDH+NSPFLK + D C HNLEVLLHL+DGYHGK ++F + T RD ALVNK+CD L+
Sbjct: 411 EIKLDHRNSPFLKENGDLGCTHNLEVLLHLMDGYHGKDKKFNMVTERDIALVNKSCDLLR 470
Query: 175 DHYLVPPHWRQDENKGMIRGNDGRWVQPERPKLDDHPEDMLHHL 218
+ VPPHWRQDENKGM++ DGRWV PERP L+ HP D+ HHL
Sbjct: 471 SEFGVPPHWRQDENKGMVQTADGRWVVPERPLLEAHPPDIAHHL 514
>Medtr7g096120.1 | phospholipase A1 | HC | chr7:38545562-38548632 |
20130731
Length = 503
Score = 275 bits (703), Expect = 3e-74, Method: Compositional matrix adjust.
Identities = 136/227 (59%), Positives = 172/227 (75%), Gaps = 4/227 (1%)
Query: 4 YHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARFKE 63
+ +EE+SIT+TGHSLGSA+A LSA+DIAETGLN++ + + VSV SFSGPRVGN +FK
Sbjct: 274 FPNEEVSITLTGHSLGSAMATLSAFDIAETGLNVRENGEKIHVSVFSFSGPRVGNVKFKG 333
Query: 64 RLEM-LGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEAL--PWSYSHVGEELVLDH 120
RLE LGVK+LRV N HD+VPK+PGFL NE P ++K AE + PW Y+HVG EL LDH
Sbjct: 334 RLEKHLGVKILRVHNKHDMVPKSPGFLINEKSPAWLLKFAEDIDIPWCYTHVGVELELDH 393
Query: 121 KNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLKDHYLVP 180
K SPFL P+ADA CAHNLE LH++DGYHG + + +T RD ALVNK+CD +KD + VP
Sbjct: 394 KISPFLNPNADAACAHNLEAHLHILDGYHGSNRGYEATTDRDIALVNKSCDFVKDEHYVP 453
Query: 181 PHWRQDENKGMIRGNDGRWVQPERPK-LDDHPEDMLHHLRQLGLTSS 226
P+WRQD N+ M++ DGRW+ RP+ +D H ED+ HL Q+GL SS
Sbjct: 454 PNWRQDLNRNMVKTEDGRWMLAHRPQVVDTHHEDLEPHLSQIGLISS 500
>Medtr7g096140.1 | phospholipase A1 | HC | chr7:38551976-38556530 |
20130731
Length = 461
Score = 235 bits (600), Expect = 3e-62, Method: Compositional matrix adjust.
Identities = 116/185 (62%), Positives = 143/185 (77%), Gaps = 3/185 (1%)
Query: 4 YHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARFKE 63
+ +EE+SIT+TGHSLGSA+A LSA+DIAET LN++ + + VSV SFSGPRVGN +FK
Sbjct: 274 FPNEEVSITLTGHSLGSAMATLSAFDIAETRLNVRENGEKIHVSVFSFSGPRVGNVKFKG 333
Query: 64 RLEM-LGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEAL--PWSYSHVGEELVLDH 120
RLE LGVK+LRV N HD+VPK+PGFL NE P ++K AE + PW Y+HVG EL LDH
Sbjct: 334 RLEKHLGVKILRVHNKHDMVPKSPGFLINEKSPAWLLKFAEDIDIPWCYTHVGVELELDH 393
Query: 121 KNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLKDHYLVP 180
K SPFL P+ADA CAHNLE LH++DGYHG + + +T RD ALVNKACD +KD + VP
Sbjct: 394 KISPFLNPNADAACAHNLEAHLHILDGYHGSNRGYEATTNRDIALVNKACDFVKDEHSVP 453
Query: 181 PHWRQ 185
P+WRQ
Sbjct: 454 PNWRQ 458
>Medtr1995s0010.1 | phospholipase A1-Igamma 2 | HC |
scaffold1995:495-1167 | 20130731
Length = 223
Score = 184 bits (468), Expect = 5e-47, Method: Compositional matrix adjust.
