Miyakogusa Predicted Gene

Lj0g3v0181439.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0181439.1 Non Characterized Hit- tr|I3S4V5|I3S4V5_LOTJA
Uncharacterized protein OS=Lotus japonicus PE=2
SV=1,99.23,0,SpoU_methylase,tRNA/rRNA methyltransferase, SpoU; no
description,NULL; SUBFAMILY NOT NAMED,NULL; RNA,CUFF.11526.1
         (259 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr3g027870.1 | tRNA/rRNA methyltransferase SpoU family protei...   400   e-112
Medtr3g027870.2 | tRNA/rRNA methyltransferase SpoU family protei...   298   3e-81

>Medtr3g027870.1 | tRNA/rRNA methyltransferase SpoU family protein |
           HC | chr3:8786548-8791874 | 20130731
          Length = 278

 Score =  400 bits (1028), Expect = e-112,   Method: Compositional matrix adjust.
 Identities = 206/261 (78%), Positives = 219/261 (83%), Gaps = 20/261 (7%)

Query: 14  PFRFRSKVSLFRPLFTSLHFKPIT---------HFSPRLCSFCSLNGSAE-----TSKEG 59
           P+ FRSK+SLFR     LHFKP +         H  P L S  + NGS       +SKEG
Sbjct: 23  PYHFRSKLSLFR----QLHFKPTSPFPSISSPPHLLP-LSSSLATNGSVAAENEISSKEG 77

Query: 60  SLPQGVGGAVKEASHSRILQVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGYKVDDTK 119
           SLPQGVG  V +ASHS+ILQVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGYKVDDTK
Sbjct: 78  SLPQGVGEGVNQASHSKILQVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGYKVDDTK 137

Query: 120 LKRAGLDYWPYVVVKIHNSWEDFRDYFRQQEGEKRLLAFTKRGTKIHSEFSYKKGDYLLF 179
           LKRAGLDYWPYVVVKIH+SWE FRDYF QQEGEKRLLAFTKRGTKIHS+FSY+KGDYLLF
Sbjct: 138 LKRAGLDYWPYVVVKIHDSWEGFRDYFLQQEGEKRLLAFTKRGTKIHSDFSYRKGDYLLF 197

Query: 180 GAETTGLPPEALLDCKTKPFGGGTLKIPMVETYVRCLNLSVSVGIALYEASRQLNYEQFQ 239
           GAETTGLPPEALLDCKT+PFGGGT+KIPMVETYVRCLNLSVSVGIALYEASRQLNYE  Q
Sbjct: 198 GAETTGLPPEALLDCKTEPFGGGTIKIPMVETYVRCLNLSVSVGIALYEASRQLNYESLQ 257

Query: 240 IPSETCIPT-EETLITEDILA 259
           IPSETCI T EE+ ITEDI  
Sbjct: 258 IPSETCIDTEEESFITEDIFG 278


>Medtr3g027870.2 | tRNA/rRNA methyltransferase SpoU family protein |
           HC | chr3:8786548-8791778 | 20130731
          Length = 238

 Score =  298 bits (763), Expect = 3e-81,   Method: Compositional matrix adjust.
 Identities = 169/261 (64%), Positives = 181/261 (69%), Gaps = 60/261 (22%)

Query: 14  PFRFRSKVSLFRPLFTSLHFKPIT---------HFSPRLCSFCSLNGSAE-----TSKEG 59
           P+ FRSK+SLFR     LHFKP +         H  P L S  + NGS       +SKEG
Sbjct: 23  PYHFRSKLSLFR----QLHFKPTSPFPSISSPPHLLP-LSSSLATNGSVAAENEISSKEG 77

Query: 60  SLPQGVGGAVKEASHSRILQVVLVSPQIPGNTGCIARTCAASAVGLHLVGPLGYKVDDTK 119
           SLPQGVG  V +ASHS+ILQVVLVSPQIPGNTGCIARTCAASAVGLHLVG          
Sbjct: 78  SLPQGVGEGVNQASHSKILQVVLVSPQIPGNTGCIARTCAASAVGLHLVG---------- 127

Query: 120 LKRAGLDYWPYVVVKIHNSWEDFRDYFRQQEGEKRLLAFTKRGTKIHSEFSYKKGDYLLF 179
                                         EGEKRLLAFTKRGTKIHS+FSY+KGDYLLF
Sbjct: 128 ------------------------------EGEKRLLAFTKRGTKIHSDFSYRKGDYLLF 157

Query: 180 GAETTGLPPEALLDCKTKPFGGGTLKIPMVETYVRCLNLSVSVGIALYEASRQLNYEQFQ 239
           GAETTGLPPEALLDCKT+PFGGGT+KIPMVETYVRCLNLSVSVGIALYEASRQLNYE  Q
Sbjct: 158 GAETTGLPPEALLDCKTEPFGGGTIKIPMVETYVRCLNLSVSVGIALYEASRQLNYESLQ 217

Query: 240 IPSETCIPT-EETLITEDILA 259
           IPSETCI T EE+ ITEDI  
Sbjct: 218 IPSETCIDTEEESFITEDIFG 238