Miyakogusa Predicted Gene

Lj0g3v0129059.2

BLASTP 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Lj0g3v0129059.2 tr|Q93WZ7|Q93WZ7_PHAVU NADH glutamate synthase
(Precursor) OS=Phaseolus vulgaris PE=2 SV=2,92.26,0,Alpha subunit of
glutamate synthase, C-terminal domain,Glutamate synthase, alpha
subunit, C-terminal,CUFF.7971.2
         (440 letters)

Database: Medicago_aa4.0v1 
           62,319 sequences; 21,947,249 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Medtr1g027020.1 | NADH glutamate synthase | HC | chr1:8906141-89...   702   0.0  
Medtr7g089970.1 | ferredoxin-dependent glutamate synthase | HC |...   223   2e-58

>Medtr1g027020.1 | NADH glutamate synthase | HC | chr1:8906141-8918572
            | 20130731
          Length = 2194

 Score =  702 bits (1811), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 347/440 (78%), Positives = 361/440 (82%), Gaps = 2/440 (0%)

Query: 1    MMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMAQLGFRTVNEMVG 60
            MMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINF FMVAEEMREIM+QLGFRTVNEMVG
Sbjct: 1248 MMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFLFMVAEEMREIMSQLGFRTVNEMVG 1307

Query: 61   HSDMLEVDKEVVKSNEKLENIDLSLLLRPAAELRPEAAQYCVQKQDHSLDMALDNQLISL 120
             SDMLEVDKEVVK N KLENIDLSLLLRPAAELRPEAAQYCVQKQDH LDMALDN+LISL
Sbjct: 1308 RSDMLEVDKEVVKGNAKLENIDLSLLLRPAAELRPEAAQYCVQKQDHGLDMALDNKLISL 1367

Query: 121  SSAALEKGLPVYIETPIYNVNRAVGTMLSHEVTKRYHLAGLPTDTIHIKFNGSAGQSFGA 180
            S+AALEKGLPVYIETPI N NRAVGTMLSHEVTKRY+LAGLP DTIHI+F GSAGQSFGA
Sbjct: 1368 SNAALEKGLPVYIETPICNTNRAVGTMLSHEVTKRYNLAGLPADTIHIQFTGSAGQSFGA 1427

Query: 181  FLCPGITLELEGDSNDYXXXXXXXXXXXXYPPKGSNFDPKENIVIGNVALYGATRGEAYF 240
            FLCPGITLELEGDSNDY            YPPKGSNFDPK+NI+IGNVALYGATRGEAYF
Sbjct: 1428 FLCPGITLELEGDSNDYIGKGLSGGKVVVYPPKGSNFDPKDNILIGNVALYGATRGEAYF 1487

Query: 241  NGMAAERFCVRNSGAKAVVEGVGDHGCEYMXXXXXXXXXXXXRNFAAGMSGGIAYVLNAD 300
            NGMAAERFCVRNSGA+AVVEGVGDHGCEYM            RNFAAGMSGGIAYVL+ D
Sbjct: 1488 NGMAAERFCVRNSGAQAVVEGVGDHGCEYMTGGTVVVLGKTGRNFAAGMSGGIAYVLDVD 1547

Query: 301  GKFQSRCNXXXXXXXXXXXXXXXXTLKMLIQQHQRHTNSLLAKEVLDNFGNLLPKFVKVI 360
            G FQSRCN                TL+MLIQQHQRHTNSLLAKEVL +F NLLPKFVKV 
Sbjct: 1548 GTFQSRCNLELVDLDKVEEEEDIITLRMLIQQHQRHTNSLLAKEVLVDFDNLLPKFVKVF 1607

Query: 361  PREYKRVLASMKSEEASKDAVERTXXXXXXXXXXXXXXXXXXFEELKKLATASLKEKQSE 420
            PREYKRVLASMKS+ ASKDAVE                    FEELKKLATASL EK SE
Sbjct: 1608 PREYKRVLASMKSDAASKDAVESAAEDVDEQDDEAQAVEKDAFEELKKLATASLNEKPSE 1667

Query: 421  AESPKRPSQVTEAIKHRGFV 440
            A  PKRPSQVT+A+KHRGFV
Sbjct: 1668 A--PKRPSQVTDAVKHRGFV 1685


>Medtr7g089970.1 | ferredoxin-dependent glutamate synthase | HC |
            chr7:35266109-35288293 | 20130731
          Length = 1612

 Score =  223 bits (569), Expect = 2e-58,   Method: Compositional matrix adjust.
 Identities = 129/365 (35%), Positives = 194/365 (53%), Gaps = 11/365 (3%)

Query: 1    MMRKCHKNTCPVGIATQDPVLREKFAGEPEHVINFFFMVAEEMREIMAQLGFRTVNEMVG 60
            M R CH N CPVG+A+Q   LR +F G P  ++N F  VAEE+R  +AQLG+  +++++G
Sbjct: 1229 MARICHTNNCPVGVASQREELRARFPGVPGDLVNLFLYVAEEVRGTLAQLGYEKLDDIIG 1288

Query: 61   HSDMLEV-DKEVVKSNEKLENIDLSLLLRPAAELRPEAAQYCVQKQD-HSLDMALDNQLI 118
             +++L   D  +VK+    +++DLS +L       P+ +   ++ Q+ H+    LD+ L+
Sbjct: 1289 RTELLRPRDVSLVKT----QHLDLSYILSNVG--LPKLSSTEIRNQEPHTNGPVLDDVLL 1342

Query: 119  SLS--SAALEKGLPVYIETPIYNVNRAVGTMLSHEVTKRYHLAGLPTDTIHIKFNGSAGQ 176
            +    + A+E    V     IYNV+R+    ++  + K+Y   G     ++I F GSAGQ
Sbjct: 1343 ADPKIADAIENEKAVSKTIKIYNVDRSACGRIAGVIAKKYGDTGF-AGQLNITFTGSAGQ 1401

Query: 177  SFGAFLCPGITLELEGDSNDYXXXXXXXXXXXXYPPKGSNFDPKENIVIGNVALYGATRG 236
            SFG FL PG+ + L G++NDY             P     F P++  ++GN  LYGAT G
Sbjct: 1402 SFGCFLTPGMNIRLVGEANDYVGKGIAGGELVVTPVDKIGFQPEDAAIVGNTCLYGATGG 1461

Query: 237  EAYFNGMAAERFCVRNSGAKAVVEGVGDHGCEYMXXXXXXXXXXXXRNFAAGMSGGIAYV 296
            + +  G A ERF VRNS A+AVVEG GDH CEYM            RN AAGM+GG+AY+
Sbjct: 1462 QVFVRGKAGERFAVRNSLAEAVVEGAGDHCCEYMTGGCVVILGNVGRNVAAGMTGGLAYI 1521

Query: 297  LNADGKFQSRCNXXXXXXXXXXXXXXXXTLKMLIQQHQRHTNSLLAKEVLDNFGNLLPKF 356
            L+ D     + N                 LK LI+ H   T S   + +L ++   L  F
Sbjct: 1522 LDEDNTLIPKINREIVKIQRVTAPVGQIQLKKLIEAHVEKTGSNKGEAILKDWDKYLSLF 1581

Query: 357  VKVIP 361
             +++P
Sbjct: 1582 WQLVP 1586