Identities = 93/146 (63%), Positives = 114/146 (78%), Gaps = 3/146 (2%)
Query: 4 YHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARFKE 63
+ +EE+SIT+TGHSLGSA+A LSA+DIAET LN++ + + VSV SFSGPRVGN +FK
Sbjct: 78 FPNEEVSITLTGHSLGSAMATLSAFDIAETRLNVRENGEKIHVSVFSFSGPRVGNVKFKG 137
Query: 64 RLEM-LGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAE--ALPWSYSHVGEELVLDH 120
RLE LGVK+LRV N HD+VPK+PGFL NE P ++K AE +PW Y+HVG EL LDH
Sbjct: 138 RLEKHLGVKILRVHNKHDMVPKSPGFLINEKSPAWLLKFAEDIDIPWCYTHVGVELELDH 197
Query: 121 KNSPFLKPDADAVCAHNLEVLLHLID 146
K SPFL P+ADA CAHNLE LH++D
Sbjct: 198 KISPFLNPNADAACAHNLEAHLHILD 223
>Medtr2g015690.1 | phospholipase A1 | HC | chr2:4677023-4674351 |
20130731
Length = 414
Score = 150 bits (380), Expect = 8e-37, Method: Compositional matrix adjust.
Identities = 91/216 (42%), Positives = 118/216 (54%), Gaps = 28/216 (12%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSI--VPVSVMSFSGPRVGN 58
+E Y +EE+SITITGHSLG+A+A L+A DI G N D S+ PV+ + F+ PRVG+
Sbjct: 222 VEKYKNEEISITITGHSLGAAIATLNAVDIVTNGFNKPSDPSLKASPVTAIVFASPRVGD 281
Query: 59 ARFKERLEMLG-VKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEALPWSYSHVGEELV 117
F++ + LR+ N D+VP P F+ YS VGEEL
Sbjct: 282 TNFQKLFSSYKDLSTLRIRNELDIVPNYP-FI------------------GYSDVGEELK 322
Query: 118 LDHKNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLKDHY 177
+D + S +LK + + HNLE LH + G G + F L RD ALVNK D LKD Y
Sbjct: 323 IDTRKSMYLKSPGNILSWHNLEAYLHGVAGTQGSKRVFKLEVNRDIALVNKTLDGLKDEY 382
Query: 178 LVPPHWRQDENKGMIRGNDGRWVQPERPKLDDHPED 213
LVP WR ENKGM++ DG W KL DH +D
Sbjct: 383 LVPVSWRVVENKGMVQQLDGSW------KLIDHEDD 412
>Medtr4g087810.1 | phospholipase A1 | HC | chr4:34549288-34547417 |
20130731
Length = 408
Score = 149 bits (377), Expect = 1e-36, Method: Compositional matrix adjust.
Identities = 88/218 (40%), Positives = 121/218 (55%), Gaps = 30/218 (13%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDN--SIVPVSVMSFSGPRVGN 58
+E+Y +EE+SIT+TGHSLG ALA +S+ DI NM ++ PV+ +F PRVGN
Sbjct: 213 VELYKNEEISITVTGHSLGGALATISSVDIVANKFNMPKEQPQKACPVTTFAFGAPRVGN 272
Query: 59 ARFKERL-EMLGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEALPWSYSHVGEELV 117
+ F++ + + L V N +D+VPK+ F Y VGEEL
Sbjct: 273 SYFQKIFSDHKDLSALFVRNENDIVPKSLTFF-------------------YYKVGEELE 313
Query: 118 LDHKNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLKDHY 177
+D + S +LK V AHN+EV LH I G G F L RD AL+NK+ D LKD Y
Sbjct: 314 IDTEESKYLKS---GVSAHNMEVYLHGIAGTQGSKGGFNLEVNRDIALLNKSNDGLKDEY 370
Query: 178 LVPPHWRQDENKGMIRGNDGRWVQPERPKLDDHPEDML 215
+P +WR ENKGM++ +DG W +DDH +D+L
Sbjct: 371 HIPENWRVVENKGMVQQSDGTW-----KLMDDHNDDVL 403
>Medtr4g087830.1 | phospholipase A1 | HC | chr4:34558798-34555984 |
20130731
Length = 400
Score = 144 bits (363), Expect = 6e-35, Method: Compositional matrix adjust.
Identities = 85/218 (38%), Positives = 122/218 (55%), Gaps = 30/218 (13%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDN--SIVPVSVMSFSGPRVGN 58
+E+Y +EE+SIT+TGHSLG ALA +S+ DI N+ + PV++ +F PRVGN
Sbjct: 204 VELYKNEEISITVTGHSLGGALATISSMDIVANKFNIPKGQPQKTCPVTLFAFGSPRVGN 263
Query: 59 ARFKERL-EMLGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEALPWSYSHVGEELV 117
+ F++ + ++ L + N +D+VP + L +YS VGEEL
Sbjct: 264 SNFEKIFSDNNDLRALFIRNNNDIVPSS-------------------LRLAYSKVGEELE 304
Query: 118 LDHKNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLKDHY 177
+D + S +LK V HN+EV LH I G G F L RD AL+NK+ D LKD Y
Sbjct: 305 IDTEKSKYLKS---GVSEHNMEVYLHGIAGTQGSKGGFNLEVNRDIALLNKSNDGLKDEY 361
Query: 178 LVPPHWRQDENKGMIRGNDGRWVQPERPKLDDHPEDML 215
+P +WR ENKGM++ +DG W +DDH +D+L
Sbjct: 362 HIPENWRVVENKGMVQQSDGTW-----KLMDDHNDDVL 394
>Medtr5g013230.1 | glycerolipase A1 | HC | chr5:4166141-4167996 |
20130731
Length = 548
Score = 142 bits (359), Expect = 2e-34, Method: Compositional matrix adjust.
