Miyakogusa Predicted Gene

Lj0g3v0133059.1
Show Alignment:  

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0133059.1 Non Characterized Hit- tr|I1LV45|I1LV45_SOYBN
Uncharacterized protein OS=Glycine max GN=Gma.53853
PE,80.43,0,EF-hand,NULL; seg,NULL; EF_HAND_1,EF-Hand 1,
calcium-binding site; EF-hand, calcium binding motif,Ca,CUFF.8133.1
         (712 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr2g078350.1 | calcium-binding EF-hand protein | HC | chr2:32...   723   0.0  
Medtr2g078340.1 | calcium-binding EF-hand protein | HC | chr2:32...   605   e-173
Medtr2g078240.1 | calcium-binding EF-hand protein | HC | chr2:32...   212   9e-55
Medtr2g078250.1 | calcium ion-binding protein, putative | HC | c...    86   1e-16

>Medtr2g078350.1 | calcium-binding EF-hand protein | HC |
           chr2:32631917-32635869 | 20130731
          Length = 509

 Score =  723 bits (1867), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 356/497 (71%), Positives = 421/497 (84%), Gaps = 4/497 (0%)

Query: 137 LVVTGLSSDKESAQEYASTGVGLLAGSSILLLTVVWGTCVIIGKQKLKDDLNSVSSTSSI 196
           ++VTGLSSDKESAQEYASTGVGLLAGSSILLLTVVWGTCVI+GKQ L++D +S  +++S 
Sbjct: 1   MLVTGLSSDKESAQEYASTGVGLLAGSSILLLTVVWGTCVIVGKQSLRNDSHSSDTSNSS 60

Query: 197 -GRIKKTLTGYGITMDVETRKMAIIMVFSVIPLILMQIPKVFQLSSTPRDVTLMVSLIIS 255
            GRIK+ LTGYGITMDV+TRKMA IM+FSVIPL++MQIP +FQ S+T  +VTLM+SLII+
Sbjct: 61  TGRIKQALTGYGITMDVDTRKMARIMLFSVIPLLIMQIPTLFQFSTTLHNVTLMISLIIA 120

Query: 256 VAFLISYFIYQVFKPQIEKTRLEYIKHDDLILRIFQRVEKQTLQKILTEDGTPNITAISG 315
           V FL+SYFIYQ+FKPQIEKTRLEYIKHD+LILRIF+RVEKQTLQ ILTEDGTP++ AI+ 
Sbjct: 121 VTFLVSYFIYQIFKPQIEKTRLEYIKHDELILRIFERVEKQTLQTILTEDGTPDVDAINR 180

Query: 316 LYHEISQRGNKDLLTSEVKELLLGNNLTDTNIQDEKIADMLKVFDRNGDQIITKEEFVTG 375
           LYHE +Q G + L  S+V+ELL GNNLT T I+DE+I DMLKVFD++GDQIITKEEFV G
Sbjct: 181 LYHEFNQHGREHLSPSDVRELLFGNNLTSTEIRDEQITDMLKVFDKDGDQIITKEEFVNG 240

Query: 376 LTEYINQSKHALDQKYLPKESMNKMYQTFIKPWIEHVRKERELKGHLISGVLKHAQDDMV 435
           +TEY NQ+KHAL ++Y+PKE+M++MYQ+FIKPWIEH RKERELK HLISGVL HAQ D V
Sbjct: 241 VTEYFNQTKHALHKQYIPKENMSRMYQSFIKPWIEHTRKERELKEHLISGVLSHAQSDRV 300

Query: 436 GRLLTAEGTPDTTAIRRLFEQVDTNGDNHVSRSELDKIVKEIHFGKTVDVEEAAAKLLQD 495
           GRLL  +GTPD  AI+R FE++D+N D  VSRSEL+  VK+I FGKT   EEA  K LQ 
Sbjct: 301 GRLLKDDGTPDKDAIKRFFEEIDSNDDKSVSRSELENAVKKIKFGKT--AEEAVTKFLQV 358

Query: 496 LDLNRDDEISENEFVEGLSKWIXXXX-XXXXXXXXXXQGTHQTWEEVEKVMEENQQKGIS 554
           LD+N D+EISE EFV+G+++ I                 THQTWE+VEKVMEENQ KG++
Sbjct: 359 LDVNGDNEISETEFVDGITELINSYFGQDASSKSPSHHETHQTWEDVEKVMEENQTKGVT 418

Query: 555 AWLAAIGYVVLGITMLALLAEPLIASVQKFSEAAGLSSFFISFIIVPLATNFREATNAIK 614
           AWL AI YVVLGITML++LAEPLIASVQKFSEAAG+SSFFISFI+VPLATNFREAT+AIK
Sbjct: 419 AWLEAIAYVVLGITMLSILAEPLIASVQKFSEAAGISSFFISFILVPLATNFREATSAIK 478

