Miyakogusa Predicted Gene

Lj0g3v0167619.1

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0167619.1 Non Characterized Hit- tr|F6HVA5|F6HVA5_VITVI
Putative uncharacterized protein OS=Vitis vinifera GN=,63.3,0,no
description,NULL; DNA-DIRECTED RNA POLYMERASE III SUBUNIT 2,NULL;
DNA-DIRECTED RNA POLYMERASE I S,gene.g12858.t1.1
         (287 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr8g023445.1 | DNA-directed RNA polymerase | LC | chr8:845866...   367   e-102
Medtr1g023500.1 | DNA-directed RNA polymerase subunit beta | HC ...   123   2e-28
Medtr8g023440.1 | RNA polymerase Rpb2 domain 3 protein | HC | ch...    59   6e-09

>Medtr8g023445.1 | DNA-directed RNA polymerase | LC |
            chr8:8458667-8488921 | 20130731
          Length = 2002

 Score =  367 bits (942), Expect = e-102,   Method: Compositional matrix adjust.
 Identities = 202/321 (62%), Positives = 212/321 (66%), Gaps = 90/321 (28%)

Query: 1    MERKGMTQVLQRLSFIGFLGQMTRVSPQFEKSRKVSGPRALQPSQ----VSCN-PVGEAC 55
            M + G+TQVLQRLSFI  LGQMTRV PQFEKSRKVSGPRALQPSQ      C+ P GE+C
Sbjct: 1297 MHKSGVTQVLQRLSFISALGQMTRVQPQFEKSRKVSGPRALQPSQWGMLCPCDTPEGESC 1356

Query: 56   GLVKNLALMTHVTTDEEEAPLISLISGAKSITNLTDLYNQLLGLRRSPEQILFLGVWRGG 115
            GLVKNLALMTHVTTDEEE PLISL                                    
Sbjct: 1357 GLVKNLALMTHVTTDEEEGPLISL------------------------------------ 1380

Query: 116  GCYYLGVEDMEYLSGEELHTPDSFLVIFNGLILGKHRRPRGFVTAMRKLRRAGKIGEFVS 175
             CY LGVE+ME+LSGEELHTP+SFLVIFNG+ILGKHRRP+ F TAMRKLRRA  IGEFVS
Sbjct: 1381 -CYLLGVENMEHLSGEELHTPNSFLVIFNGMILGKHRRPQRFATAMRKLRRACLIGEFVS 1439

Query: 176  VYVNEKQ-----------------------------------DGVRTFDDFLRDGLIEYL 200
            VYVNEKQ                                   DGV  F DFLR+GLIEYL
Sbjct: 1440 VYVNEKQCCVYLASDGGRVCRPLVIADNGISRVKAHHMKELKDGVCAFRDFLRNGLIEYL 1499

Query: 201  DVNEENNALIALYEGDATSETTHIEIEPFTILGVIAGLIPYPHHNQSPRNTYQVVSQSLP 260
            DVNEENNALIALYE DAT ETTHIEIEPFTILGVIAGLIPYPHHNQSPRNTYQ       
Sbjct: 1500 DVNEENNALIALYEKDATPETTHIEIEPFTILGVIAGLIPYPHHNQSPRNTYQ------- 1552

Query: 261  SLFLYKCAMGKQAMGNIAYNQ 281
                  CAMGKQAMGNIAYNQ
Sbjct: 1553 ------CAMGKQAMGNIAYNQ 1567


>Medtr1g023500.1 | DNA-directed RNA polymerase subunit beta | HC |
           chr1:7523400-7533496 | 20130731
          Length = 1195

 Score =  123 bits (309), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 99/328 (30%), Positives = 135/328 (41%), Gaps = 107/328 (32%)

Query: 3   RKGMTQVLQRLSFIGFLGQMTRVSPQFEKSRKVSGPRALQPSQ--VSC---NPVGEACGL 57
           R G++QVL RL++   L  + R++    +  K++ PR L  SQ  + C    P G+ACGL
Sbjct: 470 RAGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHNSQWGMMCPAETPEGQACGL 529

Query: 58  VKNLALMTHVTTDEEEAPLISLISGAKSITNLTDLYNQLLGLRRSPEQILFLGVWRGGGC 117
           VKNLALM ++T      P++                              FL  W     
Sbjct: 530 VKNLALMVYITVGSAAYPILE-----------------------------FLEEW----- 555

Query: 118 YYLGVEDMEYLSGEELHTPDSFLVIFNGLILGKHRRPRGFVTAMRKLRRAGKIGEFVSVY 177
              G E+ E +S   +  P +  +  NG  +G HR P   V  +RKLRR   +   V V 
Sbjct: 556 ---GTENFEEISPAVI--PQATKIFVNGCWMGIHRDPDMLVRTLRKLRRRVDVNTEVGVV 610

Query: 178 VNEKQDGVRTFDDFLR---------------------------------------DGLIE 198
            + +   +R + D+ R                                        G IE
Sbjct: 611 RDIRLKELRIYTDYGRCSRPLFIVDKQRLLIKKKDIHSLQQRESPEDGGWHDLVSKGFIE 670

Query: 199 YLDVNEENNALIALYEGD----------ATSET-THIEIEPFTILGVIAGLIPYPHHNQS 247
           Y+D  EE   +I++   D          A S+T TH EI P  ILGV A +IP+P HNQS
Sbjct: 671 YIDTEEEETTMISMTINDLVQARLNPEEAYSDTYTHCEIHPSLILGVCASIIPFPDHNQS 730

Query: 248 PRNTYQVVSQSLPSLFLYKCAMGKQAMG 275
           PRNTYQ              AMGKQAMG
Sbjct: 731 PRNTYQ-------------SAMGKQAMG 745


>Medtr8g023440.1 | RNA polymerase Rpb2 domain 3 protein | HC |
           chr8:8454247-8456089 | 20130731
          Length = 181

 Score = 58.9 bits (141), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 27/33 (81%), Positives = 29/33 (87%)

Query: 49  NPVGEACGLVKNLALMTHVTTDEEEAPLISLIS 81
            P GE CGLVKNLALMTHVT DEEEAPLISL++
Sbjct: 108 TPEGEYCGLVKNLALMTHVTADEEEAPLISLLA 140