Identities = 77/174 (44%), Positives = 99/174 (56%), Gaps = 16/174 (9%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNAR 60
+E+Y EELSIT+TGHSLG+ LA+L A +I+ N + PV+V SF GPRVGN
Sbjct: 328 IELYKGEELSITVTGHSLGATLALLVAEEISTCAPN------VPPVAVFSFGGPRVGNRA 381
Query: 61 FKERLEMLGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMK----------IAEALPWSYS 110
F E LE VKVLR+VN D++ + PG +E + + E P YS
Sbjct: 382 FGEHLEKKNVKVLRIVNTQDVITRVPGIFLSEELEEKIKNSKVVSGVVDMLEENTPLGYS 441
Query: 111 HVGEELVLDHKNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPA 164
HVG EL ++ K SP+LKPDAD C H+LE LHL+DGY F + R A
Sbjct: 442 HVGTELRVNTKMSPYLKPDADIACCHDLEAYLHLVDGYMASNCPFRANAKRSLA 495
>Medtr4g080100.1 | phospholipase A1 | HC | chr4:31047715-31045592 |
20130731
Length = 442
Score = 135 bits (340), Expect = 3e-32, Method: Compositional matrix adjust.
Identities = 77/209 (36%), Positives = 112/209 (53%), Gaps = 26/209 (12%)
Query: 4 YHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARFKE 63
Y +E S+ + GHSLG++L+I+SA+D+ E G+ + +PV+ F P+VGN F +
Sbjct: 254 YKNENPSVVLVGHSLGASLSIVSAFDLVENGV------TDIPVTAFVFGSPQVGNKAFND 307
Query: 64 RLEML-GVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEALPWSYSHVGEELVLDHKN 122
R + +KVL V NV DL+P PG L Y + G ELV+D +
Sbjct: 308 RFKKFQNLKVLHVRNVIDLIPHYPGKLL-----------------GYEYTGTELVIDTRK 350
Query: 123 SPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPALVNKACDCLKDHYLVPPH 182
S LK + HNL+ +LH++ G++G F + R ALVNK+CD LK+ VP
Sbjct: 351 STSLKDSKNPSDWHNLQAMLHIVAGWNGSDGEFEVKVKRSLALVNKSCDYLKEECHVPAS 410
Query: 183 WRQDENKGMIRGNDGRWVQPERPKLDDHP 211
W NKGM+R D WV + P+ +D P
Sbjct: 411 WWVATNKGMVRREDEEWV--DAPEKEDLP 437
>Medtr7g081645.1 | glycerolipase A1 | HC | chr7:31303833-31305104 |
20130731
Length = 423
Score = 125 bits (315), Expect = 3e-29, Method: Compositional matrix adjust.
Identities = 71/160 (44%), Positives = 94/160 (58%), Gaps = 16/160 (10%)
Query: 2 EIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARF 61
+IY EELS+TITGHSLG+ALA L AYD+ K + V+V+SF GPRVGN F
Sbjct: 276 QIYRQEELSLTITGHSLGAALATLIAYDV-------KISVPKLMVTVISFGGPRVGNRNF 328
Query: 62 KERLEMLGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEALPWSYSHVGEELVLDHK 121
++ LE G KVLR+VN D++ K PGF+F + + PW Y+ VG+EL L +
Sbjct: 329 RQHLEKQGTKVLRIVNSDDVITKLPGFVFFDD--------VDKTPWMYAEVGKELRLCSR 380
Query: 122 NSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGR 161
+SP+L A C H L+ LHL+DG+ F S R
Sbjct: 381 DSPYLGSTNVATC-HELQTYLHLVDGFISSRCPFRASAKR 419
>Medtr4g099510.1 | glycerolipase A1 | HC | chr4:41315525-41313834 |
20130731
Length = 521
Score = 125 bits (315), Expect = 3e-29, Method: Compositional matrix adjust.