Query: 615 EASHKKSGNTSHTMYEI 631
           EASHKKS NTSHTMYE+
Sbjct: 479 EASHKKSSNTSHTMYEV 495


>Medtr2g078340.1 | calcium-binding EF-hand protein | HC |
           chr2:32614673-32617960 | 20130731
          Length = 413

 Score =  605 bits (1561), Expect = e-173,   Method: Compositional matrix adjust.
 Identities = 291/376 (77%), Positives = 324/376 (86%), Gaps = 1/376 (0%)

Query: 266 QVFKPQIEKTRLEYIKHDDLILRIFQRVEKQTLQKILTEDGTPNITAISGLYHEISQRGN 325
           Q+FKPQIEKTRLEYIKHDDLILRIFQRVEKQTLQKILTEDGTPN+ AI+GLYHE SQ G 
Sbjct: 2   QLFKPQIEKTRLEYIKHDDLILRIFQRVEKQTLQKILTEDGTPNVAAINGLYHEFSQHG- 60

Query: 326 KDLLTSEVKELLLGNNLTDTNIQDEKIADMLKVFDRNGDQIITKEEFVTGLTEYINQSKH 385
           K L TSEVKELLLGNNL DTNI++++IADMLK FD+NGDQIITKEEFV+GLTEYINQ+KH
Sbjct: 61  KHLSTSEVKELLLGNNLADTNIREDQIADMLKAFDKNGDQIITKEEFVSGLTEYINQTKH 120

Query: 386 ALDQKYLPKESMNKMYQTFIKPWIEHVRKERELKGHLISGVLKHAQDDMVGRLLTAEGTP 445
           ALD+KYLPKESMNKMYQ+FIKPWIEHVRKERELKG LISGVL HAQ+DMVGR+L  +GT 
Sbjct: 121 ALDRKYLPKESMNKMYQSFIKPWIEHVRKERELKGQLISGVLSHAQNDMVGRMLHDDGTA 180

Query: 446 DTTAIRRLFEQVDTNGDNHVSRSELDKIVKEIHFGKTVDVEEAAAKLLQDLDLNRDDEIS 505
           D  AI+RLFEQVD +GDNHVSRSEL+K+V+E HFGK VD EEA +KLL DLD N+D+EIS
Sbjct: 181 DKDAIKRLFEQVDGDGDNHVSRSELEKVVRETHFGKAVDAEEAVSKLLHDLDANKDNEIS 240

Query: 506 ENEFVEGLSKWIXXXXXXXXXXXXXXQGTHQTWEEVEKVMEENQQKGISAWLAAIGYVVL 565
           ENEFV+G +KWI                THQTWE+VEKVMEENQ KG++AWL AI YV+L
Sbjct: 241 ENEFVDGFTKWINSNSSKPGSSKSSSHETHQTWEDVEKVMEENQTKGVTAWLEAIAYVML 300

Query: 566 GITMLALLAEPLIASVQKFSEAAGLSSFFISFIIVPLATNFREATNAIKEASHKKSGNTS 625
           GI ML+LLAEPLIASVQKFSEAAG+SSFFISFI+VPLATNFREAT+AIKEASHKKS NTS
Sbjct: 301 GIAMLSLLAEPLIASVQKFSEAAGISSFFISFILVPLATNFREATSAIKEASHKKSSNTS 360

Query: 626 HTMYEIYGAVFMNNIL 641
           HTMYE Y     N  L
Sbjct: 361 HTMYEEYSDGLHNKRL 376


>Medtr2g078240.1 | calcium-binding EF-hand protein | HC |
           chr2:32541026-32538200 | 20130731
          Length = 443

 Score =  212 bits (540), Expect = 9e-55,   Method: Compositional matrix adjust.
 Identities = 124/323 (38%), Positives = 180/323 (55%), Gaps = 18/323 (5%)

Query: 400 MYQTFIKPWIEHVRKERELKGHLISGVLKHAQDDMVGRLLTAEGTPDTTAIRRLFEQVDT 459
           +YQ F +PWI+  + E     H+I G+L+H +   +GRLL  +G PD   I++LF  +D 
Sbjct: 115 LYQIF-QPWIQRRKLEYIKHKHVIVGLLRHLKMRSLGRLLKDDGQPDKEVIKKLFTTIDE 173

Query: 460 NGDNHVSRSELDKIVKEIHFGKT-VDVEEAAAKLLQDLDLNRDDEISENEFVEGLSKWIX 518
           N D H++  E+  +V  I F +  +D ++A  K++ D D +R+  I E EFV G+ +W+ 
Sbjct: 174 NKDGHLTHGEMRALVVGIQFEEIDLDHDDAVTKIMADFDTSRNQLIDEGEFVNGVCRWLL 233

Query: 519 XXXXXXXXXXXXXQGT-------HQTWEEVEKVMEENQQKGISA-------WLA--AIGY 562
                          T       H   +    +++   Q    A       W++  A  +
Sbjct: 234 ATQRSRVASGDAGPHTVKFLSDFHTETKREHDLLDVGGQSDEGAEGVENARWISIKAGLF 293

Query: 563 VVLGITMLALLAEPLIASVQKFSEAAGLSSFFISFIIVPLATNFREATNAIKEASHKKSG 622
           ++LG  + A  A+PL+  V  FS+A  + +FFISFI +PLATN  EA +AI  AS  K  
Sbjct: 294 LLLGSLIAAAFADPLVDVVDNFSDATSIPAFFISFIFLPLATNSSEAVSAIIFASRDKRQ 353

Query: 623 NTSHTMYEIYGAVFMNNILGFVVISILIYVREITWEFSADVLVVAIVCAVMGLAASFFHT 682
             S T  EIYGAV MNN+L   V   L+YVR +TW+FS++VLV+ IVC VMG  ASF   
Sbjct: 354 TASLTFSEIYGAVTMNNVLCLSVFLALVYVRGLTWDFSSEVLVILIVCIVMGAFASFRTV 413

Query: 683 FPVWTSIPAYLMYLLSLLMVFVL 705
           FP+WTSI A L+Y  SL +V+VL
Sbjct: 414 FPLWTSITAILLYPFSLALVYVL 436



 Score =  125 bits (313), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 65/217 (29%), Positives = 130/217 (59%), Gaps = 11/217 (5%)

Query: 170 VVWG-----TCVIIGKQKLKDDLNSVSSTSSIGRIKKTLTGYGITMDVETRKMAIIMVFS 224
           +VW      +C   GK  ++D + ++ ST + G     LTG G++ D+ T   A IM+ S
Sbjct: 21  IVWAFRKYRSCSKSGKCDIEDSI-ALDSTDTRGF---HLTGSGVSTDIWTSYAARIMIIS 76

Query: 225 VIPLILMQIPKVFQLSSTPRDVTLMVSLIISVAFLISYFIYQVFKPQIEKTRLEYIKHDD 284
           VIP +++Q+P++   S++ R + +++  ++SV  LISY +YQ+F+P I++ +LEYIKH  
Sbjct: 77  VIPFLIVQLPQILH-SNSGRHLAVLIGFVVSVCLLISYCLYQIFQPWIQRRKLEYIKHKH 135

Query: 285 LILRIFQRVEKQTLQKILTEDGTPNITAISGLYHEISQRGNKDLLTSEVKELLLGNNLTD 344
           +I+ + + ++ ++L ++L +DG P+   I  L+  I +  +  L   E++ L++G    +
Sbjct: 136 VIVGLLRHLKMRSLGRLLKDDGQPDKEVIKKLFTTIDENKDGHLTHGEMRALVVGIQFEE 195

Query: 345 TNI-QDEKIADMLKVFDRNGDQIITKEEFVTGLTEYI 380
            ++  D+ +  ++  FD + +Q+I + EFV G+  ++
Sbjct: 196 IDLDHDDAVTKIMADFDTSRNQLIDEGEFVNGVCRWL 232


>Medtr2g078250.1 | calcium ion-binding protein, putative | HC |
           chr2:32542664-32542263 | 20130731
          Length = 133

 Score = 86.3 bits (212), Expect = 1e-16,   Method: Composition-based stats.
 Identities = 48/131 (36%), Positives = 78/131 (59%), Gaps = 23/131 (17%)

Query: 10  FTLYLSVVALQV--VECRFLSTSVSQSLVSDGDDDVQIQTQDSYLRLIDQGVVEHESSPG 67
           F L+L VVA+       RF     ++ L+SDG + +++ T             E +S+  
Sbjct: 22  FFLFLGVVAILCSHTHARF----STRDLISDGSNVLRLPT-------------EADST-- 62

Query: 68  EYCKQMYGFLPCSNNILGHLFLILVYEYLLFHGESYLAAGGEQIFKILGPGVFGASAFDI 127
             C+Q YGFLPC+  +LG+LFLILVY +L++   ++L+ G E + +ILGPG+ G     I
Sbjct: 63  --CEQTYGFLPCTTTVLGNLFLILVYGFLMYTAATFLSNGSELLLEILGPGIVGGLFLPI 120

Query: 128 LGALPESLILV 138
           LGALP++++++
Sbjct: 121 LGALPDAMLIL 131