Identities = 77/173 (44%), Positives = 101/173 (58%), Gaps = 15/173 (8%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNAR 60
ME+Y E LSIT+TGHSLG+ALA+L A DI+ N + PV+V SF GP+VGN
Sbjct: 314 MEVYEGETLSITVTGHSLGAALALLVADDISTCNPN------VPPVAVFSFGGPKVGNKA 367
Query: 61 FKERLEMLGVKVLRVVNVHDLVPKTPGFLFNEH-----RPL----AVMKIAEALPWSYSH 111
+ ++ VKVLR+VN D++ + PG L +E R V + E P +Y+H
Sbjct: 368 YGNKITSQNVKVLRIVNSQDVITRVPGMLLSEEFEQKLRSFNFGGVVDMLDEKTPLAYTH 427
Query: 112 VGEELVLDHKNSPFLKPDADAVCAHNLEVLLHLIDGYHGKGQRFILSTGRDPA 164
VG EL +D K SPFLKPDAD C H+LE LHL+DG+ F + R A
Sbjct: 428 VGSELRVDTKMSPFLKPDADMACCHDLEAYLHLVDGFLASNCPFRANAKRSLA 480
>Medtr7g006400.1 | phospholipase A1-Ialpha 2 | HC |
chr7:682679-684360 | 20130731
Length = 506
Score = 124 bits (312), Expect = 6e-29, Method: Compositional matrix adjust.
Identities = 69/143 (48%), Positives = 93/143 (65%), Gaps = 10/143 (6%)
Query: 6 DEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARFKERL 65
++ +SI++ GHS+GSALAIL AYDI+E GLN K D + V+V SF GPRVGN FK+R
Sbjct: 296 EKNVSISLAGHSMGSALAILLAYDISELGLNKKNDKNDASVTVFSFGGPRVGNLEFKKRC 355
Query: 66 EMLGVKVLRVVNVHDLVPKTPGFLFNEHRPLAVMKIAEALPWS---YSHVGEELVLDHKN 122
E LGVKVLR+ NV+D + K PG +FNE+ + +M PWS Y+HVG EL+LD N
Sbjct: 356 EELGVKVLRISNVNDPITKLPGVVFNENFRV-LMGGRYEFPWSCSCYAHVGVELMLDFFN 414
Query: 123 SPFLKPDADAVCAHNLEVLLHLI 145
+ C H+L+ + L+
Sbjct: 415 M------QNPSCVHDLDTYIGLL 431
>Medtr7g081620.1 | glycerolipase A1 | HC | chr7:31261779-31263131 |
20130731
Length = 450
Score = 122 bits (305), Expect = 4e-28, Method: Compositional matrix adjust.
Identities = 72/167 (43%), Positives = 99/167 (59%), Gaps = 27/167 (16%)
Query: 1 MEIYHDEELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVP-VSVMSFSGPRVGNA 59
++ Y E LS TITGHSLG+ALAIL+A+DI +T + K P V+V+SF GPRVGN
Sbjct: 275 LKTYKGENLSFTITGHSLGAALAILTAHDI-KTYFDQK------PLVTVISFGGPRVGNK 327
Query: 60 RFKERLEMLGVKVLRVVNVHDLVPKTPGFLFNEH------------------RPLAVMKI 101
F+ +LE G+KVLR+VN D++ K PGF+ ++ P + K
Sbjct: 328 SFRLKLEKEGIKVLRIVNSDDVITKMPGFVLDDKVEESVYDMEGNNDDMAWFLPRWIQKR 387
Query: 102 AEALPWSYSHVGEELVLDHKNSPFLKPDADAVCAHNLEVLLHLIDGY 148
+ W YS VGEEL + +NSP+LK A C H+L+ LHL+DG+
Sbjct: 388 VKETQWVYSEVGEELRVCSRNSPYLKGVNIATC-HDLKTYLHLVDGF 433
>Medtr2g020020.1 | alpha/beta-hydrolase superfamily protein | HC |
chr2:6625967-6631759 | 20130731
Length = 358
Score = 50.4 bits (119), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 28/83 (33%), Positives = 41/83 (49%), Gaps = 7/83 (8%)
Query: 8 ELSITITGHSLGSALAILSAYDIAETGLNMKRDNSIVPVSVMSFSGPRVGNARFKERLEM 67
++ I GHS+G A+A D+ +N + N V VM+F PR+GN F
Sbjct: 166 DIPIIALGHSMGGAMAAFCGLDLT---VNKQEKN----VQVMTFGQPRIGNGVFVSLYSK 218
Query: 68 LGVKVLRVVNVHDLVPKTPGFLF 90
L +RV N HD+VP P + +
Sbjct: 219 LVPNTIRVTNDHDIVPHLPPYYY 241